##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295110_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2644666 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19562470270348 33.0 33.0 33.0 27.0 33.0 2 29.883130799881723 33.0 33.0 33.0 22.0 33.0 3 30.734933258112743 33.0 33.0 33.0 27.0 33.0 4 34.974171785775596 37.0 37.0 37.0 33.0 37.0 5 35.32483345723052 37.0 37.0 37.0 33.0 37.0 6 35.07264168707882 37.0 37.0 37.0 33.0 37.0 7 34.95129781983812 37.0 37.0 37.0 33.0 37.0 8 34.9424796930879 37.0 37.0 37.0 33.0 37.0 9 34.88414453847858 37.0 37.0 37.0 33.0 37.0 10-11 34.86786459991545 37.0 37.0 37.0 33.0 37.0 12-13 34.77565824947271 37.0 37.0 37.0 30.0 37.0 14-15 36.19646072509723 37.0 37.0 40.0 27.0 40.0 16-17 36.188050211255415 37.0 37.0 40.0 27.0 40.0 18-19 36.20243841755443 38.5 37.0 40.0 27.0 40.0 20-21 36.19761833819469 38.5 37.0 40.0 27.0 40.0 22-23 36.16059135633762 37.0 37.0 40.0 27.0 40.0 24-25 36.13520932321889 37.0 37.0 40.0 27.0 40.0 26-27 36.114624871344816 37.0 37.0 40.0 27.0 40.0 28-29 36.0509434839787 37.0 37.0 40.0 27.0 40.0 30-31 35.95190337834721 37.0 37.0 40.0 27.0 40.0 32-33 35.91175823336482 37.0 37.0 40.0 27.0 40.0 34-35 35.81636925040818 37.0 37.0 40.0 27.0 40.0 36-37 35.80491865513453 37.0 37.0 40.0 27.0 40.0 38-39 35.73456912895617 37.0 37.0 40.0 27.0 40.0 40-41 35.66335314175778 37.0 37.0 40.0 27.0 40.0 42-43 35.56814111876509 37.0 37.0 40.0 27.0 40.0 44-45 35.45935384657269 37.0 37.0 40.0 27.0 40.0 46-47 35.3508737965399 37.0 35.0 40.0 27.0 40.0 48-49 35.23456496964078 37.0 33.0 40.0 27.0 40.0 50-51 35.11963646827236 37.0 33.0 40.0 27.0 40.0 52-53 35.02234043920858 37.0 33.0 40.0 27.0 40.0 54-55 34.89685219229952 37.0 33.0 40.0 27.0 40.0 56-57 34.76685657092427 37.0 33.0 40.0 27.0 40.0 58-59 34.63118178250108 37.0 33.0 40.0 27.0 40.0 60-61 34.463964636744294 37.0 33.0 40.0 22.0 40.0 62-63 34.27612314749764 37.0 33.0 40.0 22.0 40.0 64-65 34.09443158417736 37.0 33.0 40.0 22.0 40.0 66-67 33.87110716438295 37.0 33.0 38.5 22.0 40.0 68-69 33.65694231332047 37.0 33.0 37.0 22.0 40.0 70-71 33.430248469939116 37.0 33.0 37.0 22.0 40.0 72-73 33.21370543577147 37.0 33.0 37.0 22.0 40.0 74-75 32.97891359438205 37.0 33.0 37.0 22.0 40.0 76-77 32.745332302831436 37.0 33.0 37.0 22.0 37.0 78-79 32.523830230358016 37.0 33.0 37.0 22.0 37.0 80-81 32.3174935889825 37.0 33.0 37.0 22.0 37.0 82-83 32.10954767066994 33.0 33.0 37.0 22.0 37.0 84-85 31.936491035162852 33.0 33.0 37.0 22.0 37.0 86-87 31.973115697785655 33.0 33.0 37.0 22.0 37.0 88-89 31.990236196177513 33.0 33.0 37.0 22.0 37.0 90-91 32.006538065676345 33.0 33.0 37.0 22.0 37.0 92-93 31.98126096074136 33.0 33.0 37.0 22.0 37.0 94-95 32.0066388345447 33.0 33.0 37.0 22.0 37.0 96-97 31.970867398756592 33.0 33.0 37.0 22.0 37.0 98-99 31.89341508530756 33.0 33.0 37.0 22.0 37.0 100 31.356131171195152 33.0 27.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7163048114525523 1101 2 4.118932194350247 1101 3 2.3758374651989307 1101 4 1.2883156629517032 1101 5 0.7060079906430232 1101 6 0.7211660408632454 1101 7 0.7386676643958197 1101 8 0.8214951399436998 1101 9 0.8565921967621009 1101 10-11 0.8505672335729955 1101 12-13 0.8474527659831566 1101 14-15 1.1592593903526378 1101 16-17 1.1446637787985878 1101 18-19 1.215558512512402 1101 20-21 1.1585444141433712 1101 22-23 1.119454647061282 1101 24-25 1.1181330810182288 1101 26-27 1.1377891024672167 1101 28-29 1.1025255514560683 1101 30-31 1.0383518261204685 1101 32-33 1.1785811100573653 1101 34-35 1.3079943317564968 1101 36-37 1.1154369431375315 1101 38-39 1.1902640265221365 1101 40-41 1.1569333928351426 1101 42-43 1.2390632434514686 1101 44-45 1.2858351700816257 1101 46-47 1.2889709978572697 1101 48-49 1.2897206574966091 1101 50-51 1.2798969136860663 1101 52-53 1.3090373777648097 1101 54-55 1.2351741454263774 1101 56-57 1.2545753368578048 1101 58-59 1.2477599819117913 1101 60-61 1.2408933043799166 1101 62-63 1.2981484656873121 1101 64-65 1.1760226911662883 1101 66-67 1.1572816356802562 1101 68-69 1.2065050772104087 1101 70-71 1.1455482198004177 1101 72-73 1.0806516066607443 1101 74-75 1.0970697392668498 1101 76-77 1.02788263760079 1101 78-79 1.2364089382512446 1101 80-81 1.1077933080514413 1101 82-83 1.1202979292347202 1101 84-85 1.1094487686256116 1101 86-87 1.1506615642551665 1101 88-89 1.0888269295532425 1101 90-91 1.0962416627574179 1101 92-93 1.1948546740241213 1101 94-95 1.1449392203163207 1101 96-97 0.9933211845759722 1101 98-99 1.1183481820374723 1101 100 1.2307891551321255 1102 1 0.6127274363935875 1102 2 4.023974601119903 1102 3 2.311763129605879 1102 4 1.0969043157208205 1102 5 0.5578479421988973 1102 6 0.5537474225051398 1102 7 0.5601421324939793 1102 8 0.47120826049167164 1102 9 0.5932485551104136 1102 10-11 0.6723994792466428 1102 12-13 0.6883384440738709 1102 14-15 1.0020673442155186 1102 16-17 0.9950578443927469 1102 18-19 0.9481326252809907 1102 20-21 0.9296544180539072 1102 22-23 0.9908133077954844 1102 24-25 0.9593850514714148 1102 26-27 0.8849925271145409 1102 28-29 0.950181862597411 1102 30-31 0.9438611687826679 1102 32-33 1.0829797112782131 1102 34-35 1.0556990957923347 1102 36-37 0.9587985459810753 1102 38-39 0.9586743733907639 1102 40-41 0.9800001261007978 1102 42-43 0.9272739699042987 1102 44-45 1.0080793436194995 1102 46-47 0.9957378556015968 1102 48-49 0.984235362846789 1102 50-51 0.8331907463381896 1102 52-53 1.033592376840076 1102 54-55 0.8552518628924659 1102 56-57 0.7945021434983843 1102 58-59 0.8333824259887379 1102 60-61 0.8389588574710558 1102 62-63 0.9175808749951315 1102 64-65 0.8760822132838868 1102 66-67 0.9195586750437386 1102 68-69 0.9345424363392141 1102 70-71 0.9441174100203824 1102 72-73 0.817774306118686 1102 74-75 0.8556930897929576 1102 76-77 0.7418327512168545 1102 78-79 0.7212136121680857 1102 80-81 0.81895833501639 1102 82-83 0.8847290531853496 1102 84-85 0.8905478698522842 1102 86-87 0.9376434128579945 1102 88-89 0.8406848435531415 1102 90-91 0.8442498912973058 1102 92-93 0.9138032180393694 1102 94-95 0.8896062714840589 1102 96-97 0.9542879610040949 1102 98-99 0.9431812640385075 1102 100 0.9935919825923598 1103 1 0.6397491664105779 1103 2 4.01807636439948 1103 3 2.314980991441182 1103 4 1.196692582223882 1103 5 0.5521705605719163 1103 6 0.6197694130499158 1103 7 0.6632878337541968 1103 8 0.5888081310399684 1103 9 0.6479226548369539 1103 10-11 0.6631279386902165 1103 12-13 0.719583785655793 1103 14-15 0.9053738604716344 1103 16-17 0.9076629536113643 1103 18-19 0.8531901756824993 1103 20-21 0.8737019828063168 1103 22-23 0.8853084867818168 1103 24-25 0.8906942442288681 1103 26-27 0.9417528796580044 1103 28-29 0.9034753524639996 1103 30-31 0.964123264738113 1103 32-33 1.0561674343590397 1103 34-35 1.0845285887877552 1103 36-37 0.9852455529415991 1103 38-39 0.8791956226496325 1103 40-41 0.8867059989719763 1103 42-43 0.8781247462083925 1103 44-45 0.9094591100441463 1103 46-47 0.9109148801743103 1103 48-49 0.8946480713549292 1103 50-51 0.8740635581702776 1103 52-53 0.9440307632671363 1103 54-55 0.8585461649560102 1103 56-57 0.7927072194794107 1103 58-59 0.9085530654438898 1103 60-61 0.9681793608207485 1103 62-63 1.0572024037122318 1103 64-65 0.9161388921355851 1103 66-67 0.9235623999038935 1103 68-69 0.9029047723925672 1103 70-71 0.861916331297877 1103 72-73 0.9017661541603914 1103 74-75 0.8481168541758706 1103 76-77 0.7970180976844503 1103 78-79 0.8234302538336635 1103 80-81 0.8210075391570939 1103 82-83 0.8983624349797701 1103 84-85 0.8353593995502635 1103 86-87 0.8720732224852767 1103 88-89 0.7734847925721695 1103 90-91 0.8267150589816161 1103 92-93 0.8465119198550042 1103 94-95 0.7960614254047478 1103 96-97 0.8313001320877582 1103 98-99 0.8751545527551556 1103 100 0.9098673473468857 1104 1 0.4036979305279651 1104 2 3.5552579285283628 1104 3 2.1542800154928337 1104 4 1.0931716459732215 1104 5 0.3989428586581951 1104 6 0.3121903659818557 1104 7 0.29921074251886637 1104 8 0.2954172899975376 1104 9 0.36164528458073164 1104 10-11 0.3848061625029544 1104 12-13 0.434250281969355 1104 14-15 0.4921135582219165 1104 16-17 0.5552249258004949 1104 18-19 0.48394848024010884 1104 20-21 0.6030458151388771 1104 22-23 0.5788865392210027 1104 24-25 0.583718503302201 1104 26-27 0.5986434336200475 1104 28-29 0.5955782365753635 1104 30-31 0.4049347557005518 1104 32-33 0.6605659181747683 1104 34-35 0.6956742113273506 1104 36-37 0.5953964841253949 1104 38-39 0.579897267098552 1104 40-41 0.5497655011452309 1104 42-43 0.5738256940422275 1104 44-45 0.719863915251203 1104 46-47 0.6763279729140734 1104 48-49 0.5670650820078293 1104 50-51 0.5286226695518437 1104 52-53 0.5545664258980167 1104 54-55 0.6076514363016443 1104 56-57 0.571430689534651 1104 58-59 0.5441005205497049 1104 60-61 0.47902284609529744 1104 62-63 0.6329203701959827 1104 64-65 0.5797793337957913 1104 66-67 0.5677101147877792 1104 68-69 0.6625019102105227 1104 70-71 0.681141938993008 1104 72-73 0.533469293676454 1104 74-75 0.5628562607847769 1104 76-77 0.516006231487026 1104 78-79 0.5464891187446952 1104 80-81 0.5238996787775037 1104 82-83 0.5842491385035196 1104 84-85 0.7000928396354311 1104 86-87 0.6765342767044231 1104 88-89 0.6483634784624925 1104 90-91 0.662148931467943 1104 92-93 0.6793560086543735 1104 94-95 0.5856603063223069 1104 96-97 0.4925922624616099 1104 98-99 0.5439633472237162 1104 100 0.48897058195247567 1105 1 0.4222933230592929 1105 2 3.635472627235025 1105 3 2.124624986941761 1105 4 0.9843687112746551 1105 5 0.36863281303983086 1105 6 0.26597818941585416 1105 7 0.2654114580213758 1105 8 0.21475510250932928 1105 9 0.2611376132272909 1105 10-11 0.22989071403174677 1105 12-13 0.2717114027533398 1105 14-15 0.4718670885689349 1105 16-17 0.4196834006461643 1105 18-19 0.4692938220724443 1105 20-21 0.3571178242360489 1105 22-23 0.40221456730098737 1105 24-25 0.49142599278287236 1105 26-27 0.4770334040663542 1105 28-29 0.39767121202368116 1105 30-31 0.41046552017569127 1105 32-33 0.5249822437668996 1105 34-35 0.5804603625465106 1105 36-37 0.39322531802591953 1105 38-39 0.4407534004648497 1105 40-41 0.3167980586672883 1105 42-43 0.49457386600764863 1105 44-45 0.5492878737702895 1105 46-47 0.4935049231783353 1105 48-49 0.4626642834940071 1105 50-51 0.4960316741719808 1105 52-53 0.3967645107318418 1105 54-55 0.4314908711180081 1105 56-57 0.393660975641005 1105 58-59 0.3776831212873688 1105 60-61 0.327372166489063 1105 62-63 0.40658616649318446 1105 64-65 0.31897186767388774 1105 66-67 0.23011248216244695 1105 68-69 0.2714585193207455 1105 70-71 0.32422647689269724 1105 72-73 0.3614136745815344 1105 74-75 0.36327853573142477 1105 76-77 0.24225440149641742 1105 78-79 0.3758797616861216 1105 80-81 0.2869686290251039 1105 82-83 0.44933843245430793 1105 84-85 0.3063931308564847 1105 86-87 0.38580203942834856 1105 88-89 0.29266460282538276 1105 90-91 0.3396844583039531 1105 92-93 0.3921847523251998 1105 94-95 0.3175377993907098 1105 96-97 0.28082548437705057 1105 98-99 0.31548397997739386 1105 100 0.17271886459123564 1106 1 0.45976684131509415 1106 2 3.862746021561449 1106 3 2.2039741983152297 1106 4 0.9561425181507843 1106 5 0.3126263751896019 1106 6 0.2124717193055332 1106 7 0.1705122474196159 1106 8 0.1909605611201286 1106 9 0.258198275619435 1106 10-11 0.21802317323031417 1106 12-13 0.2575845798364753 1106 14-15 0.3135444326951031 1106 16-17 0.23945009815601281 1106 18-19 0.35500382632066163 1106 20-21 0.3401452496197095 1106 22-23 0.23413819765745814 1106 24-25 0.2581809066281693 1106 26-27 0.3960221018995682 1106 28-29 0.3631770085937873 1106 30-31 0.2906206836508858 1106 32-33 0.3503890182860232 1106 34-35 0.4728393827554953 1106 36-37 0.3070512617729122 1106 38-39 0.3171146500413542 1106 40-41 0.32991384382364686 1106 42-43 0.4557543110249256 1106 44-45 0.4665530601159915 1106 46-47 0.34162890998990036 1106 48-49 0.32656965665982796 1106 50-51 0.23905672495948949 1106 52-53 0.26660722821107186 1106 54-55 0.23033142426101705 1106 56-57 0.2557625666077712 1106 58-59 0.20347638441766946 1106 60-61 0.3567758531476315 1106 62-63 0.36646760494433295 1106 64-65 0.36264305657358165 1106 66-67 0.18908731700972936 1106 68-69 0.21197042633271934 1106 70-71 0.16564086960207192 1106 72-73 0.31933126382371313 1106 74-75 0.24331538055732693 1106 76-77 0.2912300675156061 1106 78-79 0.29867157703388614 1106 80-81 0.3145307701947786 1106 82-83 0.24841113178438334 1106 84-85 0.34744356641839147 1106 86-87 0.3740083489466528 1106 88-89 0.3265999475188224 1106 90-91 0.2912853308193881 1106 92-93 0.4144085749012447 1106 94-95 0.3845829465739179 1106 96-97 0.40497457688155336 1106 98-99 0.4521408813229826 1106 100 0.40136078282972676 1107 1 0.43931793553118936 1107 2 3.470960917196159 1107 3 2.031656357830961 1107 4 0.9158202691239765 1107 5 0.1951872490191846 1107 6 0.16701573162752226 1107 7 0.09809454969008868 1107 8 0.13864440099926156 1107 9 0.1975294148347544 1107 10-11 0.13834374189371346 1107 12-13 0.10753732913438085 1107 14-15 0.039983174057226734 1107 16-17 0.060041733296827715 1107 18-19 0.15655005255950982 1107 20-21 0.17802317908708432 1107 22-23 0.14020017209968927 1107 24-25 0.25231626066195645 1107 26-27 0.12963767028883666 1107 28-29 0.08022215046518966 1107 30-31 0.08941652051247218 1107 32-33 0.1065858660055028 1107 34-35 0.3305377316334628 1107 36-37 0.17673381269654698 1107 38-39 0.12575654073955178 1107 40-41 0.10121194232239361 1107 42-43 0.1020719545360862 1107 44-45 0.2442864174109971 1107 46-47 0.10473009680367085 1107 48-49 0.039953725799421136 1107 50-51 0.012489671860009821 1107 52-53 0.01382479262839098 1107 54-55 0.10755987296576563 1107 56-57 0.0582884548651279 1107 58-59 0.04959723249860559 1107 60-61 0.08750751688000236 1107 62-63 0.16155883700099594 1107 64-65 -0.003333810761979805 1107 66-67 0.13188503070016822 1107 68-69 0.13727008690946718 1107 70-71 0.10191778333799562 1107 72-73 0.04447832100480298 1107 74-75 0.09143997315803887 1107 76-77 0.0972735503454416 1107 78-79 0.022876475775490235 1107 80-81 0.15550347469653047 1107 82-83 0.13463796459888755 1107 84-85 0.1610757810662946 1107 86-87 0.1915328132102445 1107 88-89 0.1258847269800114 1107 90-91 0.059154104590692924 1107 92-93 0.13264190122883335 1107 94-95 0.07555573991663778 1107 96-97 0.050995491567828566 1107 98-99 0.16755058793457778 1107 100 0.07621287531368282 1108 1 0.40056884007694293 1108 2 3.5667969704922484 1108 3 2.083491700141437 1108 4 1.0155521314002414 1108 5 0.23306658894115628 1108 6 0.10283340303578115 1108 7 0.09508772574631763 1108 8 0.1005282345527263 1108 9 0.1873770589674777 1108 10-11 0.14570370809027366 1108 12-13 0.1361380221555848 1108 14-15 0.08224956088400148 1108 16-17 0.008453970852045245 1108 18-19 0.11102979959032666 1108 20-21 0.12373335503705363 1108 22-23 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0.3766116448894863 2210 74-75 -0.17420515890412958 2210 76-77 0.1730315653480048 2210 78-79 0.2816540202464779 2210 80-81 0.2760326329189837 2210 82-83 0.2225406958954146 2210 84-85 0.33308051813619244 2210 86-87 0.35587514021548117 2210 88-89 0.3774682413893373 2210 90-91 0.2999491715929672 2210 92-93 0.5029697561474791 2210 94-95 0.3668815902533815 2210 96-97 0.31415186779802085 2210 98-99 0.2849569818983042 2210 100 0.2712516355513337 2211 1 0.4195725095607834 2211 2 3.832888636009386 2211 3 2.1124670177549127 2211 4 1.0279189224055756 2211 5 0.46612365626464225 2211 6 0.2866471917813982 2211 7 0.26207744540837297 2211 8 0.23293350749813868 2211 9 0.16258450734180485 2211 10-11 0.19731428014768682 2211 12-13 0.1682728833897471 2211 14-15 0.08224960828722772 2211 16-17 0.08569430663754929 2211 18-19 0.22713978645488453 2211 20-21 0.16327890503536935 2211 22-23 0.05258292730670888 2211 24-25 0.07195616061610366 2211 26-27 0.1182609050589889 2211 28-29 0.11560636952135184 2211 30-31 0.08580430025193664 2211 32-33 0.11765005924043237 2211 34-35 0.22632387621864325 2211 36-37 0.09031859790363939 2211 38-39 -0.04806285665871002 2211 40-41 0.03498616968148127 2211 42-43 0.15864413535766886 2211 44-45 0.19481920831609045 2211 46-47 0.28153186585424095 2211 48-49 0.2025305108742046 2211 50-51 0.14878873960423533 2211 52-53 0.15073353614742047 2211 54-55 0.19013825761080483 2211 56-57 0.22842406477755617 2211 58-59 0.20772088241569264 2211 60-61 0.2585286642822595 2211 62-63 0.33458543491750703 2211 64-65 0.2655421874724979 2211 66-67 0.1949316218192152 2211 68-69 0.08522923770588875 2211 70-71 0.2402945816427433 2211 72-73 0.23134398162446956 2211 74-75 0.24503122222566986 2211 76-77 0.10945316583269715 2211 78-79 0.1654492234323044 2211 80-81 0.25254442654633635 2211 82-83 -0.157416904367512 2211 84-85 0.021173545887837264 2211 86-87 0.21074875576486107 2211 88-89 0.16394352159404946 2211 90-91 0.14649099689655642 2211 92-93 0.28837824243855437 2211 94-95 0.41370745787219576 2211 96-97 0.25610901258406926 2211 98-99 0.27341113106994186 2211 100 0.2708003116445141 2212 1 0.6183198035485766 2212 2 4.2028610445861005 2212 3 2.4212780497683646 2212 4 1.2765370862544643 2212 5 0.6579671407515519 2212 6 0.5864939233535296 2212 7 0.5292848097479492 2212 8 0.4660358639387354 2212 9 0.28547078920919944 2212 10-11 0.3529512371753114 2212 12-13 0.48127854171530515 2212 14-15 0.4130959860036967 2212 16-17 0.6396305691296007 2212 18-19 0.4922730699326223 2212 20-21 0.5504138959249403 2212 22-23 0.5097962893315966 2212 24-25 0.4055225287445623 2212 26-27 0.4606603338163566 2212 28-29 0.4809528982800799 2212 30-31 0.447405317243998 2212 32-33 0.4213985219614642 2212 34-35 0.5609283849145115 2212 36-37 0.6203294801949824 2212 38-39 0.41839417576321836 2212 40-41 0.4619543203416825 2212 42-43 0.5936549956462827 2212 44-45 0.5094243356769468 2212 46-47 0.5186728880803599 2212 48-49 0.5338308566794652 2212 50-51 0.4796028250788851 2212 52-53 0.4935545760378517 2212 54-55 0.4626136579116391 2212 56-57 0.4739816488985298 2212 58-59 0.3072440832589365 2212 60-61 0.4660103948979142 2212 62-63 0.5798677907730934 2212 64-65 0.7264413950377175 2212 66-67 0.5812480464429157 2212 68-69 0.5865736045022629 2212 70-71 0.6209544279808483 2212 72-73 0.526066277404837 2212 74-75 0.514608086432851 2212 76-77 0.6760859741809 2212 78-79 0.5572524170379509 2212 80-81 0.4714639527266087 2212 82-83 0.5878727641600712 2212 84-85 0.6930137662050555 2212 86-87 0.43938915272516255 2212 88-89 0.6836815234407254 2212 90-91 0.6199505922482409 2212 92-93 0.519381862017049 2212 94-95 0.5845846057592787 2212 96-97 0.5392165849659314 2212 98-99 0.6834407389787032 2212 100 0.8005851482431794 2213 1 -0.8159300922800128 2213 2 -25.703540507305267 2213 3 -14.687403805498155 2213 4 -6.452775259895741 2213 5 -2.1612569485802453 2213 6 -1.5251537531863661 2213 7 -1.415487711410222 2213 8 -1.3069571294398088 2213 9 -1.2746728797953395 2213 10-11 -1.192737773528954 2213 12-13 -1.2578077524787545 2213 14-15 -1.3858219006648227 2213 16-17 -1.5388833338141552 2213 18-19 -1.4257190830715416 2213 20-21 -1.52292408864718 2213 22-23 -1.484723331659552 2213 24-25 -1.3816935822828995 2213 26-27 -1.6803733091715998 2213 28-29 -1.5500737678496819 2213 30-31 -1.6569286948083715 2213 32-33 -1.580554473097834 2213 34-35 -1.5427311391534602 2213 36-37 -1.621417999557245 2213 38-39 -1.6980462609513367 2213 40-41 -1.5755432285663602 2213 42-43 -1.6469908058082652 2213 44-45 -1.3209938031038817 2213 46-47 -1.4412001103404393 2213 48-49 -1.5393272352504965 2213 50-51 -1.5614468375458088 2213 52-53 -1.6663312468462408 2213 54-55 -1.5037424787472347 2213 56-57 -1.6294336413632848 2213 58-59 -1.6002987583269928 2213 60-61 -1.5790910102780842 2213 62-63 -1.6201176777531359 2213 64-65 -1.5121999821958454 2213 66-67 -1.6314966688277401 2213 68-69 -1.7151400589347965 2213 70-71 -1.4899747722278427 2213 72-73 -1.7596697680033913 2213 74-75 -1.6217511928760864 2213 76-77 -1.577420400408979 2213 78-79 -1.6606560049140313 2213 80-81 -1.6732397627786568 2213 82-83 -1.631940506401964 2213 84-85 -1.6089559488029792 2213 86-87 -1.4637918394068272 2213 88-89 -1.551576909191013 2213 90-91 -1.4975357166523757 2213 92-93 -1.515874907373714 2213 94-95 -1.6114563554889578 2213 96-97 -1.7152352108090874 2213 98-99 -1.595483021556614 2213 100 -1.8603627415417172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 25.0 5 35.0 6 33.0 7 46.0 8 54.0 9 87.0 10 447.0 11 2157.0 12 6057.0 13 10993.0 14 14285.0 15 15957.0 16 16809.0 17 17198.0 18 17628.0 19 18346.0 20 20083.0 21 21881.0 22 24430.0 23 26163.0 24 27142.0 25 28514.0 26 31221.0 27 35371.0 28 41229.0 29 48230.0 30 58708.0 31 71613.0 32 89496.0 33 119876.0 34 162494.0 35 229973.0 36 348253.0 37 580883.0 38 555295.0 39 3642.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.1656137012079 20.740179057500608 10.618176882947754 30.476030358343742 2 13.034294229624319 22.357281971089545 36.24422388669002 28.364199912596117 3 15.364964800848197 26.889747136311353 28.70975011589365 29.0355379469468 4 10.699946077130123 18.63825468384031 37.17974315168208 33.48205608734748 5 11.79468107145116 38.381900751358025 35.05318663439401 14.770231542796806 6 29.912628235297678 39.4049912454203 17.626025844548327 13.056354674733692 7 26.10059681461829 33.06197143248369 22.987575755512534 17.849855997385482 8 24.594113563845383 36.76906112459264 20.59870589948686 18.03811941207512 9 24.743521023880792 16.9324354126152 21.669139945732866 36.65490361777115 10-11 23.203892805244173 27.735253836375307 28.847246130052334 20.21360722832819 12-13 24.00984270398219 25.062505142169766 29.1248712684397 21.802780885408342 14-15 21.572457016575257 26.684809150227405 26.21371361431486 25.52902021888248 16-17 20.959639754477774 30.04587229704747 27.239236653204113 21.755251295270643 18-19 21.027920291281653 29.044898230890976 29.140438855229668 20.786742622597703 20-21 22.0612423890462 28.347335206469165 28.484624540146918 21.106797864337715 22-23 21.316031278643425 28.254465088564263 28.590409261885362 21.83909437090695 24-25 21.44644388160505 28.173696255067348 28.465663414056706 21.914196449270896 26-27 21.409361823785243 28.045801489189394 28.535775393678048 22.00906129334732 28-29 21.178483904151037 28.0625226915164 28.948021299770062 21.810972104562506 30-31 21.721028823059203 28.412376421206943 28.368787352097375 21.497807403636482 32-33 20.93141881365725 28.29760435478358 28.917199065881377 21.853777765677794 34-35 21.073681559625847 28.01477586447466 28.952560762887757 21.95898181301173 36-37 21.65001392481432 28.263761885486034 28.43528383460459 21.650940355095056 38-39 21.451396355882387 28.359258440651637 28.553131895775717 21.63621330769026 40-41 21.49060189363151 28.15813072852648 28.45833921819871 21.892928159643304 42-43 21.363597861509362 28.19578654723307 28.56801445424851 21.872601137009063 44-45 21.671256767452245 27.89553553297669 28.475011335021644 21.958196364549423 46-47 21.602841811893335 28.295644646444135 28.475886255669657 21.62562728599287 48-49 21.546579555453686 28.25539485695126 28.400006580996468 21.798019006598583 50-51 21.652878996720798 28.16937550714528 28.13660430178328 22.041141194350644 52-53 21.334167698749454 28.340738042732294 28.102701834828554 22.222392423689698 54-55 21.641406763394652 28.370119278936844 27.918324894454926 22.07014906321358 56-57 21.6816711994468 28.07367590852098 28.0089885379879 22.23566435404432 58-59 21.398068966143185 27.822702789376173 28.457903333342156 22.321324911138483 60-61 21.806576968471255 28.21395738610902 27.937367571375795 22.042098074043935 62-63 21.24024983123344 28.413329627087364 28.199520835342458 22.146899706336733 64-65 21.227279319872043 27.984764099004234 28.360404735057532 22.427551846066194 66-67 21.49949028381292 28.09475067021391 28.109197829068556 22.29656121690461 68-69 21.72028260059264 28.055157003165913 28.05746416290666 22.16709623333479 70-71 21.64972979678782 28.013650859930046 27.896798560933394 22.43982078234874 72-73 21.46760291813334 28.11681761742257 28.19635562376821 22.21922384067588 74-75 21.769350575869023 27.84587315299069 27.9340727169853 22.450703554154984 76-77 21.544727284829374 28.140220991926007 28.111837783439714 22.203213939804904 78-79 21.59926936356726 28.102595423311538 28.195418773423285 22.102716439697915 80-81 21.603005990766246 27.947870898353997 28.02110335003013 22.428019760849622 82-83 21.25255629135066 28.17006597954362 28.090957081532082 22.48642064757364 84-85 21.32213953324796 27.834156241887275 28.010341174426294 22.833363050438468 86-87 21.43770649449165 28.007525976189296 27.98298392741604 22.57178360190301 88-89 21.268041593818367 27.76217399302963 28.26083544984939 22.708948963302607 90-91 21.392259907675523 27.837597408183655 28.10725370134554 22.66288898279528 92-93 21.024407822543015 28.118985298888816 28.176745336278746 22.679861542289423 94-95 20.925981734313783 27.725607088474725 28.37386387741806 22.97454729979343 96-97 21.197113185869334 27.807583284791853 28.196274321076302 22.799029208262507 98-99 21.099381355044578 27.760316956755354 28.164358926754417 22.97594276144565 100 21.16402076345402 27.597579943953964 28.18116882059932 23.057230471992693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34.0 1 27.0 2 20.0 3 21.5 4 20.5 5 31.0 6 57.5 7 121.0 8 204.5 9 241.5 10 220.0 11 210.0 12 252.0 13 323.0 14 405.0 15 473.0 16 557.0 17 681.0 18 855.0 19 1066.0 20 1396.5 21 1934.5 22 2699.5 23 4044.5 24 5952.0 25 8616.0 26 12753.0 27 18403.0 28 25889.5 29 34310.5 30 42513.0 31 51276.0 32 60016.5 33 68059.0 34 76011.5 35 83474.5 36 89811.5 37 95681.0 38 100363.5 39 102880.5 40 104773.5 41 104879.5 42 102664.5 43 100557.5 44 97737.0 45 93728.5 46 90533.5 47 87880.5 48 84648.5 49 83242.0 50 89649.5 51 86614.5 52 72293.0 53 64869.0 54 60915.5 55 57357.5 56 53180.0 57 48442.5 58 44031.5 59 39996.0 60 35442.0 61 30352.5 62 25575.0 63 20848.5 64 16662.5 65 13576.0 66 10704.5 67 8051.0 68 6082.0 69 4678.5 70 3510.5 71 2602.5 72 2045.0 73 1532.5 74 1105.0 75 778.5 76 470.0 77 316.5 78 218.5 79 113.5 80 50.0 81 21.5 82 13.5 83 12.5 84 9.5 85 5.0 86 2.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8166626712030934 2 5.431044978836647 3 0.0 4 0.0055961697998915556 5 0.16058738608202322 6 0.055886074082700805 7 0.03645072761550986 8 0.04276532461944155 9 0.04072347888164328 10-11 0.042443922975528854 12-13 0.04096925660934122 14-15 0.04337031594915955 16-17 0.0345412237310874 18-19 0.028907241973088475 20-21 0.023254354236035855 22-23 0.023651379796163295 24-25 0.020153773671231074 26-27 0.01291278369366869 28-29 0.01913285080233194 30-31 0.02474792658127718 32-33 0.0012856065756507626 34-35 0.003138392522912156 36-37 0.004026973538435477 38-39 0.005955383401911621 40-41 0.006446938857307501 42-43 0.00695740029175707 44-45 0.007827075328226702 46-47 0.016542731671976726 48-49 0.02607134511503532 50-51 0.0217985938489019 52-53 0.027999754978511465 54-55 0.02471011462316981 56-57 0.015483996844970215 58-59 0.014481979955124768 60-61 0.011986390720037994 62-63 0.018187551849647553 64-65 0.01952987636245938 66-67 0.020702047063788018 68-69 0.027319139732578705 70-71 0.027602729418384025 72-73 0.024464336895471867 74-75 0.0250315162670825 76-77 0.01879254317936556 78-79 0.014973535410520648 80-81 0.013101843484205566 82-83 0.007940511202548827 84-85 0.010795314039655668 86-87 0.00850769057415946 88-89 0.007051930187025508 90-91 0.007127554103240258 92-93 0.003932443643167039 94-95 0.0013234185337581382 96-97 7.751451412011951E-4 98-99 5.671793716106306E-4 100 0.10999498613435496 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2644666.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.70744317662219 #Duplication Level Percentage of deduplicated Percentage of total 1 90.08300377525757 74.50534915921558 2 7.281533910369525 12.044741042610703 3 1.3593193372502776 3.3727748053353324 4 0.4769492429801474 1.5778900964765408 5 0.2203515956323828 0.9112358537321668 6 0.14100298694933985 0.6997197918507917 7 0.08338511542848925 0.48275987872545 8 0.06002932050733208 0.3971897291833128 9 0.04516692164742074 0.33620765450552986 >10 0.23318866767210886 3.35808586694543 >50 0.011692099565889106 0.657767886520586 >100 0.0035049841908367987 0.569173582200546 >500 3.671758100542417E-4 0.1887002315200104 >1k 4.589697623541792E-4 0.6752742742356533 >5k 4.589697623541792E-5 0.22313014694233352 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5878 0.22225868975515242 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4449 0.16822540161971306 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 3.781195810737537E-5 0.0 0.0 0.0 4 0.0 3.781195810737537E-5 0.0 0.0 0.0 5 0.0 3.781195810737537E-5 0.0 0.0 0.0 6 0.0 3.781195810737537E-5 0.0 0.0 0.0 7 0.0 3.781195810737537E-5 0.0 3.781195810737537E-5 0.0 8 0.0 3.781195810737537E-5 0.0 3.781195810737537E-5 0.0 9 0.0 3.781195810737537E-5 0.0 3.781195810737537E-5 0.0 10-11 0.0 3.781195810737537E-5 0.0 3.781195810737537E-5 0.0 12-13 0.0 3.781195810737537E-5 0.0 3.781195810737537E-5 0.0 14-15 0.0 3.781195810737537E-5 0.0 7.562391621475075E-5 0.0 16-17 0.0 3.781195810737537E-5 0.0 9.452989526843843E-5 0.0 18-19 1.8905979053687687E-5 3.781195810737537E-5 0.0 1.1343587432212612E-4 0.0 20-21 3.781195810737537E-5 3.781195810737537E-5 0.0 1.512478324295015E-4 0.0 22-23 3.781195810737537E-5 3.781195810737537E-5 0.0 1.512478324295015E-4 0.0 24-25 3.781195810737537E-5 3.781195810737537E-5 0.0 1.8905979053687687E-4 0.0 26-27 3.781195810737537E-5 3.781195810737537E-5 0.0 2.835896858053153E-4 0.0 28-29 3.781195810737537E-5 3.781195810737537E-5 0.0 6.617092668790691E-4 0.0 30-31 3.781195810737537E-5 3.781195810737537E-5 0.0 0.0024955892350867746 0.0 32-33 3.781195810737537E-5 7.562391621475075E-5 0.0 0.00850769057415946 0.0 34-35 3.781195810737537E-5 9.452989526843843E-5 0.0 0.016637261567245164 0.0 36-37 3.781195810737537E-5 1.1343587432212612E-4 0.0 0.030173942569685547 0.0 38-39 3.781195810737537E-5 1.1343587432212612E-4 0.0 0.05136754508886944 0.0 40-41 3.781195810737537E-5 1.1343587432212612E-4 0.0 0.07685280485324045 0.0 42-43 3.781195810737537E-5 1.1343587432212612E-4 0.0 0.10254603038720202 0.0 44-45 3.781195810737537E-5 1.1343587432212612E-4 0.0 0.13287122078931707 0.0 46-47 3.781195810737537E-5 1.512478324295015E-4 0.0 0.16945429025820274 0.0 48-49 3.781195810737537E-5 1.512478324295015E-4 0.0 0.20892997452230264 0.0 50-51 3.781195810737537E-5 1.512478324295015E-4 0.0 0.249464393613409 0.0 52-53 3.781195810737537E-5 1.512478324295015E-4 0.0 0.2942148460334878 0.0 54-55 3.781195810737537E-5 1.512478324295015E-4 0.0 0.34601722864059203 0.0 56-57 5.671793716106306E-5 1.512478324295015E-4 0.0 0.4063651137799631 0.0 58-59 7.562391621475075E-5 1.512478324295015E-4 0.0 0.47419976662459457 0.0 60-61 7.562391621475075E-5 1.512478324295015E-4 0.0 0.5466663843373795 0.0 62-63 7.562391621475075E-5 1.512478324295015E-4 0.0 0.6229331038399556 0.0 64-65 7.562391621475075E-5 1.512478324295015E-4 0.0 0.7084448470997851 0.0 66-67 7.562391621475075E-5 1.512478324295015E-4 0.0 0.8085520061890613 0.0 68-69 7.562391621475075E-5 1.512478324295015E-4 0.0 0.9223092821551001 0.0 70-71 7.562391621475075E-5 1.8905979053687687E-4 0.0 1.0551615969653634 0.0 72-73 7.562391621475075E-5 1.8905979053687687E-4 0.0 1.1883731253776468 3.781195810737537E-5 74-75 7.562391621475075E-5 2.2687174864425224E-4 0.0 1.328882361704654 3.781195810737537E-5 76-77 7.562391621475075E-5 2.2687174864425224E-4 0.0 1.4840059198401612 3.781195810737537E-5 78-79 7.562391621475075E-5 2.2687174864425224E-4 0.0 1.6664108057501401 3.781195810737537E-5 80-81 7.562391621475075E-5 2.2687174864425224E-4 0.0 1.8674380810280011 3.781195810737537E-5 82-83 7.562391621475075E-5 2.2687174864425224E-4 0.0 2.0807353367117054 3.781195810737537E-5 84-85 7.562391621475075E-5 2.2687174864425224E-4 0.0 2.304355256958724 3.781195810737537E-5 86-87 7.562391621475075E-5 2.2687174864425224E-4 0.0 2.5454632078304025 3.781195810737537E-5 88 7.562391621475075E-5 2.2687174864425224E-4 0.0 2.742992876983332 3.781195810737537E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGATA 130 3.18179E-4 21.67143 8 CCGATAC 130 0.008089014 18.0585 9 CCTATAC 815 0.0 17.8892 3 GAGTACT 3740 0.0 17.826733 12-13 CCCCTAT 845 0.0 17.672651 1 GTATCAA 10825 0.0 17.382053 1 CAACGCA 11690 0.0 15.891968 5 TCAACGC 11770 0.0 15.863869 4 ATCAACG 11835 0.0 15.85592 3 TATACTG 1580 0.0 15.776616 5 AACGCAG 11760 0.0 15.65307 6 CTAGGAC 945 0.0 15.428251 3 TATCAAC 11960 0.0 15.425381 2 AGTACTT 4440 0.0 15.069087 12-13 TAGGGCG 190 0.0040111663 14.852265 5 CATGGGG 4175 0.0 14.757401 4 CTATACA 1290 0.0 14.583621 4 GTACTTT 4600 0.0 14.54412 14-15 TAGGGGT 680 0.0 14.524643 4 ACGCAGA 13040 0.0 14.150977 7 >>END_MODULE