FastQCFastQC Report
Wed 25 May 2016
SRR1295110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2644666
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58780.22225868975515242No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA44490.16822540161971306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATA1303.18179E-421.671438
CCGATAC1300.00808901418.05859
CCTATAC8150.017.88923
GAGTACT37400.017.82673312-13
CCCCTAT8450.017.6726511
GTATCAA108250.017.3820531
CAACGCA116900.015.8919685
TCAACGC117700.015.8638694
ATCAACG118350.015.855923
TATACTG15800.015.7766165
AACGCAG117600.015.653076
CTAGGAC9450.015.4282513
TATCAAC119600.015.4253812
AGTACTT44400.015.06908712-13
TAGGGCG1900.004011166314.8522655
CATGGGG41750.014.7574014
CTATACA12900.014.5836214
GTACTTT46000.014.5441214-15
TAGGGGT6800.014.5246434
ACGCAGA130400.014.1509777
Produced by FastQC (version 0.11.5)