FastQCFastQC Report
Wed 25 May 2016
SRR1295111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3289634
Sequences flagged as poor quality0
Sequence length100
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG2151.4975338E-518.666935
GTACATG93850.018.524791
TACATGG97900.017.056822
GTATAAG10700.016.7685471
GTATCAA65700.016.6682171
CATGGGG50300.016.2092764
ACATGGG101250.015.3878963
GTACTAT9250.014.7150641
GTATAGG8850.014.6810541
GTACTAG5601.999797E-814.3627161
TAGACTG12500.014.0468655
TATCAAC72900.013.9982392
TATACAG14800.013.8977185
TAGACCG2200.00691397113.6820125
ATCAACG72050.013.4085623
TCAACGC71450.013.3651124
GTCTAGA10450.013.0252971
ATAGGAC8153.6379788E-1213.0186533
GTCTAAG8151.05501385E-1012.9054321
GTATAAA18700.012.9034021
Produced by FastQC (version 0.11.5)