##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295108_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2780097
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.22016426045566	27.0	27.0	33.0	15.0	33.0
2	22.42564845758979	27.0	6.0	33.0	2.0	33.0
3	24.652363568609296	27.0	15.0	33.0	15.0	33.0
4	24.889914272775375	33.0	15.0	37.0	2.0	37.0
5	26.704223629607167	33.0	15.0	37.0	2.0	37.0
6	27.736200571418912	33.0	15.0	37.0	6.0	37.0
7	27.403996695079343	33.0	15.0	37.0	6.0	37.0
8	28.151708735342687	33.0	22.0	37.0	6.0	37.0
9	29.25776186945995	33.0	27.0	37.0	15.0	37.0
10-11	28.837721849273606	33.0	24.5	37.0	10.5	37.0
12-13	27.625297966222043	33.0	15.0	37.0	4.0	37.0
14-15	29.315690963300923	33.0	24.5	37.0	10.5	40.0
16-17	29.783510611320395	33.0	27.0	37.0	6.0	40.0
18-19	29.928047474602504	33.0	27.0	38.5	10.5	40.0
20-21	29.43320628740652	33.0	24.5	37.0	6.0	40.0
22-23	30.178906707212015	33.0	27.0	37.0	15.0	40.0
24-25	30.293289946357987	33.0	27.0	37.0	15.0	40.0
26-27	28.943809694409943	33.0	22.0	37.0	4.0	40.0
28-29	29.6247949262202	33.0	27.0	37.0	10.5	40.0
30-31	30.32562011325504	33.0	27.0	37.0	15.0	40.0
32-33	29.157566084924376	33.0	24.5	37.0	6.0	40.0
34-35	28.66504154351449	33.0	22.0	37.0	6.0	40.0
36-37	28.933048379247197	33.0	22.0	37.0	6.0	40.0
38-39	29.063575839260285	33.0	24.5	37.0	6.0	40.0
40-41	28.212683406370353	33.0	22.0	37.0	6.0	40.0
42-43	28.805262550191593	33.0	22.0	37.0	6.0	40.0
44-45	28.604559661047798	33.0	22.0	37.0	6.0	40.0
46-47	28.35160391885607	33.0	22.0	37.0	6.0	40.0
48-49	28.158907045329713	33.0	22.0	37.0	6.0	40.0
50-51	28.10958574467006	33.0	22.0	37.0	6.0	40.0
52-53	28.305064355668165	33.0	22.0	37.0	6.0	40.0
54-55	28.263017981027282	33.0	22.0	37.0	6.0	40.0
56-57	28.22604409126732	33.0	22.0	37.0	6.0	40.0
58-59	28.076202556961142	33.0	22.0	37.0	6.0	40.0
60-61	28.04631511058787	33.0	22.0	37.0	6.0	40.0
62-63	27.948853223466664	33.0	22.0	37.0	6.0	40.0
64-65	27.510400896083844	33.0	22.0	37.0	6.0	40.0
66-67	27.725077398378545	33.0	22.0	37.0	10.5	37.0
68-69	27.312309066913855	33.0	22.0	37.0	6.0	37.0
70-71	27.411328273797643	33.0	22.0	37.0	10.5	37.0
72-73	27.403004103813643	33.0	22.0	37.0	10.5	37.0
74-75	27.498548252093364	30.0	22.0	37.0	15.0	37.0
76-77	27.275321328716227	27.0	22.0	35.0	10.5	37.0
78-79	26.865438328230994	27.0	22.0	33.0	6.0	37.0
80-81	26.648904696490806	27.0	22.0	33.0	6.0	37.0
82-83	26.71688361952838	27.0	22.0	33.0	10.5	37.0
84-85	26.662333904176727	27.0	22.0	33.0	10.5	37.0
86-87	26.44096626844315	27.0	22.0	33.0	10.5	37.0
88-89	26.394136607463693	27.0	22.0	33.0	10.5	37.0
90-91	26.530746589057863	27.0	22.0	33.0	15.0	37.0
92-93	26.469750515899264	27.0	22.0	33.0	15.0	37.0
94-95	26.357565401494984	27.0	22.0	33.0	15.0	37.0
96-97	26.435579406042308	27.0	22.0	33.0	15.0	37.0
98-99	26.508741601462106	27.0	22.0	33.0	15.0	37.0
100	26.19538742712934	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.471173503414054
1101	2	11.362869456289893
1101	3	8.553496501112388
1101	4	12.867989189226375
1101	5	10.82383763776658
1101	6	10.201114412177098
1101	7	9.867556878968113
1101	8	9.413476404653313
1101	9	7.718980936921927
1101	10-11	8.039875314934264
1101	12-13	9.47950582967172
1101	14-15	8.271680680868478
1101	16-17	8.413174922201314
1101	18-19	8.478208890227087
1101	20-21	7.39803742539582
1101	22-23	7.564684647365343
1101	24-25	7.686915380450991
1101	26-27	8.759401720051947
1101	28-29	8.534629195392725
1101	30-31	5.834623648125973
1101	32-33	7.3698895847101795
1101	34-35	9.16223518806769
1101	36-37	8.519286937269765
1101	38-39	8.57306348358572
1101	40-41	9.439673982681565
1101	42-43	8.955978564247122
1101	44-45	8.908726260774106
1101	46-47	7.880393502349069
1101	48-49	5.618995985678545
1101	50-51	7.06218408430551
1101	52-53	6.110535869206956
1101	54-55	4.308825516359004
1101	56-57	6.599744569953266
1101	58-59	6.589571415074452
1101	60-61	7.256025149525069
1101	62-63	6.782010379019461
1101	64-65	7.055837210781224
1101	66-67	6.0867729589351995
1101	68-69	7.161054483913443
1101	70-71	6.70152939685558
1101	72-73	5.8984387294384355
1101	74-75	5.353301274029192
1101	76-77	5.460627961988688
1101	78-79	5.500784247071337
1101	80-81	5.276500718490986
1101	82-83	4.851024226210868
1101	84-85	4.670764989177631
1101	86-87	4.54433591044295
1101	88-89	4.509075249692266
1101	90-91	4.180525146679642
1101	92-93	4.1675916254048495
1101	94-95	4.066755099280957
1101	96-97	4.131235871648755
1101	98-99	3.868987557706564
1101	100	4.111109676199256
1102	1	6.697527952319174
1102	2	10.332562516264272
1102	3	7.998453940688826
1102	4	12.380689309093885
1102	5	10.446596575506952
1102	6	9.556955652258203
1102	7	6.6459460159168735
1102	8	7.730728250361043
1102	9	6.522915044243202
1102	10-11	7.55194021668764
1102	12-13	9.221996047690727
1102	14-15	6.304738685740311
1102	16-17	7.396304443908853
1102	18-19	7.531023886984855
1102	20-21	7.076370343915222
1102	22-23	6.606514976983004
1102	24-25	7.074892671445191
1102	26-27	8.564340624130686
1102	28-29	7.7303658895073895
1102	30-31	4.35956965375064
1102	32-33	4.535312769483561
1102	34-35	5.170321848525862
1102	36-37	5.17902299733619
1102	38-39	4.702535387741079
1102	40-41	6.2302217273365095
1102	42-43	6.934714486499335
1102	44-45	6.500311983015731
1102	46-47	5.568929930811404
1102	48-49	3.7189888892749323
1102	50-51	4.994378847673875
1102	52-53	5.818793768969737
1102	54-55	5.344861285318611
1102	56-57	6.380333630589973
1102	58-59	6.069526697447035
1102	60-61	6.077402242642741
1102	62-63	4.274458262877044
1102	64-65	6.222641584531313
1102	66-67	3.8808754104341077
1102	68-69	6.129560091872108
1102	70-71	5.416381205435751
1102	72-73	5.189488647495541
1102	74-75	4.348957940766137
1102	76-77	3.343566420742384
1102	78-79	4.436969670441236
1102	80-81	4.447161177690006
1102	82-83	3.9936860306411823
1102	84-85	3.0851288171628646
1102	86-87	3.299630226069386
1102	88-89	3.505241160523383
1102	90-91	3.3119146096515877
1102	92-93	3.300528310057647
1102	94-95	3.273808024567657
1102	96-97	3.481583441156232
1102	98-99	3.226351644864799
1102	100	3.333153908391523
1103	1	6.702705836179959
1103	2	10.902866980616245
1103	3	7.9627015602243
1103	4	11.994056030334416
1103	5	10.349639774231328
1103	6	9.29938305293345
1103	7	8.351649739614206
1103	8	8.296776347598392
1103	9	7.381170769080615
1103	10-11	7.764730727271754
1103	12-13	8.977511521120615
1103	14-15	8.76243271354737
1103	16-17	8.258580246147886
1103	18-19	7.497408818557382
1103	20-21	8.56972192107353
1103	22-23	7.0836394007887655
1103	24-25	5.400400533430464
1103	26-27	6.083204529005357
1103	28-29	7.193297628837406
1103	30-31	6.374966115446814
1103	32-33	8.287078145225578
1103	34-35	7.398817218941836
1103	36-37	4.359196070685169
1103	38-39	6.668102696487935
1103	40-41	7.067141633792577
1103	42-43	7.8411741008571845
1103	44-45	5.818460911207428
1103	46-47	6.62922990973053
1103	48-49	5.9172150089420725
1103	50-51	6.670833937195365
1103	52-53	6.397279781052173
1103	54-55	5.196309659825328
1103	56-57	6.231267657950458
1103	58-59	5.79485671825865
1103	60-61	5.236234375154115
1103	62-63	2.9099763894765793
1103	64-65	5.243691990157611
1103	66-67	4.392228152622852
1103	68-69	5.3890626094280165
1103	70-71	3.969481112377796
1103	72-73	4.687324541071913
1103	74-75	3.546164494620559
1103	76-77	3.814128452702569
1103	78-79	3.2311216642259915
1103	80-81	4.357946246649433
1103	82-83	3.5529822708239642
1103	84-85	3.6493201553520223
1103	86-87	3.165805136935365
1103	88-89	3.3325014936012494
1103	90-91	2.9856444210992237
1103	92-93	2.56981150111147
1103	94-95	3.127300324793506
1103	96-97	3.3184083194814775
1103	98-99	2.984733986313284
1103	100	3.1791329444109273
1104	1	6.215593572300303
1104	2	9.536235181088628
1104	3	7.101871695514692
1104	4	11.839754923510451
1104	5	9.299797855568514
1104	6	8.169551200448513
1104	7	3.904990405498843
1104	8	6.401254215541183
1104	9	6.568830535484185
1104	10-11	7.123983678047438
1104	12-13	7.387978228898877
1104	14-15	7.06283447625411
1104	16-17	6.9885124388085345
1104	18-19	6.6819361839014775
1104	20-21	7.4195564830648095
1104	22-23	6.37864814705382
1104	24-25	5.942906858961969
1104	26-27	8.226079706523585
1104	28-29	6.995014748334675
1104	30-31	6.298247595635932
1104	32-33	7.563442405791648
1104	34-35	8.03685364960809
1104	36-37	6.5632070110875524
1104	38-39	7.225700744571363
1104	40-41	6.916382168007534
1104	42-43	7.19726429927827
1104	44-45	6.638189025243609
1104	46-47	6.5624204780756195
1104	48-49	5.25310577054384
1104	50-51	5.4066442980153155
1104	52-53	5.723620510989431
1104	54-55	4.566132954987051
1104	56-57	5.781470643124287
1104	58-59	3.645427858612422
1104	60-61	4.083129966966855
1104	62-63	3.658097704608249
1104	64-65	2.4615332586461065
1104	66-67	3.0300494039967028
1104	68-69	3.843656482298833
1104	70-71	2.32158028299013
1104	72-73	2.2225826825220807
1104	74-75	3.047896381061417
1104	76-77	3.7346195567580693
1104	78-79	4.194199588395726
1104	80-81	2.847579195640229
1104	82-83	2.7304883966935307
1104	84-85	2.7691263385473484
1104	86-87	0.5088821606163414
1104	88-89	0.006620602535463149
1104	90-91	2.005280007235662
1104	92-93	2.2717441751047964
1104	94-95	2.3944498651556323
1104	96-97	2.7503477980609645
1104	98-99	2.2979037817763732
1104	100	0.5243011120261905
1105	1	5.721795426097863
1105	2	-2.686113479359502
1105	3	0.5411630069414564
1105	4	8.181038358837409
1105	5	8.467164174414208
1105	6	8.125686350685253
1105	7	5.147669776587193
1105	8	5.881793939583606
1105	9	4.780166932636384
1105	10-11	4.933498493189013
1105	12-13	5.0372511199835905
1105	14-15	5.8191403287897785
1105	16-17	5.993222940007591
1105	18-19	6.194206083351538
1105	20-21	7.131995560924842
1105	22-23	2.019816274782446
1105	24-25	3.943573252932719
1105	26-27	6.857128436215092
1105	28-29	5.990249146518849
1105	30-31	5.365429470447115
1105	32-33	7.136983923745461
1105	34-35	6.0486436844436575
1105	36-37	6.160265504362389
1105	38-39	3.9858548979708317
1105	40-41	5.140646116270165
1105	42-43	5.470584719657751
1105	44-45	6.046099212040314
1105	46-47	5.344787585600688
1105	48-49	4.8857460284820995
1105	50-51	5.786992921034592
1105	52-53	5.504190151378367
1105	54-55	5.228891407892199
1105	56-57	5.165087205925889
1105	58-59	1.7796822892527473
1105	60-61	4.286458995982233
1105	62-63	-0.8938757583358523
1105	64-65	-0.48613208999028146
1105	66-67	0.5588735498836748
1105	68-69	2.5308263798774533
1105	70-71	2.6647487689663407
1105	72-73	2.480806362687197
1105	74-75	2.6161644946205556
1105	76-77	1.6711666382433492
1105	78-79	3.1434545403664877
1105	80-81	1.5561661762814936
1105	82-83	0.8788731011259081
1105	84-85	1.3409586947203032
1105	86-87	-0.506713399252444
1105	88-89	1.5001211091617073
1105	90-91	1.3284321047217809
1105	92-93	1.6749600887831697
1105	94-95	0.9971114842742921
1105	96-97	1.9299182485171968
1105	98-99	1.7860256554646945
1105	100	1.9954523433017393
1106	1	5.900131031483092
1106	2	10.10515965592553
1106	3	6.449088616897441
1106	4	11.472961074425815
1106	5	9.684509991373446
1106	6	9.360323668571297
1106	7	6.499485725725215
1106	8	7.249044516924652
1106	9	6.829664482684823
1106	10-11	5.527519998393416
1106	12-13	7.836637892510556
1106	14-15	7.70136007685187
1106	16-17	7.659161710541007
1106	18-19	7.090876467921312
1106	20-21	8.121370087025461
1106	22-23	6.090380543201729
1106	24-25	4.887124943112788
1106	26-27	7.627241145980115
1106	28-29	6.068464952804099
1106	30-31	5.076617347151316
1106	32-33	7.250315884519587
1106	34-35	6.5221662443865185
1106	36-37	5.836717585388687
1106	38-39	6.991749253554282
1106	40-41	6.461423584444784
1106	42-43	5.916519559074988
1106	44-45	4.159233804130377
1106	46-47	5.654306563563782
1106	48-49	5.67909190140977
1106	50-51	6.350543891620514
1106	52-53	5.247288430615544
1106	54-55	4.913477954413292
1106	56-57	4.419174913791984
1106	58-59	4.705444852500413
1106	60-61	4.7380322420048095
1106	62-63	4.735682384205088
1106	64-65	4.911437334187816
1106	66-67	2.8047719698414824
1106	68-69	3.1470934852300374
1106	70-71	2.631755417216727
1106	72-73	2.5617325736375527
1106	74-75	2.5661229713730016
1106	76-77	3.243045192072323
1106	78-79	3.872417578546287
1106	80-81	3.3072082552156417
1106	82-83	1.6696322996046327
1106	84-85	2.3637591152831057
1106	86-87	3.1381935532263547
1106	88-89	3.210933864101257
1106	90-91	2.9351018363264885
1106	92-93	2.6845460289795646
1106	94-95	1.1557763839375461
1106	96-97	1.2784779675023543
1106	98-99	1.7018504369546008
1106	100	2.6542967284018744
1107	1	5.894297271429835
1107	2	10.150826137130064
1107	3	5.818668065009671
1107	4	11.06596595279003
1107	5	8.89824062691616
1107	6	7.809818301214424
1107	7	3.439579681904405
1107	8	6.066312161904953
1107	9	5.680200918606548
1107	10-11	4.3939693581850605
1107	12-13	5.950204925615704
1107	14-15	5.830500750003395
1107	16-17	6.755297225903945
1107	18-19	6.09437882766553
1107	20-21	5.212511908707526
1107	22-23	5.560600368286348
1107	24-25	4.198156526525089
1107	26-27	6.898266010360267
1107	28-29	6.1316301982784225
1107	30-31	4.7989009514388705
1107	32-33	4.545899983709461
1107	34-35	6.36977562966252
1107	36-37	6.318195121542299
1107	38-39	6.055170664805836
1107	40-41	7.506858831271256
1107	42-43	5.947671723741376
1107	44-45	3.8410797763136273
1107	46-47	3.109792975563142
1107	48-49	3.670218236986024
1107	50-51	4.561747770673225
1107	52-53	3.647214610656274
1107	54-55	5.018145548604739
1107	56-57	5.024720821236567
1107	58-59	2.8740484243576034
1107	60-61	2.421633944073971
1107	62-63	0.06917623488819657
1107	64-65	1.7670166039395063
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2206	30-31	6.303094928498933
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2206	78-79	0.9977010713776977
2206	80-81	1.9289838999908184
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2206	86-87	-1.6656844915628817
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2206	96-97	1.422180009396378
2206	98-99	1.2557209920606276
2206	100	1.5394068254135789
2207	1	6.230153615309433
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2207	3	7.706609976217525
2207	4	11.873753985896055
2207	5	10.347421807774793
2207	6	9.18501509646213
2207	7	5.663318635386229
2207	8	6.770964872360295
2207	9	6.110545869032318
2207	10-11	6.976175996436552
2207	12-13	8.887326944301872
2207	14-15	8.324042109047419
2207	16-17	7.411867785356144
2207	18-19	4.232465060702133
2207	20-21	7.531250413707674
2207	22-23	6.385151121427366
2207	24-25	4.343297100024753
2207	26-27	3.8267065609257216
2207	28-29	5.070722188671983
2207	30-31	5.628842305551711
2207	32-33	6.8842104363294965
2207	34-35	6.249264777149868
2207	36-37	6.738807169375523
2207	38-39	6.801782449501474
2207	40-41	3.9727179244923185
2207	42-43	5.60537985377896
2207	44-45	3.9426851334803956
2207	46-47	5.528763229750229
2207	48-49	2.4198359382389896
2207	50-51	5.46664319261053
2207	52-53	2.816804529425827
2207	54-55	3.059729285128764
2207	56-57	5.271032192287564
2207	58-59	3.563898370040306
2207	60-61	3.4806393604998362
2207	62-63	4.335059394146164
2207	64-65	3.493555326506403
2207	66-67	3.537857408775306
2207	68-69	3.45402973244466
2207	70-71	3.557460903767481
2207	72-73	3.8844451098133597
2207	74-75	3.500893857892059
2207	76-77	4.163815330366461
2207	78-79	4.267501087926007
2207	80-81	4.373745988916589
2207	82-83	3.988365877179607
2207	84-85	3.1585168923979516
2207	86-87	3.3517919145348323
2207	88-89	3.3188174388519798
2207	90-91	1.229310619136605
2207	92-93	-0.9762368560206447
2207	94-95	1.131083001210417
2207	96-97	-1.0744906207586098
2207	98-99	0.636207914267434
2207	100	1.9742951890683038
2208	1	0.9371733995391587
2208	2	8.241506915941951
2208	3	6.353651362123152
2208	4	11.655951194670035
2208	5	9.837571595652001
2208	6	9.088784822282378
2208	7	7.5975979095510375
2208	8	7.880929700804234
2208	9	6.500836075669472
2208	10-11	6.746783085341946
2208	12-13	6.84310805413714
2208	14-15	7.894666163580759
2208	16-17	7.0353858298879
2208	18-19	6.464061759668176
2208	20-21	6.522220464785484
2208	22-23	5.7531708996260384
2208	24-25	6.109783888205875
2208	26-27	8.013091631382899
2208	28-29	7.031735752521087
2208	30-31	6.081003980906164
2208	32-33	8.430558539024254
2208	34-35	6.4600881660117615
2208	36-37	6.847032666957464
2208	38-39	7.302696548095632
2208	40-41	7.6087068200037145
2208	42-43	6.988461464742688
2208	44-45	5.739071048285673
2208	46-47	4.4263835972094725
2208	48-49	4.623501379202409
2208	50-51	5.955378041872102
2208	52-53	5.560453720621634
2208	54-55	6.374998271619983
2208	56-57	6.563572953716488
2208	58-59	5.777826455706503
2208	60-61	5.236225772054507
2208	62-63	3.2951673684370775
2208	64-65	3.230941342790782
2208	66-67	2.7677408415380533
2208	68-69	5.331574596304019
2208	70-71	5.262553647015121
2208	72-73	4.519706213974185
2208	74-75	1.9243809866968355
2208	76-77	3.2202787495095073
2208	78-79	4.16283590734696
2208	80-81	3.537088655530461
2208	82-83	1.3561356680303867
2208	84-85	1.917683208782524
2208	86-87	1.1070436023788126
2208	88-89	0.6980695247457831
2208	90-91	1.8864512736322467
2208	92-93	0.8366282294774656
2208	94-95	-1.2797777933211414
2208	96-97	1.2955238118250456
2208	98-99	0.25818081488896993
2208	100	1.761840451501918
2209	1	5.3028222194752
2209	2	9.520142626003853
2209	3	5.423385437227179
2209	4	-3.1862794945714548
2209	5	0.6785977839518402
2209	6	4.103344856061092
2209	7	2.232276926689405
2209	8	5.01502552986496
2209	9	5.172284967060488
2209	10-11	5.081123838444391
2209	12-13	7.082007451616004
2209	14-15	6.559627899596386
2209	16-17	6.241499315957533
2209	18-19	5.270531785955978
2209	20-21	6.021444010591683
2209	22-23	5.496564008190685
2209	24-25	5.260204652835974
2209	26-27	6.898404437650733
2209	28-29	6.241692431009277
2209	30-31	4.043556508819226
2209	32-33	6.250414708821069
2209	34-35	5.564248368993383
2209	36-37	6.216441869388998
2209	38-39	6.813012022325189
2209	40-41	7.279795188567032
2209	42-43	6.740618660016349
2209	44-45	6.527243587303648
2209	46-47	6.831917323596723
2209	48-49	5.173986123359057
2209	50-51	4.629030746979073
2209	52-53	4.888369106795562
2209	54-55	5.4032108841963655
2209	56-57	5.14892980068581
2209	58-59	3.1646875948720243
2209	60-61	4.065245101501635
2209	62-63	4.330067955285543
2209	64-65	5.1100289823690055
2209	66-67	4.53891396621874
2209	68-69	3.8256601891560464
2209	70-71	3.689057165889082
2209	72-73	0.8029872062806582
2209	74-75	2.2734837524580485
2209	76-77	3.4562944412488292
2209	78-79	3.586321291298873
2209	80-81	3.801357958813526
2209	82-83	3.1458618500920927
2209	84-85	3.091370816499868
2209	86-87	1.6002878335237973
2209	88-89	2.0778490557310008
2209	90-91	2.4105844696235508
2209	92-93	2.4102909877735392
2209	94-95	0.636040139299805
2209	96-97	0.11297506545772507
2209	98-99	0.17068430128339784
2209	100	0.027252196616970537
2210	1	1.4150182268651967
2210	2	-16.779121609440804
2210	3	-7.430979817576677
2210	4	-9.222168684188079
2210	5	-2.7113767500399817
2210	6	1.7123868165663154
2210	7	-9.999095775618889
2210	8	-10.039784346704876
2210	9	-4.675720525114599
2210	10-11	-1.9282895537644862
2210	12-13	2.939052298388635
2210	14-15	-6.737842977138786
2210	16-17	-2.5008366196891316
2210	18-19	-0.8977116795058606
2210	20-21	1.6755097657298137
2210	22-23	2.1118974385716136
2210	24-25	1.409150922613911
2210	26-27	3.5493098557820986
2210	28-29	2.9623337359040676
2210	30-31	2.3267160101383517
2210	32-33	4.157225428267253
2210	34-35	1.8502804342381225
2210	36-37	3.577088035649499
2210	38-39	2.4168704730142245
2210	40-41	3.5211610975435725
2210	42-43	1.573568071260926
2210	44-45	2.7625238032525203
2210	46-47	2.398888252450053
2210	48-49	2.5329339566816067
2210	50-51	1.9425528918028405
2210	52-53	1.9633967833323247
2210	54-55	2.35950801518187
2210	56-57	2.326659668701076
2210	58-59	1.2737387659130235
2210	60-61	2.2341575663668145
2210	62-63	1.234785861285964
2210	64-65	2.2937919759638596
2210	66-67	2.4702586325547244
2210	68-69	2.7417731004401666
2210	70-71	1.8578251825956436
2210	72-73	0.5643330138244913
2210	74-75	0.02069632982654568
2210	76-77	-0.4629729662144939
2210	78-79	-0.12705267622253302
2210	80-81	0.4379488564678482
2210	82-83	0.3555354287042398
2210	84-85	-1.9727457448521868
2210	86-87	-1.4389623030617074
2210	88-89	-1.6821733550750686
2210	90-91	-2.231072896648456
2210	92-93	-1.5648902126692867
2210	94-95	-0.822034400442984
2210	96-97	-0.2915235848618103
2210	98-99	-0.5583408376384362
2210	100	-0.4080970309560499
2211	1	0.7791091874089311
2211	2	-16.779121609440804
2211	3	-7.876515272600868
2211	4	-18.67652261677235
2211	5	-20.798689484122377
2211	6	-13.45520486006654
2211	7	-11.71526431740552
2211	8	-18.511275940655274
2211	9	-15.875781060483497
2211	10-11	-10.56571520722657
2211	12-13	-22.22925294505706
2211	14-15	-17.730283407628924
2211	16-17	-15.636250794808603
2211	18-19	-18.95303193252402
2211	20-21	-9.033039747772527
2211	22-23	-4.6859009565028735
2211	24-25	-2.472985083872725
2211	26-27	-17.250218657360968
2211	28-29	-4.3606537091588535
2211	30-31	-5.465763546870708
2211	32-33	-1.4066117943404919
2211	34-35	-6.361118609499911
2211	36-37	-7.4804351142075305
2211	38-39	-3.1330829565851133
2211	40-41	-6.194618885919933
2211	42-43	-2.3769943109288754
2211	44-45	-0.6579391200644231
2211	46-47	0.2999488517994173
2211	48-49	0.021678530645491634
2211	50-51	-5.2906230741792335
2211	52-53	-1.6811197211684537
2211	54-55	-1.186391328772224
2211	56-57	-7.375429016844748
2211	58-59	-2.7694099406971446
2211	60-61	-1.7658892193527471
2211	62-63	-0.799978020015498
2211	64-65	-4.147299573938444
2211	66-67	-3.1516653217336916
2211	68-69	-0.3752641300420976
2211	70-71	-0.6546605448377072
2211	72-73	-1.687231093377246
2211	74-75	-2.3026309499698954
2211	76-77	-2.0653490717232117
2211	78-79	-1.4584350358223084
2211	80-81	-1.3191449782755171
2211	82-83	-1.8082556233807843
2211	84-85	-1.957809934490303
2211	86-87	-2.3482226457509547
2211	88-89	-3.002019420522167
2211	90-91	-2.6798152805115976
2211	92-93	-2.4038054422361377
2211	94-95	-2.5477589792792266
2211	96-97	-3.4455255940946863
2211	98-99	-3.16632117102591
2211	100	-2.7319284030418594
2212	1	-20.870562297479374
2212	2	-16.779121609440804
2212	3	-19.668105285741472
2212	4	-18.67652261677235
2212	5	-20.798689484122377
2212	6	-21.78293153549361
2212	7	-22.146801768128253
2212	8	-22.579344271798508
2212	9	-24.09706883972134
2212	10-11	-11.255931106520993
2212	12-13	-22.22925294505706
2212	14-15	-23.58744503706388
2212	16-17	-24.00458193804119
2212	18-19	-18.639014102035027
2212	20-21	-23.698085739888164
2212	22-23	-18.711814660953664
2212	24-25	-25.23211780397785
2212	26-27	-17.24812090353945
2212	28-29	-18.108544028355578
2212	30-31	-6.239676168825838
2212	32-33	-17.274854147819966
2212	34-35	-13.833319186458937
2212	36-37	-17.656476249042406
2212	38-39	-23.686828028858436
2212	40-41	-10.275319913453824
2212	42-43	-14.156470067769105
2212	44-45	-17.153179696353863
2212	46-47	-17.054331051445555
2212	48-49	-17.647328126144796
2212	50-51	-9.054301160059083
2212	52-53	-2.2940968353131552
2212	54-55	-23.47850696609154
2212	56-57	-23.36938433878956
2212	58-59	-12.655852380106683
2212	60-61	-6.49709571376869
2212	62-63	-3.320200391964356
2212	64-65	-17.303182993620336
2212	66-67	-17.55163030802115
2212	68-69	-14.599309553010537
2212	70-71	-9.890312595023097
2212	72-73	-17.506041547830126
2212	74-75	-6.4459268004962205
2212	76-77	-11.307168766448276
2212	78-79	-4.994936273159482
2212	80-81	-2.5140269778793467
2212	82-83	-2.973550129157349
2212	84-85	-5.511044308749224
2212	86-87	-5.043599370978402
2212	88-89	-3.5348043219397383
2212	90-91	-4.392105930594877
2212	92-93	-5.543046746699201
2212	94-95	-3.7118546490176136
2212	96-97	-5.693412495618269
2212	98-99	-3.6992166934556074
2212	100	-3.850614147299474
2213	1	-20.870562297479374
2213	2	-16.779121609440804
2213	3	-19.668105285741472
2213	4	-18.67652261677235
2213	5	-20.798689484122377
2213	6	-21.78293153549361
2213	7	-10.522484828237543
2213	8	-10.221420774530749
2213	9	-4.339782865222251
2213	10-11	-17.62340874391614
2213	12-13	-16.27979939314449
2213	14-15	-23.58744503706388
2213	16-17	-17.951758622922796
2213	18-19	-18.5481352023564
2213	20-21	-17.732238745352646
2213	22-23	-19.82195701590128
2213	24-25	-7.501243487037961
2213	26-27	-23.274660181671088
2213	28-29	-24.156580121773835
2213	30-31	-19.45696179526882
2213	32-33	-23.35155266162039
2213	34-35	-17.876430285968176
2213	36-37	-23.769002788320538
2213	38-39	-11.496481945069728
2213	40-41	-17.158042408319996
2213	42-43	-23.267138857578022
2213	44-45	-17.122376730915835
2213	46-47	-16.92258746437932
2213	48-49	-6.07536752609607
2213	50-51	-23.37064935538817
2213	52-53	-17.752665106518474
2213	54-55	-17.71279658357788
2213	56-57	-17.460231333325076
2213	58-59	-17.998926933724725
2213	60-61	-19.31984959169381
2213	62-63	-17.459431536024777
2213	64-65	-17.604399449713057
2213	66-67	-8.779607057873225
2213	68-69	-22.72112483963474
2213	70-71	-17.406162199979413
2213	72-73	-9.358124953675148
2213	74-75	-4.318316379696384
2213	76-77	-3.1130114682918126
2213	78-79	-4.7108963859296615
2213	80-81	-3.4594053728766205
2213	82-83	-3.0708028062888992
2213	84-85	-3.101783985985197
2213	86-87	-3.2661116399561614
2213	88-89	-2.8953686949166624
2213	90-91	-2.6617058401002893
2213	92-93	-3.813074475169028
2213	94-95	-3.6911989693220484
2213	96-97	-3.337748462576144
2213	98-99	-3.716647053229604
2213	100	-3.152317881941496
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	742.0
3	698.0
4	3035.0
5	11761.0
6	19111.0
7	21953.0
8	20011.0
9	20011.0
10	23763.0
11	29826.0
12	39570.0
13	51046.0
14	43161.0
15	47649.0
16	54681.0
17	58111.0
18	58831.0
19	58491.0
20	59176.0
21	58235.0
22	61733.0
23	67339.0
24	73925.0
25	81606.0
26	88040.0
27	94161.0
28	101419.0
29	106553.0
30	112877.0
31	124879.0
32	143111.0
33	170233.0
34	200085.0
35	225267.0
36	227964.0
37	176228.0
38	44751.0
39	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.10592966444535	21.03532125701414	13.943862091525054	23.914886987015464
2	15.678884021862387	21.4393383995156	39.45411153447467	23.42766604414735
3	18.38442682091576	26.266087317313513	29.68479002018113	25.664695841589598
4	12.489413398449585	18.41361729767867	39.07703032935938	30.01993897451236
5	13.83181673687531	37.41416733284283	35.46346477713398	13.29055115314788
6	30.378228489938202	37.97860877832356	18.92069402630328	12.722468705434956
7	26.75989106415081	31.518567140543063	23.51718303646405	18.204358758842076
8	24.47258674660712	35.11893416913147	21.816951109416927	18.591527974844485
9	24.668624509040026	17.212017813764568	21.780649178914548	36.33870849828086
10-11	23.350834750634068	26.955803052542436	29.26258592795475	20.430776268868744
12-13	24.4504724139132	24.517938792670247	28.995238545393033	22.036350248023524
14-15	21.69166355670146	26.265433061121808	26.386068078897512	25.656835303279223
16-17	21.537244207418635	29.73934063305873	26.92720438014584	21.796210779376786
18-19	21.401912569302002	28.852579290200918	28.472083790358337	21.273424350138743
20-21	22.826958300902774	28.176350004583	27.514824843906084	21.481866850608142
22-23	21.97926713178992	28.094743560310214	28.106327016931402	21.819662290968456
24-25	22.18400837493534	28.097056951640802	27.60182364784418	22.11711102557968
26-27	22.06262588083258	28.102062140081287	27.665198011697502	22.170113967388634
28-29	21.935162740646188	27.958630429455305	28.063051387832278	22.04315544206623
30-31	22.43695919872759	28.321546614956226	27.439974144780255	21.801520041535934
32-33	21.756446306741204	28.14811141816796	27.97606544345421	22.119376831636625
34-35	21.937427444255512	27.806511592059692	27.76556727151865	22.490493692166147
36-37	22.391013286142265	28.264235880468952	27.33449823783514	22.010252595553645
38-39	22.082624020405355	28.110123712808928	27.589114400350756	22.218137866434965
40-41	22.315971183155384	27.92490856103733	27.338819735950658	22.420300519856625
42-43	22.02359292735558	28.06071118787335	27.736235373804274	22.179460510966795
44-45	22.341596029263602	27.677244429275362	27.698127369135168	22.283032172325868
46-47	22.279878909579434	27.977215432297164	27.650824983005435	22.092080675117966
48-49	22.167614073166252	27.806020011971956	27.693782943398933	22.332582971462863
50-51	22.49230298217583	27.732245194745552	27.474670161977023	22.300781661101595
52-53	22.086901798864574	27.76314440635708	27.65216191898878	22.49779187578957
54-55	22.399914107147566	27.841094455767063	27.458984279347664	22.300007157737703
56-57	22.415910915501975	27.767171404514773	27.424842862500736	22.392074817482516
58-59	22.15878480956241	27.392984186144982	27.739141496443253	22.70908950784936
60-61	22.48818724793956	27.775413422989665	27.36058115342933	22.375818175641445
62-63	22.171884660159463	27.82268413854208	27.628881851663994	22.376549349634463
64-65	22.293336056415864	27.316240734511467	27.64800379029061	22.742419418782063
66-67	22.202026107132287	27.635018162707027	27.42819857991122	22.734757150249468
68-69	22.677484365977648	27.50763302599946	27.391750670838256	22.423131937184632
70-71	22.739161221376236	27.478555345852822	27.270579868010536	22.5117035647604
72-73	22.388423205220167	27.66909526486338	27.59002448437398	22.35245704554247
74-75	22.523172330973974	27.403430653680953	27.31617317999499	22.757223835350075
76-77	22.567280377372334	27.668286754447553	27.221506474600123	22.542926393579993
78-79	22.57635629963778	27.48310085734062	27.441070955493768	22.499471887527836
80-81	22.667857885230223	27.358024356435863	27.319347635532576	22.654770122801338
82-83	22.26833794138343	27.50966966031441	27.734424171841475	22.487568226460684
84-85	22.438399036117055	27.244008153861856	27.366530714401478	22.95106209561962
86-87	22.58566803806794	27.475618864086815	27.383616281152673	22.555096816692576
88-89	22.35227804033305	27.11226668013382	27.668981605925985	22.86647367360714
90-91	22.521234709404293	27.155813050163	27.439669838183057	22.883282402249648
92-93	22.29131977344998	27.392591398076394	27.58599479459502	22.730094033878604
94-95	22.262902091771906	26.97904974668467	27.582234850333386	23.17581331121004
96-97	22.326648735023824	27.167378871029552	27.63173973088075	22.87423266306588
98-99	22.243789626341563	27.013465707616696	27.660602241809723	23.082142424232025
100	22.456957227937195	26.83140227395777	27.373362208987544	23.338278289117486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	245.0
1	305.0
2	299.5
3	232.5
4	273.0
5	361.0
6	495.0
7	684.5
8	1016.5
9	1510.0
10	2114.5
11	2861.0
12	3692.5
13	4561.5
14	5377.0
15	6144.0
16	6801.0
17	7392.5
18	8068.0
19	8667.0
20	9343.0
21	10143.0
22	11025.0
23	12068.0
24	13142.0
25	14560.0
26	16617.5
27	19239.0
28	23013.5
29	28319.5
30	35131.5
31	43514.0
32	53334.0
33	63870.0
34	74187.0
35	84006.0
36	93014.5
37	100818.5
38	107162.0
39	111294.5
40	113898.0
41	114614.0
42	113287.5
43	111179.5
44	107927.0
45	104054.0
46	99990.0
47	95464.0
48	90680.5
49	86403.5
50	82964.5
51	78294.0
52	72286.0
53	66733.0
54	61568.5
55	56703.5
56	51613.0
57	46537.5
58	41673.5
59	36749.0
60	31891.5
61	26881.5
62	22200.5
63	18054.5
64	14453.5
65	11561.0
66	9151.5
67	7111.0
68	5408.5
69	4065.5
70	3066.0
71	2319.0
72	1758.5
73	1341.0
74	985.0
75	732.0
76	515.5
77	350.0
78	255.0
79	179.0
80	131.0
81	89.0
82	59.0
83	41.5
84	24.5
85	17.5
86	17.0
87	13.0
88	6.5
89	4.5
90	3.0
91	1.5
92	0.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	7.192231062441347
2	23.902043705669264
3	5.677679591755251
4	22.914955845065837
5	11.853687119550145
6	9.19741289602485
7	7.6909546681284855
8	8.280250653124693
9	5.039968029892482
10-11	6.276705453083112
12-13	9.630401385275405
14-15	7.134373368986766
16-17	7.354959197466851
18-19	6.259547778368884
20-21	7.386972468946228
22-23	5.288718343280828
24-25	4.909702790945784
26-27	8.927332391639572
28-29	5.838914973110651
30-31	3.8397581091595003
32-33	7.872495096394119
34-35	6.658382783046779
36-37	6.48070912633624
38-39	5.571891916001492
40-41	7.566282759198689
42-43	5.84477807788721
44-45	5.781596829175385
46-47	5.151780675278596
48-49	3.6150177493806868
50-51	6.009574486070091
52-53	4.032539152410869
54-55	4.518799164201825
56-57	4.476858181567047
58-59	3.153828805253917
60-61	3.6482180298025573
62-63	2.8098480017064156
64-65	4.188666798316749
66-67	2.231127187288789
68-69	4.6914550103827315
70-71	2.2383571508476146
72-73	1.489570327941795
74-75	0.7355139047306624
76-77	0.9695345162416994
78-79	1.4952895528465373
80-81	1.8833155821541478
82-83	0.7024754891645867
84-85	1.1228205346791857
86-87	0.4011730525949274
88-89	0.6410387838985474
90-91	0.26603388299041364
92-93	0.293892623171062
94-95	0.49573809834692817
96-97	0.5049104401752889
98-99	0.44201695120709816
100	0.3452037824579502
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2780097.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.77502345082551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.13458365355537	93.03067027305437
2	2.2607358691149204	4.330440617612078
3	0.3544009624336163	1.0182828146422418
4	0.11363644846278553	0.43534134065567237
5	0.050524801193405536	0.24195070095733556
6	0.025223554181500137	0.1449471895947806
7	0.016546206382254	0.11092993129978031
8	0.009872401174140585	0.07564235631754174
9	0.005997917008284206	0.051700557791207254
>10	0.027534616513486362	0.4155314127249208
>50	4.5266965314829776E-4	0.030923014986040404
>100	4.5324441340285107E-4	0.09153196975055047
>500	3.7655913742668936E-5	0.02210782061342791
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.19399359087111E-5	0.0
4	0.0	0.0	0.0	7.19399359087111E-5	0.0
5	0.0	0.0	0.0	7.19399359087111E-5	0.0
6	0.0	0.0	0.0	7.19399359087111E-5	0.0
7	0.0	0.0	0.0	7.19399359087111E-5	0.0
8	0.0	0.0	0.0	7.19399359087111E-5	0.0
9	0.0	0.0	0.0	1.0790990386306665E-4	0.0
10-11	0.0	0.0	0.0	1.2589488784024442E-4	0.0
12-13	0.0	0.0	0.0	1.438798718174222E-4	0.0
14-15	0.0	0.0	0.0	1.438798718174222E-4	0.0
16-17	0.0	0.0	0.0	1.438798718174222E-4	0.0
18-19	0.0	0.0	0.0	1.438798718174222E-4	0.0
20-21	0.0	0.0	0.0	2.338047917033111E-4	0.0
22-23	3.596996795435555E-5	0.0	0.0	2.697747596576666E-4	0.0
24-25	7.19399359087111E-5	0.0	0.0	3.4171469556637773E-4	0.0
26-27	7.19399359087111E-5	0.0	0.0	4.4962459942944437E-4	0.0
28-29	7.19399359087111E-5	0.0	0.0	7.014143751099332E-4	0.0
30-31	7.19399359087111E-5	0.0	0.0	0.0016905884938547108	1.7984983977177776E-5
32-33	7.19399359087111E-5	0.0	0.0	0.005611315000879465	3.596996795435555E-5
34-35	7.19399359087111E-5	0.0	0.0	0.011168675049827399	3.596996795435555E-5
36-37	7.19399359087111E-5	1.7984983977177776E-5	0.0	0.01989139227875862	3.596996795435555E-5
38-39	7.19399359087111E-5	3.596996795435555E-5	0.0	0.034531169236181325	3.596996795435555E-5
40-41	7.19399359087111E-5	3.596996795435555E-5	0.0	0.0534873423481267	3.596996795435555E-5
42-43	7.19399359087111E-5	3.596996795435555E-5	0.0	0.07377440427438323	3.596996795435555E-5
44-45	7.19399359087111E-5	7.19399359087111E-5	0.0	0.09837786235516242	3.596996795435555E-5
46-47	8.992491988588889E-5	7.19399359087111E-5	0.0	0.12726174662250994	3.596996795435555E-5
48-49	1.7984983977177774E-4	7.19399359087111E-5	0.0	0.15815994909530134	3.596996795435555E-5
50-51	1.7984983977177774E-4	7.19399359087111E-5	0.0	0.19465148158499507	3.596996795435555E-5
52-53	1.7984983977177774E-4	7.19399359087111E-5	0.0	0.23364292684751647	3.596996795435555E-5
54-55	1.7984983977177774E-4	7.19399359087111E-5	0.0	0.2795046359893198	3.596996795435555E-5
56-57	1.7984983977177774E-4	8.992491988588889E-5	0.0	0.3320927291385876	3.596996795435555E-5
58-59	1.7984983977177774E-4	1.0790990386306665E-4	0.0	0.3913532513433884	3.596996795435555E-5
60-61	1.7984983977177774E-4	1.0790990386306665E-4	0.0	0.458796941257805	3.596996795435555E-5
62-63	2.158198077261333E-4	1.0790990386306665E-4	0.0	0.5269780155152859	3.596996795435555E-5
64-65	2.158198077261333E-4	1.0790990386306665E-4	0.0	0.6012200293730758	3.596996795435555E-5
66-67	2.158198077261333E-4	1.0790990386306665E-4	0.0	0.6944721712947426	3.596996795435555E-5
68-69	2.158198077261333E-4	1.0790990386306665E-4	0.0	0.7976879943397659	3.596996795435555E-5
70-71	2.158198077261333E-4	1.0790990386306665E-4	0.0	0.9144824802875583	3.596996795435555E-5
72-73	2.158198077261333E-4	1.0790990386306665E-4	0.0	1.0415463920863193	3.596996795435555E-5
74-75	2.158198077261333E-4	1.0790990386306665E-4	0.0	1.1761100422035633	3.596996795435555E-5
76-77	2.158198077261333E-4	1.0790990386306665E-4	0.0	1.3242523552235768	3.596996795435555E-5
78-79	2.158198077261333E-4	1.2589488784024442E-4	0.0	1.4996778889369686	3.596996795435555E-5
80-81	2.338047917033111E-4	1.438798718174222E-4	0.0	1.6980342772212624	3.596996795435555E-5
82-83	2.5178977568048885E-4	1.438798718174222E-4	0.0	1.914375649482734	5.395495193153333E-5
84-85	2.5178977568048885E-4	1.438798718174222E-4	0.0	2.1352132677385	7.19399359087111E-5
86-87	2.5178977568048885E-4	1.438798718174222E-4	0.0	2.380582404139136	8.992491988588889E-5
88	2.5178977568048885E-4	1.438798718174222E-4	0.0	2.583183248642044	1.0790990386306665E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	5715	0.0	20.994234	1
GTATTAG	1075	0.0	20.50002	1
GTATCAA	3455	0.0	19.489685	1
TACATGG	6000	0.0	19.37882	2
GTATAAT	1240	0.0	19.25321	1
ACATGGG	6350	0.0	18.744904	3
GTATAGG	820	0.0	17.916683	1
ATCAACG	3470	0.0	17.792488	3
GTATTAC	870	0.0	17.590614	1
TCAACGC	3495	0.0	17.50731	4
TATCAAC	3800	0.0	17.231583	2
CAACGCA	3505	0.0	16.73079	5
AACGCAG	3650	0.0	16.248343	6
GTATAAG	835	0.0	16.12859	1
TCTGTCG	655	0.0	16.12789	8
CATGGGG	3875	0.0	15.934013	4
TATACAG	1435	0.0	15.717346	5
GTATTGG	1105	0.0	15.511578	1
GTGTATA	1305	0.0	15.47974	1
CAACGCG	200	0.003695639	15.036261	5
>>END_MODULE