##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295108_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2780097 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.5173999324484 33.0 33.0 33.0 27.0 33.0 2 30.317609061842088 33.0 33.0 33.0 27.0 33.0 3 31.074457114266156 33.0 33.0 33.0 27.0 33.0 4 35.303739042198885 37.0 37.0 37.0 33.0 37.0 5 35.60457782588161 37.0 37.0 37.0 33.0 37.0 6 35.418086491226745 37.0 37.0 37.0 33.0 37.0 7 35.31491455154263 37.0 37.0 37.0 33.0 37.0 8 35.30529941940875 37.0 37.0 37.0 33.0 37.0 9 35.264568826195635 37.0 37.0 37.0 33.0 37.0 10-11 35.25039773792065 37.0 37.0 37.0 33.0 37.0 12-13 35.17505648184218 37.0 37.0 37.0 33.0 37.0 14-15 36.68047751571258 40.0 37.0 40.0 33.0 40.0 16-17 36.675473733470454 40.0 37.0 40.0 33.0 40.0 18-19 36.68367866301068 40.0 37.0 40.0 33.0 40.0 20-21 36.676744732288114 40.0 37.0 40.0 33.0 40.0 22-23 36.63690439578187 40.0 37.0 40.0 33.0 40.0 24-25 36.61156103546027 40.0 37.0 40.0 33.0 40.0 26-27 36.59671065434048 40.0 37.0 40.0 33.0 40.0 28-29 36.54540578979798 40.0 37.0 40.0 33.0 40.0 30-31 36.465744900267865 40.0 37.0 40.0 33.0 40.0 32-33 36.432634185066206 40.0 37.0 40.0 33.0 40.0 34-35 36.33653627912983 37.0 37.0 40.0 33.0 40.0 36-37 36.33612262449836 37.0 37.0 40.0 33.0 40.0 38-39 36.27373289493136 37.0 37.0 40.0 33.0 40.0 40-41 36.21602195894604 37.0 37.0 40.0 30.0 40.0 42-43 36.14208784801394 37.0 37.0 40.0 27.0 40.0 44-45 36.05384776142703 37.0 37.0 40.0 27.0 40.0 46-47 35.96936186039552 37.0 37.0 40.0 27.0 40.0 48-49 35.8721780211266 37.0 37.0 40.0 27.0 40.0 50-51 35.7652384071491 37.0 37.0 40.0 27.0 40.0 52-53 35.663973954865604 37.0 37.0 40.0 27.0 40.0 54-55 35.54460995425699 37.0 37.0 40.0 27.0 40.0 56-57 35.42129735041619 37.0 33.0 40.0 27.0 40.0 58-59 35.281652402775876 37.0 33.0 40.0 27.0 40.0 60-61 35.11508537292044 37.0 33.0 40.0 27.0 40.0 62-63 34.923353933333985 37.0 33.0 40.0 27.0 40.0 64-65 34.74043729409442 37.0 33.0 40.0 27.0 40.0 66-67 34.50870689044303 37.0 33.0 40.0 27.0 40.0 68-69 34.28831907663653 37.0 33.0 37.0 27.0 40.0 70-71 34.04802602211362 37.0 33.0 37.0 27.0 40.0 72-73 33.82406710988861 37.0 33.0 37.0 27.0 40.0 74-75 33.58571553438603 37.0 33.0 37.0 27.0 40.0 76-77 33.34337093274084 37.0 33.0 37.0 27.0 37.0 78-79 33.11548859626121 37.0 33.0 37.0 27.0 37.0 80-81 32.89743109682863 37.0 33.0 37.0 27.0 37.0 82-83 32.68579567547464 37.0 33.0 37.0 22.0 37.0 84-85 32.52125986970958 35.0 33.0 37.0 22.0 37.0 86-87 32.56416898403185 33.0 33.0 37.0 22.0 37.0 88-89 32.584820062033806 33.0 33.0 37.0 22.0 37.0 90-91 32.606229027260554 33.0 33.0 37.0 27.0 37.0 92-93 32.58764927986326 33.0 33.0 37.0 22.0 37.0 94-95 32.625817552409146 33.0 33.0 37.0 27.0 37.0 96-97 32.59498517497771 33.0 33.0 37.0 22.0 37.0 98-99 32.529075064647024 33.0 33.0 37.0 22.0 37.0 100 31.99013415718948 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5850825908837081 1101 2 4.072362980885988 1101 3 2.3500860134613752 1101 4 1.1210949252442717 1101 5 0.40992245580632414 1101 6 0.4898486994236748 1101 7 0.5888724738428266 1101 8 0.507035874489759 1101 9 0.6052254349923416 1101 10-11 0.5910337841502837 1101 12-13 0.6602174449689855 1101 14-15 0.8663780784282551 1101 16-17 0.9368388655624003 1101 18-19 1.0119340788277924 1101 20-21 0.968158268713168 1101 22-23 0.9002539089471355 1101 24-25 0.8574949993505783 1101 26-27 0.9435926859064097 1101 28-29 0.8420069403472681 1101 30-31 0.7744470610203606 1101 32-33 1.0022198914381093 1101 34-35 1.1297199528598725 1101 36-37 0.990036532748455 1101 38-39 1.0045170682241036 1101 40-41 0.9692048176028862 1101 42-43 1.0691620291800277 1101 44-45 1.03775829543671 1101 46-47 1.0560257642408288 1101 48-49 0.8682568192516698 1101 50-51 0.886453160792648 1101 52-53 0.9147805469137822 1101 54-55 0.9681043677174301 1101 56-57 0.9098899439120771 1101 58-59 0.9841086701109205 1101 60-61 0.9186907672527482 1101 62-63 0.9097723810227194 1101 64-65 0.9231552461080739 1101 66-67 0.9395728306027635 1101 68-69 0.9499989050765265 1101 70-71 0.9696863269280129 1101 72-73 0.7952061340603791 1101 74-75 0.8087437210478754 1101 76-77 0.846156859240601 1101 78-79 0.8263822983337334 1101 80-81 0.9004715305333377 1101 82-83 0.9329438178311023 1101 84-85 0.9190819783525299 1101 86-87 1.0064734485525477 1101 88-89 0.9629160025593961 1101 90-91 0.9161058811347189 1101 92-93 0.9444780771350878 1101 94-95 0.8317119193976126 1101 96-97 0.7561145174866937 1101 98-99 0.8168274601181622 1101 100 0.8191206255734222 1102 1 0.4091594027678198 1102 2 3.943009555817067 1102 3 2.207225921115569 1102 4 0.9202590408909685 1102 5 0.3860494855448806 1102 6 0.33300542658289345 1102 7 0.4050749517218435 1102 8 0.307148157078835 1102 9 0.43385261000148034 1102 10-11 0.44688245488590184 1102 12-13 0.49985489587909626 1102 14-15 0.7341689037040027 1102 16-17 0.7073204876768528 1102 18-19 0.7898538118178919 1102 20-21 0.7006380452484535 1102 22-23 0.7428624389271192 1102 24-25 0.7444330786009488 1102 26-27 0.6952614206433552 1102 28-29 0.6323985769784954 1102 30-31 0.6550535669826303 1102 32-33 0.7586301679561629 1102 34-35 0.8594395497841489 1102 36-37 0.7068551764406052 1102 38-39 0.7030229281565283 1102 40-41 0.6929192243821163 1102 42-43 0.7774216259088931 1102 44-45 0.8013443730127463 1102 46-47 0.7649031209024741 1102 48-49 0.7111064341175037 1102 50-51 0.6506633490684663 1102 52-53 0.7424514172568308 1102 54-55 0.6345014567520977 1102 56-57 0.6357566693383347 1102 58-59 0.6101680783222818 1102 60-61 0.6365113794255493 1102 62-63 0.70584709278274 1102 64-65 0.6391890301315968 1102 66-67 0.6474382812475383 1102 68-69 0.5678128193756251 1102 70-71 0.5993186200897043 1102 72-73 0.5750893467977036 1102 74-75 0.5467465308123209 1102 76-77 0.6138737507895584 1102 78-79 0.6101348508535551 1102 80-81 0.6198084076118064 1102 82-83 0.6049771225902703 1102 84-85 0.591459330128977 1102 86-87 0.6922616147338587 1102 88-89 0.5615046667231312 1102 90-91 0.6467366845658873 1102 92-93 0.6934694236365289 1102 94-95 0.6401138941518951 1102 96-97 0.644372180702689 1102 98-99 0.6289088815792283 1102 100 0.6923721205748272 1103 1 0.4589671464768834 1103 2 4.000641129061297 1103 3 2.2386943520087073 1103 4 0.9666704647564899 1103 5 0.3504548504640681 1103 6 0.4143069507763215 1103 7 0.3978771126103169 1103 8 0.4057995719603582 1103 9 0.43686540235089666 1103 10-11 0.4717506008916672 1103 12-13 0.5364658788193495 1103 14-15 0.7676313492040236 1103 16-17 0.7444442416229506 1103 18-19 0.8010556385645984 1103 20-21 0.8289787568889722 1103 22-23 0.761927958426277 1103 24-25 0.7403540929523587 1103 26-27 0.6980794283099669 1103 28-29 0.7431066777490329 1103 30-31 0.6827437923510047 1103 32-33 0.6669213231160143 1103 34-35 0.8875175056876756 1103 36-37 0.6905678013907774 1103 38-39 0.682636208978117 1103 40-41 0.6600462553867672 1103 42-43 0.7675096730512578 1103 44-45 0.7833977161706898 1103 46-47 0.7428517347855035 1103 48-49 0.6685331091114435 1103 50-51 0.7226038471419614 1103 52-53 0.6938126443346633 1103 54-55 0.5888937331388249 1103 56-57 0.5713779017881748 1103 58-59 0.5648072833696105 1103 60-61 0.5491346877836207 1103 62-63 0.6310116845262641 1103 64-65 0.6292732025955061 1103 66-67 0.6536957556007792 1103 68-69 0.6492322914079978 1103 70-71 0.7091140042312389 1103 72-73 0.7255390393518155 1103 74-75 0.6938898091974579 1103 76-77 0.6919955430975975 1103 78-79 0.7003840583504655 1103 80-81 0.6736621693663594 1103 82-83 0.7269104295232509 1103 84-85 0.6676634849663756 1103 86-87 0.6892316818577413 1103 88-89 0.6381421288218831 1103 90-91 0.6452776382752958 1103 92-93 0.6234300792321221 1103 94-95 0.6616684676844358 1103 96-97 0.7013673872657407 1103 98-99 0.6893591848877421 1103 100 0.7404098586294872 1104 1 0.3608815158922134 1104 2 3.5506552393985054 1104 3 2.126876964696436 1104 4 0.9167012180555361 1104 5 0.32726504056635264 1104 6 0.2825533588043925 1104 7 0.21586903876380603 1104 8 0.1940059832795029 1104 9 0.2645179648444156 1104 10-11 0.3637475070695828 1104 12-13 0.3277565043293933 1104 14-15 0.46587000927675604 1104 16-17 0.4859923774370216 1104 18-19 0.48838684717327396 1104 20-21 0.4593641285563237 1104 22-23 0.4387705902440331 1104 24-25 0.33435057997420614 1104 26-27 0.3658103065250202 1104 28-29 0.37352904717614877 1104 30-31 0.39997460450341293 1104 32-33 0.5275725908889441 1104 34-35 0.5945179213597171 1104 36-37 0.4475912250865477 1104 38-39 0.4427868210826702 1104 40-41 0.42754926438602325 1104 42-43 0.45242841600110495 1104 44-45 0.5725894122707373 1104 46-47 0.4330571909433658 1104 48-49 0.34559569066798446 1104 50-51 0.41802408460835494 1104 52-53 0.24239255212568622 1104 54-55 0.3194034714155194 1104 56-57 0.40844295700877353 1104 58-59 0.4051075186490962 1104 60-61 0.4411870303005969 1104 62-63 0.4005171419973337 1104 64-65 0.3330795900088148 1104 66-67 0.35806312606909074 1104 68-69 0.3077774473010919 1104 70-71 0.2772632479144832 1104 72-73 0.36259366927104253 1104 74-75 0.32089167476360103 1104 76-77 0.31362029817672266 1104 78-79 0.35825577729505653 1104 80-81 0.31122762565285456 1104 82-83 0.376022791491863 1104 84-85 0.41805076213226045 1104 86-87 0.4111184228844067 1104 88-89 0.3514163783006836 1104 90-91 0.38822091525572233 1104 92-93 0.3597468711766112 1104 94-95 0.35921167439457236 1104 96-97 0.36258486414448754 1104 98-99 0.3977480818613799 1104 100 0.32221784173031764 1105 1 0.4602659716900739 1105 2 3.7265782749853216 1105 3 2.16935887185684 1105 4 0.8961832562169292 1105 5 0.3132608218000641 1105 6 0.33731011137027167 1105 7 0.3488990379884953 1105 8 0.4034953200669804 1105 9 0.3705462022395807 1105 10-11 0.3513275981286341 1105 12-13 0.39751682600363125 1105 14-15 0.46737114684650294 1105 16-17 0.4539673696170681 1105 18-19 0.5510089253835346 1105 20-21 0.5038735031469415 1105 22-23 0.5069000299073849 1105 24-25 0.5336343521608242 1105 26-27 0.5018604130635751 1105 28-29 0.5256057335464348 1105 30-31 0.40903933770253786 1105 32-33 0.5182414177350552 1105 34-35 0.6119057617951782 1105 36-37 0.44322389298836384 1105 38-39 0.4938847429793469 1105 40-41 0.5347899292293761 1105 42-43 0.6305818101295912 1105 44-45 0.585959586288169 1105 46-47 0.6209758527015055 1105 48-49 0.5361173419219867 1105 50-51 0.504175596600291 1105 52-53 0.5379637565059312 1105 54-55 0.44682622762268664 1105 56-57 0.4717078757480593 1105 58-59 0.4828373785849962 1105 60-61 0.5082405776200218 1105 62-63 0.4706988056028507 1105 64-65 0.3777062238450313 1105 66-67 0.4310943283640043 1105 68-69 0.3667163393536157 1105 70-71 0.48070702309541247 1105 72-73 0.4352414664494404 1105 74-75 0.3997484372213904 1105 76-77 0.39201601738547964 1105 78-79 0.42784643933124045 1105 80-81 0.4802825204109453 1105 82-83 0.47205225869215894 1105 84-85 0.39756429399891857 1105 86-87 0.4950493613059308 1105 88-89 0.470829627579505 1105 90-91 0.43716683858539085 1105 92-93 0.42209189567914507 1105 94-95 0.40220576865646507 1105 96-97 0.4747824189710741 1105 98-99 0.457038831855364 1105 100 0.39886255904891144 1106 1 0.4480226635825133 1106 2 3.876700166238656 1106 3 2.175371933823225 1106 4 0.9595882957746156 1106 5 0.3457591327526828 1106 6 0.24836736841064067 1106 7 0.2058792478168172 1106 8 0.14740279625041808 1106 9 0.22703471605112213 1106 10-11 0.1900530636327531 1106 12-13 0.2976661950928019 1106 14-15 0.30686981626361387 1106 16-17 0.2796867039125246 1106 18-19 0.35921296497186717 1106 20-21 0.2695951428088392 1106 22-23 0.21455692475203136 1106 24-25 0.2331432291542015 1106 26-27 0.3007991582779397 1106 28-29 0.2432590837236006 1106 30-31 0.2489198450963741 1106 32-33 0.41293529039087673 1106 34-35 0.4220191245252636 1106 36-37 0.23915668022969072 1106 38-39 0.20485039834971985 1106 40-41 0.31340469716691643 1106 42-43 0.2979376481036482 1106 44-45 0.22407206418396441 1106 46-47 0.1823998628930923 1106 48-49 0.10934212314537461 1106 50-51 0.1483028821445771 1106 52-53 0.09636416534201686 1106 54-55 0.06579714136067594 1106 56-57 0.1883259698096751 1106 58-59 0.11375999156460637 1106 60-61 0.12588121496722948 1106 62-63 0.2505868687352475 1106 64-65 0.16891320977163815 1106 66-67 0.034654706977143235 1106 68-69 0.08725431593482114 1106 70-71 0.15028648694446645 1106 72-73 0.10948399366408523 1106 74-75 0.05501050941268204 1106 76-77 0.07169525163069324 1106 78-79 0.07903363641874961 1106 80-81 0.11375051113084567 1106 82-83 0.24721686585371572 1106 84-85 0.2547775190733077 1106 86-87 0.30550249240665295 1106 88-89 0.2480098446037715 1106 90-91 0.24464351507318582 1106 92-93 0.26451420949415194 1106 94-95 0.2614187858481003 1106 96-97 0.23025791745659063 1106 98-99 0.2510450923244605 1106 100 0.17788503037508718 1107 1 0.3503467945753762 1107 2 3.4194235379252156 1107 3 1.9816025300217035 1107 4 0.8201481444614984 1107 5 0.20755452430599775 1107 6 0.08412881215036094 1107 7 0.08468711422327146 1107 8 0.015693787512141455 1107 9 0.008185823508419787 1107 10-11 -0.019604546757804542 1107 12-13 0.02291703735388495 1107 14-15 -0.06884695811704233 1107 16-17 -0.08813511401390883 1107 18-19 -0.10030882750660197 1107 20-21 -0.0011990091065925412 1107 22-23 -0.087716725175909 1107 24-25 -0.10468628953677239 1107 26-27 -0.12212831846319006 1107 28-29 -0.10854867958489933 1107 30-31 -0.10798006616229827 1107 32-33 -0.006541603086660075 1107 34-35 0.07253421017632888 1107 36-37 -0.0434207093492347 1107 38-39 -0.17473874557428815 1107 40-41 -0.06540453424367598 1107 42-43 0.011660227445439375 1107 44-45 0.08576206938811026 1107 46-47 -0.07623270432854667 1107 48-49 -0.13581125567881003 1107 50-51 -0.0919272746831652 1107 52-53 -0.044602582514741584 1107 54-55 -0.1821614949455821 1107 56-57 -0.20435498236582816 1107 58-59 -0.09527039942289406 1107 60-61 -0.17526672139809563 1107 62-63 -0.022116268236977987 1107 64-65 -0.13426907791320986 1107 66-67 -0.11471283842744384 1107 68-69 -0.16150465441739925 1107 70-71 -0.1391035847755262 1107 72-73 -0.013758469826868236 1107 74-75 -0.052401679870897055 1107 76-77 -0.10379571627072437 1107 78-79 -0.1803020952893135 1107 80-81 -0.04394469704084969 1107 82-83 0.02095740597354734 1107 84-85 0.08280838229871534 1107 86-87 -0.15861358283494553 1107 88-89 -0.07207787216825068 1107 90-91 -0.06587307368832285 1107 92-93 -0.02076730681882566 1107 94-95 -0.10759833408737052 1107 96-97 -0.14763138145693944 1107 98-99 -0.11502278895679297 1107 100 -0.012935723597529147 1108 1 0.4171685439944852 1108 2 3.6558545644695286 1108 3 2.121912517065425 1108 4 0.9251116848454757 1108 5 0.23735118565452495 1108 6 0.04680498008945477 1108 7 0.026101490340884936 1108 8 0.05089236125051144 1108 9 0.0074141217384422475 1108 10-11 0.01042329318394053 1108 12-13 0.0059881041590372774 1108 14-15 0.024773519230883778 1108 16-17 -0.010526832702005606 1108 18-19 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0.30358826923995963 2210 60-61 0.32643997014765347 2210 62-63 0.2728624120703742 2210 64-65 0.03362839430268849 2210 66-67 0.2598868057550803 2210 68-69 0.22082865322956025 2210 70-71 0.3007114763702816 2210 72-73 0.37638059132383717 2210 74-75 -0.10997157739448227 2210 76-77 0.1961765179792465 2210 78-79 0.26990876234211214 2210 80-81 0.3130295058864192 2210 82-83 0.3724667558146564 2210 84-85 0.4450429411168457 2210 86-87 0.45125928183210107 2210 88-89 0.587090589486877 2210 90-91 0.4769190538041741 2210 92-93 0.5834826876769483 2210 94-95 0.566840143370996 2210 96-97 0.3746811355144217 2210 98-99 0.3929769884279395 2210 100 0.34796459613670194 2211 1 0.37720602703018 2211 2 3.8007053862958564 2211 3 2.1125053522046926 2211 4 0.8188203511818344 2211 5 0.3404125156560269 2211 6 0.15843678466065825 2211 7 0.011303661771918883 2211 8 0.0734084638764756 2211 9 0.047745437306829785 2211 10-11 0.057802604271600444 2211 12-13 -0.03601853076911965 2211 14-15 -0.07273536683361215 2211 16-17 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-0.11367092656514899 2211 80-81 -0.1404214100186323 2211 82-83 -0.4928414947131827 2211 84-85 -0.19718352391290495 2211 86-87 -0.14028102340396487 2211 88-89 -0.01232678834733747 2211 90-91 -0.08269075144911398 2211 92-93 -0.08028431021169524 2211 94-95 -0.08010558888549468 2211 96-97 -0.1279483695720316 2211 98-99 -0.06724180017565828 2211 100 -0.29179286866173726 2212 1 0.512716906390505 2212 2 4.026378388910029 2212 3 2.2719755152207277 2212 4 1.1453650696911097 2212 5 0.6188436502398105 2212 6 0.5353055543820275 2212 7 0.5572062204233745 2212 8 0.47623676276803195 2212 9 0.2858022739085726 2212 10-11 0.26604770583926296 2212 12-13 0.28375549826296975 2212 14-15 0.5130715248693889 2212 16-17 0.45540230307467766 2212 18-19 0.43131937020772426 2212 20-21 0.4172472279669961 2212 22-23 0.45981909158207657 2212 24-25 0.34115208816621134 2212 26-27 0.34334687155873667 2212 28-29 0.33560702814536825 2212 30-31 0.31036112321339004 2212 32-33 0.5064616031216218 2212 34-35 0.4783691764293181 2212 36-37 0.6079623840038479 2212 38-39 0.4691635995895638 2212 40-41 0.5321818302184482 2212 42-43 0.4396124144483693 2212 44-45 0.4051020439547841 2212 46-47 0.4351519699403852 2212 48-49 0.5304040179097669 2212 50-51 0.45136295095790757 2212 52-53 0.3989533479305081 2212 54-55 0.37465596197099416 2212 56-57 0.45608336122266024 2212 58-59 0.4085794426935294 2212 60-61 0.3151928328113698 2212 62-63 0.5475827059705196 2212 64-65 0.5478522546046989 2212 66-67 0.4773441341741673 2212 68-69 0.41105712957680396 2212 70-71 0.47223050712005943 2212 72-73 0.43485049757160255 2212 74-75 0.3925302714091572 2212 76-77 0.5305386210828473 2212 78-79 0.3743445492168007 2212 80-81 0.36592541824119706 2212 82-83 0.5824595395165559 2212 84-85 0.6927595195180558 2212 86-87 0.5347774955299442 2212 88-89 0.6415028190247938 2212 90-91 0.5587032685993734 2212 92-93 0.6492971155020371 2212 94-95 0.620072269614468 2212 96-97 0.5502034225441648 2212 98-99 0.7077596645413919 2212 100 0.48298093717537327 2213 1 -0.7362012192113419 2213 2 -25.96512904208785 2213 3 -14.828743220639494 2213 4 -6.098294508232851 2213 5 -2.0975486321961156 2213 6 -1.368149482569926 2213 7 -1.244062444085344 2213 8 -1.0601641050518609 2213 9 -1.101456552214195 2213 10-11 -1.1459883643752562 2213 12-13 -1.2278188175167344 2213 14-15 -1.4528494315887883 2213 16-17 -1.5466106214087532 2213 18-19 -1.341958332157894 2213 20-21 -1.4444120715120832 2213 22-23 -1.5252057944295458 2213 24-25 -1.4462059339469562 2213 26-27 -1.6424727425630579 2213 28-29 -1.6060964744592567 2213 30-31 -1.610627240110773 2213 32-33 -1.5650639267937976 2213 34-35 -1.441029730674508 2213 36-37 -1.5477115106279697 2213 38-39 -1.5968582931395332 2213 40-41 -1.5136267041172857 2213 42-43 -1.482795727744147 2213 44-45 -1.3533484682677894 2213 46-47 -1.3854584624613153 2213 48-49 -1.5800370851960537 2213 50-51 -1.6199551955273037 2213 52-53 -1.5769089610737055 2213 54-55 -1.3452589383109341 2213 56-57 -1.3067207528005156 2213 58-59 -1.542853678325578 2213 60-61 -1.4872592224599472 2213 62-63 -1.3021411695182152 2213 64-65 -1.3683793419286587 2213 66-67 -1.5352482457174617 2213 68-69 -1.5709237157354607 2213 70-71 -1.4834677514392212 2213 72-73 -1.4792071985290107 2213 74-75 -1.8229251600063812 2213 76-77 -1.607214734758113 2213 78-79 -1.4281089562882912 2213 80-81 -1.5270212247576538 2213 82-83 -1.6310598298057783 2213 84-85 -1.5715020794517045 2213 86-87 -1.3559386879188224 2213 88-89 -1.4370780982190645 2213 90-91 -1.4299225478822422 2213 92-93 -1.3204437567265614 2213 94-95 -1.4181747458044214 2213 96-97 -1.6690980288498025 2213 98-99 -1.5060657041080283 2213 100 -1.6053673045611987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 21.0 5 46.0 6 40.0 7 39.0 8 43.0 9 84.0 10 335.0 11 1416.0 12 4045.0 13 7719.0 14 10103.0 15 11456.0 16 12281.0 17 12965.0 18 13683.0 19 14561.0 20 16042.0 21 18242.0 22 19915.0 23 21493.0 24 22920.0 25 25079.0 26 28085.0 27 32403.0 28 38684.0 29 46529.0 30 57346.0 31 71019.0 32 91308.0 33 123082.0 34 170168.0 35 245439.0 36 379540.0 37 655292.0 38 625893.0 39 2767.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.80631988772693 20.50674996604645 10.686875820544161 30.00005432568247 2 13.411643470285414 22.739391640841145 35.84558629099156 28.003378597881877 3 15.480503018419862 26.83812830991149 28.815505358266275 28.86586331340237 4 11.051345482067374 18.888081115624008 37.475003893978744 32.585569508329876 5 12.18172706714611 38.29459928098012 34.95799112519513 14.565682526678636 6 29.801403395371757 39.53753526573421 17.856239250226828 12.804822088667207 7 26.706019934511172 32.883019682631065 22.793422330970458 17.6175380518873 8 24.950880268765896 36.10778848216072 20.754327934121264 18.18700331495212 9 24.86563415353835 17.07273891859603 21.428977538521327 36.63264938934429 10-11 23.376680158009748 27.387684343954582 28.995166326759193 20.240469171276484 12-13 24.311778459279758 24.711635601751354 29.128038308847543 21.84854763012135 14-15 21.767720704195067 26.442562616558423 26.129167202696735 25.66054947654977 16-17 21.35126372989766 29.97707215936936 26.73049064571449 21.94117346501849 18-19 21.238207786894904 28.981333047412438 28.687664462233347 21.092794703459315 20-21 22.53815284740516 28.168544597222933 27.922922937328522 21.37037961804339 22-23 21.88032589005438 28.044281903172013 28.20161433945022 21.873777867323387 24-25 21.949659773635734 27.96002873130563 28.069668919012507 22.02064257604613 26-27 21.757956847824836 28.06400517457723 28.02175308229823 22.15628489529971 28-29 21.65839173097648 27.991995908732203 28.2899727422701 22.059639618021222 30-31 22.233221703489644 28.24981003258229 27.73518901758062 21.781779246347444 32-33 21.50692810532749 28.1891388401588 28.349207730931745 21.95472532358196 34-35 21.745412099449553 27.74091030493678 28.332006895677253 22.181670699936422 36-37 22.090196986886966 28.219840991776206 27.74025155558561 21.949710465751217 38-39 21.875148385351544 28.21819949034578 28.009848470677618 21.896803653625064 40-41 22.052064957451893 27.90091916578654 27.917304576547746 22.12971130021382 42-43 21.720223749055723 28.02399366883701 28.063113781071262 22.192668801036007 44-45 21.989225379928385 27.727757381111175 28.065759284800055 22.21725795416038 46-47 22.044068169782648 28.064614656244242 27.83521510637318 22.056102067599927 48-49 21.876829299445095 27.94070398368575 27.96569129214787 22.21677542472128 50-51 22.15807811857181 27.810828221063062 27.66497493888751 22.366118721477616 52-53 21.864744104414473 27.89889637725991 27.814056289850868 22.422303228474753 54-55 22.145488443581122 27.948361025252826 27.629107330908155 22.277043200257893 56-57 22.113016279140464 27.83290339489179 27.65484214534755 22.39923818062019 58-59 21.776640880213417 27.480335483513535 28.094450837044928 22.648572799228113 60-61 22.24179025495182 27.87288689079509 27.502009615576593 22.383313238676497 62-63 21.803734407440466 28.057451204503074 27.771314043583793 22.367500344472663 64-65 21.87965281636543 27.520622370430786 27.867798809634525 22.73192600356926 66-67 21.87695513536597 27.756617194434043 27.698621965728847 22.667805704471135 68-69 22.08927906124526 27.737235600591003 27.735382643694557 22.438102694469176 70-71 22.186894135251727 27.58181923177182 27.582161039108694 22.649125593867762 72-73 21.88761153739464 27.877431821918663 27.794303835040683 22.44065280564601 74-75 22.085256850996952 27.519443853792147 27.642545386381943 22.75275390882895 76-77 22.064144063764513 27.81216019196989 27.49605384223104 22.627641902034554 78-79 22.007992931626582 27.729425342104523 27.73386825801002 22.528713468258875 80-81 22.18479667983516 27.47672760218891 27.56268883275657 22.77578688521936 82-83 21.770465136189625 27.70765869244433 27.827106552311363 22.69476961905468 84-85 21.89832343751967 27.338238970859365 27.714507724490474 23.04892986713049 86-87 21.875243380718942 27.70046252454042 27.584629040569595 22.83966505417104 88-89 21.71826397634729 27.404058357641826 27.845963855443358 23.031713810567528 90-91 21.86074302593682 27.374990984353875 27.65993036773584 23.104335621973462 92-93 21.603527062412624 27.727648709590913 27.70890763835344 22.959916589643022 94-95 21.558108575237323 27.207854642143005 27.925068604560398 23.308968178059274 96-97 21.61989310733492 27.43075420352339 27.7938734463793 23.15547924276239 98-99 21.49810554319482 27.31800058595341 27.83704955730662 23.346844313545144 100 21.706568606057203 27.142969286201442 27.627258775293857 23.523203332447505 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6.0 1 5.5 2 6.0 3 12.0 4 14.0 5 14.5 6 28.0 7 45.5 8 70.5 9 93.0 10 100.5 11 100.5 12 121.0 13 153.5 14 183.0 15 207.5 16 245.0 17 302.5 18 354.0 19 471.0 20 615.0 21 808.0 22 1154.0 23 1644.0 24 2280.0 25 3324.5 26 4985.5 27 7539.0 28 11375.0 29 17112.0 30 24872.0 31 34594.0 32 46271.0 33 59478.0 34 72383.5 35 84135.0 36 95163.5 37 105099.5 38 113118.0 39 118955.0 40 122463.5 41 123672.5 42 123637.0 43 121634.5 44 117738.5 45 114204.0 46 110129.0 47 104855.0 48 100196.5 49 96363.5 50 94709.0 51 89795.5 52 80642.0 53 73833.0 54 68370.5 55 63341.0 56 58107.0 57 52335.0 58 46947.0 59 41709.5 60 35634.5 61 29341.0 62 24016.0 63 19068.0 64 14930.5 65 11784.5 66 9088.5 67 6792.5 68 4965.0 69 3604.0 70 2594.5 71 1898.0 72 1414.0 73 1029.5 74 700.5 75 445.5 76 279.5 77 177.5 78 104.5 79 43.5 80 22.0 81 15.5 82 8.5 83 5.5 84 4.0 85 2.0 86 1.0 87 1.5 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6824222320300335 2 4.778430392896363 3 0.0 4 0.00589907474451431 5 0.15733263983235116 6 0.0557534503292511 7 0.03586205805049248 8 0.04219277241045906 9 0.040178454205015146 10-11 0.041527328003303485 12-13 0.03972882960558571 14-15 0.04242657720216237 16-17 0.033919679780957286 18-19 0.02782277021269402 20-21 0.022265410163746086 22-23 0.022733019747152708 24-25 0.01980146735887273 26-27 0.01269739868788751 28-29 0.018650428384333353 30-31 0.024207788433281283 32-33 0.0015826785899916442 34-35 0.003399161971686599 36-37 0.004136546314750888 38-39 0.006222804456103511 40-41 0.006654444071555776 42-43 0.007086083687008043 44-45 0.008309062597456132 46-47 0.016150515611505642 48-49 0.02467539801668791 50-51 0.021276236045001305 52-53 0.026995460949743838 54-55 0.023236599298513686 56-57 0.015664921044121843 58-59 0.014657761941399885 60-61 0.012103894216640642 62-63 0.017481404425816797 64-65 0.01908206799978562 66-67 0.020071242118530397 68-69 0.02717531078951562 70-71 0.027103370853606906 72-73 0.023308539234422394 74-75 0.024603458080779194 76-77 0.01778714915342882 78-79 0.014064257470153019 80-81 0.012787323607773397 82-83 0.008021302853821287 84-85 0.010862930322215376 86-87 0.00879465716483993 88-89 0.007337873462688532 90-91 0.007463768350528777 92-93 0.003830801587138866 94-95 0.0012229789104480887 96-97 6.654444071555777E-4 98-99 4.4962459942944437E-4 100 0.10931273261328653 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2780097.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.37114231681377 #Duplication Level Percentage of deduplicated Percentage of total 1 89.25987217205585 75.30957378209129 2 7.980771969434574 13.466936952624053 3 1.584482269247581 4.010537371114672 4 0.5010190863903785 1.690862105651306 5 0.229736632182435 0.9691571044624863 6 0.11721740239820036 0.5933859683847474 7 0.0772748963877853 0.45638398964556115 8 0.0500148203648169 0.33758460215598746 9 0.037784988657498714 0.2869166389914916 >10 0.15511225818306665 2.1526679103295554 >50 0.004777763462393836 0.27253769305493325 >100 0.0016368277094567106 0.2305860051628479 >500 2.1350966142124502E-4 0.1115069944295603 >1k 8.540386448363082E-5 0.11136288190147761 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.596996795435555E-5 0.0 4 0.0 0.0 0.0 3.596996795435555E-5 0.0 5 0.0 0.0 0.0 3.596996795435555E-5 0.0 6 0.0 0.0 0.0 3.596996795435555E-5 0.0 7 0.0 3.596996795435555E-5 0.0 7.19399359087111E-5 0.0 8 0.0 3.596996795435555E-5 0.0 7.19399359087111E-5 0.0 9 0.0 3.596996795435555E-5 0.0 1.7984983977177774E-4 0.0 10-11 0.0 3.596996795435555E-5 0.0 1.7984983977177774E-4 3.596996795435555E-5 12-13 0.0 3.596996795435555E-5 0.0 1.7984983977177774E-4 3.596996795435555E-5 14-15 0.0 3.596996795435555E-5 0.0 1.9783482374895553E-4 3.596996795435555E-5 16-17 0.0 3.596996795435555E-5 0.0 2.158198077261333E-4 3.596996795435555E-5 18-19 0.0 3.596996795435555E-5 0.0 2.158198077261333E-4 3.596996795435555E-5 20-21 0.0 3.596996795435555E-5 0.0 2.338047917033111E-4 3.596996795435555E-5 22-23 0.0 3.596996795435555E-5 0.0 2.5178977568048885E-4 3.596996795435555E-5 24-25 0.0 3.596996795435555E-5 0.0 2.877597436348444E-4 3.596996795435555E-5 26-27 1.7984983977177776E-5 3.596996795435555E-5 0.0 5.395495193153332E-4 3.596996795435555E-5 28-29 3.596996795435555E-5 3.596996795435555E-5 0.0 0.0011690239585165553 3.596996795435555E-5 30-31 3.596996795435555E-5 3.596996795435555E-5 0.0 0.002949537372257155 3.596996795435555E-5 32-33 3.596996795435555E-5 3.596996795435555E-5 0.0 0.008668762276999687 3.596996795435555E-5 34-35 3.596996795435555E-5 3.596996795435555E-5 0.0 0.01663611017888944 3.596996795435555E-5 36-37 3.596996795435555E-5 3.596996795435555E-5 0.0 0.03012484816177277 3.596996795435555E-5 38-39 3.596996795435555E-5 3.596996795435555E-5 0.0 0.05201257366199813 3.596996795435555E-5 40-41 3.596996795435555E-5 3.596996795435555E-5 0.0 0.07875624483606147 3.596996795435555E-5 42-43 7.19399359087111E-5 3.596996795435555E-5 0.0 0.10832355849454174 5.395495193153333E-5 44-45 7.19399359087111E-5 3.596996795435555E-5 0.0 0.14497695584003006 7.19399359087111E-5 46-47 7.19399359087111E-5 3.596996795435555E-5 0.0 0.18754741291400984 7.19399359087111E-5 48-49 7.19399359087111E-5 3.596996795435555E-5 0.0 0.2321501731774107 7.19399359087111E-5 50-51 7.19399359087111E-5 3.596996795435555E-5 0.0 0.28369513725600226 7.19399359087111E-5 52-53 7.19399359087111E-5 3.596996795435555E-5 0.0 0.3389270230499152 7.19399359087111E-5 54-55 7.19399359087111E-5 3.596996795435555E-5 0.0 0.4038708001915041 7.19399359087111E-5 56-57 7.19399359087111E-5 3.596996795435555E-5 0.0 0.47672797028305125 7.19399359087111E-5 58-59 7.19399359087111E-5 3.596996795435555E-5 0.0 0.5589193470587537 7.19399359087111E-5 60-61 7.19399359087111E-5 3.596996795435555E-5 0.0 0.6520096241246258 7.19399359087111E-5 62-63 7.19399359087111E-5 3.596996795435555E-5 0.0 0.7493803273770663 7.19399359087111E-5 64-65 7.19399359087111E-5 7.19399359087111E-5 0.0 0.8535133846049257 7.19399359087111E-5 66-67 1.0790990386306665E-4 7.19399359087111E-5 0.0 0.98010968682028 7.19399359087111E-5 68-69 1.0790990386306665E-4 7.19399359087111E-5 0.0 1.1211839011372624 7.19399359087111E-5 70-71 1.0790990386306665E-4 7.19399359087111E-5 0.0 1.2764842377801926 7.19399359087111E-5 72-73 1.0790990386306665E-4 7.19399359087111E-5 0.0 1.4385469283985415 7.19399359087111E-5 74-75 1.0790990386306665E-4 7.19399359087111E-5 0.0 1.612623588313645 7.19399359087111E-5 76-77 1.0790990386306665E-4 7.19399359087111E-5 0.0 1.8036241181512733 7.19399359087111E-5 78-79 1.0790990386306665E-4 7.19399359087111E-5 0.0 2.0264220996605515 7.19399359087111E-5 80-81 1.2589488784024442E-4 7.19399359087111E-5 0.0 2.2740753290262896 7.19399359087111E-5 82-83 1.438798718174222E-4 8.992491988588889E-5 0.0 2.542015620318284 7.19399359087111E-5 84-85 1.438798718174222E-4 1.0790990386306665E-4 0.0 2.820962721804311 7.19399359087111E-5 86-87 1.438798718174222E-4 1.0790990386306665E-4 0.0 3.1242974615633914 7.19399359087111E-5 88 1.438798718174222E-4 1.0790990386306665E-4 0.0 3.371608976233563 7.19399359087111E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5130 0.0 24.565128 1 TCAACGC 5545 0.0 21.967796 4 ATCAACG 5610 0.0 21.796316 3 CAACGCA 5630 0.0 21.636131 5 AACGCAG 5840 0.0 20.909262 6 TATCAAC 6040 0.0 20.277912 2 GTACATG 7895 0.0 18.40312 1 ACGCAGA 6670 0.0 18.16294 7 CGCAGAG 6815 0.0 17.707592 8 TACATGG 8045 0.0 17.618315 2 GTATTAG 1235 0.0 17.606861 1 GGTATCA 3675 0.0 17.34717 1 TATACCG 220 3.1788855E-5 17.10234 5 ACATGGG 8220 0.0 16.70643 3 TACACCG 205 3.5956566E-4 16.059515 5 ATACCGT 295 1.3861918E-6 15.920481 6 TCTATAC 1225 0.0 14.972734 3 GATACCG 190 0.0040116506 14.852034 5 GCAGAGT 8040 0.0 14.658923 9 CATGGGG 4575 0.0 14.494936 4 >>END_MODULE