FastQCFastQC Report
Wed 25 May 2016
SRR1295109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311479
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52050.024.2562391
ACCGCGT1100.003075585521.3454958
ATCAACG57950.021.1012063
TCAACGC58050.020.822254
CAACGCA58000.020.7591115
TATCAAC61550.020.0953082
AACGCAG60250.019.8773776
GCGTCAG2451.9508843E-719.196884
ATAGTCG1250.006444612518.7840378
CGCAGAG67300.017.9327558
TACGCAA1851.641919E-417.796035
AGCGTCA2654.4498302E-717.7476773
ACGCAGA68150.017.640187
TACACCG1600.001273235117.6371355
GAGTACT30450.017.5032512-13
GGTATCA39050.017.428591
GTACATG67300.017.0011061
CTATACT12300.016.8245854
TACATGG68900.016.4496882
CGTCAGA3201.7961247E-716.1673745
Produced by FastQC (version 0.11.5)