##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295107_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2895759 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.316896882648038 33.0 27.0 33.0 15.0 33.0 2 22.329612719843052 27.0 6.0 33.0 2.0 33.0 3 24.73537196983589 27.0 15.0 33.0 15.0 33.0 4 25.082194340067666 33.0 15.0 37.0 2.0 37.0 5 26.847968356482706 33.0 15.0 37.0 2.0 37.0 6 27.5719115437438 33.0 15.0 37.0 6.0 37.0 7 27.17977842769374 33.0 15.0 37.0 6.0 37.0 8 28.08311085280232 33.0 22.0 37.0 6.0 37.0 9 29.00852937002009 33.0 27.0 37.0 15.0 37.0 10-11 28.53953436732822 33.0 24.5 37.0 10.5 37.0 12-13 27.343034244217147 33.0 15.0 37.0 4.0 37.0 14-15 29.04732317157609 33.0 22.0 37.0 10.5 40.0 16-17 29.476859262114008 33.0 27.0 37.0 6.0 40.0 18-19 29.628300904875026 33.0 27.0 37.0 10.5 40.0 20-21 29.147647646092096 33.0 22.0 37.0 6.0 40.0 22-23 29.885011321729465 33.0 27.0 37.0 15.0 40.0 24-25 30.00630905403385 33.0 27.0 37.0 15.0 40.0 26-27 28.60047573019716 33.0 18.5 37.0 4.0 40.0 28-29 29.29441607537091 33.0 22.0 37.0 6.0 40.0 30-31 30.02180620003253 33.0 27.0 37.0 15.0 40.0 32-33 28.825897631674458 33.0 22.0 37.0 6.0 40.0 34-35 28.38406062106688 33.0 22.0 37.0 6.0 40.0 36-37 28.662509034764287 33.0 22.0 37.0 6.0 40.0 38-39 28.766351412531222 33.0 22.0 37.0 6.0 40.0 40-41 27.940243127967484 33.0 22.0 37.0 6.0 40.0 42-43 28.482233673451418 33.0 22.0 37.0 6.0 40.0 44-45 28.272714856450417 33.0 22.0 37.0 6.0 40.0 46-47 27.996447736154842 33.0 22.0 37.0 6.0 40.0 48-49 27.821185740940457 33.0 22.0 37.0 6.0 40.0 50-51 27.7512246357518 33.0 22.0 37.0 6.0 40.0 52-53 28.005666735387855 33.0 22.0 37.0 6.0 40.0 54-55 27.980682266721782 33.0 22.0 37.0 6.0 40.0 56-57 27.95884015209829 33.0 22.0 37.0 6.0 40.0 58-59 27.739284588254755 33.0 22.0 37.0 6.0 40.0 60-61 27.732134994659432 33.0 22.0 37.0 6.0 40.0 62-63 27.638354918347833 33.0 22.0 37.0 6.0 40.0 64-65 27.233158733168057 33.0 22.0 37.0 6.0 38.5 66-67 27.46334208060823 33.0 22.0 37.0 6.0 37.0 68-69 27.030675204670004 33.0 22.0 37.0 6.0 37.0 70-71 27.128045358747052 33.0 22.0 37.0 6.0 37.0 72-73 27.12836151074727 27.0 22.0 37.0 10.5 37.0 74-75 27.2274018659702 27.0 22.0 37.0 10.5 37.0 76-77 26.98162502473445 27.0 22.0 35.0 6.0 37.0 78-79 26.56948540952476 27.0 22.0 33.0 6.0 37.0 80-81 26.400529187684473 27.0 22.0 33.0 6.0 37.0 82-83 26.478475246040848 27.0 22.0 33.0 10.5 37.0 84-85 26.42288222189761 27.0 22.0 33.0 10.5 37.0 86-87 26.21865821706848 27.0 22.0 33.0 10.5 37.0 88-89 26.14517558263654 27.0 22.0 33.0 6.0 37.0 90-91 26.277435725832156 27.0 22.0 33.0 10.5 37.0 92-93 26.197374850600482 27.0 22.0 33.0 10.5 37.0 94-95 26.079257458925277 27.0 22.0 33.0 10.5 37.0 96-97 26.163672805644392 27.0 22.0 33.0 10.5 37.0 98-99 26.23001240780051 27.0 22.0 33.0 10.5 37.0 100 25.876232794234603 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.435303850226994 1101 2 11.285426216917802 1101 3 8.539383468770634 1101 4 12.817458754344084 1101 5 10.8108416406027 1101 6 10.01900369194161 1101 7 9.570050502234047 1101 8 9.166044307902904 1101 9 7.420130901541551 1101 10-11 7.720952152344061 1101 12-13 9.25999656750821 1101 14-15 8.12066659149065 1101 16-17 8.298309937788762 1101 18-19 8.255850512442397 1101 20-21 7.20614235841326 1101 22-23 7.403049367649441 1101 24-25 7.522969384194614 1101 26-27 8.609597319028843 1101 28-29 8.244096816335233 1101 30-31 5.467487110064763 1101 32-33 6.886533985803926 1101 34-35 8.791636005400548 1101 36-37 8.272666913047491 1101 38-39 8.323472694014846 1101 40-41 9.11681152754603 1101 42-43 8.763185982244579 1101 44-45 8.60156279369259 1101 46-47 7.471981090610509 1101 48-49 5.496151856594686 1101 50-51 6.820697424226484 1101 52-53 5.913217532409398 1101 54-55 4.090934120957705 1101 56-57 6.257518899399976 1101 58-59 6.339443731829736 1101 60-61 6.805924083586415 1101 62-63 6.404166484110679 1101 64-65 6.804774795455767 1101 66-67 5.869939522238948 1101 68-69 6.893920596343978 1101 70-71 6.492458734459756 1101 72-73 5.696077493787929 1101 74-75 5.060501085078183 1101 76-77 5.135881560013395 1101 78-79 5.273303552406112 1101 80-81 5.094337337171595 1101 82-83 4.568067290529072 1101 84-85 4.333486921859844 1101 86-87 4.108236053417052 1101 88-89 4.1390292255979055 1101 90-91 3.9780683557947683 1101 92-93 3.963004487216388 1101 94-95 3.8276538761701318 1101 96-97 3.9446090091764567 1101 98-99 3.70969013502636 1101 100 3.9133315406283415 1102 1 6.678303178478622 1102 2 10.435015829467641 1102 3 8.1401532510075 1102 4 12.44471837850369 1102 5 10.48245594413888 1102 6 9.35442581146766 1102 7 6.5121818262181 1102 8 7.785807446040334 1102 9 6.421663974329345 1102 10-11 7.330155010943848 1102 12-13 9.091329395305422 1102 14-15 6.076619796784097 1102 16-17 7.276433191384118 1102 18-19 7.402815696307631 1102 20-21 6.817262851630858 1102 22-23 6.418069858797228 1102 24-25 6.968045487451928 1102 26-27 8.488392603242382 1102 28-29 7.542428123760782 1102 30-31 4.003275586475482 1102 32-33 4.169047218494175 1102 34-35 4.894079748826542 1102 36-37 4.920343595099769 1102 38-39 4.425579622431307 1102 40-41 6.119476411664728 1102 42-43 6.91224097152859 1102 44-45 6.406714605061133 1102 46-47 5.539142050552098 1102 48-49 3.672095361992195 1102 50-51 4.9740122295982125 1102 52-53 5.941371927280773 1102 54-55 5.223546798749929 1102 56-57 6.335136648233821 1102 58-59 6.0711014261710545 1102 60-61 6.125002913351832 1102 62-63 4.344718870134553 1102 64-65 6.180816993296013 1102 66-67 3.7219380206837656 1102 68-69 5.99946106212715 1102 70-71 5.356205067598026 1102 72-73 4.952871654657553 1102 74-75 4.246158363575272 1102 76-77 3.2542727414814223 1102 78-79 4.334195566503425 1102 80-81 4.416406340970642 1102 82-83 3.7629926267325438 1102 84-85 3.0190437673897073 1102 86-87 3.280012413895669 1102 88-89 3.408160412221651 1102 90-91 3.3431574800569273 1102 92-93 3.228973329005534 1102 94-95 3.1632535762354266 1102 96-97 3.3699830808183293 1102 98-99 3.0309544990575255 1102 100 3.110336333186048 1103 1 6.703313371057941 1103 2 10.894734393417817 1103 3 7.9512977487470025 1103 4 11.965283015168456 1103 5 10.278992018215167 1103 6 9.032790510818803 1103 7 7.945574126107147 1103 8 8.055895589693659 1103 9 7.203077060634303 1103 10-11 7.453479278387455 1103 12-13 8.768241702462227 1103 14-15 8.572410976617451 1103 16-17 8.020735469042865 1103 18-19 7.2970830956045525 1103 20-21 8.307463069341132 1103 22-23 6.864658527388531 1103 24-25 5.1615382104337435 1103 26-27 6.000127061976137 1103 28-29 6.9852047979020035 1103 30-31 6.08996528649136 1103 32-33 7.875166704127469 1103 34-35 7.075860775158851 1103 36-37 4.071603184383136 1103 38-39 6.433089800910221 1103 40-41 6.9824257513225625 1103 42-43 7.558582490214853 1103 44-45 5.701878860692634 1103 46-47 6.497436171327319 1103 48-49 5.636222619366503 1103 50-51 6.374482196135933 1103 52-53 6.1114553070111555 1103 54-55 5.0207228298320565 1103 56-57 6.056082005936958 1103 58-59 5.650993672881434 1103 60-61 5.163561632277556 1103 62-63 2.8869566871821846 1103 64-65 5.04093633107648 1103 66-67 4.192565414227715 1103 68-69 5.170876174861913 1103 70-71 3.735264977403773 1103 72-73 4.460715458818221 1103 74-75 3.2865777821712108 1103 76-77 3.6670974335486974 1103 78-79 2.9973772002812282 1103 80-81 4.118545533734476 1103 82-83 3.582520118093022 1103 84-85 3.4174653981379386 1103 86-87 2.8449861790828095 1103 88-89 3.015682253750807 1103 90-91 2.7691957131068285 1103 92-93 2.3015975213544095 1103 94-95 2.7502171262825996 1103 96-97 3.0099558992639146 1103 98-99 2.878087463470518 1103 100 3.0475647492914497 1104 1 6.40815864492404 1104 2 9.659556306244 1104 3 7.425992038277435 1104 4 12.147934863984997 1104 5 9.329035149690927 1104 6 8.038036022358 1104 7 3.827768782309125 1104 8 6.473644297395037 1104 9 6.586006962703689 1104 10-11 7.083607280182861 1104 12-13 7.58607502489312 1104 14-15 7.2174603631085965 1104 16-17 7.309346355248383 1104 18-19 6.904910679342965 1104 20-21 7.523658550219807 1104 22-23 6.395217823370945 1104 24-25 5.986665018103874 1104 26-27 8.228406632930323 1104 28-29 7.202276137784619 1104 30-31 6.4009684257757264 1104 32-33 7.55800902415346 1104 34-35 7.87690754784245 1104 36-37 6.460503044276546 1104 38-39 7.302322849525417 1104 40-41 7.1316101951306905 1104 42-43 7.313487159213821 1104 44-45 6.77079903254382 1104 46-47 6.649355905126864 1104 48-49 5.607096479419138 1104 50-51 5.776950780150205 1104 52-53 5.954858646838641 1104 54-55 4.822653136937216 1104 56-57 5.968972225711546 1104 58-59 3.942286301148073 1104 60-61 4.304031378635475 1104 62-63 3.8344622433102877 1104 64-65 2.721732040206902 1104 66-67 3.1903784601851832 1104 68-69 4.1554371430311186 1104 70-71 2.5441201311394295 1104 72-73 2.3628452316423143 1104 74-75 3.1713171028754097 1104 76-77 3.7642276578981964 1104 78-79 4.3481289103694145 1104 80-81 3.069670531394568 1104 82-83 2.9199202913800413 1104 84-85 2.8717243960930112 1104 86-87 0.6777149414979604 1104 88-89 0.24636982306098787 1104 90-91 2.1805665473221296 1104 92-93 2.3459757960706966 1104 94-95 2.4226695407504195 1104 96-97 2.8227071824086067 1104 98-99 2.5050848462688187 1104 100 0.9323845783124582 1105 1 5.844189255288683 1105 2 -2.385528980934872 1105 3 0.708376111094644 1105 4 8.58323008596646 1105 5 8.725120553498105 1105 6 8.070414610944653 1105 7 5.1822810181868455 1105 8 5.961809224988407 1105 9 4.831381981847059 1105 10-11 4.990809560079732 1105 12-13 5.035576397108045 1105 14-15 5.800852209902981 1105 16-17 6.018247499324463 1105 18-19 6.202299388360235 1105 20-21 7.101965094404889 1105 22-23 1.9927837765475722 1105 24-25 3.979522749705243 1105 26-27 6.855588022602198 1105 28-29 5.968366503759608 1105 30-31 5.330047873567079 1105 32-33 7.143331937320152 1105 34-35 6.059845084320941 1105 36-37 6.174497617652921 1105 38-39 4.189164386637582 1105 40-41 5.344829569624942 1105 42-43 5.59173211028201 1105 44-45 6.271972799328108 1105 46-47 5.486043787343338 1105 48-49 5.057757100959268 1105 50-51 5.981566714919623 1105 52-53 5.682330154235665 1105 54-55 5.362397259327238 1105 56-57 5.2540661642158994 1105 58-59 1.9681959536896017 1105 60-61 4.427755812830686 1105 62-63 -0.7974563899963094 1105 64-65 -0.5216446487657329 1105 66-67 0.6572492585284202 1105 68-69 2.6473229121021937 1105 70-71 2.892183297645424 1105 72-73 2.654016739155068 1105 74-75 2.7323477807481638 1105 76-77 1.717138447019181 1105 78-79 3.3185692805813396 1105 80-81 1.6091477845678632 1105 82-83 0.8811973674377676 1105 84-85 1.2749679616375396 1105 86-87 -0.42740785704638284 1105 88-89 1.4575441517699481 1105 90-91 1.2648198747970412 1105 92-93 1.6618055357221202 1105 94-95 1.0574327216452915 1105 96-97 2.0933745984796275 1105 98-99 2.050793509797195 1105 100 2.2081424147507143 1106 1 6.164630027453303 1106 2 10.246238581664585 1106 3 6.737516079278283 1106 4 11.570043815375993 1106 5 9.789733033763259 1106 6 9.265129445455205 1106 7 6.388152984272114 1106 8 7.229845410523868 1106 9 6.893588559618063 1106 10-11 5.44752263960234 1106 12-13 7.777847269295947 1106 14-15 7.64016457895875 1106 16-17 7.638484650780018 1106 18-19 7.064417326958722 1106 20-21 8.061740379753346 1106 22-23 6.045388311995847 1106 24-25 4.79573948244504 1106 26-27 7.497551583919375 1106 28-29 6.022342158001418 1106 30-31 5.206889363968411 1106 32-33 7.218933506934047 1106 34-35 6.577894303185879 1106 36-37 5.7806187709722465 1106 38-39 7.03390082639816 1106 40-41 6.426817433088782 1106 42-43 5.8856686294456395 1106 44-45 4.091491287983221 1106 46-47 5.708075553076256 1106 48-49 5.693890402845515 1106 50-51 6.4715039819958555 1106 52-53 5.37177388421415 1106 54-55 5.1326978354755965 1106 56-57 4.638910720457346 1106 58-59 4.7706511321948355 1106 60-61 4.697042320753074 1106 62-63 4.886715834424589 1106 64-65 5.077349278454651 1106 66-67 2.9392896813998064 1106 68-69 3.123029400018414 1106 70-71 2.705896929330052 1106 72-73 2.6224501530026316 1106 74-75 2.7721994414073876 1106 76-77 3.3050205084206965 1106 78-79 3.964090026007579 1106 80-81 3.4853285728888004 1106 82-83 1.9608290555722476 1106 84-85 2.424706994278761 1106 86-87 3.189220829161421 1106 88-89 3.3895593100334196 1106 90-91 3.2944288924574536 1106 92-93 2.991815741821668 1106 94-95 1.3424021597408604 1106 96-97 1.1980168198000953 1106 98-99 1.8455454015724406 1106 100 2.805038878231951 1107 1 5.878568925202334 1107 2 10.237627783178919 1107 3 5.882150935830413 1107 4 11.101688826803159 1107 5 9.104978470855986 1107 6 7.661999845918562 1107 7 3.336133771023924 1107 8 6.002544197829458 1107 9 5.359299905373071 1107 10-11 4.218238071951994 1107 12-13 5.747041575598061 1107 14-15 5.687776545747589 1107 16-17 6.683136458615454 1107 18-19 6.0015749908845315 1107 20-21 5.292235030856126 1107 22-23 5.564991322607543 1107 24-25 4.342752648390185 1107 26-27 6.836825365431281 1107 28-29 6.0654023788554134 1107 30-31 4.744815157209491 1107 32-33 4.635042704589747 1107 34-35 6.235547279424139 1107 36-37 6.099527244854986 1107 38-39 6.140398795906478 1107 40-41 7.345952462218133 1107 42-43 5.852196297582363 1107 44-45 3.824738529525604 1107 46-47 3.0841971243715243 1107 48-49 3.5194211382078286 1107 50-51 4.453656253848994 1107 52-53 3.4440283306498642 1107 54-55 4.9138856680019 1107 56-57 4.821362248232862 1107 58-59 2.526381445748232 1107 60-61 2.486112151220805 1107 62-63 0.2274859578326236 1107 64-65 1.7276644093883675 1107 66-67 3.7563838896266404 1107 68-69 4.647807833740767 1107 70-71 3.8878576571629466 1107 72-73 1.8901694759259229 1107 74-75 2.1863852638463293 1107 76-77 2.7342373905236244 1107 78-79 3.497068755136688 1107 80-81 1.955806981253982 1107 82-83 1.2948347349995686 1107 84-85 1.9660664438493676 1107 86-87 1.9999138476493314 1107 88-89 2.3214987740210837 1107 90-91 1.0734272589656229 1107 92-93 1.8714933580108308 1107 94-95 2.0217913574683095 1107 96-97 2.091360237203723 1107 98-99 1.5334121468468176 1107 100 -0.11569862516689966 1108 1 4.168199213628164 1108 2 -3.314359629514115 1108 3 -0.06173631265593116 1108 4 6.090922934610372 1108 5 6.573696404210917 1108 6 6.197775870743666 1108 7 3.7492062709911593 1108 8 4.632170104913815 1108 9 4.4452581053781905 1108 10-11 2.8973006895601188 1108 12-13 1.898868551305334 1108 14-15 3.286547048959015 1108 16-17 4.079500893856167 1108 18-19 3.3619431234282224 1108 20-21 4.172156004437639 1108 22-23 3.6173217905934756 1108 24-25 2.557084840203064 1108 26-27 4.31670007903697 1108 28-29 5.751278282038999 1108 30-31 4.030474901731093 1108 32-33 3.8668814637339217 1108 34-35 2.3751879594932177 1108 36-37 0.5506714427917636 1108 38-39 1.842149722260622 1108 40-41 4.695765031924839 1108 42-43 2.48528147972322 1108 44-45 3.0048039251039427 1108 46-47 4.002690978947186 1108 48-49 4.1752380680358385 1108 50-51 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-16.990784624588798 2213 46-47 -16.769981768507417 2213 48-49 -6.079115727097591 2213 50-51 -23.222563547193165 2213 52-53 -17.65475167528499 2213 54-55 -17.49116523437883 2213 56-57 -17.361290855033477 2213 58-59 -17.865187467589777 2213 60-61 -19.18328785751135 2213 62-63 -17.30260564165009 2213 64-65 -17.492756185295967 2213 66-67 -8.664886990079182 2213 68-69 -22.56133475271115 2213 70-71 -17.252493007857595 2213 72-73 -9.331900081630558 2213 74-75 -4.1362988720492275 2213 76-77 -2.944068083614951 2213 78-79 -4.300753576672484 2213 80-81 -3.314791355140727 2213 82-83 -3.2465412271004226 2213 84-85 -3.0008083528359393 2213 86-87 -3.230532155691982 2213 88-89 -2.6676659065586783 2213 90-91 -2.6267022133341413 2213 92-93 -3.466399007318742 2213 94-95 -3.5771915104951546 2213 96-97 -3.271724941002269 2213 98-99 -3.527157849674669 2213 100 -3.2366973868817723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 817.0 3 749.0 4 3695.0 5 13612.0 6 19784.0 7 22938.0 8 20658.0 9 21414.0 10 26616.0 11 33580.0 12 43056.0 13 55309.0 14 48083.0 15 54358.0 16 60674.0 17 62678.0 18 63340.0 19 63755.0 20 64003.0 21 63095.0 22 66503.0 23 72376.0 24 78803.0 25 86106.0 26 92665.0 27 99162.0 28 105889.0 29 111053.0 30 117999.0 31 128696.0 32 145279.0 33 171594.0 34 200685.0 35 223820.0 36 227421.0 37 177279.0 38 48133.0 39 82.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.21456676971055 20.98187644363134 13.931413305984345 23.87214348067377 2 14.86815377868199 21.591875237024734 39.79984730016562 23.74012368412766 3 17.626552307187833 26.958109917729324 30.33463337247495 25.080704402607896 4 12.17469090902572 18.112571992752212 39.20201711916052 30.510719979061545 5 12.999152957328258 38.22610686277751 35.643650092655136 13.131090087239098 6 30.430124691547576 38.082335510136595 18.70898968567855 12.778550112637282 7 26.593341158240698 31.845978328841856 24.039213693936542 17.521466818980905 8 23.820717631740596 36.574882172631476 22.115270404336695 17.489129791291237 9 24.65910859441405 17.360856412226564 22.237579733599176 35.74245525976021 10-11 23.03842715721654 27.642841862416496 29.217478746278207 20.101252234088758 12-13 24.13853379482705 25.288679009290437 28.99643858325151 21.576348612630998 14-15 21.618365613540774 26.691342238346323 26.514852736460803 25.1754394116521 16-17 21.02787008990135 30.258456109909453 27.57328703250888 21.14038676768031 18-19 21.069608900836847 29.38135042402483 28.910770554961108 20.638270120177214 20-21 22.420988650624892 28.78115585588627 27.846543124659977 20.95131236882886 22-23 21.263154127649038 28.87517487407788 28.382076534500634 21.479594463772443 24-25 21.636660017603447 28.76302753205591 27.817785433835045 21.782527016505593 26-27 21.61580258201542 28.643703777780953 27.98390407207928 21.756589568124348 28-29 21.40472426009053 28.501554424715813 28.576399138477054 21.517322176716604 30-31 22.13060654038174 28.99728199296098 27.746097420927683 21.126014045729598 32-33 21.048494720211476 28.92781416228284 28.501223507911217 21.52246760959447 34-35 21.134013087483595 28.48418695908437 28.326765795887702 22.055034157544334 36-37 21.874494162508455 28.98315705348565 27.83088577970264 21.311463004303253 38-39 21.687613135567066 28.65131506044917 28.012874674135684 21.648197129848082 40-41 21.687430497199173 28.669425971014427 27.678196363712367 21.96494716807403 42-43 21.528180269904862 28.684767553328864 28.27230924630496 21.51474293046131 44-45 22.047165188483316 28.196971800362046 28.096466977840578 21.659396033314056 46-47 21.794463312879223 28.704449102059904 28.295652964598204 21.20543462046267 48-49 21.65916637026012 28.484570626640217 28.32281605771937 21.533446945380298 50-51 21.908898472808147 28.4225168669454 28.020507725458664 21.648076934787788 52-53 21.269165147425596 28.613597709760946 28.125186063934944 21.992051078878518 54-55 21.814345884759078 28.54114445397465 27.79584780341612 21.84866185785015 56-57 21.930745849776525 28.42328178815209 27.817290812442668 21.82868154962872 58-59 21.62408551626082 27.97283879210699 28.32121781344039 22.081857878191798 60-61 22.151153013607445 28.47340312788671 27.741495370411084 21.633948488094763 62-63 21.496194255241402 28.602023388370895 28.19823480302681 21.703547553360888 64-65 21.492422244533728 28.066213950689484 28.248023080505785 22.193340724271003 66-67 21.72606941737537 28.3802494615144 27.903961380996893 21.989719740113333 68-69 22.24339722907779 28.148502677648562 27.804164892423245 21.803935200850404 70-71 22.203825244180127 28.247691964612304 27.648555205011643 21.89992758619593 72-73 21.97164451708492 28.308455061416556 28.083145385453186 21.636755036045344 74-75 22.182088624880635 27.92610519460719 27.745929932267384 22.145876248244793 76-77 22.006087376416154 28.377903183106962 27.927100168422882 21.688909272053998 78-79 22.108979078831563 28.102982759969397 28.08830240754272 21.69973575365632 80-81 22.14932028392938 28.086885866208544 27.826869995406934 21.936923854455145 82-83 21.5408093444283 28.264787558480077 28.23205367383067 21.962349423260953 84-85 21.808360649035766 27.98578011095399 27.72051991906096 22.485339320949283 86-87 22.103022884424504 28.16118145624123 27.778676923162234 21.957118736172028 88-89 21.855816665522507 27.760101705725987 28.217760093015897 22.166321535735605 90-91 22.21528015040591 27.836263001160393 27.84476549987333 22.103691348560368 92-93 21.63597138128351 28.174054176709575 28.139757445898205 22.050216996108706 94-95 21.57411342391786 27.76612900286969 28.106545481616347 22.5532120915961 96-97 21.87109775720304 27.94011917842221 28.04704301775179 22.14174004662296 98-99 21.82797902949688 27.645625410989123 28.072152348291258 22.454243211222742 100 21.92067674386354 27.607786714741366 27.88739425877132 22.58414228262377 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 336.0 1 382.0 2 357.0 3 282.0 4 339.0 5 463.5 6 642.5 7 937.0 8 1409.0 9 2037.5 10 2792.5 11 3607.5 12 4451.0 13 5313.5 14 6132.5 15 6944.5 16 7790.0 17 8583.0 18 9264.0 19 9960.0 20 10840.5 21 11890.5 22 13113.0 23 14643.5 24 16751.0 25 19548.5 26 23424.0 27 28758.5 28 35591.5 29 43301.0 30 52087.5 31 61514.5 32 70421.5 33 79235.0 34 87386.0 35 94693.5 36 100670.5 37 105488.5 38 109763.0 39 112392.5 40 114003.5 41 113959.5 42 111617.0 43 108627.0 44 104706.5 45 99567.0 46 94634.5 47 90277.5 48 86994.0 49 85923.0 50 86583.5 51 82389.0 52 72422.5 53 63510.0 54 56831.5 55 51539.5 56 46805.0 57 42265.0 58 37687.0 59 33131.0 60 28608.5 61 24049.5 62 20049.5 63 16429.5 64 13147.5 65 10506.0 66 8337.5 67 6594.5 68 5163.0 69 3906.5 70 2994.0 71 2352.0 72 1783.5 73 1308.5 74 994.0 75 764.5 76 544.5 77 412.0 78 314.5 79 210.0 80 136.5 81 92.5 82 62.5 83 40.5 84 29.0 85 20.5 86 17.5 87 14.0 88 7.0 89 3.5 90 3.5 91 3.5 92 1.5 93 1.0 94 0.5 95 1.0 96 1.5 97 2.0 98 2.0 99 1.0 100 2.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.470165852890383 2 24.329787112808766 3 5.744746023408716 4 22.945901230040207 5 12.264660146096412 6 9.357201341686238 7 7.557155136183639 8 8.418725453326745 9 4.808273064160381 10-11 6.453592995825964 12-13 9.715932852146882 14-15 7.097068506046256 16-17 7.495271533300942 18-19 6.277007168068889 20-21 7.419902692178458 22-23 5.434153878136958 24-25 4.816733712992 26-27 9.35407608160762 28-29 6.059205893860643 30-31 3.8331228531103587 32-33 8.241189960904896 34-35 6.636722876454843 36-37 6.449483537822036 38-39 5.729188789536698 40-41 7.4750868425169354 42-43 6.068409007793812 44-45 6.094654285802099 46-47 5.433894878682929 48-49 3.73658512327856 50-51 6.431595308863755 52-53 4.010572012380865 54-55 4.499096782570649 56-57 4.416665889668304 58-59 3.575625595914577 60-61 3.792459938827782 62-63 2.897047026358202 64-65 4.278532847519425 66-67 2.28140532413091 68-69 4.830840549921454 70-71 2.2856874484375256 72-73 1.4549207996936209 74-75 0.6792865013973884 76-77 1.0584271688355282 78-79 1.5543420567802775 80-81 1.8451984436550142 82-83 0.7273395334349302 84-85 1.1690199357059754 86-87 0.35579618331497886 88-89 0.6503130958066607 90-91 0.28912972384787544 92-93 0.31723979792517265 94-95 0.4985048824850411 96-97 0.5250436932078947 98-99 0.4306642921596721 100 0.39226330644228335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2895759.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.9162267844431 #Duplication Level Percentage of deduplicated Percentage of total 1 96.90883661168024 91.98221113250786 2 2.502169566633285 4.749929880793932 3 0.3441593947558774 0.9799893348793662 4 0.10908305782744848 0.4141500902036248 5 0.04719508773649711 0.22397898253545245 6 0.023516144976756602 0.13392382498258032 7 0.015214253561665506 0.1010855678990545 8 0.010892350884973109 0.08270886774510697 9 0.006520882765299916 0.0557043828647376 >10 0.029458256333862662 0.4682876717740693 >50 0.0014122465851704811 0.09386198141344815 >100 0.0011392806517217845 0.25193454368302576 >500 2.1974487685830254E-4 0.13379992949618563 >1k 1.831207303320345E-4 0.32843380922144716 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3464 0.11962321450093048 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.4533260537220124E-5 0.0 3.4533260537220124E-5 0.0 5 0.0 3.4533260537220124E-5 0.0 3.4533260537220124E-5 0.0 6 0.0 3.4533260537220124E-5 0.0 3.4533260537220124E-5 0.0 7 0.0 3.4533260537220124E-5 0.0 6.906652107444025E-5 0.0 8 0.0 3.4533260537220124E-5 0.0 6.906652107444025E-5 0.0 9 0.0 3.4533260537220124E-5 0.0 6.906652107444025E-5 0.0 10-11 0.0 3.4533260537220124E-5 0.0 1.0359978161166036E-4 0.0 12-13 0.0 3.4533260537220124E-5 0.0 1.2086641188027043E-4 0.0 14-15 0.0 3.4533260537220124E-5 0.0 1.8993293295471066E-4 0.0 16-17 3.4533260537220124E-5 3.4533260537220124E-5 0.0 2.0719956322332072E-4 0.0 18-19 3.4533260537220124E-5 3.4533260537220124E-5 0.0 2.0719956322332072E-4 0.0 20-21 3.4533260537220124E-5 3.4533260537220124E-5 0.0 2.93532714566371E-4 0.0 22-23 3.4533260537220124E-5 3.4533260537220124E-5 0.0 3.107993448349811E-4 0.0 24-25 3.4533260537220124E-5 3.4533260537220124E-5 0.0 3.798658659094213E-4 0.0 26-27 3.4533260537220124E-5 3.4533260537220124E-5 0.0 4.4893238698386154E-4 0.0 28-29 3.4533260537220124E-5 3.4533260537220124E-5 0.0 6.215986896699622E-4 0.0 30-31 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.0018302628084726665 1.7266630268610062E-5 32-33 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.006026053963744911 3.4533260537220124E-5 34-35 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.011724041952386232 3.4533260537220124E-5 36-37 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.021082555557972886 3.4533260537220124E-5 38-39 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.03663978942999055 3.4533260537220124E-5 40-41 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.056306481305937406 3.4533260537220124E-5 42-43 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.0760422397029587 3.4533260537220124E-5 44-45 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.099542123498537 3.4533260537220124E-5 46-47 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.12670253291106062 3.4533260537220124E-5 48-49 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.15603853773742912 3.4533260537220124E-5 50-51 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.18893146839913127 3.4533260537220124E-5 52-53 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.22595112369503123 3.4533260537220124E-5 54-55 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.2675982359029187 3.4533260537220124E-5 56-57 3.4533260537220124E-5 3.4533260537220124E-5 0.0 0.31535773522589416 3.4533260537220124E-5 58-59 6.906652107444025E-5 3.4533260537220124E-5 0.0 0.36878068927697366 3.4533260537220124E-5 60-61 6.906652107444025E-5 3.4533260537220124E-5 3.4533260537220124E-5 0.4256569693817752 3.4533260537220124E-5 62-63 6.906652107444025E-5 3.4533260537220124E-5 3.4533260537220124E-5 0.48514051065713687 3.4533260537220124E-5 64-65 6.906652107444025E-5 3.4533260537220124E-5 3.4533260537220124E-5 0.5558473616070951 3.4533260537220124E-5 66-67 6.906652107444025E-5 3.4533260537220124E-5 3.4533260537220124E-5 0.6391243193926014 3.4533260537220124E-5 68-69 6.906652107444025E-5 3.4533260537220124E-5 3.4533260537220124E-5 0.7338317864159276 3.4533260537220124E-5 70-71 6.906652107444025E-5 3.4533260537220124E-5 3.4533260537220124E-5 0.8442000870928831 3.4533260537220124E-5 72-73 6.906652107444025E-5 3.4533260537220124E-5 3.4533260537220124E-5 0.9608534411876126 6.906652107444025E-5 74-75 8.63331513430503E-5 3.4533260537220124E-5 3.4533260537220124E-5 1.0839299817422652 6.906652107444025E-5 76-77 1.0359978161166036E-4 3.4533260537220124E-5 3.4533260537220124E-5 1.2257753493988968 6.906652107444025E-5 78-79 1.0359978161166036E-4 3.4533260537220124E-5 3.4533260537220124E-5 1.3790512262933483 6.906652107444025E-5 80-81 1.0359978161166036E-4 3.4533260537220124E-5 3.4533260537220124E-5 1.5519074619124038 6.906652107444025E-5 82-83 1.0359978161166036E-4 3.4533260537220124E-5 3.4533260537220124E-5 1.7499384444630923 6.906652107444025E-5 84-85 1.0359978161166036E-4 3.4533260537220124E-5 3.4533260537220124E-5 1.952268817950665 6.906652107444025E-5 86-87 1.0359978161166036E-4 6.906652107444025E-5 3.4533260537220124E-5 2.172988152674307 6.906652107444025E-5 88 1.0359978161166036E-4 6.906652107444025E-5 3.4533260537220124E-5 2.359830358810937 6.906652107444025E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGA 1230 0.0 19.009539 1 GTACATG 13470 0.0 15.622539 1 TACATGG 13590 0.0 15.392493 2 TATACTG 1390 0.0 15.157512 5 GTATTAG 1270 0.0 15.019348 1 CCTATAC 785 0.0 14.913598 3 GTATTAT 1645 0.0 14.587866 1 CATGGGG 3780 0.0 14.308097 4 TGTACCG 250 9.93599E-4 14.04596 5 ACATGGG 13645 0.0 13.972863 3 CAGCGTA 360 5.4613392E-6 13.951608 9 GTGCTAG 885 1.8189894E-12 13.210019 1 GTATAAG 1125 0.0 13.126586 1 GTATTAC 915 5.456968E-12 12.776902 1 TTATACT 1700 0.0 12.463402 4 GTCTCGC 395 4.6956027E-4 12.461949 1 TCTAGAC 985 0.0 12.45143 3 GTGTACA 1345 0.0 12.351923 1 GTATATA 1850 0.0 12.306175 1 GTCTAGT 800 1.1696102E-9 12.306174 1 >>END_MODULE