FastQCFastQC Report
Wed 25 May 2016
SRR1295107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895759
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94330.3257522466475974No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72600.25071147150021805No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31540.10891790373439227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG1250.00644627118.7833949
CATGGGG42200.016.6007794
CTATGCG1700.001930959216.5735849
GAGTACT33100.016.38609912-13
GTATATA20500.016.1612951
CTAGGAC11450.015.6030753
GTATTAG12150.015.4654461
GTATAAG10700.014.7884061
GTCTATA10650.013.9292211
GTACATG172500.013.7596481
GTATAGG9000.013.735761
TAGTACG3103.8528902E-513.6500884
TAGGACA14350.013.433424
ACATGGG164950.013.4245793
CATGGGT21050.013.4015114
TACATGG175800.013.3752232
AGTACTT40900.013.26112112-13
AACCGTC2903.3875796E-412.9540657
GTATAGC6902.0008883E-1112.899671
TATACTG16050.012.8893875
Produced by FastQC (version 0.11.5)