FastQCFastQC Report
Wed 25 May 2016
SRR1295111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3289634
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50730.15421168433935203No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35860.10900908733311972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98350.022.636931
TCAACGC111900.019.6709984
CAACGCA111800.019.6465235
ATCAACG112050.019.6023923
AACGCAG113850.019.3444526
TATCAAC114400.019.051672
TATACCG1508.254835E-418.8135455
GAGTACT45050.018.44741812-13
GCTATAC6700.017.549683
ACGCAGA125400.017.4479967
CGCAGAG125900.017.3041158
GTACATG126850.016.1437781
CATGGGG60100.015.5736074
AGTACTT55750.015.45426512-13
TACATGG129700.015.2002092
GCAGAGT138650.015.0353549
ACATGGG126150.014.9134033
GTACTTT57400.014.5600214-15
GTCTAGG9350.014.3185071
ACGGTAC2308.611364E-414.3144363
Produced by FastQC (version 0.11.5)