FastQCFastQC Report
Wed 25 May 2016
SRR1295107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895759
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34640.11962321450093048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA12300.019.0095391
GTACATG134700.015.6225391
TACATGG135900.015.3924932
TATACTG13900.015.1575125
GTATTAG12700.015.0193481
CCTATAC7850.014.9135983
GTATTAT16450.014.5878661
CATGGGG37800.014.3080974
TGTACCG2509.93599E-414.045965
ACATGGG136450.013.9728633
CAGCGTA3605.4613392E-613.9516089
GTGCTAG8851.8189894E-1213.2100191
GTATAAG11250.013.1265861
GTATTAC9155.456968E-1212.7769021
TTATACT17000.012.4634024
GTCTCGC3954.6956027E-412.4619491
TCTAGAC9850.012.451433
GTGTACA13450.012.3519231
GTATATA18500.012.3061751
GTCTAGT8001.1696102E-912.3061741
Produced by FastQC (version 0.11.5)