##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295109_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2311479 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.433525461403715 33.0 27.0 33.0 15.0 33.0 2 22.611417624819435 27.0 15.0 33.0 2.0 33.0 3 24.947439280218422 27.0 15.0 33.0 15.0 33.0 4 25.227809121346116 33.0 15.0 37.0 2.0 37.0 5 26.945095326412222 33.0 15.0 37.0 2.0 37.0 6 27.52610774313762 33.0 15.0 37.0 6.0 37.0 7 27.431114883587522 33.0 15.0 37.0 6.0 37.0 8 28.379493821920942 33.0 22.0 37.0 6.0 37.0 9 29.2850776494184 33.0 27.0 37.0 15.0 37.0 10-11 28.81447441227024 33.0 24.5 37.0 10.5 37.0 12-13 27.633814973010786 33.0 15.0 37.0 4.0 37.0 14-15 29.309583171640323 33.0 24.5 37.0 10.5 40.0 16-17 29.727046838842142 33.0 27.0 37.0 10.5 40.0 18-19 29.844510808880376 33.0 27.0 38.5 10.5 40.0 20-21 29.364365196482424 33.0 24.5 37.0 6.0 40.0 22-23 30.087722189991773 33.0 27.0 37.0 15.0 40.0 24-25 30.16510467973103 33.0 27.0 37.0 15.0 40.0 26-27 28.85498224297084 33.0 22.0 37.0 4.0 40.0 28-29 29.523048662782575 33.0 24.5 37.0 10.5 40.0 30-31 30.222634296050277 33.0 27.0 37.0 15.0 40.0 32-33 29.06959288836282 33.0 22.0 37.0 6.0 40.0 34-35 28.59100169199028 33.0 22.0 37.0 6.0 40.0 36-37 28.85673804520828 33.0 22.0 37.0 6.0 40.0 38-39 28.965969191154237 33.0 24.5 37.0 6.0 40.0 40-41 28.101485023225386 33.0 22.0 37.0 6.0 40.0 42-43 28.672294016082343 33.0 22.0 37.0 6.0 40.0 44-45 28.444834021853538 33.0 22.0 37.0 6.0 40.0 46-47 28.18945856743669 33.0 22.0 37.0 6.0 40.0 48-49 27.978764678372592 33.0 22.0 37.0 6.0 40.0 50-51 27.937063239596814 33.0 22.0 37.0 6.0 40.0 52-53 28.13969043197018 33.0 22.0 37.0 6.0 40.0 54-55 28.094813537133582 33.0 22.0 37.0 6.0 40.0 56-57 28.08495404890116 33.0 22.0 37.0 6.0 40.0 58-59 27.87460344653791 33.0 22.0 37.0 6.0 40.0 60-61 27.86357436083131 33.0 22.0 37.0 6.0 40.0 62-63 27.76287606333434 33.0 22.0 37.0 6.0 40.0 64-65 27.355530376871258 33.0 22.0 37.0 6.0 40.0 66-67 27.581444391231763 33.0 22.0 37.0 6.0 37.0 68-69 27.181266626259635 33.0 22.0 37.0 6.0 37.0 70-71 27.277716994184242 33.0 22.0 37.0 6.0 37.0 72-73 27.299675445894167 33.0 22.0 37.0 10.5 37.0 74-75 27.404090627689023 30.0 22.0 37.0 10.5 37.0 76-77 27.18132070418983 27.0 22.0 35.0 10.5 37.0 78-79 26.751411325822126 27.0 22.0 33.0 6.0 37.0 80-81 26.562542640447955 27.0 22.0 33.0 6.0 37.0 82-83 26.640323576376858 27.0 22.0 33.0 10.5 37.0 84-85 26.58520670099101 27.0 22.0 33.0 10.5 37.0 86-87 26.36947101833934 27.0 22.0 33.0 10.5 37.0 88-89 26.30813950721594 27.0 22.0 33.0 10.5 37.0 90-91 26.429932523721824 27.0 22.0 33.0 15.0 37.0 92-93 26.36379434985133 27.0 22.0 33.0 10.5 37.0 94-95 26.23662036297972 27.0 22.0 33.0 10.5 37.0 96-97 26.335318209683066 27.0 22.0 33.0 10.5 37.0 98-99 26.400874505024706 27.0 22.0 33.0 15.0 37.0 100 26.0634723482238 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.380135868779455 1101 2 11.357258568073089 1101 3 8.455985396421742 1101 4 12.830048393752786 1101 5 10.7313303737346 1101 6 10.017830112418128 1101 7 9.65169012186075 1101 8 9.29462706562543 1101 9 7.545830427468889 1101 10-11 7.877224256416888 1101 12-13 9.368461894050668 1101 14-15 8.11183457122019 1101 16-17 8.32175719040234 1101 18-19 8.320750619965416 1101 20-21 7.26974912248712 1101 22-23 7.485040208630995 1101 24-25 7.46291916007144 1101 26-27 8.664202550667184 1101 28-29 8.307797031455248 1101 30-31 5.5955320235616846 1101 32-33 7.090651294362175 1101 34-35 8.88229724772832 1101 36-37 8.326730129328887 1101 38-39 8.170332200857594 1101 40-41 9.016285304736389 1101 42-43 8.62759398664722 1101 44-45 8.420599402671055 1101 46-47 7.386222165494669 1101 48-49 5.425216365543587 1101 50-51 6.821612355389952 1101 52-53 5.914642472701278 1101 54-55 4.19030991608442 1101 56-57 6.329220349001083 1101 58-59 6.274321236106971 1101 60-61 6.773192811410322 1101 62-63 6.261573458359706 1101 64-65 6.6484896002848615 1101 66-67 5.839059225120547 1101 68-69 6.672668394517363 1101 70-71 6.305075140013315 1101 72-73 5.461717980946851 1101 74-75 4.930851284259486 1101 76-77 5.050012425206678 1101 78-79 5.093922458580931 1101 80-81 4.834643469858893 1101 82-83 4.4751614049080395 1101 84-85 4.211981251384625 1101 86-87 4.046457373217621 1101 88-89 3.843815619862678 1101 90-91 3.881826677438827 1101 92-93 3.930058440539419 1101 94-95 3.7987535113890267 1101 96-97 3.838098958355541 1101 98-99 3.5450356639171936 1101 100 3.64188795681709 1102 1 6.798279323004049 1102 2 10.381536233663077 1102 3 8.105650676371791 1102 4 12.479159958170907 1102 5 10.472508088365906 1102 6 9.491817765227157 1102 7 6.578384120026776 1102 8 7.731018350980992 1102 9 6.353410773319002 1102 10-11 7.279080435326534 1102 12-13 8.975823307361459 1102 14-15 5.8873295028078125 1102 16-17 7.0535727425254215 1102 18-19 7.203296765474086 1102 20-21 6.678135565933957 1102 22-23 6.3744652850209 1102 24-25 6.908128657506705 1102 26-27 8.316101881263052 1102 28-29 7.624091194495069 1102 30-31 4.101163287142686 1102 32-33 4.247515595571915 1102 34-35 4.814526728737356 1102 36-37 4.8635207408743355 1102 38-39 4.582623085124574 1102 40-41 5.9876911577199685 1102 42-43 6.659665859246964 1102 44-45 6.48923737935602 1102 46-47 5.549881953608111 1102 48-49 3.554313424377167 1102 50-51 4.665051203992544 1102 52-53 5.592548072025959 1102 54-55 5.140107555447102 1102 56-57 6.145505538216593 1102 58-59 5.788981030155007 1102 60-61 5.962723613477795 1102 62-63 4.121232125734206 1102 64-65 6.135016545046565 1102 66-67 3.4573720670228063 1102 68-69 5.885196932267757 1102 70-71 5.169292671988636 1102 72-73 4.8799894245654265 1102 74-75 4.229698372631152 1102 76-77 3.3837860873740198 1102 78-79 4.326903906502988 1102 80-81 4.428910257159654 1102 82-83 3.729513125229726 1102 84-85 2.86149273545195 1102 86-87 3.042589187367021 1102 88-89 3.2218367459355584 1102 90-91 3.1008193180037154 1102 92-93 2.967109023235551 1102 94-95 2.911118650005836 1102 96-97 3.154354240817902 1102 98-99 2.7973173082023486 1102 100 2.7555210302691897 1103 1 6.712016362215188 1103 2 10.943229118362193 1103 3 7.981507753285705 1103 4 11.823872321467473 1103 5 10.202993833365412 1103 6 8.749446020396949 1103 7 7.861942270245137 1103 8 8.041090785522588 1103 9 7.042192686463569 1103 10-11 7.343765899159699 1103 12-13 8.79006554601056 1103 14-15 8.522156894229301 1103 16-17 7.983892694177943 1103 18-19 7.246800755302964 1103 20-21 8.386813990096776 1103 22-23 6.716909082643841 1103 24-25 5.187054755537236 1103 26-27 5.747000173222737 1103 28-29 6.921417809048403 1103 30-31 6.160143449664421 1103 32-33 7.972071120944101 1103 34-35 7.151957149474395 1103 36-37 4.0096780881660266 1103 38-39 6.302163297203585 1103 40-41 6.721453119414885 1103 42-43 7.408404916932163 1103 44-45 5.678757893562523 1103 46-47 6.296899360803025 1103 48-49 5.42525866591447 1103 50-51 6.352687022215903 1103 52-53 5.985653761319636 1103 54-55 4.8918402987848 1103 56-57 5.718493302151213 1103 58-59 5.253859581456794 1103 60-61 4.869430590611405 1103 62-63 2.407317232125031 1103 64-65 4.871032687675317 1103 66-67 3.9683377385789917 1103 68-69 5.018593410920342 1103 70-71 3.6047560348863 1103 72-73 4.363700514074839 1103 74-75 3.171771892298505 1103 76-77 3.5025949092029762 1103 78-79 2.920740377461584 1103 80-81 3.9390281696093297 1103 82-83 3.3503154322469157 1103 84-85 3.168779011875383 1103 86-87 2.6794717892639994 1103 88-89 2.842144291672117 1103 90-91 2.5470798112564452 1103 92-93 2.122675115305629 1103 94-95 2.8486680399176727 1103 96-97 2.7425479103152277 1103 98-99 2.6713648217355335 1103 100 2.7169957464149377 1104 1 6.268252054693864 1104 2 9.606725817668089 1104 3 7.431430089894995 1104 4 12.072791865499521 1104 5 9.404046466071733 1104 6 8.122534347356765 1104 7 3.812944527318308 1104 8 6.532724758390589 1104 9 6.7476499549215525 1104 10-11 7.253913555992781 1104 12-13 7.563591884701893 1104 14-15 7.17275968821416 1104 16-17 7.026938271082603 1104 18-19 6.734714581375528 1104 20-21 7.438381559694712 1104 22-23 6.383822144934594 1104 24-25 6.003448922031264 1104 26-27 8.28253434924568 1104 28-29 7.267395113926792 1104 30-31 6.397216515217156 1104 32-33 7.656008221094687 1104 34-35 8.033088365108423 1104 36-37 6.483730812061047 1104 38-39 7.325202422814179 1104 40-41 7.070519747290657 1104 42-43 7.162041486841922 1104 44-45 6.699437320475244 1104 46-47 6.642880911463699 1104 48-49 5.376898574462615 1104 50-51 5.527308264010074 1104 52-53 5.924565158804725 1104 54-55 4.543711051663749 1104 56-57 5.9870187213055495 1104 58-59 3.8520074947198246 1104 60-61 4.246381661451249 1104 62-63 3.762291177556861 1104 64-65 2.731880443408347 1104 66-67 2.9381493084657926 1104 68-69 4.02337360715352 1104 70-71 2.353253467832772 1104 72-73 2.3699664663586724 1104 74-75 2.9898094060418323 1104 76-77 3.724708362472903 1104 78-79 4.297590983912297 1104 80-81 2.909940315117108 1104 82-83 2.841487360725605 1104 84-85 2.944088961632861 1104 86-87 0.6340578882208696 1104 88-89 0.17885158116775912 1104 90-91 2.2493071868978625 1104 92-93 2.407055394802036 1104 94-95 2.3592709249382153 1104 96-97 2.7684188197143627 1104 98-99 2.4770009791541447 1104 100 1.0641202731440877 1105 1 5.691634439149009 1105 2 -2.468916880355412 1105 3 0.5515857153871266 1105 4 8.309411823338234 1105 5 8.400375107341954 1105 6 7.982319715993949 1105 7 5.240493516145065 1105 8 5.889115046841585 1105 9 4.707853385634511 1105 10-11 4.937581476035838 1105 12-13 4.958642480740927 1105 14-15 5.751536761642576 1105 16-17 5.921587375528976 1105 18-19 6.0723624698225365 1105 20-21 6.930854137414016 1105 22-23 1.8744246252742762 1105 24-25 3.868678566500961 1105 26-27 6.768995143233461 1105 28-29 5.710850364901084 1105 30-31 5.159944328212408 1105 32-33 6.922103704454436 1105 34-35 6.047773955765162 1105 36-37 6.114980718618472 1105 38-39 3.918252849410674 1105 40-41 5.097685683859343 1105 42-43 5.492461436457681 1105 44-45 6.021876962801986 1105 46-47 5.369982341508848 1105 48-49 4.980491036512472 1105 50-51 5.959213545633531 1105 52-53 5.445751237854651 1105 54-55 5.126760008097079 1105 56-57 5.035616490893606 1105 58-59 1.94191996394607 1105 60-61 4.360204060757532 1105 62-63 -0.8549783183017645 1105 64-65 -0.6872643203189881 1105 66-67 0.4283482726237118 1105 68-69 2.4719222570952084 1105 70-71 2.6918840606822982 1105 72-73 2.681936024013986 1105 74-75 2.5983470048417807 1105 76-77 1.7553964611283277 1105 78-79 3.1855433705602216 1105 80-81 1.3460170289785047 1105 82-83 0.7026444663481044 1105 84-85 1.0956134149315098 1105 86-87 -0.5490672862052683 1105 88-89 1.5586716606064037 1105 90-91 1.4191748846721843 1105 92-93 1.506643531083668 1105 94-95 0.888883343830063 1105 96-97 1.9660768061888625 1105 98-99 1.81209554926847 1105 100 2.017658133583243 1106 1 6.107932419058571 1106 2 10.281969164597676 1106 3 6.750491070136512 1106 4 11.614644413590543 1106 5 9.765250260155945 1106 6 9.402533784454615 1106 7 6.408865991952588 1106 8 7.376478895102817 1106 9 6.882501361125353 1106 10-11 5.654509675555669 1106 12-13 7.943106545519328 1106 14-15 7.817110532134382 1106 16-17 7.7373894464346655 1106 18-19 7.26738625725276 1106 20-21 8.178873539266931 1106 22-23 6.122025169513005 1106 24-25 5.017256107631862 1106 26-27 7.645398086099441 1106 28-29 6.117565020682978 1106 30-31 5.403674812209285 1106 32-33 7.281564086968096 1106 34-35 6.664590812804914 1106 36-37 5.90478254833582 1106 38-39 6.997604273710678 1106 40-41 6.4596822262127525 1106 42-43 6.069435268443307 1106 44-45 4.242565445046107 1106 46-47 5.8647261387338965 1106 48-49 5.790218061021623 1106 50-51 6.448944629839559 1106 52-53 5.609168996312814 1106 54-55 5.206952829890199 1106 56-57 4.788980930158331 1106 58-59 4.904769364841599 1106 60-61 4.728884373897326 1106 62-63 4.95191497334833 1106 64-65 5.188299986752433 1106 66-67 2.9811043096967538 1106 68-69 3.1013906853573268 1106 70-71 2.6870082818833048 1106 72-73 2.7356616474214057 1106 74-75 2.8296912708191293 1106 76-77 3.3904808034021983 1106 78-79 4.155487805185295 1106 80-81 3.653654368247647 1106 82-83 2.1635263871646835 1106 84-85 2.6022012166931 1106 86-87 3.40749510792962 1106 88-89 3.521864202547402 1106 90-91 3.3611621845410973 1106 92-93 3.068021130762574 1106 94-95 1.517969046700756 1106 96-97 1.5123101715578997 1106 98-99 1.9669455982425923 1106 100 2.8921923270936176 1107 1 5.842499188241565 1107 2 10.164222867136498 1107 3 5.912935127826856 1107 4 10.894029829130108 1107 5 8.99439106085919 1107 6 7.460999878570348 1107 7 3.364345052519134 1107 8 5.884226457971245 1107 9 5.352147328296901 1107 10-11 4.211382930653826 1107 12-13 5.750167090060131 1107 14-15 5.81033342047839 1107 16-17 6.759409290098798 1107 18-19 5.985092162547723 1107 20-21 5.214673790147671 1107 22-23 5.487831175857156 1107 24-25 4.239927513768286 1107 26-27 6.727555645196098 1107 28-29 5.967170069963558 1107 30-31 4.714540060397216 1107 32-33 4.462799671564852 1107 34-35 6.245173413638614 1107 36-37 6.187812504865718 1107 38-39 6.032898361691323 1107 40-41 7.312508867864704 1107 42-43 5.654996666064221 1107 44-45 3.6157175378279653 1107 46-47 3.114442797545255 1107 48-49 3.553605244463494 1107 50-51 4.335756014470178 1107 52-53 3.6425941633679635 1107 54-55 4.983869555089591 1107 56-57 4.973833164824743 1107 58-59 2.6875521237595095 1107 60-61 2.3652525603364403 1107 62-63 0.10645703652455296 1107 64-65 1.7410498175729856 1107 66-67 3.659165066972694 1107 68-69 4.537385607223012 1107 70-71 3.8948898587152243 1107 72-73 2.079579164875774 1107 74-75 2.0647622161517027 1107 76-77 2.6552020134005048 1107 78-79 3.572669804369813 1107 80-81 1.838070205522392 1107 82-83 1.258304320418425 1107 84-85 2.0044226788558994 1107 86-87 2.0394785359851006 1107 88-89 2.2231520600784407 1107 90-91 0.9733538332200276 1107 92-93 1.8801968701547018 1107 94-95 1.9034177497194555 1107 96-97 1.8645475888555865 1107 98-99 1.3797401645716327 1107 100 -0.32088345612928393 1108 1 4.085156470053114 1108 2 -3.1110626669587464 1108 3 0.01338027564866806 1108 4 6.12636720810022 1108 5 6.397403170997379 1108 6 6.379424380197669 1108 7 3.8385706224768903 1108 8 4.637189495977353 1108 9 4.50664133772435 1108 10-11 3.003186272183086 1108 12-13 2.1371240489630594 1108 14-15 3.5630169481227654 1108 16-17 4.277994333351028 1108 18-19 3.4990660073751307 1108 20-21 4.2512217284184 1108 22-23 3.5038616845462407 1108 24-25 2.5578819207986534 1108 26-27 4.099605308749286 1108 28-29 5.685275998230015 1108 30-31 4.0850851596032385 1108 32-33 4.111327674928408 1108 34-35 2.4848270428772103 1108 36-37 0.8365374964842118 1108 38-39 1.890053127225105 1108 40-41 4.749676839977862 1108 42-43 2.6733452473532857 1108 44-45 3.1147310754084607 1108 46-47 4.404483512507664 1108 48-49 4.624702793047337 1108 50-51 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22-23 -18.59555649568498 2212 24-25 -25.039340301423568 2212 26-27 -17.06084275762965 2212 28-29 -18.00153058350979 2212 30-31 -6.017711799046037 2212 32-33 -17.1980111816414 2212 34-35 -13.67255925590736 2212 36-37 -17.58521469520121 2212 38-39 -23.551283314111338 2212 40-41 -10.149513184015778 2212 42-43 -13.803287583462577 2212 44-45 -16.98413884013467 2212 46-47 -16.880834468804032 2212 48-49 -17.493017707626812 2212 50-51 -8.95449898654174 2212 52-53 -2.0839827109465254 2212 54-55 -23.292461690016125 2212 56-57 -23.193844987093815 2212 58-59 -12.393854228028516 2212 60-61 -6.222090215132138 2212 62-63 -3.2545261662022824 2212 64-65 -17.11720035266965 2212 66-67 -17.39849728175435 2212 68-69 -14.462115967867367 2212 70-71 -9.517092623671504 2212 72-73 -17.4086900104117 2212 74-75 -6.243956651899342 2212 76-77 -11.271264703011255 2212 78-79 -4.983469126537209 2212 80-81 -2.57543200378716 2212 82-83 -3.1297274686640257 2212 84-85 -5.658777876501102 2212 86-87 -4.9234470167728865 2212 88-89 -3.424746804417037 2212 90-91 -4.327437602791193 2212 92-93 -5.356824243622739 2212 94-95 -3.5382477737943283 2212 96-97 -5.377623556817358 2212 98-99 -3.6824830565850846 2212 100 -3.8448033919568836 2213 1 -21.027508642612 2213 2 -16.81502537092145 2213 3 -19.859774347505954 2213 4 -18.829149497416484 2213 5 -20.887950364356158 2213 6 -21.512766761993472 2213 7 -10.531157427251161 2213 8 -10.234430550642692 2213 9 -4.253362547279536 2213 10-11 -17.49269368119687 2213 12-13 -16.132638965799956 2213 14-15 -23.456796571690756 2213 16-17 -17.832862310005613 2213 18-19 -18.378418452609328 2213 20-21 -17.54688433218766 2213 22-23 -19.677653466968913 2213 24-25 -7.406562523645793 2213 26-27 -23.114652655508678 2213 28-29 -24.025096962142943 2213 30-31 -19.35547233845426 2213 32-33 -23.234146295545564 2213 34-35 -17.726960058909892 2213 36-37 -23.608781073834358 2213 38-39 -11.400172203000228 2213 40-41 -16.945917565616547 2213 42-43 -23.121040922032883 2213 44-45 -16.957073508896126 2213 46-47 -16.82926163123748 2213 48-49 -5.818362475017928 2213 50-51 -23.196839913485967 2213 52-53 -17.66139891937805 2213 54-55 -17.45440613446057 2213 56-57 -17.317178320427146 2213 58-59 -17.795570864146175 2213 60-61 -19.164575813456306 2213 62-63 -17.25744425121581 2213 64-65 -17.45527794715337 2213 66-67 -8.61826467286895 2213 68-69 -22.558738120263282 2213 70-71 -17.241268455040974 2213 72-73 -9.33306135544094 2213 74-75 -4.177541359938093 2213 76-77 -2.9414877107794304 2213 78-79 -4.419865434471223 2213 80-81 -3.231657237268873 2213 82-83 -3.0943288040921466 2213 84-85 -2.8831116898902955 2213 86-87 -3.04779763211722 2213 88-89 -2.648589869791916 2213 90-91 -2.6290772117638745 2213 92-93 -3.674500336840861 2213 94-95 -3.7159738364634407 2213 96-97 -3.2800408562849306 2213 98-99 -3.5511919245260835 2213 100 -3.2936416567731506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 598.0 3 604.0 4 2504.0 5 8781.0 6 14544.0 7 17856.0 8 16344.0 9 16946.0 10 20724.0 11 26511.0 12 33953.0 13 43533.0 14 36398.0 15 41260.0 16 46004.0 17 48696.0 18 49121.0 19 49764.0 20 49998.0 21 49920.0 22 52502.0 23 56628.0 24 61891.0 25 67439.0 26 73566.0 27 80023.0 28 85546.0 29 89150.0 30 94972.0 31 103811.0 32 118124.0 33 139816.0 34 163384.0 35 181603.0 36 185242.0 37 144090.0 38 39565.0 39 68.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.54975007134842 21.252813266065946 14.003348495454619 24.19408816713101 2 14.816275765240189 21.88257946410332 40.29941457898104 23.001730191675442 3 17.395677737750052 26.91226971853674 30.654801130539205 25.037251413174005 4 12.418455185734029 18.022500097126716 39.76634087591646 29.792703841222796 5 12.890353480525768 37.91739559984693 36.00497106395608 13.18727985567123 6 29.694532540782802 38.59600812807414 18.664137721480525 13.045321609662533 7 25.600982292781865 31.90304759042386 25.00963718450471 17.48633293228956 8 23.232173756266373 37.230033647013094 22.09819682917292 17.43959576754761 9 24.07764125940798 17.59684229435931 22.586120375988873 35.73939607024384 10-11 22.799662452697902 27.694881184961417 29.69119947871276 19.81425688362792 12-13 23.612931950830912 25.379343148207195 29.443790024485704 21.56393487647619 14-15 21.06457207855492 27.046314232642 26.86579044061503 25.02332324818805 16-17 20.795915363359793 30.55320112724214 27.537137763937224 21.11374574546084 18-19 20.694158945732866 29.68719571847845 29.03785463543765 20.58079070035104 20-21 22.074286389748288 28.984354930584256 28.09015484048712 20.85120383918034 22-23 21.2220045608705 28.976190215985632 28.69885413592997 21.1029510872139 24-25 21.457837519449523 28.87801644938811 28.255313933178762 21.408832097983606 26-27 21.33565732918334 28.906856380613604 28.31160515784793 21.445881132355122 28-29 21.209220580463313 28.766419118294724 28.79956472591679 21.22479557532517 30-31 21.75896882601116 29.124542207580145 28.131847808106613 20.98464115830208 32-33 21.02275683771602 29.036660799126196 28.630956705989348 21.30962565716844 34-35 21.079964963471276 28.726234617236084 28.450802412272395 21.74299800702024 36-37 21.537641509673 29.207398410295877 28.051756715886057 21.20320336414507 38-39 21.369753178074994 28.95306842712414 28.25997966945736 21.417198725343507 40-41 21.437213011401525 28.845300273428542 27.989422049540874 21.728064665629056 42-43 21.271257081488557 28.948877490305318 28.443579534480868 21.33628589372526 44-45 21.618239348975195 28.57737578588191 28.376272680930796 21.428112184212097 46-47 21.515386368816962 28.916001823569637 28.369751538636883 21.19886026897652 48-49 21.437411770773885 28.675958930451547 28.44621254848052 21.44041675029405 50-51 21.674866192527674 28.718612106858217 28.211408098006824 21.39511360260728 52-53 21.209957812896686 28.75281562453298 28.40753588064453 21.629690681925805 54-55 21.659214888227847 28.71977118710044 28.22878212080119 21.39223180387052 56-57 21.588266122109676 28.695683602750492 28.203571848090288 21.512478427049544 58-59 21.399237776101266 28.331538508564435 28.450312689153595 21.818911026180704 60-61 21.762579305918685 28.706558725509286 28.085517565364153 21.445344403207876 62-63 21.434793457290148 28.78680942642125 28.3179835407449 21.460413575543704 64-65 21.46617422195496 28.358030058630174 28.37062559168532 21.80517012772954 66-67 21.458052759957454 28.584096824628098 28.15010647966935 21.8077439357451 68-69 21.948418120491585 28.44722456345592 28.106914064120065 21.49744325193243 70-71 21.937556908863122 28.49936164783745 28.003052238743265 21.56002920455616 72-73 21.619187984346063 28.66848305922564 28.31545566395758 21.396873292470715 74-75 21.749593352950487 28.37344009800014 28.041179040280312 21.83578750876906 76-77 21.775959356240932 28.656120743578185 28.055044609669487 21.5128752905114 78-79 21.809249223873064 28.409726505978323 28.170953692392185 21.610070577756424 80-81 21.89305213452048 28.363584184459473 28.056791431987083 21.686572249032963 82-83 21.52013320564165 28.475622496952813 28.488551279818907 21.515693017586628 84-85 21.64252246770063 28.232319390637663 28.099904437409123 22.025253704252577 86-87 21.802822512573456 28.486077302942075 28.124464307180748 21.586635877303724 88-89 21.61292890762686 28.15164528337019 28.38289463895648 21.852531170046475 90-91 21.834398058260845 28.166122236260538 28.134015728503382 21.86546397697523 92-93 21.563676339474995 28.37116379275757 28.3246442665931 21.74051560117433 94-95 21.48704878244866 27.98458152172732 28.330432590873635 22.197937104950384 96-97 21.620119053337103 28.216279866841436 28.322818889529554 21.840782190291907 98-99 21.51555028417871 28.06231363973103 28.333493689949073 22.088642386141192 100 21.7840951980183 27.906979770854935 28.101202122259867 22.2077229088669 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 253.0 1 308.5 2 308.5 3 248.5 4 262.0 5 363.0 6 558.5 7 760.0 8 1060.5 9 1534.0 10 2100.0 11 2748.5 12 3425.5 13 4086.5 14 4730.0 15 5390.5 16 5997.5 17 6627.0 18 7415.0 19 8138.5 20 8982.5 21 9979.5 22 11188.5 23 12765.5 24 14851.5 25 17771.5 26 21532.0 27 26521.0 28 32605.5 29 39451.0 30 46430.5 31 53384.5 32 60378.5 33 66814.0 34 72720.0 35 78121.0 36 82600.0 37 86022.0 38 88475.5 39 90006.0 40 90580.5 41 89843.5 42 87721.5 43 84639.5 44 81225.5 45 77542.0 46 73724.0 47 70036.0 48 66321.5 49 63113.5 50 60281.5 51 56672.5 52 52131.5 53 47641.5 54 43890.0 55 40505.5 56 37050.0 57 33497.5 58 29995.0 59 26521.0 60 22977.5 61 19493.5 62 16160.5 63 13308.0 64 10902.5 65 8733.5 66 6946.0 67 5429.5 68 4243.0 69 3324.0 70 2522.5 71 1934.5 72 1531.0 73 1186.5 74 878.0 75 633.0 76 461.5 77 339.0 78 243.0 79 163.0 80 106.0 81 71.0 82 46.5 83 33.5 84 23.5 85 16.5 86 14.5 87 10.0 88 5.0 89 2.0 90 1.5 91 1.0 92 0.5 93 2.0 94 2.0 95 1.0 96 1.5 97 1.0 98 0.0 99 1.0 100 3.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 6.92478711682001 2 23.336616945254534 3 5.465202149792406 4 22.051206175786152 5 11.370339077274766 6 8.877389757813072 7 7.299958165313204 8 7.617373984362392 9 4.391993178393574 10-11 6.053764710819349 12-13 8.954699566814147 14-15 6.51292960048523 16-17 6.97715618441699 18-19 6.028780707071101 20-21 7.038220983188685 22-23 4.992777351643688 24-25 4.700951209160888 26-27 8.784051250303378 28-29 5.697780511957928 30-31 3.6255791205544154 32-33 7.712551141498582 34-35 6.354416371509323 36-37 6.092354721803659 38-39 5.306299559719124 40-41 7.258318159066121 42-43 5.696612428665802 44-45 5.796678230691259 46-47 5.1040481008047225 48-49 3.5408714507032077 50-51 5.991293885862688 52-53 3.8913180695130696 54-55 4.419810865683833 56-57 4.242262205280689 58-59 3.4034702456738737 60-61 3.672778338025134 62-63 2.9050231475172392 64-65 4.170836075084394 66-67 2.1840561822106106 68-69 4.553729452008866 70-71 2.171250528341378 72-73 1.386666285958038 74-75 0.6203603839792617 76-77 0.9230670060164943 78-79 1.4981100844956843 80-81 1.7126264179774076 82-83 0.6177430121580165 84-85 1.0371281763753857 86-87 0.31235412478330976 88-89 0.5571324679999256 90-91 0.22033511877027653 92-93 0.2593793843681902 94-95 0.44750568791669754 96-97 0.4721435929117245 98-99 0.40314015398798775 100 0.27640311679232216 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2311479.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.91344459089424 #Duplication Level Percentage of deduplicated Percentage of total 1 97.81089489852587 94.79190743134066 2 1.818843180314592 3.525407155498881 3 0.2280347223702411 0.6629889129368494 4 0.06639741672378265 0.25739209466555296 5 0.024669476446289435 0.11954019693319207 6 0.016228368965891794 0.09436482819459252 7 0.008691826077675123 0.058964836348072036 8 0.006123881683828851 0.04747891745975516 9 0.004473632726312659 0.03901996416073617 >10 0.014443945619508226 0.21737272081347944 >50 5.71307729852296E-4 0.03629236696942271 >100 5.378598471234387E-4 0.09485312986511991 >500 8.948296901179098E-5 0.05441744481362232 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.3262344152812985E-5 0.0 0.0 0.0 0.0 7 4.3262344152812985E-5 0.0 0.0 4.3262344152812985E-5 0.0 8 4.3262344152812985E-5 0.0 0.0 4.3262344152812985E-5 0.0 9 4.3262344152812985E-5 0.0 0.0 4.3262344152812985E-5 0.0 10-11 4.3262344152812985E-5 0.0 0.0 4.3262344152812985E-5 2.1631172076406492E-5 12-13 4.3262344152812985E-5 0.0 0.0 4.3262344152812985E-5 4.3262344152812985E-5 14-15 4.3262344152812985E-5 0.0 0.0 4.3262344152812985E-5 4.3262344152812985E-5 16-17 4.3262344152812985E-5 0.0 0.0 4.3262344152812985E-5 4.3262344152812985E-5 18-19 4.3262344152812985E-5 0.0 0.0 4.3262344152812985E-5 4.3262344152812985E-5 20-21 4.3262344152812985E-5 0.0 0.0 4.3262344152812985E-5 6.489351622921948E-5 22-23 1.0815586038203246E-4 0.0 0.0 4.3262344152812985E-5 8.652468830562597E-5 24-25 1.2978703245843895E-4 0.0 0.0 1.9468054868765843E-4 8.652468830562597E-5 26-27 1.2978703245843895E-4 0.0 0.0 2.595740649168779E-4 8.652468830562597E-5 28-29 1.2978703245843895E-4 0.0 0.0 5.840416460629752E-4 8.652468830562597E-5 30-31 1.2978703245843895E-4 0.0 0.0 0.0017521249381889257 8.652468830562597E-5 32-33 1.2978703245843895E-4 0.0 0.0 0.005169850126261151 8.652468830562597E-5 34-35 1.2978703245843895E-4 0.0 0.0 0.01049111845705715 8.652468830562597E-5 36-37 1.2978703245843895E-4 0.0 0.0 0.018948906738932087 8.652468830562597E-5 38-39 1.2978703245843895E-4 0.0 0.0 0.03106236310171972 8.652468830562597E-5 40-41 1.2978703245843895E-4 0.0 0.0 0.04674496285711442 8.652468830562597E-5 42-43 1.2978703245843895E-4 0.0 0.0 0.06277366136573163 8.652468830562597E-5 44-45 1.2978703245843895E-4 0.0 0.0 0.08310696311755374 8.652468830562597E-5 46-47 1.2978703245843895E-4 0.0 0.0 0.10802607334957401 8.652468830562597E-5 48-49 1.2978703245843895E-4 0.0 0.0 0.13448099679901915 8.652468830562597E-5 50-51 1.2978703245843895E-4 0.0 0.0 0.16084939556015865 8.652468830562597E-5 52-53 1.2978703245843895E-4 0.0 0.0 0.19013800255161306 8.652468830562597E-5 54-55 1.2978703245843895E-4 0.0 0.0 0.22513723897123877 8.652468830562597E-5 56-57 1.7304937661125194E-4 0.0 0.0 0.26876731304935064 8.652468830562597E-5 58-59 1.7304937661125194E-4 0.0 0.0 0.31828106593224514 8.652468830562597E-5 60-61 1.7304937661125194E-4 0.0 0.0 0.3657614886399574 8.652468830562597E-5 62-63 1.7304937661125194E-4 0.0 0.0 0.4148858804254765 8.652468830562597E-5 64-65 1.7304937661125194E-4 0.0 0.0 0.47534500637903265 8.652468830562597E-5 66-67 1.7304937661125194E-4 0.0 0.0 0.5484367368252102 1.2978703245843895E-4 68-69 1.7304937661125194E-4 0.0 0.0 0.6286018605403726 1.2978703245843895E-4 70-71 1.7304937661125194E-4 0.0 0.0 0.723454550095415 1.2978703245843895E-4 72-73 2.1631172076406492E-4 0.0 0.0 0.8255969446402065 1.2978703245843895E-4 74-75 2.1631172076406492E-4 0.0 0.0 0.9337095426780861 1.2978703245843895E-4 76-77 2.1631172076406492E-4 0.0 0.0 1.0556228285007132 1.2978703245843895E-4 78-79 2.1631172076406492E-4 0.0 0.0 1.1984101953770725 1.2978703245843895E-4 80-81 2.1631172076406492E-4 0.0 0.0 1.362980152534373 1.2978703245843895E-4 82-83 2.1631172076406492E-4 0.0 0.0 1.5357483239086318 1.2978703245843895E-4 84-85 2.1631172076406492E-4 0.0 0.0 1.7188345643633363 1.2978703245843895E-4 86-87 2.1631172076406492E-4 0.0 0.0 1.9233140340016068 1.2978703245843895E-4 88 2.1631172076406492E-4 0.0 0.0 2.0885761886653524 1.2978703245843895E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 435 0.0 22.449581 1 GGTACCG 140 3.4698873E-4 21.394821 5 ACACCGT 300 1.9790605E-9 19.989273 6 GTACATG 4520 0.0 19.579746 1 TCGACGC 155 6.892431E-4 19.324356 5 TACACCG 265 2.3786015E-7 18.83821 5 GTACCGG 165 0.0070462013 18.495394 1 TACATGG 4835 0.0 18.173388 2 GTATAAG 925 0.0 17.815563 1 CCGTGCA 350 1.3220415E-8 17.151068 9 ACATGGG 4975 0.0 17.027636 3 GTATACG 220 0.0018751109 16.645855 1 ATCAACG 2870 0.0 16.591 3 TCAACGC 2875 0.0 16.563078 4 GTATATA 1625 0.0 16.526346 1 GTATCAA 2985 0.0 16.357735 1 CAACGCA 2885 0.0 16.092466 5 AACGCAG 2965 0.0 15.843099 6 CCCGTGC 380 3.652349E-8 15.793828 8 AGCGCTC 195 0.0032062829 15.360384 5 >>END_MODULE