FastQCFastQC Report
Wed 25 May 2016
SRR1295109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311479
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4350.022.4495811
GGTACCG1403.4698873E-421.3948215
ACACCGT3001.9790605E-919.9892736
GTACATG45200.019.5797461
TCGACGC1556.892431E-419.3243565
TACACCG2652.3786015E-718.838215
GTACCGG1650.007046201318.4953941
TACATGG48350.018.1733882
GTATAAG9250.017.8155631
CCGTGCA3501.3220415E-817.1510689
ACATGGG49750.017.0276363
GTATACG2200.001875110916.6458551
ATCAACG28700.016.5913
TCAACGC28750.016.5630784
GTATATA16250.016.5263461
GTATCAA29850.016.3577351
CAACGCA28850.016.0924665
AACGCAG29650.015.8430996
CCCGTGC3803.652349E-815.7938288
AGCGCTC1950.003206282915.3603845
Produced by FastQC (version 0.11.5)