FastQCFastQC Report
Wed 25 May 2016
SRR1295108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780097
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51300.024.5651281
TCAACGC55450.021.9677964
ATCAACG56100.021.7963163
CAACGCA56300.021.6361315
AACGCAG58400.020.9092626
TATCAAC60400.020.2779122
GTACATG78950.018.403121
ACGCAGA66700.018.162947
CGCAGAG68150.017.7075928
TACATGG80450.017.6183152
GTATTAG12350.017.6068611
GGTATCA36750.017.347171
TATACCG2203.1788855E-517.102345
ACATGGG82200.016.706433
TACACCG2053.5956566E-416.0595155
ATACCGT2951.3861918E-615.9204816
TCTATAC12250.014.9727343
GATACCG1900.004011650614.8520345
GCAGAGT80400.014.6589239
CATGGGG45750.014.4949364
Produced by FastQC (version 0.11.5)