FastQCFastQC Report
Wed 25 May 2016
SRR1295108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780097
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG57150.020.9942341
GTATTAG10750.020.500021
GTATCAA34550.019.4896851
TACATGG60000.019.378822
GTATAAT12400.019.253211
ACATGGG63500.018.7449043
GTATAGG8200.017.9166831
ATCAACG34700.017.7924883
GTATTAC8700.017.5906141
TCAACGC34950.017.507314
TATCAAC38000.017.2315832
CAACGCA35050.016.730795
AACGCAG36500.016.2483436
GTATAAG8350.016.128591
TCTGTCG6550.016.127898
CATGGGG38750.015.9340134
TATACAG14350.015.7173465
GTATTGG11050.015.5115781
GTGTATA13050.015.479741
CAACGCG2000.00369563915.0362615
Produced by FastQC (version 0.11.5)