##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295111_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3289634
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.709930952805085	27.0	22.0	33.0	6.0	33.0
2	21.802509032919772	27.0	6.0	33.0	2.0	33.0
3	24.192425661942938	27.0	15.0	33.0	15.0	33.0
4	24.482968318056052	33.0	6.0	37.0	2.0	37.0
5	26.29706313833089	33.0	15.0	37.0	2.0	37.0
6	26.714387375616862	33.0	15.0	37.0	6.0	37.0
7	26.53993422976538	33.0	15.0	37.0	6.0	37.0
8	27.307670397375514	33.0	15.0	37.0	6.0	37.0
9	28.39073799699298	33.0	22.0	37.0	15.0	37.0
10-11	27.89337066068748	33.0	18.5	37.0	6.0	37.0
12-13	26.69866647779054	33.0	15.0	37.0	4.0	37.0
14-15	28.326885300917972	33.0	18.5	37.0	6.0	40.0
16-17	28.71918334988026	33.0	22.0	37.0	6.0	40.0
18-19	28.83195410188489	33.0	22.0	37.0	6.0	40.0
20-21	28.332963028713834	33.0	18.5	37.0	6.0	40.0
22-23	29.066374709162176	33.0	22.0	37.0	6.0	40.0
24-25	29.15358684279163	33.0	22.0	37.0	6.0	40.0
26-27	27.77751430706273	33.0	15.0	37.0	4.0	40.0
28-29	28.466082549000895	33.0	22.0	37.0	6.0	40.0
30-31	29.16963680458069	33.0	22.0	37.0	6.0	40.0
32-33	27.992317078434866	33.0	18.5	37.0	6.0	40.0
34-35	27.520657769222957	33.0	15.0	37.0	6.0	40.0
36-37	27.79201561632692	33.0	18.5	37.0	6.0	40.0
38-39	27.905201156116455	33.0	18.5	37.0	6.0	40.0
40-41	27.018395967454133	33.0	15.0	37.0	6.0	40.0
42-43	27.553830000541094	33.0	15.0	37.0	6.0	40.0
44-45	27.331661972122127	33.0	15.0	37.0	6.0	40.0
46-47	27.06588270914029	33.0	15.0	37.0	6.0	40.0
48-49	26.876327883284283	33.0	15.0	37.0	6.0	40.0
50-51	26.79731529404183	33.0	15.0	37.0	6.0	40.0
52-53	27.02380872765785	33.0	18.5	37.0	6.0	40.0
54-55	26.975726630986912	33.0	22.0	37.0	6.0	40.0
56-57	26.938903081619415	33.0	18.5	37.0	6.0	40.0
58-59	26.747011977624258	33.0	15.0	37.0	6.0	40.0
60-61	26.726122723682938	33.0	18.5	37.0	6.0	40.0
62-63	26.645442471715697	27.0	18.5	37.0	6.0	37.0
64-65	26.20505959021581	27.0	15.0	37.0	6.0	37.0
66-67	26.4396055913819	27.0	18.5	37.0	6.0	37.0
68-69	26.033158855969994	27.0	18.5	37.0	6.0	37.0
70-71	26.140488729141296	27.0	18.5	35.0	6.0	37.0
72-73	26.16061072447573	27.0	18.5	33.0	6.0	37.0
74-75	26.26451012483456	27.0	22.0	33.0	6.0	37.0
76-77	26.02371829206532	27.0	22.0	33.0	6.0	37.0
78-79	25.612814981849045	27.0	18.5	33.0	6.0	37.0
80-81	25.44109314288459	27.0	18.5	33.0	6.0	37.0
82-83	25.52878998089149	27.0	22.0	33.0	6.0	37.0
84-85	25.48223495379729	27.0	22.0	33.0	6.0	37.0
86-87	25.29525746633212	27.0	18.5	33.0	6.0	37.0
88-89	25.236416422009256	27.0	22.0	33.0	6.0	37.0
90-91	25.368953202696712	27.0	22.0	33.0	6.0	37.0
92-93	25.300738623202463	27.0	22.0	33.0	6.0	37.0
94-95	25.170138988106274	27.0	22.0	33.0	6.0	37.0
96-97	25.249761523622382	27.0	22.0	33.0	6.0	37.0
98-99	25.3127802363424	27.0	22.0	33.0	6.0	37.0
100	24.995690098047383	27.0	15.0	33.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.227319400812856
1101	2	11.068795182300512
1101	3	8.188596781355269
1101	4	12.444922229836092
1101	5	10.575060172164964
1101	6	9.63286549876091
1101	7	9.302888938565701
1101	8	8.817654875097148
1101	9	7.126444170207286
1101	10-11	7.550916934379991
1101	12-13	8.913837401823663
1101	14-15	7.6289044014928
1101	16-17	7.9116283415960496
1101	18-19	7.992987829990319
1101	20-21	6.883471281661137
1101	22-23	7.06361066696979
1101	24-25	7.112065196171155
1101	26-27	8.153800693053967
1101	28-29	7.949141289780165
1101	30-31	5.309785844789268
1101	32-33	6.562791759321758
1101	34-35	8.462754790438229
1101	36-37	7.848277443805696
1101	38-39	7.866236825536017
1101	40-41	8.775360116528503
1101	42-43	8.298404914959455
1101	44-45	8.124832214936358
1101	46-47	6.935109814666127
1101	48-49	5.092228501776056
1101	50-51	6.300809059100715
1101	52-53	5.546561011479792
1101	54-55	3.7035816951167
1101	56-57	5.805067574733268
1101	58-59	5.829103747137154
1101	60-61	6.3835946031955295
1101	62-63	5.965392195804952
1101	64-65	6.38203811502018
1101	66-67	5.461289912120112
1101	68-69	6.321403043075424
1101	70-71	5.862288923595212
1101	72-73	5.222912426475791
1101	74-75	4.571844518281193
1101	76-77	4.788248401247426
1101	78-79	4.884689321775035
1101	80-81	4.776165191753947
1101	82-83	4.312745643652438
1101	84-85	4.02833353446912
1101	86-87	3.854436431786091
1101	88-89	3.840667956962289
1101	90-91	3.6829418974667263
1101	92-93	3.5656002986077944
1101	94-95	3.498152351715717
1101	96-97	3.5296980134136327
1101	98-99	3.3066910702077017
1101	100	3.4909566703422
1102	1	6.706521072942461
1102	2	10.261699754026598
1102	3	8.017495752738824
1102	4	12.20581499172825
1102	5	10.353946864893214
1102	6	9.336331587746084
1102	7	6.493120072292218
1102	8	7.596228103323067
1102	9	6.205877542157257
1102	10-11	7.180741529004976
1102	12-13	8.850257508042013
1102	14-15	5.866897949617556
1102	16-17	6.973565187847356
1102	18-19	7.17452139018642
1102	20-21	6.559257892983492
1102	22-23	6.278941385374775
1102	24-25	6.857048747556927
1102	26-27	8.174015406072286
1102	28-29	7.363285662847176
1102	30-31	4.021196642351718
1102	32-33	4.084028211938506
1102	34-35	4.886448190023149
1102	36-37	4.9310703236207765
1102	38-39	4.450784596282357
1102	40-41	6.000221563065722
1102	42-43	6.644711551253728
1102	44-45	6.154237242328126
1102	46-47	5.244257891262613
1102	48-49	3.3746817053489835
1102	50-51	4.642530454817059
1102	52-53	5.458497080348696
1102	54-55	4.987426991235608
1102	56-57	5.858060337502469
1102	58-59	5.701804090540847
1102	60-61	5.594985806734972
1102	62-63	3.9950032095993038
1102	64-65	5.848872789304927
1102	66-67	3.6048573917028612
1102	68-69	5.6074748421136995
1102	70-71	4.884370650006041
1102	72-73	4.779795170898769
1102	74-75	3.9923598988916034
1102	76-77	3.0213580743561366
1102	78-79	4.079118200853404
1102	80-81	4.19295256530971
1102	82-83	3.5736187716558234
1102	84-85	2.7547531742580205
1102	86-87	3.014850146725152
1102	88-89	3.100408339147396
1102	90-91	2.9282942869010213
1102	92-93	2.8643999603866526
1102	94-95	2.7447552903009083
1102	96-97	2.9688999725767395
1102	98-99	2.7920989049465703
1102	100	3.0301610613169103
1103	1	6.520447586871754
1103	2	10.736873790790277
1103	3	7.728684998664203
1103	4	11.595295453878432
1103	5	10.161504284321811
1103	6	8.758261172725565
1103	7	7.813201151828288
1103	8	7.776931141979553
1103	9	6.987126113023244
1103	10-11	7.319037397854935
1103	12-13	8.596674033912514
1103	14-15	8.295009412542644
1103	16-17	7.783669601965926
1103	18-19	7.065038887550465
1103	20-21	8.149255900135511
1103	22-23	6.632958839864795
1103	24-25	5.083793005614066
1103	26-27	5.653930237625026
1103	28-29	6.749416750958055
1103	30-31	5.814036164893928
1103	32-33	7.786507759442916
1103	34-35	6.912098406298764
1103	36-37	3.830804703186068
1103	38-39	6.094213715999324
1103	40-41	6.556946273745481
1103	42-43	7.262223563919029
1103	44-45	5.330668559735614
1103	46-47	5.9148007795742
1103	48-49	5.286535419191896
1103	50-51	6.187874058330824
1103	52-53	5.799409217189865
1103	54-55	4.714861203433426
1103	56-57	5.640833357675493
1103	58-59	5.2067512760996095
1103	60-61	4.775061455561811
1103	62-63	2.5571571209802926
1103	64-65	4.758197015349467
1103	66-67	3.7218012442035295
1103	68-69	4.824403728405446
1103	70-71	3.4759322091594846
1103	72-73	4.1647938687988955
1103	74-75	2.975873210480927
1103	76-77	3.249551077316031
1103	78-79	2.7385075715898175
1103	80-81	3.916593498565973
1103	82-83	3.2555770663496055
1103	84-85	3.022095477259839
1103	86-87	2.5171977375227996
1103	88-89	2.8741600699874716
1103	90-91	2.5897139409870746
1103	92-93	2.1309432980814904
1103	94-95	2.6332252753108847
1103	96-97	2.7702207305894184
1103	98-99	2.484785668942557
1103	100	2.66119121286863
1104	1	6.393317502498512
1104	2	9.631463056287693
1104	3	7.248836027714152
1104	4	11.963676281419271
1104	5	9.279687144663225
1104	6	7.975079055551753
1104	7	3.9297746590101355
1104	8	6.351381593256221
1104	9	6.552202414199034
1104	10-11	7.080376633309406
1104	12-13	7.433632628408645
1104	14-15	6.955358535288632
1104	16-17	6.938801905564127
1104	18-19	6.632731593281392
1104	20-21	7.393653582559441
1104	22-23	6.337109035618301
1104	24-25	5.929742702654682
1104	26-27	8.157699184445853
1104	28-29	7.05049883065724
1104	30-31	6.2126617989581945
1104	32-33	7.4268773351100315
1104	34-35	7.889664726101177
1104	36-37	6.522160695488747
1104	38-39	7.151885676285712
1104	40-41	6.89333199670525
1104	42-43	7.140610095666517
1104	44-45	6.505924972323289
1104	46-47	6.554455392431322
1104	48-49	5.373708583567918
1104	50-51	5.402028930505995
1104	52-53	5.7831750371725335
1104	54-55	4.636788798353322
1104	56-57	5.85847427885545
1104	58-59	3.724965128111222
1104	60-61	4.095573355730682
1104	62-63	3.7087473810573286
1104	64-65	2.5848762974179422
1104	66-67	3.0349986951386576
1104	68-69	3.9044890503709517
1104	70-71	2.330674745068709
1104	72-73	2.2895355785271185
1104	74-75	2.9989936486968176
1104	76-77	3.654201883415695
1104	78-79	4.300770410205441
1104	80-81	3.0118655097672473
1104	82-83	2.907429609247771
1104	84-85	2.7819312609379807
1104	86-87	0.5947023704612491
1104	88-89	0.06665763492767596
1104	90-91	2.1662864915382833
1104	92-93	2.2024568933049764
1104	94-95	2.2424960934909777
1104	96-97	2.6682926454951463
1104	98-99	2.313300713342869
1104	100	0.6784851182618148
1105	1	5.7325461255530215
1105	2	-2.4598213689213146
1105	3	0.598115632013716
1105	4	8.231543499529767
1105	5	8.328318093220087
1105	6	7.810114501161706
1105	7	5.114463998371864
1105	8	5.697321807142082
1105	9	4.700569271694185
1105	10-11	4.842714570063116
1105	12-13	4.977213085905838
1105	14-15	5.647083587062621
1105	16-17	5.87776898180573
1105	18-19	6.03061337353256
1105	20-21	6.804124806908945
1105	22-23	1.9006000573670114
1105	24-25	3.87596557374221
1105	26-27	6.7155803438610135
1105	28-29	5.723926057577319
1105	30-31	5.1672432936051536
1105	32-33	6.991579095121157
1105	34-35	5.965211823093636
1105	36-37	6.112731453995135
1105	38-39	4.055601851283665
1105	40-41	5.115517119392074
1105	42-43	5.44233917305117
1105	44-45	6.086855316684911
1105	46-47	5.186305222418525
1105	48-49	4.803281173344047
1105	50-51	5.71530038063548
1105	52-53	5.346578238132235
1105	54-55	5.062628041907875
1105	56-57	4.998742635217674
1105	58-59	2.0035624552550573
1105	60-61	4.288370312298294
1105	62-63	-0.7094654030168925
1105	64-65	-0.3498270304005615
1105	66-67	0.7190864817423908
1105	68-69	2.5924511974497513
1105	70-71	2.771164205493463
1105	72-73	2.4664557054212786
1105	74-75	2.4902865416373814
1105	76-77	1.7176210178563593
1105	78-79	3.129639097693296
1105	80-81	1.4464629909412814
1105	82-83	0.7942926899578246
1105	84-85	1.1753197504420214
1105	86-87	-0.3414338311344167
1105	88-89	1.5466552321351692
1105	90-91	1.253232314363082
1105	92-93	1.576205294799621
1105	94-95	0.9603860330203702
1105	96-97	1.9641690075769205
1105	98-99	1.929454050798082
1105	100	1.9724208471969575
1106	1	5.853661355882053
1106	2	9.987236418590474
1106	3	6.383685826147495
1106	4	11.268134039471057
1106	5	9.498339461493618
1106	6	9.025954510846145
1106	7	6.201080672131084
1106	8	7.114961884464261
1106	9	6.549930030026829
1106	10-11	5.339625447063856
1106	12-13	7.602131138176027
1106	14-15	7.453677970109926
1106	16-17	7.351490292258411
1106	18-19	6.78867756455584
1106	20-21	7.856774197489688
1106	22-23	5.762134591705827
1106	24-25	4.617856916309453
1106	26-27	7.258259732277395
1106	28-29	5.9086648779784525
1106	30-31	5.037698295050966
1106	32-33	6.89473267843994
1106	34-35	6.318210317102608
1106	36-37	5.465378166716338
1106	38-39	6.580911980528434
1106	40-41	6.2069609689760625
1106	42-43	5.674233241110162
1106	44-45	3.93172517096869
1106	46-47	5.3925909371357506
1106	48-49	5.22145900087618
1106	50-51	6.04263295603581
1106	52-53	5.087495966735762
1106	54-55	4.729622702801983
1106	56-57	4.340987170612532
1106	58-59	4.437442008037408
1106	60-61	4.285163764286946
1106	62-63	4.539080623812126
1106	64-65	4.6161672611696645
1106	66-67	2.6624611668034532
1106	68-69	2.8262419568244965
1106	70-71	2.4435434261863804
1106	72-73	2.486156822605693
1106	74-75	2.3978202841197707
1106	76-77	3.0274955464862785
1106	78-79	3.682912557340277
1106	80-81	3.2026478299345413
1106	82-83	1.5412414403733017
1106	84-85	2.3178048517261054
1106	86-87	3.04019257876444
1106	88-89	3.1186819280131566
1106	90-91	2.864166189123601
1106	92-93	2.5294280842323786
1106	94-95	1.127053629095986
1106	96-97	1.1468445691519484
1106	98-99	1.6358899812369323
1106	100	2.4876661745644135
1107	1	5.828558373922441
1107	2	10.134245753068
1107	3	5.820058147166204
1107	4	10.945295321400355
1107	5	9.085738475323403
1107	6	7.619183896549615
1107	7	3.428636897156178
1107	8	5.88336414485542
1107	9	5.390047172766806
1107	10-11	4.276899433940095
1107	12-13	5.717958686280689
1107	14-15	5.707785750119225
1107	16-17	6.482248480425241
1107	18-19	5.830615162893519
1107	20-21	5.095580722583723
1107	22-23	5.280588121322413
1107	24-25	4.162200602779723
1107	26-27	6.690514282261724
1107	28-29	5.988425385956951
1107	30-31	4.662963632134435
1107	32-33	4.538819618755568
1107	34-35	6.174759034017288
1107	36-37	5.949648515197495
1107	38-39	5.898983240384329
1107	40-41	7.161959111650297
1107	42-43	5.675347588256965
1107	44-45	3.5617374914292945
1107	46-47	2.999904295994316
1107	48-49	3.391604988884403
1107	50-51	4.174419224548725
1107	52-53	3.2832830896455825
1107	54-55	4.707825078532473
1107	56-57	4.6904521734430595
1107	58-59	2.4874506153924827
1107	60-61	2.4120697359091103
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2103	3	7.578031201473511
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2103	6	8.952408045640102
2103	7	6.317024731266539
2103	8	7.630361810300236
2103	9	6.4934988938747615
2103	10-11	7.3875863608632955
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2207	5	10.323615618361718
2207	6	9.0955148972017
2207	7	5.633053217226468
2207	8	6.6197221169606735
2207	9	6.007707902011216
2207	10-11	6.738569948213431
2207	12-13	8.741014186005238
2207	14-15	8.073903217738678
2207	16-17	7.20739788442031
2207	18-19	4.096478117343736
2207	20-21	7.2605790100405905
2207	22-23	6.132340713768581
2207	24-25	4.175089961734141
2207	26-27	3.7330263357144347
2207	28-29	4.890265867957478
2207	30-31	5.365595554047189
2207	32-33	6.688574978974344
2207	34-35	6.031767371018038
2207	36-37	6.461262552941598
2207	38-39	6.721170091895562
2207	40-41	3.803999424276533
2207	42-43	5.532133162505119
2207	44-45	3.9568926916232705
2207	46-47	5.402256188566756
2207	48-49	2.3479521303469113
2207	50-51	5.138249194516572
2207	52-53	2.7154208898652286
2207	54-55	3.045753137700302
2207	56-57	5.114953238391287
2207	58-59	3.6270393474978206
2207	60-61	3.467632711995492
2207	62-63	4.15306704808993
2207	64-65	3.4591063046079924
2207	66-67	3.3869326124364854
2207	68-69	3.2231463757354497
2207	70-71	3.3728259290316025
2207	72-73	3.691296847049685
2207	74-75	3.4036240359842083
2207	76-77	3.9188793112665152
2207	78-79	4.197167082509164
2207	80-81	4.134418661686574
2207	82-83	3.7224193889499126
2207	84-85	3.092860542638064
2207	86-87	3.3170898620869593
2207	88-89	3.2184236194868383
2207	90-91	1.265383446595024
2207	92-93	-0.844963463200763
2207	94-95	1.1316420782136802
2207	96-97	-1.1078009568018672
2207	98-99	0.5449258937835602
2207	100	1.8548137522294859
2208	1	0.8551025647364732
2208	2	7.9429547196132795
2208	3	6.12732133344074
2208	4	11.100299453698156
2208	5	9.701215056398166
2208	6	8.739016284486041
2208	7	7.118204725325395
2208	8	7.301726712670238
2208	9	6.288395233592766
2208	10-11	6.348698908352521
2208	12-13	6.494642812175417
2208	14-15	7.3638046560307195
2208	16-17	6.773074371465469
2208	18-19	6.270674375848209
2208	20-21	6.286521363694369
2208	22-23	5.436734033247777
2208	24-25	5.951221154265458
2208	26-27	7.666384234749785
2208	28-29	6.768677179037731
2208	30-31	5.704454607777954
2208	32-33	8.045957162256283
2208	34-35	6.105093162454406
2208	36-37	6.499838548405986
2208	38-39	6.873342960802297
2208	40-41	7.196031021505615
2208	42-43	6.633608586002541
2208	44-45	5.3779173533187254
2208	46-47	4.401494460757355
2208	48-49	4.1543185004569025
2208	50-51	5.402455260685461
2208	52-53	5.0263585240806385
2208	54-55	6.069166723123743
2208	56-57	6.200745645983471
2208	58-59	5.4387576066860035
2208	60-61	4.731849014127878
2208	62-63	2.896192449574542
2208	64-65	3.0099111177667766
2208	66-67	2.457062823963316
2208	68-69	4.842887097864548
2208	70-71	4.820013722577382
2208	72-73	4.17762225902295
2208	74-75	1.8015254821866833
2208	76-77	2.9838139106927066
2208	78-79	3.7910892805070873
2208	80-81	3.1487025367425545
2208	82-83	0.9937817852537876
2208	84-85	1.573983869499795
2208	86-87	0.8721960505032875
2208	88-89	0.39712785857286903
2208	90-91	1.584322235002297
2208	92-93	0.5295458125323051
2208	94-95	-1.3453477688275157
2208	96-97	0.9164974077273804
2208	98-99	0.03565999383023666
2208	100	1.3917171381562028
2209	1	5.054511432814586
2209	2	9.368914544838699
2209	3	5.240230665113852
2209	4	-3.166563779255714
2209	5	0.6670045879871793
2209	6	4.031430855182631
2209	7	2.008627821134308
2209	8	4.824937110171955
2209	9	4.755614006987134
2209	10-11	4.766985702569656
2209	12-13	6.937681668371283
2209	14-15	6.29706014577172
2209	16-17	5.962043422642246
2209	18-19	4.980738010799072
2209	20-21	5.54971487394123
2209	22-23	5.1726170426329965
2209	24-25	5.0663019441269235
2209	26-27	6.618264465159029
2209	28-29	5.9810331390202265
2209	30-31	3.787152561790549
2209	32-33	5.868114944333204
2209	34-35	5.327975004584818
2209	36-37	5.9155599923988795
2209	38-39	6.5468174649638975
2209	40-41	6.934638196845675
2209	42-43	6.569379332348134
2209	44-45	6.228528166237407
2209	46-47	6.380281313920435
2209	48-49	4.7904443246729045
2209	50-51	4.349428946669416
2209	52-53	4.479314438557125
2209	54-55	5.099338374176618
2209	56-57	4.719761412567049
2209	58-59	2.7363480555097794
2209	60-61	3.874711836101831
2209	62-63	4.020767944015255
2209	64-65	4.834098739024764
2209	66-67	4.301347691603237
2209	68-69	3.4604699202680536
2209	70-71	3.3952424122409397
2209	72-73	0.5147019082047173
2209	74-75	2.0383850677300543
2209	76-77	3.240461555328441
2209	78-79	3.3583045601367836
2209	80-81	3.4983802641614687
2209	82-83	2.821203224934802
2209	84-85	2.794269468980019
2209	86-87	1.2420751932742036
2209	88-89	1.7548721008858728
2209	90-91	2.2247024614479507
2209	92-93	2.2020839478535024
2209	94-95	0.6146183409295531
2209	96-97	-0.01922673694923205
2209	98-99	0.16033269147967744
2209	100	0.05868472329417784
2210	1	1.74793506177242
2210	2	-16.45090647826802
2210	3	-7.195706332280119
2210	4	-5.287627213971822
2210	5	0.21615920739419892
2210	6	3.2381591867255075
2210	7	-4.794374056090817
2210	8	-4.885258248659419
2210	9	-1.4771408103963566
2210	10-11	-0.4654053969578129
2210	12-13	3.380435912079612
2210	14-15	-2.82623520727412
2210	16-17	-0.07135733784808451
2210	18-19	-0.2557937940737105
2210	20-21	2.538805887114979
2210	22-23	2.770143685600118
2210	24-25	2.504067104249124
2210	26-27	4.188242980634065
2210	28-29	3.5739095130966305
2210	30-31	2.809187090498355
2210	32-33	4.614332273752407
2210	34-35	2.3364999223312175
2210	36-37	4.192855771115589
2210	38-39	3.33723611172859
2210	40-41	4.012241823506855
2210	42-43	2.6559036809073078
2210	44-45	3.4991778743313873
2210	46-47	2.800420243799671
2210	48-49	2.888576899831424
2210	50-51	2.137056866229617
2210	52-53	2.422842863234404
2210	54-55	2.9434183684944983
2210	56-57	2.990481854417901
2210	58-59	2.1517528238235535
2210	60-61	2.9787239108907286
2210	62-63	2.350343225367311
2210	64-65	2.836567225184801
2210	66-67	2.751103333303295
2210	68-69	3.324063065357233
2210	70-71	2.4981707605062518
2210	72-73	1.1363618114233738
2210	74-75	0.4921485206808356
2210	76-77	-0.5847130741217939
2210	78-79	0.3879115033818685
2210	80-81	1.1336211950450235
2210	82-83	1.0888388822801893
2210	84-85	-0.20508450010571977
2210	86-87	-0.21028492633121587
2210	88-89	-1.465452941703166
2210	90-91	-1.2558652803685675
2210	92-93	-0.8152364442593267
2210	94-95	-0.008452371460844432
2210	96-97	0.37643359340375326
2210	98-99	0.13400186755238863
2210	100	0.19655854754059732
2211	1	1.2496547808235476
2211	2	-16.45090647826802
2211	3	-7.543919702731747
2211	4	-18.552966877960134
2211	5	-20.66276701612662
2211	6	-12.343390493485941
2211	7	-10.857389034285537
2211	8	-18.46851652661725
2211	9	-14.803380846620648
2211	10-11	-9.61558669034299
2211	12-13	-21.545422378643543
2211	14-15	-16.971268249906217
2211	16-17	-15.3202831111441
2211	18-19	-18.057493462540634
2211	20-21	-7.833400462623516
2211	22-23	-3.5686905835346003
2211	24-25	-1.863343706647651
2211	26-27	-16.469753903681372
2211	28-29	-3.880160626554723
2211	30-31	-4.093400031224164
2211	32-33	-0.37964578149565753
2211	34-35	-4.614439193951643
2211	36-37	-5.769585030770241
2211	38-39	-1.9580579269726925
2211	40-41	-4.477880228246001
2211	42-43	-1.0573587543769065
2211	44-45	0.360635724801309
2211	46-47	0.9184626857072402
2211	48-49	0.7750183316021655
2211	50-51	-3.9037928680732783
2211	52-53	-0.694716016821193
2211	54-55	-0.33061020434016086
2211	56-57	-5.5877567125047385
2211	58-59	-1.5129231830198648
2211	60-61	-0.5590517497201439
2211	62-63	0.08867831984252561
2211	64-65	-2.8517273705487867
2211	66-67	-2.0183792584700377
2211	68-69	0.3775461720416011
2211	70-71	0.09089923240029307
2211	72-73	-0.8803871690162346
2211	74-75	-1.4723587485692846
2211	76-77	-1.3501384581184226
2211	78-79	-0.7593917091601234
2211	80-81	-0.49702638617327466
2211	82-83	-1.251875666385029
2211	84-85	-1.3735270564713957
2211	86-87	-1.6797506857143176
2211	88-89	-2.3816568116593295
2211	90-91	-2.0139610798249024
2211	92-93	-1.9028050874236087
2211	94-95	-1.9256642506905663
2211	96-97	-2.7326045942555304
2211	98-99	-2.6437207245561893
2211	100	-1.9908245310127803
2212	1	-20.58058940864394
2212	2	-16.45090647826802
2212	3	-19.44241926142548
2212	4	-18.552966877960134
2212	5	-20.66276701612662
2212	6	-21.073016854180263
2212	7	-21.610845933373504
2212	8	-22.002497109141522
2212	9	-23.412677698635946
2212	10-11	-10.838696018256403
2212	12-13	-21.545422378643543
2212	14-15	-22.84358363678471
2212	16-17	-23.192157191756046
2212	18-19	-17.808457318209456
2212	20-21	-22.87856080390036
2212	22-23	-17.934263302954065
2212	24-25	-24.34627986434109
2212	26-27	-16.48731392250575
2212	28-29	-17.4212188121706
2212	30-31	-5.937106406077795
2212	32-33	-16.437220645323663
2212	34-35	-13.123563039844914
2212	36-37	-16.88252331880668
2212	38-39	-22.805660162924443
2212	40-41	-9.533673310697015
2212	42-43	-13.448088495020102
2212	44-45	-16.30441582246652
2212	46-47	-16.16080570703541
2212	48-49	-16.83733792152595
2212	50-51	-8.412181716328387
2212	52-53	-2.0558926694340407
2212	54-55	-22.480065926611417
2212	56-57	-22.36077818940971
2212	58-59	-11.760775477871057
2212	60-61	-5.972547882869218
2212	62-63	-3.012684030110414
2212	64-65	-16.33803185839323
2212	66-67	-16.65435081795144
2212	68-69	-13.845063023421327
2212	70-71	-8.9256154678455
2212	72-73	-16.562295531447653
2212	74-75	-6.002812104313705
2212	76-77	-10.209798743133113
2212	78-79	-4.501203148310847
2212	80-81	-2.1445093434292737
2212	82-83	-2.647074658589201
2212	84-85	-4.900445859634736
2212	86-87	-4.755339914559457
2212	88-89	-3.0293447448339563
2212	90-91	-3.946716011847329
2212	92-93	-5.097404521402904
2212	94-95	-3.45595109097286
2212	96-97	-5.119236574718499
2212	98-99	-3.581121386691411
2212	100	-3.290853746308116
2213	1	-20.58058940864394
2213	2	-16.45090647826802
2213	3	-19.44241926142548
2213	4	-18.552966877960134
2213	5	-20.66276701612662
2213	6	-21.073016854180263
2213	7	-10.19532744171288
2213	8	-9.818668247647686
2213	9	-4.4833811069028044
2213	10-11	-17.040871363286726
2213	12-13	-15.61975160271896
2213	14-15	-22.84358363678471
2213	16-17	-17.215181122140383
2213	18-19	-17.868829859949674
2213	20-21	-16.966668128048294
2213	22-23	-19.02522794330492
2213	24-25	-7.225902779496998
2213	26-27	-22.44871830497587
2213	28-29	-23.308669011373787
2213	30-31	-18.70888802506956
2213	32-33	-22.52262901185753
2213	34-35	-17.178081008579113
2213	36-37	-22.89123845824891
2213	38-39	-10.846813172351563
2213	40-41	-16.32111148403701
2213	42-43	-22.341265785856756
2213	44-45	-16.238375234997687
2213	46-47	-16.003697374592882
2213	48-49	-5.602737165738368
2213	50-51	-22.395996457364244
2213	52-53	-16.896552510129276
2213	54-55	-16.752908566205324
2213	56-57	-16.50562953096156
2213	58-59	-17.009786142969908
2213	60-61	-18.263231955859347
2213	62-63	-16.533017670169468
2213	64-65	-16.58846684794915
2213	66-67	-8.063541466524658
2213	68-69	-21.714833939755987
2213	70-71	-16.514125098647888
2213	72-73	-8.822609063396722
2213	74-75	-3.9342076244810436
2213	76-77	-2.7975678624499025
2213	78-79	-4.175751690906239
2213	80-81	-2.7638291996100612
2213	82-83	-2.914119243122716
2213	84-85	-2.6452917119036883
2213	86-87	-2.7700428865727815
2213	88-89	-2.438517155987025
2213	90-91	-2.3756949835601873
2213	92-93	-3.3360032447112644
2213	94-95	-3.3199513986616154
2213	96-97	-2.850761706966882
2213	98-99	-3.284552946890063
2213	100	-2.886865276691296
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	885.0
3	962.0
4	4437.0
5	14445.0
6	23938.0
7	28751.0
8	27344.0
9	28677.0
10	35920.0
11	45439.0
12	59993.0
13	75965.0
14	68996.0
15	74807.0
16	80847.0
17	82117.0
18	81242.0
19	80544.0
20	79285.0
21	78962.0
22	81499.0
23	87364.0
24	92903.0
25	100044.0
26	107029.0
27	113050.0
28	119650.0
29	124288.0
30	130048.0
31	141442.0
32	159131.0
33	184552.0
34	210991.0
35	229758.0
36	224628.0
37	166719.0
38	42904.0
39	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.345114089462555	20.921547075404355	13.903990525506266	24.829348309626823
2	15.124096289116343	21.53796778128721	38.94103637640938	24.39689955318707
3	17.42056505036802	26.44827053307529	30.834901911844486	25.296262504712203
4	12.4433190781367	17.949522284112096	38.301765806278325	31.30539283147288
5	13.401483035041032	37.17935327068946	35.92298267085804	13.496181023411467
6	30.099110971627567	37.690831294282575	18.539961250755194	13.670096483334666
7	25.659148471860732	31.804181928476705	24.787698843589908	17.748970756072655
8	24.884629014469812	35.21582173882804	22.08022150169405	17.8193277450081
9	24.928801210938023	16.97340188165264	22.025116366368763	36.07268054104057
10-11	23.502368402128226	27.112957341428345	29.02988738985718	20.35478686658625
12-13	24.169936224683017	25.017951009649614	28.712038201229724	22.100074564437644
14-15	21.71299656720109	26.357367720053066	26.266002934460392	25.66363277828545
16-17	21.58240291629338	29.655752272927593	27.063230208000338	21.698614602778687
18-19	21.52814214873723	28.90657079934697	28.272566684015644	21.292720367900156
20-21	22.848824243421728	28.25265496739153	27.384631843150164	21.51388894603658
22-23	22.074489209544595	28.238559570433402	27.85817394964658	21.828777270375426
24-25	22.258288233479835	28.086913111697026	27.396270336948845	22.258528317874298
26-27	22.202442163920075	28.133949561243533	27.49903602331294	22.164572251523452
28-29	22.031284877107854	27.9625921403582	27.9639851998765	22.042137782657445
30-31	22.582725561610193	28.256870657346205	27.31159393109805	21.84880984994555
32-33	21.873948390181184	28.282241051598216	27.775611703300402	22.068198854920198
34-35	22.06963602709542	27.842384313234025	27.4745554142942	22.613424245376358
36-37	22.508551506915513	28.21581047538867	27.252032314111425	22.023605703584398
38-39	22.247592466876903	27.996819808455907	27.452566203832724	22.303021520834466
40-41	22.432649739738967	27.91655490326579	27.054931210073264	22.595864146921983
42-43	22.193186391715614	28.070089274642918	27.608487647642256	22.128236685999212
44-45	22.543268478895907	27.64703260764754	27.485829720030114	22.32386919342645
46-47	22.454195777354954	28.018471195405443	27.53585054386354	21.991482483376068
48-49	22.38203187822205	27.799382150592063	27.553165541625162	22.265420429560727
50-51	22.566016551833027	27.817420114721557	27.330211651582108	22.2863516818633
52-53	22.080724372532632	27.852738798047238	27.500747230487764	22.565789598932366
54-55	22.53964921661909	27.758647148852106	27.2681866024907	22.433517032038104
56-57	22.542552789712975	27.734986709050673	27.246820601903675	22.475639899332673
58-59	22.35999364956543	27.405845899799665	27.53261810539167	22.701542345243237
60-61	22.66388311355055	27.747701369638495	27.154893783169527	22.43352173364143
62-63	22.322361495322827	27.861012693183113	27.45213707214714	22.364488739346925
64-65	22.35233961291868	27.432300260808056	27.380944418806337	22.83441570746693
66-67	22.393139793204636	27.673977289821533	27.20295863314114	22.729924283832695
68-69	22.81045512539834	27.48429675283318	27.189217399138755	22.516030722629722
70-71	22.847263223977272	27.503575162787236	26.975788205286182	22.673373407949306
72-73	22.544894070724823	27.682599660394498	27.31917734515371	22.45332892372697
74-75	22.74458278843012	27.365647719977016	27.05743082184286	22.832338669750012
76-77	22.75805993412314	27.66849657947068	27.089056609568267	22.48438687683791
78-79	22.67907694536509	27.369226950778526	27.327525745474684	22.624170358381704
80-81	22.825611717069958	27.358738400139487	27.114400294476624	22.701249588313928
82-83	22.423691349541745	27.470027871833935	27.47747892476563	22.62880185385869
84-85	22.56334438494339	27.258754459824956	27.071008759262977	23.10689239596867
86-87	22.715971999816993	27.41832517423861	27.230055969864726	22.63564685607967
88-89	22.562025530155346	27.09606257695655	27.420189974499205	22.921721918388894
90-91	22.763646810634818	27.181537605683943	27.137331735503878	22.917483848177362
92-93	22.39157744215899	27.388688286974787	27.420200802380567	22.799533468485656
94-95	22.404222850393467	26.979674958802036	27.354882285097386	23.261219905707105
96-97	22.501192356331373	27.120324259814826	27.367182504000237	23.011300879853565
98-99	22.425326168407516	26.983057503232217	27.41854416182521	23.173072166535057
100	22.635095901943398	26.815236166555767	27.188742742240535	23.3609251892603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	352.0
1	436.0
2	433.5
3	341.5
4	389.5
5	535.0
6	713.0
7	961.5
8	1385.5
9	2002.5
10	2783.5
11	3646.0
12	4614.0
13	5600.0
14	6531.0
15	7608.5
16	8655.0
17	9517.5
18	10509.0
19	11583.0
20	12632.5
21	13848.0
22	15172.5
23	16898.5
24	19399.5
25	22457.5
26	26524.5
27	32440.0
28	39805.0
29	47719.0
30	55929.5
31	64315.0
32	72847.5
33	81156.0
34	88957.0
35	96612.5
36	103492.0
37	108933.0
38	113476.0
39	116714.0
40	118198.5
41	118709.5
42	117670.0
43	114908.0
44	111468.0
45	107600.5
46	103400.5
47	99490.5
48	96408.0
49	94388.0
50	93127.5
51	89916.0
52	84069.0
53	78518.0
54	73791.5
55	69405.5
56	64653.5
57	59410.5
58	54518.5
59	50064.5
60	45162.5
61	39545.0
62	34068.0
63	28562.5
64	23667.0
65	19670.5
66	16033.0
67	12774.0
68	10031.0
69	7827.0
70	6047.5
71	4704.5
72	3649.5
73	2792.0
74	2171.0
75	1612.0
76	1159.5
77	837.5
78	567.0
79	378.5
80	252.5
81	177.5
82	131.0
83	97.0
84	71.0
85	52.0
86	37.5
87	26.0
88	19.5
89	10.5
90	7.0
91	5.5
92	5.5
93	3.5
94	1.0
95	1.0
96	1.0
97	1.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	7.545064283747068
2	24.795098786065562
3	5.97634873666797
4	23.15354838866573
5	12.397762182662266
6	9.532245836466915
7	7.895711194619219
8	8.215017232920136
9	4.768190017491308
10-11	6.580215306626816
12-13	9.698616928205386
14-15	7.005900960410793
16-17	7.571115814099684
18-19	6.462831427447552
20-21	7.6512007110821445
22-23	5.468541485162179
24-25	5.0380224669370515
26-27	9.574834160882336
28-29	6.167950598759619
30-31	3.9623708898923105
32-33	8.311714920261647
34-35	6.857100212364051
36-37	6.518810299261254
38-39	5.808503316782353
40-41	7.8067499302354
42-43	6.229857181680393
44-45	6.213198793543598
46-47	5.555101266584671
48-49	3.8474036929336215
50-51	6.428845883767009
52-53	4.194873958622752
54-55	4.72195387085615
56-57	4.619738852407289
58-59	3.59302889014401
60-61	3.926424641768659
62-63	3.0185576875725384
64-65	4.552998905045364
66-67	2.396224017626277
68-69	4.852469910026465
70-71	2.35717712061585
72-73	1.4659989530750228
74-75	0.68750809360555
76-77	1.0201438822677538
78-79	1.5903744915087819
80-81	1.9324794186830512
82-83	0.6578087410331971
84-85	1.1114762310944013
86-87	0.3369371790296428
88-89	0.6059032706981993
90-91	0.2208300376272862
92-93	0.25528675834454534
94-95	0.489735332258847
96-97	0.5077464544687951
98-99	0.4496548856194944
100	0.268570910928085
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3289634.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.97353954125302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.73062540261516	89.00415086178923
2	3.1510121217741216	5.859215001974677
3	0.635464590841845	1.7724417679110138
4	0.2019131547617511	0.7509032271256322
5	0.09028928502080281	0.4197257205516537
6	0.05124890468073096	0.28588752394679084
7	0.03373991225610734	0.2195843346383122
8	0.022254742229678832	0.16552817253371507
9	0.017402733767679403	0.14561943804677163
>10	0.06303137550776727	0.9450897955932545
>50	0.001585164653059085	0.0997227002756391
>100	0.0012682966060223659	0.1993745567318569
>500	9.858917417973976E-5	0.048153022470713236
>1k	6.57261111664538E-5	0.08460387641068798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.0797036995604985E-5	0.0
5	0.0	0.0	0.0	6.0797036995604985E-5	0.0
6	0.0	0.0	0.0	6.0797036995604985E-5	0.0
7	0.0	0.0	0.0	6.0797036995604985E-5	0.0
8	0.0	0.0	0.0	6.0797036995604985E-5	0.0
9	0.0	0.0	0.0	9.119555549340747E-5	0.0
10-11	0.0	0.0	0.0	9.119555549340747E-5	0.0
12-13	0.0	0.0	0.0	1.0639481474230872E-4	0.0
14-15	0.0	0.0	0.0	1.3679333324011122E-4	0.0
16-17	0.0	1.5199259248901246E-5	0.0	1.671918517379137E-4	3.0398518497802493E-5
18-19	0.0	3.0398518497802493E-5	0.0	1.975903702357162E-4	6.0797036995604985E-5
20-21	0.0	3.0398518497802493E-5	0.0	2.1278962948461744E-4	1.3679333324011122E-4
22-23	0.0	3.0398518497802493E-5	0.0	2.4318814798241994E-4	1.5199259248901246E-4
24-25	0.0	3.0398518497802493E-5	0.0	2.7358666648022244E-4	1.5199259248901246E-4
26-27	0.0	4.559777774670374E-5	0.0	2.887859257291237E-4	1.5199259248901246E-4
28-29	0.0	7.599629624450623E-5	0.0	6.535681477027536E-4	1.5199259248901246E-4
30-31	3.0398518497802493E-5	9.119555549340747E-5	0.0	0.0025534755538154093	1.5199259248901246E-4
32-33	3.0398518497802493E-5	9.119555549340747E-5	0.0	0.007994810364922056	1.5199259248901246E-4
34-35	4.559777774670374E-5	9.119555549340747E-5	0.0	0.016627989618297964	1.5199259248901246E-4
36-37	6.0797036995604985E-5	9.119555549340747E-5	0.0	0.030048935535077766	1.5199259248901246E-4
38-39	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.050446341447103235	1.5199259248901246E-4
40-41	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.07497794587482984	1.5199259248901246E-4
42-43	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.10090788215345536	1.5199259248901246E-4
44-45	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.13233995028018314	1.5199259248901246E-4
46-47	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.16980612432872472	1.8239111098681494E-4
48-49	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.20882262282065422	1.8239111098681494E-4
50-51	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.2510461650141019	1.8239111098681494E-4
52-53	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.29688713090878804	1.8239111098681494E-4
54-55	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.3482150293923275	1.8239111098681494E-4
56-57	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.40708176046332206	1.8239111098681494E-4
58-59	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.47183060486364137	1.8239111098681494E-4
60-61	9.119555549340747E-5	1.2159407399120997E-4	0.0	0.5430999314817393	1.8239111098681494E-4
62-63	9.119555549340747E-5	1.3679333324011122E-4	0.0	0.6161779699504565	1.8239111098681494E-4
64-65	9.119555549340747E-5	1.5199259248901246E-4	0.0	0.7023729691509755	2.1278962948461744E-4
66-67	9.119555549340747E-5	1.5199259248901246E-4	0.0	0.8043751979703517	2.1278962948461744E-4
68-69	9.119555549340747E-5	1.5199259248901246E-4	0.0	0.9208167230761841	2.1278962948461744E-4
70-71	9.119555549340747E-5	1.5199259248901246E-4	0.0	1.0511047733577656	2.2798888873351868E-4
72-73	9.119555549340747E-5	1.671918517379137E-4	0.0	1.1883084865975972	2.4318814798241994E-4
74-75	9.119555549340747E-5	1.8239111098681494E-4	0.0	1.335634906497197	2.4318814798241994E-4
76-77	9.119555549340747E-5	2.1278962948461744E-4	0.0	1.502765961198115	2.4318814798241994E-4
78-79	9.119555549340747E-5	2.1278962948461744E-4	0.0	1.6886073040344307	2.7358666648022244E-4
80-81	9.119555549340747E-5	2.1278962948461744E-4	0.0	1.8997554135201669	2.7358666648022244E-4
82-83	9.119555549340747E-5	2.1278962948461744E-4	0.0	2.13099694373295	2.7358666648022244E-4
84-85	9.119555549340747E-5	2.4318814798241994E-4	0.0	2.3711908376433364	2.7358666648022244E-4
86-87	9.119555549340747E-5	2.4318814798241994E-4	0.0	2.636828291536384	3.039851849780249E-4
88	1.2159407399120997E-4	2.4318814798241994E-4	0.0	2.852840163981768	3.039851849780249E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	215	1.4975338E-5	18.66693	5
GTACATG	9385	0.0	18.52479	1
TACATGG	9790	0.0	17.05682	2
GTATAAG	1070	0.0	16.768547	1
GTATCAA	6570	0.0	16.668217	1
CATGGGG	5030	0.0	16.209276	4
ACATGGG	10125	0.0	15.387896	3
GTACTAT	925	0.0	14.715064	1
GTATAGG	885	0.0	14.681054	1
GTACTAG	560	1.999797E-8	14.362716	1
TAGACTG	1250	0.0	14.046865	5
TATCAAC	7290	0.0	13.998239	2
TATACAG	1480	0.0	13.897718	5
TAGACCG	220	0.006913971	13.682012	5
ATCAACG	7205	0.0	13.408562	3
TCAACGC	7145	0.0	13.365112	4
GTCTAGA	1045	0.0	13.025297	1
ATAGGAC	815	3.6379788E-12	13.018653	3
GTCTAAG	815	1.05501385E-10	12.905432	1
GTATAAA	1870	0.0	12.903402	1
>>END_MODULE