FastQCFastQC Report
Wed 25 May 2016
SRR1295110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2644666
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG14700.016.848645
GTATTAC7550.016.4522861
GTACATG92200.016.4362261
GTCCTAA8350.015.619821
TACATGG95500.015.5413592
CATGGGG36900.015.52874854
CCTATAC7050.015.1404283
TATACTG12700.015.1239785
ACATGGG97800.015.10742953
GTATAAT11550.015.0563351
GTCCTAC5401.177068E-814.95177651
ACCGCGT2406.8469136E-414.7540158
CGTACAC2707.5495604E-414.5649143
GTGTTAC8350.014.1322181
GTACTGG11100.013.9881491
GTGCTAG8450.013.9649731
AGGACCG4401.9325671E-713.7852525
GTATAGG6801.1896191E-913.7001571
GTCTAAG8207.2759576E-1213.6333271
GTATCAA70650.013.3620981
Produced by FastQC (version 0.11.5)