##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295110_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2644666 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.402929897385906 27.0 22.0 33.0 6.0 33.0 2 21.531316241824108 27.0 2.0 33.0 2.0 33.0 3 23.796732744323858 27.0 15.0 33.0 6.0 33.0 4 23.757368983455756 33.0 6.0 37.0 2.0 37.0 5 25.6883640505077 33.0 15.0 37.0 2.0 37.0 6 26.49141214807465 33.0 15.0 37.0 2.0 37.0 7 26.18051428800461 33.0 15.0 37.0 6.0 37.0 8 26.828492898536147 33.0 15.0 37.0 6.0 37.0 9 27.83232438425117 33.0 15.0 37.0 6.0 37.0 10-11 27.43362375437957 33.0 15.0 37.0 6.0 37.0 12-13 26.2254802685859 33.0 15.0 37.0 4.0 37.0 14-15 27.79529721333431 33.0 15.0 37.0 4.0 40.0 16-17 28.243276844788717 33.0 15.0 37.0 6.0 40.0 18-19 28.41764650054109 33.0 15.0 37.0 6.0 40.0 20-21 27.912658914206936 33.0 15.0 37.0 6.0 40.0 22-23 28.68659917736304 33.0 22.0 37.0 6.0 40.0 24-25 28.785451546622525 33.0 22.0 37.0 6.0 40.0 26-27 27.383818221280116 33.0 15.0 37.0 4.0 40.0 28-29 28.088634443820126 33.0 15.0 37.0 6.0 40.0 30-31 28.847331572304405 33.0 22.0 37.0 6.0 40.0 32-33 27.59328720526524 33.0 15.0 37.0 6.0 40.0 34-35 27.142774550737222 33.0 15.0 37.0 6.0 40.0 36-37 27.39052417205046 33.0 15.0 37.0 6.0 40.0 38-39 27.520810189263976 33.0 18.5 37.0 6.0 40.0 40-41 26.695658733465777 33.0 15.0 37.0 6.0 40.0 42-43 27.241746783903903 33.0 15.0 37.0 6.0 40.0 44-45 27.054464533517653 33.0 15.0 37.0 6.0 40.0 46-47 26.827539091892888 33.0 15.0 37.0 6.0 40.0 48-49 26.683538299354247 33.0 15.0 37.0 6.0 40.0 50-51 26.541664240399356 33.0 15.0 37.0 6.0 40.0 52-53 26.81883137606034 33.0 15.0 37.0 6.0 40.0 54-55 26.755378183861403 33.0 15.0 37.0 6.0 40.0 56-57 26.719847421186646 33.0 15.0 37.0 6.0 40.0 58-59 26.554742262349954 30.0 15.0 37.0 6.0 40.0 60-61 26.54425076739369 30.0 15.0 37.0 6.0 40.0 62-63 26.500473405715503 27.0 15.0 37.0 6.0 38.5 64-65 26.059534928040062 27.0 15.0 37.0 6.0 37.0 66-67 26.301010978323916 27.0 18.5 37.0 6.0 37.0 68-69 25.857212971316606 27.0 18.5 37.0 6.0 37.0 70-71 26.001804008521304 27.0 15.0 37.0 6.0 37.0 72-73 26.023989796821226 27.0 18.5 33.0 6.0 37.0 74-75 26.1623220096602 27.0 22.0 33.0 6.0 37.0 76-77 25.920240589927047 27.0 22.0 33.0 6.0 37.0 78-79 25.51650794467052 27.0 15.0 33.0 6.0 37.0 80-81 25.354438518890476 27.0 15.0 33.0 6.0 37.0 82-83 25.459275575819404 27.0 15.0 33.0 6.0 37.0 84-85 25.40301875548746 27.0 18.5 33.0 6.0 37.0 86-87 25.223804631662375 27.0 18.5 33.0 6.0 37.0 88-89 25.17699569624293 27.0 18.5 33.0 6.0 37.0 90-91 25.305233061566184 27.0 22.0 33.0 6.0 37.0 92-93 25.237015940765296 27.0 22.0 33.0 6.0 37.0 94-95 25.118322691787924 27.0 15.0 33.0 6.0 37.0 96-97 25.201320507012984 27.0 22.0 33.0 6.0 37.0 98-99 25.275219819818457 27.0 18.5 33.0 6.0 37.0 100 24.935924612030405 27.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.257549011591099 1101 2 11.14647114059703 1101 3 8.294431731058872 1101 4 12.420722289719901 1101 5 10.601464185543154 1101 6 9.93695068365227 1101 7 9.516766049914033 1101 8 8.971142670008177 1101 9 7.267574136518153 1101 10-11 7.667453460200864 1101 12-13 9.094364866426211 1101 14-15 7.975748217989402 1101 16-17 8.156366348157533 1101 18-19 8.17102154151959 1101 20-21 7.175177656022274 1101 22-23 7.3434689654849095 1101 24-25 7.393641613004579 1101 26-27 8.351250540744335 1101 28-29 8.14685906904614 1101 30-31 5.51321514505954 1101 32-33 6.819581685890732 1101 34-35 8.580599669317468 1101 36-37 8.214562373671694 1101 38-39 8.094473383359489 1101 40-41 8.920445273770024 1101 42-43 8.489906930361183 1101 44-45 8.404143417392927 1101 46-47 7.194670736256555 1101 48-49 5.359320112776359 1101 50-51 6.665444458082252 1101 52-53 5.8428223673811495 1101 54-55 3.8944371802899624 1101 56-57 6.115060609483393 1101 58-59 6.251243092392144 1101 60-61 6.752878560482433 1101 62-63 6.287011848219194 1101 64-65 6.659364541266225 1101 66-67 5.765365156307833 1101 68-69 6.720900926504523 1101 70-71 6.322259117897733 1101 72-73 5.625350950458472 1101 74-75 4.993460879545271 1101 76-77 5.196741897615329 1101 78-79 5.131123736818527 1101 80-81 4.9431123530842065 1101 82-83 4.497062310284647 1101 84-85 4.264405213650708 1101 86-87 4.109642803847009 1101 88-89 4.085780869332979 1101 90-91 3.9107212207252076 1101 92-93 3.9530280120557926 1101 94-95 3.81229457720983 1101 96-97 3.9043661298822165 1101 98-99 3.5673418917276045 1101 100 3.7565368351713353 1102 1 6.622237150630561 1102 2 10.27022067212722 1102 3 8.041496039112847 1102 4 12.196735809198163 1102 5 10.290853083037266 1102 6 9.293972822223665 1102 7 6.692373764661657 1102 8 7.636402915081277 1102 9 6.3679329847028825 1102 10-11 7.243500807174808 1102 12-13 8.878348570876959 1102 14-15 6.029503508270391 1102 16-17 7.1344569873137615 1102 18-19 7.244532233274658 1102 20-21 6.939305346773224 1102 22-23 6.318129366353574 1102 24-25 6.9099892840287716 1102 26-27 8.257366499370985 1102 28-29 7.397491824154482 1102 30-31 4.100974957193287 1102 32-33 4.31684189665425 1102 34-35 4.923957640854791 1102 36-37 4.942690452847195 1102 38-39 4.501067954406434 1102 40-41 5.998129557238801 1102 42-43 6.6503555694790855 1102 44-45 6.285912813921168 1102 46-47 5.413770198610365 1102 48-49 3.5131380806548265 1102 50-51 4.83570723442622 1102 52-53 5.565042686993699 1102 54-55 5.2550726876779805 1102 56-57 6.211653761816564 1102 58-59 5.998910416408712 1102 60-61 5.949547068680484 1102 62-63 4.2699631159645435 1102 64-65 5.994671343441553 1102 66-67 3.79133650880787 1102 68-69 5.900093241358306 1102 70-71 5.214833027814041 1102 72-73 5.041765387430079 1102 74-75 4.182120322750826 1102 76-77 3.2918065830747167 1102 78-79 4.446993127248685 1102 80-81 4.432656945962815 1102 82-83 3.948815774143821 1102 84-85 3.1552792966050163 1102 86-87 3.252011607570779 1102 88-89 3.547943879420373 1102 90-91 3.4453729289980863 1102 92-93 3.2715198942707495 1102 94-95 3.235486315061575 1102 96-97 3.4379421889057156 1102 98-99 3.2528340219983356 1102 100 3.3586693886169634 1103 1 6.494561227209484 1103 2 10.728036037269103 1103 3 7.63785539752724 1103 4 11.416794262387821 1103 5 10.00034878995162 1103 6 8.70512940942207 1103 7 7.705789883772216 1103 8 7.878250113345423 1103 9 6.888584591572066 1103 10-11 7.14036778868233 1103 12-13 8.550771829198695 1103 14-15 8.322195672506606 1103 16-17 7.7662999184200885 1103 18-19 7.100003379353286 1103 20-21 8.086020603643 1103 22-23 6.531601745722021 1103 24-25 5.025493499018555 1103 26-27 5.705334288889517 1103 28-29 6.722752273849185 1103 30-31 5.870695067876987 1103 32-33 7.521504656861847 1103 34-35 6.626581880435399 1103 36-37 3.7981253624465516 1103 38-39 5.962494159753565 1103 40-41 6.386767359258922 1103 42-43 7.174366141784297 1103 44-45 5.488285584831022 1103 46-47 6.081118475534538 1103 48-49 5.447191686902997 1103 50-51 6.152862395906592 1103 52-53 5.893361308108194 1103 54-55 4.704234312996533 1103 56-57 5.78083323047732 1103 58-59 5.247821041631678 1103 60-61 4.890185967791254 1103 62-63 2.4783765704443397 1103 64-65 4.681146233748109 1103 66-67 3.7717482239366618 1103 68-69 4.822761033438386 1103 70-71 3.443032911982261 1103 72-73 4.241570460162315 1103 74-75 3.128972653474925 1103 76-77 3.3616060079143217 1103 78-79 2.871285375703483 1103 80-81 3.9870959459109585 1103 82-83 3.2552521850580014 1103 84-85 3.1504918329274005 1103 86-87 2.6583192852685364 1103 88-89 2.8687484604610702 1103 90-91 2.574539639919074 1103 92-93 2.393882331536343 1103 94-95 2.778968497022433 1103 96-97 2.844291819699727 1103 98-99 2.711998696179279 1103 100 2.7303127816759307 1104 1 6.11974870413216 1104 2 9.465919214608558 1104 3 7.122015057953941 1104 4 11.739750384384799 1104 5 9.167497235187994 1104 6 7.794861553115954 1104 7 3.955971347313632 1104 8 6.2373627444449475 1104 9 6.404193205108143 1104 10-11 6.840905712969331 1104 12-13 7.1383023568705575 1104 14-15 6.9399194343279795 1104 16-17 6.862505334274303 1104 18-19 6.610156122361051 1104 20-21 7.2977305156320895 1104 22-23 6.226767830626592 1104 24-25 5.733487328820811 1104 26-27 7.881966570469448 1104 28-29 6.918377000123197 1104 30-31 5.968287434975018 1104 32-33 7.213127001524494 1104 34-35 7.636364324111099 1104 36-37 6.250724580113459 1104 38-39 6.921082174100707 1104 40-41 6.772214164419747 1104 42-43 6.96781024420358 1104 44-45 6.272848469804568 1104 46-47 6.40852668634588 1104 48-49 5.154179673260586 1104 50-51 5.336329346050821 1104 52-53 5.599880937971303 1104 54-55 4.604166182167848 1104 56-57 5.663395674365443 1104 58-59 3.616489513458127 1104 60-61 3.923773872804194 1104 62-63 3.4853026111653946 1104 64-65 2.6473553107362555 1104 66-67 2.9354886595366274 1104 68-69 3.8049101627944957 1104 70-71 2.1707519755566658 1104 72-73 2.2803007589195907 1104 74-75 3.087648228900843 1104 76-77 3.747992150085029 1104 78-79 4.161443891557251 1104 80-81 2.820054741910205 1104 82-83 2.760138205711545 1104 84-85 2.7402419699075438 1104 86-87 0.6048377718082492 1104 88-89 0.15139215528244065 1104 90-91 1.9916758084861748 1104 92-93 2.3983417925999326 1104 94-95 2.2523722020150068 1104 96-97 2.748020397224238 1104 98-99 2.399652365403952 1104 100 0.7008307821470048 1105 1 5.46547738837759 1105 2 -2.6641516781643713 1105 3 0.5177847493848091 1105 4 7.6729323968501255 1105 5 7.951175025093146 1105 6 7.447049698802452 1105 7 4.843117856730771 1105 8 5.574393475920001 1105 9 4.415601329641774 1105 10-11 4.53017930213349 1105 12-13 4.595014978843114 1105 14-15 5.364510129110471 1105 16-17 5.623988986736794 1105 18-19 5.736370421533167 1105 20-21 6.521458564105245 1105 22-23 1.8973838444909177 1105 24-25 3.639671103856866 1105 26-27 6.4541613325791936 1105 28-29 5.40533997730417 1105 30-31 4.9782093250888835 1105 32-33 6.702592054303814 1105 34-35 5.715345463077316 1105 36-37 5.724815156335399 1105 38-39 3.888703628626022 1105 40-41 4.868256223317395 1105 42-43 5.137001827830542 1105 44-45 5.708370239073748 1105 46-47 4.744767529452098 1105 48-49 4.587968685454971 1105 50-51 5.568681428356449 1105 52-53 5.292548299217284 1105 54-55 4.900578315297402 1105 56-57 4.742252163855074 1105 58-59 1.533528393368215 1105 60-61 4.020594178547814 1105 62-63 -0.8847144542344694 1105 64-65 -0.6063327804969418 1105 66-67 0.44691076020746934 1105 68-69 2.412450796757124 1105 70-71 2.574700735945857 1105 72-73 2.24142129051506 1105 74-75 2.376866279482723 1105 76-77 1.5055902786581719 1105 78-79 2.9086816011062417 1105 80-81 1.5179595264940744 1105 82-83 0.6639298844140917 1105 84-85 1.0448062608327966 1105 86-87 -0.6728776654568165 1105 88-89 1.2040247736811445 1105 90-91 1.2664249920336808 1105 92-93 1.494291066351888 1105 94-95 0.8844682465347127 1105 96-97 1.7876965685475774 1105 98-99 1.6126282844248863 1105 100 1.8449502482749658 1106 1 5.946555196947944 1106 2 10.064223556606901 1106 3 6.327941417066086 1106 4 11.211425713696652 1106 5 9.450164525000922 1106 6 9.104421599018302 1106 7 6.10220780319419 1106 8 7.028532648760997 1106 9 6.639630054032001 1106 10-11 5.372976640915869 1106 12-13 7.531329246332703 1106 14-15 7.5815798129438114 1106 16-17 7.528538286616779 1106 18-19 6.896344667855072 1106 20-21 7.829698317855701 1106 22-23 5.854941638770683 1106 24-25 4.810684597180039 1106 26-27 7.263928570287529 1106 28-29 5.778705497358434 1106 30-31 5.023089666696741 1106 32-33 7.071554355787196 1106 34-35 6.299993237219191 1106 36-37 5.640773397600945 1106 38-39 6.779619463677992 1106 40-41 6.335172703318289 1106 42-43 5.859578879490467 1106 44-45 4.044872367860609 1106 46-47 5.585105162360534 1106 48-49 5.380134276658566 1106 50-51 6.127223794466293 1106 52-53 5.178377223038165 1106 54-55 4.906268743359792 1106 56-57 4.522784384607249 1106 58-59 4.7852493430245 1106 60-61 4.6223929382925775 1106 62-63 4.6767108891964 1106 64-65 4.760859896536985 1106 66-67 2.774005908010583 1106 68-69 3.196718452075576 1106 70-71 2.7359947048764752 1106 72-73 2.5164166033127593 1106 74-75 2.5462333376478057 1106 76-77 3.256983819959064 1106 78-79 3.8805698364435237 1106 80-81 3.3239985723441485 1106 82-83 1.8087066205070208 1106 84-85 2.454029099918671 1106 86-87 3.1720839146654995 1106 88-89 3.1447668343448 1106 90-91 2.98231825766889 1106 92-93 2.7684171909325386 1106 94-95 1.2153041423318172 1106 96-97 1.276252362448318 1106 98-99 1.7441888811582444 1106 100 2.6893917450321325 1107 1 5.805549227850385 1107 2 9.974069902349541 1107 3 5.615434208256314 1107 4 10.587741067649212 1107 5 8.621369949681043 1107 6 7.393413010381419 1107 7 3.197739635673635 1107 8 5.687587294585068 1107 9 5.214540121591746 1107 10-11 4.111813075560395 1107 12-13 5.572058831186489 1107 14-15 5.627164810395346 1107 16-17 6.483521427139841 1107 18-19 5.822252952503558 1107 20-21 5.121118197011686 1107 22-23 5.289345594579665 1107 24-25 4.154596795399971 1107 26-27 6.639924794800322 1107 28-29 5.850835731106631 1107 30-31 4.452484388121931 1107 32-33 4.32994811366866 1107 34-35 6.003578592065235 1107 36-37 5.936616160768192 1107 38-39 5.8755249993331695 1107 40-41 7.048010781266843 1107 42-43 5.497852671608701 1107 44-45 3.6532282667991858 1107 46-47 3.0556774809371277 1107 48-49 3.4587631635777214 1107 50-51 4.3704791787364705 1107 52-53 3.3976934572777786 1107 54-55 4.75777062526527 1107 56-57 4.457515719374218 1107 58-59 2.474685504952646 1107 60-61 2.3061444206675468 1107 62-63 0.14499726627197873 1107 64-65 1.5458325868241793 1107 66-67 3.674748783472811 1107 68-69 4.352723568299865 1107 70-71 3.712563915542262 1107 72-73 1.9855284398738107 1107 74-75 2.0408278725577382 1107 76-77 2.7102633715101767 1107 78-79 3.5407981348801236 1107 80-81 1.9806460087492468 1107 82-83 1.1702249405294367 1107 84-85 1.8192093810825618 1107 86-87 1.8453222744683657 1107 88-89 2.1568381639945358 1107 90-91 0.9100703730991917 1107 92-93 1.8930715361187787 1107 94-95 1.9668199595773217 1107 96-97 1.9848358465574627 1107 98-99 1.590845757550511 1107 100 -0.1776790718783161 1108 1 4.060675472075211 1108 2 -3.1386828644218756 1108 3 0.03149697993631406 1108 4 5.93375992774445 1108 5 6.450555909106789 1108 6 6.259020346437243 1108 7 3.6892025581534327 1108 8 4.436370838683885 1108 9 4.480860467419198 1108 10-11 3.044712764599069 1108 12-13 2.106829791345991 1108 14-15 3.4601808885379732 1108 16-17 4.189743247060598 1108 18-19 3.4522601509166186 1108 20-21 4.1745052780741645 1108 22-23 3.639845150529929 1108 24-25 2.4635441128841435 1108 26-27 4.2087201485978945 1108 28-29 5.600885829025291 1108 30-31 4.083243023687405 1108 32-33 4.142624879620289 1108 34-35 2.685652044975722 1108 36-37 0.973728692585432 1108 38-39 2.0178920564093765 1108 40-41 4.815029524972974 1108 42-43 2.54216418578034 1108 44-45 3.077042932332322 1108 46-47 4.192619129512877 1108 48-49 4.342897905472235 1108 50-51 1.8474813559717695 1108 52-53 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-2.9072781785898307 2212 84-85 -5.3063042546153625 2212 86-87 -4.867020318815065 2212 88-89 -3.3721687816588712 2212 90-91 -4.316102560821076 2212 92-93 -5.4153545245199375 2212 94-95 -3.764029667095535 2212 96-97 -5.525906462378348 2212 98-99 -3.953654627244493 2212 100 -3.989625885886234 2213 1 -20.525825416024258 2213 2 -16.511507020727382 2213 3 -19.282179751857647 2213 4 -18.191817336909192 2213 5 -20.42741922410813 2213 6 -21.15163684716844 2213 7 -10.075251020214132 2213 8 -9.737715733380416 2213 9 -4.516508738292853 2213 10-11 -16.935596916005736 2213 12-13 -15.641734340977623 2213 14-15 -22.667083765103232 2213 16-17 -17.170845415403896 2213 18-19 -17.854506718164053 2213 20-21 -16.84982383812259 2213 22-23 -18.863068665348134 2213 24-25 -7.379233506368056 2213 26-27 -22.337372213746686 2213 28-29 -23.20044632017542 2213 30-31 -18.609429033561344 2213 32-33 -22.393609578639033 2213 34-35 -17.081872530051047 2213 36-37 -22.745987115585084 2213 38-39 -10.810885732767993 2213 40-41 -16.21245827568236 2213 42-43 -22.25401698651096 2213 44-45 -16.269519529594817 2213 46-47 -16.06867905068475 2213 48-49 -5.880143145883643 2213 50-51 -22.3422166902094 2213 52-53 -16.85063712476219 2213 54-55 -16.722047545098928 2213 56-57 -16.56342882277971 2213 58-59 -17.075350428995744 2213 60-61 -18.2337117278303 2213 62-63 -16.551607393441884 2213 64-65 -16.72435117128828 2213 66-67 -8.252220769737718 2213 68-69 -21.64709836902207 2213 70-71 -16.42685337595084 2213 72-73 -8.811530568940615 2213 74-75 -4.155404269748413 2213 76-77 -2.942542047766363 2213 78-79 -4.51472045820616 2213 80-81 -3.331448368327081 2213 82-83 -3.2472796492450087 2213 84-85 -3.0420167958024606 2213 86-87 -3.2786054925099464 2213 88-89 -2.7696765901375464 2213 90-91 -2.8524030139229346 2213 92-93 -3.698154730761818 2213 94-95 -3.808112993717547 2213 96-97 -3.48783378224935 2213 98-99 -3.7156216460513747 2213 100 -3.1771326701586275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 764.0 3 872.0 4 5117.0 5 16253.0 6 21535.0 7 25472.0 8 24817.0 9 26092.0 10 30791.0 11 38768.0 12 50716.0 13 64439.0 14 59600.0 15 64103.0 16 67650.0 17 68097.0 18 66048.0 19 64753.0 20 64050.0 21 62840.0 22 64688.0 23 68607.0 24 72716.0 25 78022.0 26 83090.0 27 87761.0 28 91918.0 29 95619.0 30 99748.0 31 108246.0 32 120842.0 33 141615.0 34 163209.0 35 179914.0 36 182204.0 37 143375.0 38 40253.0 39 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.207447521790705 20.9005931198102 13.945969364072411 23.94598999432668 2 15.147134122466191 21.243642344854834 40.00257292638214 23.606650606296828 3 17.94469671040429 27.086036543699976 29.64790209323949 25.321364652656243 4 12.913851786588937 18.03944759557584 38.603018982335485 30.443681635499736 5 13.328381869062511 38.24739172793265 35.21020672728052 13.214019675724314 6 30.767399365761882 38.073189843901645 18.25568025399972 12.903730536336752 7 26.376905218699765 32.66292965509005 23.361560638396238 17.59860448781394 8 24.665588710862853 36.16562661101835 21.53449144482512 17.634293233293675 9 24.777866500741812 17.53677718218092 22.034737644681755 35.65061867239551 10-11 23.53566363414477 27.689162128672812 28.735267169108138 20.039907068074285 12-13 24.403472718071352 25.290622112212972 28.563626004857053 21.742279164858623 14-15 21.655416310554735 26.823432134181125 26.21704793045152 25.304103624812623 16-17 21.259015204757567 30.201875344276967 27.134930145325143 21.40417930564032 18-19 21.37683554848533 29.269727092324388 28.541661369084974 20.811775990105303 20-21 22.671580849141826 28.570685249709637 27.675767195767197 21.08196670538134 22-23 21.59043769878711 28.642021256264123 28.166742778281833 21.600798266666935 24-25 21.86588407723967 28.507064943352546 27.72799852994251 21.899052449465273 26-27 21.977686089592456 28.397924385314923 27.744758336140112 21.87963118895251 28-29 21.769062893067744 28.278837055294716 28.24930094841695 21.702799103220592 30-31 22.177411480759183 28.674302727351446 27.68280754132824 21.465478250561127 32-33 21.524681396200045 28.532966758726253 28.12915931853725 21.81319252653645 34-35 21.563986566509843 28.20689406976516 28.05304363757713 22.176075726147868 36-37 22.076441518208323 28.57653824006262 27.63562967561308 21.711390566115977 38-39 21.915982101601113 28.392079754431414 27.801130337996234 21.89080780597124 40-41 22.003821998194745 28.408750566074993 27.493136501731776 22.09429093399849 42-43 21.810991115452456 28.497437286292598 27.906567708285785 21.785003889969154 44-45 22.274746660698174 28.083464367985112 27.7956281884478 21.846160782868914 46-47 22.15607421192527 28.36260339202902 27.920157827176666 21.561164568869042 48-49 22.081186723940277 28.216717606505043 27.88609865293889 21.815997016615793 50-51 22.197015892606654 28.19150668358969 27.736347672039646 21.875129751764014 52-53 21.781435420499594 28.318954510434907 27.699379751682756 22.20023031738274 54-55 22.190768707309125 28.367447429122766 27.452588768266423 21.989195095301685 56-57 22.2421932720678 28.140004882944957 27.50671803865186 22.111083806335383 58-59 22.11269463718919 27.79293044649397 27.83177912370931 22.262595792607527 60-61 22.385218728025116 28.21192492757553 27.48028856738885 21.922567777010503 62-63 21.97434357266992 28.318923281500407 27.69732303959215 22.009410106237524 64-65 21.94659200515799 27.94865623395706 27.799414042042613 22.30533771884234 66-67 22.079580033471817 28.12348064134164 27.52884946799484 22.268089857191704 68-69 22.569371843244234 27.9338382608553 27.459610833778697 22.037179062121773 70-71 22.50326388181108 28.019258116024325 27.329266221128602 22.148211781035997 72-73 22.304583441611964 28.138741808413094 27.576705991926183 21.979968758048766 74-75 22.482070179717393 27.88955916650153 27.338441077389753 22.289929576391323 76-77 22.45502159158769 28.124743486886906 27.442698438544287 21.977536482981115 78-79 22.48994421980604 27.94837824250897 27.535018336359713 22.026659201325277 80-81 22.53140480656115 27.849126366865463 27.454525052094287 22.164943774479102 82-83 22.10154699411817 28.057806951952895 27.672159156862186 22.168486897066746 84-85 22.242023795727956 27.83207567589311 27.345453709618567 22.580446818760375 86-87 22.47122184062113 27.904166920634882 27.440701032031896 22.183910206712092 88-89 22.366039050409178 27.590920599024216 27.675002522267338 22.36803782829927 90-91 22.48185767678879 27.704354574831797 27.468621016531507 22.34516673184791 92-93 22.171335757060557 27.93814941595258 27.684265253922113 22.206249573064753 94-95 22.02035285711543 27.622397228754185 27.67461279788963 22.682637116240752 96-97 22.280245891382886 27.676187230702116 27.638762693060603 22.404804184854395 98-99 22.23150118715052 27.59304662471005 27.61985814956426 22.555594038575176 100 22.431796445842394 27.37998572075062 27.446138390168525 22.742079443238463 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 333.0 1 384.0 2 363.0 3 304.5 4 351.5 5 459.5 6 669.0 7 1022.0 8 1498.5 9 2100.0 10 2870.0 11 3702.0 12 4544.0 13 5404.0 14 6218.0 15 7099.0 16 7973.0 17 8682.5 18 9564.5 19 10666.0 20 11673.0 21 12742.5 22 14058.5 23 15844.5 24 17901.5 25 20485.5 26 24330.5 27 29246.0 28 35305.0 29 42077.5 30 48798.0 31 55814.5 32 63033.5 33 70083.5 34 76243.5 35 81575.0 36 86331.0 37 90232.5 38 93482.5 39 95222.0 40 95972.5 41 95607.0 42 94041.5 43 91879.5 44 88820.5 45 85770.0 46 82722.5 47 79951.5 48 77490.0 49 75464.0 50 74794.5 51 71884.0 52 65670.5 53 59813.5 54 55260.5 55 51262.0 56 47240.0 57 43182.0 58 38944.0 59 34861.0 60 30953.5 61 26838.0 62 22976.0 63 19155.0 64 15774.0 65 13016.0 66 10410.5 67 8314.5 68 6583.5 69 5135.0 70 3958.5 71 3064.0 72 2401.0 73 1861.5 74 1423.5 75 1039.5 76 749.0 77 543.0 78 388.0 79 273.0 80 191.5 81 139.0 82 102.0 83 67.0 84 45.5 85 32.5 86 20.0 87 16.0 88 13.0 89 9.5 90 5.0 91 3.0 92 2.0 93 1.5 94 2.5 95 1.0 96 0.0 97 0.5 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.357993032012361 2 25.931705553744784 3 6.4274656988822025 4 24.42323529700915 5 13.605498766195806 6 10.585041740620554 7 8.807728461741483 8 9.710602397429392 9 5.624793452178838 10-11 7.12602271893691 12-13 10.901130804419159 14-15 8.220678906145427 16-17 8.429192949128547 18-19 7.062649877148948 20-21 8.435980195608822 22-23 6.022726499300857 24-25 5.550965603974188 26-27 10.189037103362013 28-29 6.673829511930807 30-31 4.152036589875621 32-33 9.090977839923832 34-35 7.5409900531863 36-37 7.44613875627395 38-39 6.424989015626171 40-41 8.279741184709147 42-43 6.805906681599868 44-45 6.913878727975479 46-47 6.018245782265133 48-49 4.183174737377045 50-51 7.110614346008154 52-53 4.5660775311513815 54-55 5.31704192514291 56-57 5.217369603571869 58-59 3.9825822996174187 60-61 4.340396859187512 62-63 3.277219127103385 64-65 4.8760977756737525 66-67 2.747095474437982 68-69 5.511452107751981 70-71 2.6138839460256986 72-73 1.711993121248581 74-75 0.7768844912741344 76-77 1.190906526570841 78-79 1.8065230165170194 80-81 2.1118167662759686 82-83 0.8098376127647121 84-85 1.2978387440984986 86-87 0.4334195698057902 88-89 0.6829217753773066 90-91 0.31864137097085227 92-93 0.3632216695794478 94-95 0.5378561980983609 96-97 0.5813588559008964 98-99 0.4337031594915956 100 0.48682896063245795 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2644666.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.47059983344728 #Duplication Level Percentage of deduplicated Percentage of total 1 97.5844510259757 93.16446073867559 2 1.8150550306904938 3.46568785021475 3 0.3036196524645238 0.8696025102603268 4 0.11082934731496301 0.4232377706923591 5 0.055832205209621906 0.26651670606933614 6 0.031227590559340674 0.17887900812321297 7 0.021119652504779204 0.14114141250336662 8 0.013638259800117217 0.10416422750412652 9 0.009530196954196702 0.08188682577732463 >10 0.052304165668627646 0.7886682512343007 >50 9.546171361094649E-4 0.06024426707434237 >100 0.0012390749396292012 0.2468678812967654 >500 1.1950846917664389E-4 0.07598757128150499 >1k 7.967231278343627E-5 0.13265497929267892 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.1343587432212612E-4 0.0 5 0.0 0.0 0.0 1.1343587432212612E-4 0.0 6 0.0 0.0 0.0 1.1343587432212612E-4 0.0 7 3.781195810737537E-5 0.0 0.0 1.1343587432212612E-4 0.0 8 3.781195810737537E-5 0.0 0.0 1.1343587432212612E-4 0.0 9 3.781195810737537E-5 0.0 0.0 1.1343587432212612E-4 0.0 10-11 3.781195810737537E-5 0.0 0.0 1.1343587432212612E-4 0.0 12-13 3.781195810737537E-5 0.0 0.0 1.512478324295015E-4 0.0 14-15 3.781195810737537E-5 0.0 0.0 1.8905979053687687E-4 0.0 16-17 3.781195810737537E-5 0.0 0.0 2.4577772769793997E-4 0.0 18-19 3.781195810737537E-5 0.0 0.0 3.2140164391269066E-4 0.0 20-21 3.781195810737537E-5 0.0 0.0 3.403076229663784E-4 0.0 22-23 3.781195810737537E-5 0.0 0.0 3.403076229663784E-4 0.0 24-25 3.781195810737537E-5 0.0 0.0 3.7811958107375373E-4 0.0 26-27 3.781195810737537E-5 0.0 0.0 4.348375182348168E-4 0.0 28-29 3.781195810737537E-5 0.0 0.0 7.562391621475075E-4 0.0 30-31 3.781195810737537E-5 0.0 0.0 0.0017771620310466428 0.0 32-33 3.781195810737537E-5 0.0 0.0 0.005615075778945243 0.0 34-35 3.781195810737537E-5 0.0 0.0 0.010398288479528228 0.0 36-37 3.781195810737537E-5 1.8905979053687687E-5 0.0 0.01879254317936556 0.0 38-39 3.781195810737537E-5 3.781195810737537E-5 0.0 0.03257500190950389 0.0 40-41 3.781195810737537E-5 3.781195810737537E-5 0.0 0.047813221026776154 0.0 42-43 3.781195810737537E-5 3.781195810737537E-5 0.0 0.06452610651023608 0.0 44-45 3.781195810737537E-5 7.562391621475075E-5 0.0 0.08381020514499751 0.0 46-47 3.781195810737537E-5 7.562391621475075E-5 0.0 0.10681878165333543 0.0 48-49 3.781195810737537E-5 7.562391621475075E-5 0.0 0.13103734082110935 0.0 50-51 3.781195810737537E-5 7.562391621475075E-5 0.0 0.1574300875800574 0.0 52-53 3.781195810737537E-5 1.1343587432212612E-4 0.0 0.1866965431551659 0.0 54-55 3.781195810737537E-5 1.1343587432212612E-4 0.0 0.22004669020587098 0.0 56-57 5.671793716106306E-5 1.1343587432212612E-4 0.0 0.25899300705646766 0.0 58-59 7.562391621475075E-5 1.1343587432212612E-4 0.0 0.3029683143353452 0.0 60-61 7.562391621475075E-5 1.1343587432212612E-4 0.0 0.3502332619695644 0.0 62-63 7.562391621475075E-5 1.1343587432212612E-4 0.0 0.4005987901685884 0.0 64-65 7.562391621475075E-5 1.3234185337581382E-4 0.0 0.4576759409316715 0.0 66-67 7.562391621475075E-5 1.7015381148318917E-4 0.0 0.5264937046870948 0.0 68-69 7.562391621475075E-5 1.8905979053687687E-4 0.0 0.6054450732152945 0.0 70-71 7.562391621475075E-5 1.8905979053687687E-4 0.0 0.7007312076458805 0.0 72-73 7.562391621475075E-5 1.8905979053687687E-4 0.0 0.7973407606102245 0.0 74-75 7.562391621475075E-5 1.8905979053687687E-4 0.0 0.9013614573636142 0.0 76-77 7.562391621475075E-5 1.8905979053687687E-4 0.0 1.0130390756337473 0.0 78-79 7.562391621475075E-5 1.8905979053687687E-4 0.0 1.146288416004138 0.0 80-81 7.562391621475075E-5 1.8905979053687687E-4 0.0 1.2975551544126933 0.0 82-83 7.562391621475075E-5 1.8905979053687687E-4 0.0 1.457556455144052 0.0 84-85 7.562391621475075E-5 1.8905979053687687E-4 0.0 1.6269540274650938 0.0 86-87 7.562391621475075E-5 2.2687174864425224E-4 0.0 1.809585785123717 0.0 88 7.562391621475075E-5 2.2687174864425224E-4 0.0 1.9614953268200974 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACAG 1470 0.0 16.84864 5 GTATTAC 755 0.0 16.452286 1 GTACATG 9220 0.0 16.436226 1 GTCCTAA 835 0.0 15.61982 1 TACATGG 9550 0.0 15.541359 2 CATGGGG 3690 0.0 15.5287485 4 CCTATAC 705 0.0 15.140428 3 TATACTG 1270 0.0 15.123978 5 ACATGGG 9780 0.0 15.1074295 3 GTATAAT 1155 0.0 15.056335 1 GTCCTAC 540 1.177068E-8 14.9517765 1 ACCGCGT 240 6.8469136E-4 14.754015 8 CGTACAC 270 7.5495604E-4 14.564914 3 GTGTTAC 835 0.0 14.132218 1 GTACTGG 1110 0.0 13.988149 1 GTGCTAG 845 0.0 13.964973 1 AGGACCG 440 1.9325671E-7 13.785252 5 GTATAGG 680 1.1896191E-9 13.700157 1 GTCTAAG 820 7.2759576E-12 13.633327 1 GTATCAA 7065 0.0 13.362098 1 >>END_MODULE