FastQCFastQC Report
Wed 25 May 2016
SRR1295112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983125
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT76150.2552692227110832No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55730.1868175151896082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT31300.017.10141812-13
GTACTAG6750.015.4423621
GTATAGG11000.014.8908491
GAACCGA2952.440041E-514.3285236
CTAGGAC12450.013.9778473
GTATCAA117050.013.9515571
GTCCTAA10350.013.9077181
ATAGGAC9850.013.84744553
TATACAG19800.013.5399675
AGTACTT41550.013.39118412-13
CTAGACT12800.013.228194
GTATAAG14300.013.190031
ATTATAC17200.013.1256473
CTGTCGC5452.8485374E-912.9239579
GTCTAGC6952.1827873E-1112.855411
GTACATG143350.012.7076891
GTCCTAG12500.012.7068591
CGTACCC2600.002170289212.6628843
TACATGG143600.012.6433782
CAACGCA123700.012.6233995
Produced by FastQC (version 0.11.5)