##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295107_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2895759 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.557276693260732 33.0 33.0 33.0 27.0 33.0 2 30.328070119094857 33.0 33.0 33.0 27.0 33.0 3 31.09305297851099 33.0 33.0 33.0 27.0 33.0 4 35.331695420786055 37.0 37.0 37.0 33.0 37.0 5 35.62876641322707 37.0 37.0 37.0 33.0 37.0 6 35.451851138164464 37.0 37.0 37.0 33.0 37.0 7 35.355056135541666 37.0 37.0 37.0 33.0 37.0 8 35.355025746272396 37.0 37.0 37.0 33.0 37.0 9 35.307635752837164 37.0 37.0 37.0 33.0 37.0 10-11 35.292694592333135 37.0 37.0 37.0 33.0 37.0 12-13 35.22175395120934 37.0 37.0 37.0 33.0 37.0 14-15 36.733793454496734 40.0 37.0 40.0 33.0 40.0 16-17 36.72529948106869 40.0 37.0 40.0 33.0 40.0 18-19 36.74247304420015 40.0 37.0 40.0 33.0 40.0 20-21 36.74451827655547 40.0 37.0 40.0 33.0 40.0 22-23 36.708923463589336 40.0 37.0 40.0 33.0 40.0 24-25 36.69187266619909 40.0 37.0 40.0 33.0 40.0 26-27 36.67634444026592 40.0 37.0 40.0 33.0 40.0 28-29 36.623221407582605 40.0 37.0 40.0 33.0 40.0 30-31 36.53854619807795 40.0 37.0 40.0 33.0 40.0 32-33 36.50057756878249 40.0 37.0 40.0 33.0 40.0 34-35 36.419234646253365 37.0 37.0 40.0 33.0 40.0 36-37 36.40617537578231 37.0 37.0 40.0 33.0 40.0 38-39 36.34710606096709 37.0 37.0 40.0 33.0 40.0 40-41 36.28474123709881 37.0 37.0 40.0 33.0 40.0 42-43 36.20551192278087 37.0 37.0 40.0 30.0 40.0 44-45 36.111040663259615 37.0 37.0 40.0 27.0 40.0 46-47 36.00892373985542 37.0 37.0 40.0 27.0 40.0 48-49 35.89585580153597 37.0 37.0 40.0 27.0 40.0 50-51 35.7926864770169 37.0 37.0 40.0 27.0 40.0 52-53 35.69898444587412 37.0 37.0 40.0 27.0 40.0 54-55 35.58537675269247 37.0 37.0 40.0 27.0 40.0 56-57 35.45965202905352 37.0 35.0 40.0 27.0 40.0 58-59 35.32976915551329 37.0 33.0 40.0 27.0 40.0 60-61 35.16521333439695 37.0 33.0 40.0 27.0 40.0 62-63 34.980171001799526 37.0 33.0 40.0 27.0 40.0 64-65 34.796991738608085 37.0 33.0 40.0 27.0 40.0 66-67 34.57351578636206 37.0 33.0 40.0 27.0 40.0 68-69 34.35915885955979 37.0 33.0 38.5 27.0 40.0 70-71 34.120407637514035 37.0 33.0 37.0 27.0 40.0 72-73 33.891262359885616 37.0 33.0 37.0 27.0 40.0 74-75 33.64624076105781 37.0 33.0 37.0 27.0 40.0 76-77 33.40342480158052 37.0 33.0 37.0 27.0 38.5 78-79 33.16880099483417 37.0 33.0 37.0 27.0 37.0 80-81 32.952389684362544 37.0 33.0 37.0 27.0 37.0 82-83 32.75069420486995 37.0 33.0 37.0 22.0 37.0 84-85 32.5755047295027 37.0 33.0 37.0 22.0 37.0 86-87 32.597273633613845 35.0 33.0 37.0 24.5 37.0 88-89 32.60695468787285 37.0 33.0 37.0 22.0 37.0 90-91 32.616679254040136 37.0 33.0 37.0 27.0 37.0 92-93 32.57942131924653 33.0 33.0 37.0 22.0 37.0 94-95 32.61895499590953 35.0 33.0 37.0 27.0 37.0 96-97 32.58158759068003 33.0 33.0 37.0 22.0 37.0 98-99 32.50969607622734 33.0 33.0 37.0 22.0 37.0 100 31.973475693246574 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.538013712479696 1101 2 4.038372149146667 1101 3 2.279889397175399 1101 4 1.035151608501181 1101 5 0.48924121237708107 1101 6 0.46547786179548467 1101 7 0.4905158146642208 1101 8 0.46906147181400115 1101 9 0.515511362194637 1101 10-11 0.5103453886875542 1101 12-13 0.5528615155859171 1101 14-15 0.8764881099535131 1101 16-17 0.8852049571981695 1101 18-19 0.8980822397017434 1101 20-21 0.870463749822882 1101 22-23 0.8055824752287464 1101 24-25 0.8584833781650119 1101 26-27 0.9233532202374093 1101 28-29 0.759280520951954 1101 30-31 0.7049165940843665 1101 32-33 0.8994737962046528 1101 34-35 0.9552960564521129 1101 36-37 0.8464604865144736 1101 38-39 0.8489894090978467 1101 40-41 0.9023208329037189 1101 42-43 0.9733983518062885 1101 44-45 0.9653239024318907 1101 46-47 0.8889283827824457 1101 48-49 0.8589571386950823 1101 50-51 0.8739978861777047 1101 52-53 0.8830683937402952 1101 54-55 0.8391104960727844 1101 56-57 0.8028082535281413 1101 58-59 0.9009251473851592 1101 60-61 0.8080063270680355 1101 62-63 0.8421758799690551 1101 64-65 0.7387641581890776 1101 66-67 0.8066389615894778 1101 68-69 0.7951075138189765 1101 70-71 0.8259505170287014 1101 72-73 0.7409696806410082 1101 74-75 0.6743783456164465 1101 76-77 0.7068902847265051 1101 78-79 0.7601049149747681 1101 80-81 0.7456698809639875 1101 82-83 0.7784943837991491 1101 84-85 0.776180203867419 1101 86-87 0.7414334067345152 1101 88-89 0.7678833642667584 1101 90-91 0.8183664406507916 1101 92-93 0.8697772600428095 1101 94-95 0.8432678424364539 1101 96-97 0.7573457712785157 1101 98-99 0.7491280841212031 1101 100 0.8257483458475043 1102 1 0.5500985973886898 1102 2 4.055546892160578 1102 3 2.322077829179225 1102 4 1.1050915274774127 1102 5 0.48430470859214836 1102 6 0.5086362833937983 1102 7 0.4998821884837241 1102 8 0.43617261512220296 1102 9 0.5241956385786324 1102 10-11 0.5642310860921924 1102 12-13 0.623228568680382 1102 14-15 0.8373448901901881 1102 16-17 0.8085188593788075 1102 18-19 0.8882148770761589 1102 20-21 0.8514068882990173 1102 22-23 0.8058781291861337 1102 24-25 0.8236027969615449 1102 26-27 0.8407552193003482 1102 28-29 0.8846719037563204 1102 30-31 0.8118372487473735 1102 32-33 0.956972022751323 1102 34-35 0.967548643247838 1102 36-37 0.8729602070897258 1102 38-39 0.8271945143854609 1102 40-41 0.8618670452401744 1102 42-43 0.8905656152690185 1102 44-45 0.9347492376945397 1102 46-47 0.9617204098631191 1102 48-49 0.8618727524228262 1102 50-51 0.8590949980360421 1102 52-53 0.8365942729874121 1102 54-55 0.8281760037696699 1102 56-57 0.8093928458408541 1102 58-59 0.8259567590732004 1102 60-61 0.7891458625643892 1102 62-63 0.8463404376218122 1102 64-65 0.847041951542721 1102 66-67 0.8231064401245831 1102 68-69 0.7960088232578855 1102 70-71 0.8231635609896486 1102 72-73 0.8167217753857585 1102 74-75 0.7839716461965622 1102 76-77 0.7240469168774766 1102 78-79 0.7000518807231302 1102 80-81 0.7101156697414552 1102 82-83 0.7873971665685175 1102 84-85 0.7534294319212833 1102 86-87 0.8088091097652779 1102 88-89 0.7603671209395486 1102 90-91 0.787916905511949 1102 92-93 0.9400982127588833 1102 94-95 0.8255210784007616 1102 96-97 0.8323922479865402 1102 98-99 0.8065068260136457 1102 100 0.8372480381980445 1103 1 0.5019787455476994 1103 2 3.9979909477919193 1103 3 2.2782448029661673 1103 4 0.9287237477918282 1103 5 0.41867356232581443 1103 6 0.37144829516723377 1103 7 0.38829701276446116 1103 8 0.3866975128136829 1103 9 0.4539777309016486 1103 10-11 0.4940618248165052 1103 12-13 0.5250554978778084 1103 14-15 0.6401401345663444 1103 16-17 0.6755927692764061 1103 18-19 0.7126904527554601 1103 20-21 0.6535398164538222 1103 22-23 0.5995010089886321 1103 24-25 0.6200297943243669 1103 26-27 0.6806619269844276 1103 28-29 0.6654986145988957 1103 30-31 0.6213050067586252 1103 32-33 0.6668938339847514 1103 34-35 0.8966304993286087 1103 36-37 0.7498505176128987 1103 38-39 0.6574616304656473 1103 40-41 0.6229236773057849 1103 42-43 0.7055165680133157 1103 44-45 0.7419973903398969 1103 46-47 0.7266957462383843 1103 48-49 0.6372205490127953 1103 50-51 0.6520594895874794 1103 52-53 0.6484942503461824 1103 54-55 0.6522946377384358 1103 56-57 0.6199717158584761 1103 58-59 0.6077016077919595 1103 60-61 0.5944388428181782 1103 62-63 0.6643150713939789 1103 64-65 0.6521908055019949 1103 66-67 0.6189020918010399 1103 68-69 0.6036197592106163 1103 70-71 0.7371153790960179 1103 72-73 0.7122017592548886 1103 74-75 0.6795991303859807 1103 76-77 0.5980801325999892 1103 78-79 0.5985024676626054 1103 80-81 0.6181887263630514 1103 82-83 0.6717156214218463 1103 84-85 0.60874345199543 1103 86-87 0.5865165589695778 1103 88-89 0.5930687225039719 1103 90-91 0.5860174196530465 1103 92-93 0.6998373633020663 1103 94-95 0.6286122802307617 1103 96-97 0.6454527894904842 1103 98-99 0.711347414878837 1103 100 0.5976766986030562 1104 1 0.4344262251205464 1104 2 3.6363876242769493 1104 3 2.1414422991498014 1104 4 0.9209553251953153 1104 5 0.3542659317727441 1104 6 0.4022960942843028 1104 7 0.2619595873150047 1104 8 0.3762611561937774 1104 9 0.3766045072015629 1104 10-11 0.37954161118636165 1104 12-13 0.3196454829826152 1104 14-15 0.4926893194652777 1104 16-17 0.5336719961455998 1104 18-19 0.5122255538673173 1104 20-21 0.5223095372630056 1104 22-23 0.4776997296392196 1104 24-25 0.482186699299767 1104 26-27 0.4387450031334197 1104 28-29 0.4737338980077155 1104 30-31 0.4655267363827349 1104 32-33 0.5725077707123631 1104 34-35 0.6036100664155128 1104 36-37 0.48246027119933643 1104 38-39 0.47800114829822604 1104 40-41 0.43412090051711516 1104 42-43 0.5437816721552338 1104 44-45 0.6406798721210052 1104 46-47 0.5563361623082415 1104 48-49 0.4716247123855837 1104 50-51 0.43956935834889777 1104 52-53 0.4000705522770005 1104 54-55 0.4921616285365715 1104 56-57 0.5074460822778164 1104 58-59 0.46932313631756273 1104 60-61 0.4761476849404147 1104 62-63 0.515446888219067 1104 64-65 0.42368988167484645 1104 66-67 0.4425705058401732 1104 68-69 0.49398946800656063 1104 70-71 0.36750176090466624 1104 72-73 0.47446284677491946 1104 74-75 0.4156436269289827 1104 76-77 0.43168708107199194 1104 78-79 0.44538377449026 1104 80-81 0.39208592698781786 1104 82-83 0.4015321015437152 1104 84-85 0.43320893951224093 1104 86-87 0.4283242347574685 1104 88-89 0.4694397037993028 1104 90-91 0.4585586632589198 1104 92-93 0.4973619549801356 1104 94-95 0.5412656291607476 1104 96-97 0.4563375447791387 1104 98-99 0.3935272447967222 1104 100 0.342931876282929 1105 1 0.49814580901853844 1105 2 3.7242808220694243 1105 3 2.2021593021804655 1105 4 0.9175571082067506 1105 5 0.31936192252374695 1105 6 0.2633680485464609 1105 7 0.2554286080988888 1105 8 0.34834579259443643 1105 9 0.33969477403606163 1105 10-11 0.3260100946056639 1105 12-13 0.3183931238955111 1105 14-15 0.45024779145705907 1105 16-17 0.44511887761151314 1105 18-19 0.4398057814738152 1105 20-21 0.3626191719052443 1105 22-23 0.39943711073332366 1105 24-25 0.4461730495449743 1105 26-27 0.3958542954259272 1105 28-29 0.3975207868519348 1105 30-31 0.3745962778406309 1105 32-33 0.39674142182246186 1105 34-35 0.5564352166725115 1105 36-37 0.48089266851017953 1105 38-39 0.3944397211227013 1105 40-41 0.3765673791203241 1105 42-43 0.5107020407418119 1105 44-45 0.4985584354403372 1105 46-47 0.4304217777694248 1105 48-49 0.48635867319718784 1105 50-51 0.47997873783617706 1105 52-53 0.43446148700463283 1105 54-55 0.43903729517919743 1105 56-57 0.3657041954993403 1105 58-59 0.3339492448811541 1105 60-61 0.43201877556163737 1105 62-63 0.4318923018657088 1105 64-65 0.40465147495890363 1105 66-67 0.3732293220204639 1105 68-69 0.33257673278739475 1105 70-71 0.35223595188679013 1105 72-73 0.39232735811968666 1105 74-75 0.3948707503259641 1105 76-77 0.3375281273680244 1105 78-79 0.38636222808640497 1105 80-81 0.39553350848072455 1105 82-83 0.4728644275274121 1105 84-85 0.35917793490021666 1105 86-87 0.4208199557074721 1105 88-89 0.3352408392986277 1105 90-91 0.3098447699734308 1105 92-93 0.37895352112079195 1105 94-95 0.3819932611831476 1105 96-97 0.3580062167582483 1105 98-99 0.43787950959106325 1105 100 0.4449203309810663 1106 1 0.4881557870348203 1106 2 3.847505012129453 1106 3 2.204600996202501 1106 4 0.9054274145476384 1106 5 0.31738048048108425 1106 6 0.2151428997847873 1106 7 0.14517260593736125 1106 8 0.27764281239832656 1106 9 0.19573711330816224 1106 10-11 0.2083560738611112 1106 12-13 0.22353531125413895 1106 14-15 0.2892623489341659 1106 16-17 0.2184283983835087 1106 18-19 0.3498754070237524 1106 20-21 0.32275868868452307 1106 22-23 0.18381689092621656 1106 24-25 0.22021672272821746 1106 26-27 0.23806011375485525 1106 28-29 0.24827833659602305 1106 30-31 0.21105269350959333 1106 32-33 0.28019396834705645 1106 34-35 0.3749723708099282 1106 36-37 0.29162485088355083 1106 38-39 0.24019476571046994 1106 40-41 0.20017859640572766 1106 42-43 0.34862367834958974 1106 44-45 0.3337180097617676 1106 46-47 0.2074269743082766 1106 48-49 0.16575815502208968 1106 50-51 0.13816393192375642 1106 52-53 0.16129360343437327 1106 54-55 0.1897841926828292 1106 56-57 0.1940155321650323 1106 58-59 0.2215998769600347 1106 60-61 0.237029815042348 1106 62-63 0.23614467625010604 1106 64-65 0.1629076881601179 1106 66-67 0.06382534870971313 1106 68-69 0.09242786208780274 1106 70-71 0.07693421151121527 1106 72-73 0.10634706000232796 1106 74-75 0.04577716826324263 1106 76-77 0.22774953355901317 1106 78-79 0.24598715392802006 1106 80-81 0.1858461981062689 1106 82-83 0.202603465181447 1106 84-85 0.2061917108788336 1106 86-87 0.308751481100785 1106 88-89 0.2652230362718342 1106 90-91 0.22190994865160718 1106 92-93 0.3089378736061832 1106 94-95 0.26368682204165594 1106 96-97 0.25573984608183054 1106 98-99 0.2736193427794902 1106 100 0.20263978405243321 1107 1 0.45314697730041686 1107 2 3.538365798992878 1107 3 2.02914554755829 1107 4 0.8963343366056691 1107 5 0.30437869172705945 1107 6 0.18204527000968795 1107 7 0.09965726425365062 1107 8 0.09073839626017133 1107 9 0.12636284038343604 1107 10-11 0.1502703740125355 1107 12-13 0.1882904317300742 1107 14-15 0.17681707997903118 1107 16-17 0.03582117242315519 1107 18-19 0.18070453953311727 1107 20-21 0.16787455907687132 1107 22-23 0.16035413738507742 1107 24-25 0.168560302818328 1107 26-27 0.09396833855039688 1107 28-29 0.012420413064447189 1107 30-31 0.050274467667186684 1107 32-33 0.1364262955006481 1107 34-35 0.3330088760612142 1107 36-37 0.1268152142434218 1107 38-39 -0.005139346225895736 1107 40-41 0.061037871505668306 1107 42-43 0.26776599473696905 1107 44-45 0.2791178988559295 1107 46-47 0.11579275649365428 1107 48-49 0.10437573701071301 1107 50-51 0.07637471051832279 1107 52-53 0.10664063810831692 1107 54-55 0.09431799826038656 1107 56-57 0.09032060334423164 1107 58-59 0.08181049889724079 1107 60-61 0.12587259697216524 1107 62-63 0.18827675853687964 1107 64-65 0.05225762587834737 1107 66-67 0.07806412771505222 1107 68-69 0.10495980332314758 1107 70-71 0.017423572438367785 1107 72-73 0.06733299885802069 1107 74-75 -0.027567987618560608 1107 76-77 0.014228924420969236 1107 78-79 0.06378651959525428 1107 80-81 0.1445174920447556 1107 82-83 0.16349295063725577 1107 84-85 0.26128607227511935 1107 86-87 0.160292707523638 1107 88-89 0.09653965520136154 1107 90-91 0.05550308207639176 1107 92-93 0.17570347756890214 1107 94-95 0.17989426688384214 1107 96-97 0.15944591880184333 1107 98-99 0.08404237147155413 1107 100 0.1917473388487494 1108 1 0.3256501650071968 1108 2 3.555169129810995 1108 3 2.0397924070663933 1108 4 0.8565539279425991 1108 5 0.17564374478685352 1108 6 0.0014229073182434604 1108 7 -0.05062921431490963 1108 8 -0.09942133546692133 1108 9 -0.12959140892488108 1108 10-11 -0.061640371445882636 1108 12-13 -0.08573506617942428 1108 14-15 -0.19167066443522174 1108 16-17 -0.13442125556400697 1108 18-19 -0.12589276213472544 1108 20-21 -0.1432952403737815 1108 22-23 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0.004091865212799917 2211 74-75 -6.217926239386884E-4 2211 76-77 0.04534223768685308 2211 78-79 0.006136544469327987 2211 80-81 0.053523171245622336 2211 82-83 -0.38789742069705824 2211 84-85 -0.08876320841566354 2211 86-87 -0.006445346730274082 2211 88-89 0.022747323407450892 2211 90-91 0.04405215132931062 2211 92-93 0.18340967247519302 2211 94-95 0.038360094780415466 2211 96-97 -0.042608695356825876 2211 98-99 0.08559281402526153 2211 100 0.02465800786578143 2212 1 0.576477562964385 2212 2 4.048405638244446 2212 3 2.347858387577485 2212 4 1.160219935847735 2212 5 0.511188690585783 2212 6 0.5292987927328738 2212 7 0.44651329341412804 2212 8 0.31448916427870444 2212 9 0.23895357600839162 2212 10-11 0.2246911258342834 2212 12-13 0.3069026867263034 2212 14-15 0.3602122847292577 2212 16-17 0.4233447601679927 2212 18-19 0.2914981508696144 2212 20-21 0.45165814083390643 2212 22-23 0.31702517960772525 2212 24-25 0.34093620040334116 2212 26-27 0.24241298376100673 2212 28-29 0.2666173509960359 2212 30-31 0.30467186234506727 2212 32-33 0.40349423719533917 2212 34-35 0.4039669977497411 2212 36-37 0.5152531935221702 2212 38-39 0.5193042116424422 2212 40-41 0.4268479825380851 2212 42-43 0.3630895044683342 2212 44-45 0.45166650289917953 2212 46-47 0.3035054761313205 2212 48-49 0.36099613495765936 2212 50-51 0.3382011699880536 2212 52-53 0.33962809927746207 2212 54-55 0.2659912956825039 2212 56-57 0.3423044558033723 2212 58-59 0.35562068575438843 2212 60-61 0.4167572722468904 2212 62-63 0.39621855875669354 2212 64-65 0.46085330691732196 2212 66-67 0.4869705338104282 2212 68-69 0.4809809943811487 2212 70-71 0.30867896811057705 2212 72-73 0.4072623509239719 2212 74-75 0.42717735072389473 2212 76-77 0.6377426717573371 2212 78-79 0.5080061443644581 2212 80-81 0.42709222630644206 2212 82-83 0.37964702844437426 2212 84-85 0.4151339623933552 2212 86-87 0.37033458896189586 2212 88-89 0.6483682526158105 2212 90-91 0.4452605191535923 2212 92-93 0.49685409245275025 2212 94-95 0.5582084720608691 2212 96-97 0.3901924953543343 2212 98-99 0.6859324885386755 2212 100 0.5158425622033924 2213 1 -0.498634310512017 2213 2 -25.996767629082285 2213 3 -14.86867703705974 2213 4 -5.952528874191209 2213 5 -1.9039524124470688 2213 6 -1.1002663841439642 2213 7 -0.9199376771913705 2213 8 -0.9192107256150877 2213 9 -0.8721519417264147 2213 10-11 -0.8284315044187878 2213 12-13 -0.8280307033024386 2213 14-15 -1.013739135726503 2213 16-17 -1.149415842374296 2213 18-19 -1.0830204022405638 2213 20-21 -1.0834345979401903 2213 22-23 -1.04239621120076 2213 24-25 -1.1142455926569639 2213 26-27 -1.1673268367701581 2213 28-29 -1.1174508805783105 2213 30-31 -1.1626943125567166 2213 32-33 -1.0771982162451579 2213 34-35 -1.1329141635922682 2213 36-37 -1.3073954819901275 2213 38-39 -1.3076329025038476 2213 40-41 -1.19485860476928 2213 42-43 -1.1616123626244743 2213 44-45 -1.0940740309659773 2213 46-47 -1.0677034643213545 2213 48-49 -1.153519569100844 2213 50-51 -1.019123597267992 2213 52-53 -1.1790424271758866 2213 54-55 -1.2916954403753564 2213 56-57 -1.115403460704421 2213 58-59 -1.1006199959187128 2213 60-61 -1.1488665094086414 2213 62-63 -1.0004693115664693 2213 64-65 -0.9237251566083913 2213 66-67 -1.0536170723863663 2213 68-69 -1.1779326279417077 2213 70-71 -1.0040084694994746 2213 72-73 -1.1665434840623803 2213 74-75 -1.209467344684633 2213 76-77 -1.2452208828737668 2213 78-79 -1.0063830393072664 2213 80-81 -1.1374007710244527 2213 82-83 -1.2276951437326034 2213 84-85 -1.2367764552819054 2213 86-87 -1.046788666797255 2213 88-89 -1.0920032112397884 2213 90-91 -1.0741801081090472 2213 92-93 -1.0466746776137867 2213 94-95 -1.0463763589882262 2213 96-97 -1.135836162249639 2213 98-99 -1.038175817196695 2213 100 -1.2735118384032624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 29.0 5 52.0 6 45.0 7 42.0 8 51.0 9 70.0 10 316.0 11 1355.0 12 3821.0 13 7279.0 14 9571.0 15 11106.0 16 12085.0 17 13008.0 18 13714.0 19 14918.0 20 16772.0 21 19701.0 22 21415.0 23 23532.0 24 24665.0 25 26571.0 26 29064.0 27 33457.0 28 39821.0 29 47816.0 30 58705.0 31 72840.0 32 93630.0 33 126326.0 34 176646.0 35 254324.0 36 391289.0 37 673799.0 38 673431.0 39 4480.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.51629683240553 20.838365151107485 10.732458857285481 29.9128791592015 2 13.007419706927381 22.873547119202993 36.368102807644966 27.750930366224658 3 15.340157796280698 27.5857901158211 28.944984717305548 28.129067370592647 4 10.786896433112837 19.028601543456954 37.755536200371736 32.42896582305847 5 11.890703691405168 38.33440561182135 35.40722369804637 14.367666998727103 6 29.562827179903593 39.641885938390836 17.948860593286227 12.846426288419345 7 25.589102408643782 33.28925213272173 23.568656469952582 17.55298898868191 8 24.337664832355372 36.68813759822226 21.18823018902343 17.785967380398944 9 24.81641043358093 16.77270685783382 21.919606149594056 36.491276558991196 10-11 22.988383040102264 27.741472978692162 29.14067317907392 20.129470802131646 12-13 23.9282382252127 25.325360340215124 29.27836258946427 21.468038845107902 14-15 21.727353006412972 26.57603562132443 26.48140611363971 25.21520525862289 16-17 20.767508003367514 30.189873352128476 27.630661214439122 21.411957430064888 18-19 20.78289431299848 29.239326001637032 29.491426827458955 20.486352857905533 20-21 21.989645080273252 28.546079876368243 28.59395471371987 20.870320329638634 22-23 21.04740483825478 28.631743730594987 28.784488182598363 21.536363248551872 24-25 21.30072533662897 28.43387285271499 28.54360853548112 21.72179327517492 26-27 21.234277397513395 28.432740866919897 28.6628681674158 21.670113568150907 28-29 20.92741873506459 28.30686775292206 29.229316150264467 21.536397361748882 30-31 21.69506161858566 28.71300877107385 28.46380561151837 21.12812399882212 32-33 20.576550710513892 28.731255531869266 29.18886177224947 21.503331985367375 34-35 20.755674500194516 28.21586092110877 29.336627698464408 21.69183688023231 36-37 21.536696277404467 28.632066797265875 28.58575559183631 21.245481333493345 38-39 21.22207582976034 28.59640519207823 28.834873332147616 21.34664564601382 40-41 21.35742174515391 28.382049661973323 28.70825649862349 21.552272094249272 42-43 21.102999239695524 28.5045624312199 28.935706698950682 21.45673163013389 44-45 21.585805180232683 28.00070937490395 28.755689418122632 21.65779602674074 46-47 21.40098954330763 28.62690072618318 28.802704406317186 21.169405324192002 48-49 21.295485668956196 28.49706121762361 28.815179595518675 21.39227351790152 50-51 21.504680873993422 28.41616757262928 28.402212858440134 21.676938694937167 52-53 21.000252685888643 28.67005527136789 28.36116706682462 21.968524975918847 54-55 21.426394028738173 28.567310633386985 28.13402903869241 21.872266299182435 56-57 21.613015811777693 28.32012533509298 28.192348335401928 21.874510517727398 58-59 21.095993128175618 28.000145753412163 28.877584748528733 22.026276369883476 60-61 21.740838552998476 28.40498856713306 28.15315638134608 21.70101649852239 62-63 20.91385505183998 28.67910158425605 28.52750623228214 21.879537131621827 64-65 20.959608781417533 28.096056209693494 28.74230741731283 22.202027591576147 66-67 21.362758870336258 28.381742838915187 28.348100100617344 21.90739819013121 68-69 21.505868681842433 28.283649127146166 28.357589492281342 21.85289269873006 70-71 21.53018177547374 28.197884741102293 28.147745142996307 22.124188340427665 72-73 21.31807379569053 28.41070182589412 28.519405738129617 21.751818640285734 74-75 21.74636258658514 27.813634829634577 28.23652159615445 22.203480987625834 76-77 21.356205244826256 28.43270369259974 28.432047433058074 21.77904362951593 78-79 21.452261140782564 28.268054700008065 28.588779234899636 21.690904924309734 80-81 21.499573023365855 28.10963758361446 28.31816105610447 22.07262833691522 82-83 20.87832844068382 28.44788464859264 28.483923329304094 22.189863581419445 84-85 21.187136690279747 27.999702289367136 28.20984386078885 22.603317159564266 86-87 21.40714152748743 28.232383706091586 28.13581963932147 22.224655127099517 88-89 21.054350200438435 27.905709754094893 28.602284039782045 22.437656005684627 90-91 21.434792879463114 27.955038694365626 28.283771574768124 22.32639685140314 92-93 20.773243575196933 28.31998593744685 28.491830889510016 22.414939597846207 94-95 20.68903535050929 27.805619682876248 28.72447737432724 22.780867592287223 96-97 21.104676360233242 28.05781606396994 28.385366754899877 22.45214082089694 98-99 20.9964501295783 27.813482531836858 28.506535204565193 22.68353213401965 100 21.053444910540257 27.708977364890792 28.463354244264476 22.774223480304475 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6.0 1 6.5 2 10.0 3 10.0 4 15.0 5 25.0 6 47.0 7 87.5 8 138.5 9 181.0 10 170.5 11 154.0 12 192.5 13 240.5 14 296.5 15 364.5 16 416.0 17 485.0 18 625.5 19 809.0 20 1052.5 21 1475.0 22 2108.0 23 3236.5 24 4928.0 25 7340.0 26 11115.5 27 16863.5 28 24981.0 29 34590.0 30 44914.0 31 56179.5 32 67676.5 33 78273.0 34 88200.0 35 96957.0 36 104369.5 37 111405.0 38 116919.5 39 120540.5 40 123132.5 41 123865.0 42 121889.0 43 118815.0 44 114092.5 45 108192.0 46 103193.0 47 98824.0 48 94684.5 49 93611.5 50 105576.0 51 100856.5 52 77979.0 53 67135.5 54 61369.0 55 56427.0 56 51581.5 57 46926.5 58 41708.5 59 36583.5 60 31491.0 61 26138.5 62 21381.5 63 17167.5 64 13401.0 65 10485.5 66 8136.0 67 6257.5 68 4647.5 69 3431.0 70 2556.0 71 1912.5 72 1508.5 73 1118.5 74 774.5 75 547.5 76 392.5 77 254.0 78 155.0 79 81.0 80 34.5 81 18.5 82 12.0 83 5.0 84 2.0 85 0.0 86 1.0 87 2.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6583766121421016 2 4.82964915243292 3 0.0 4 0.0062505201572368415 5 0.16296245647514176 6 0.05901734225810919 7 0.03802111985147935 8 0.04499683847999782 9 0.043477375016360134 10-11 0.044340706529790636 12-13 0.0427349099148099 14-15 0.04534217108537002 16-17 0.03591459095870893 18-19 0.03057920220570842 20-21 0.02451861498142629 22-23 0.025122947040827636 24-25 0.021427888163345087 26-27 0.013985970517574149 28-29 0.02015015752346794 30-31 0.02526108008297652 32-33 0.0013122639004143647 34-35 0.0037123255077511626 36-37 0.004541123760644446 38-39 0.006699452544220704 40-41 0.0070275185193242945 42-43 0.007200184822010396 44-45 0.008702381655379472 46-47 0.01671409810001454 48-49 0.026504277462316445 50-51 0.02348261716530968 52-53 0.029715870692277917 54-55 0.02622801137801868 56-57 0.01659323168813427 58-59 0.015816233326046814 60-61 0.013070839113337816 62-63 0.019045093186276896 64-65 0.020719956322332072 66-67 0.021825020659523117 68-69 0.029646804171203474 70-71 0.02905973874207073 72-73 0.025520079537005667 74-75 0.026797810176882816 76-77 0.01944222568245493 78-79 0.015177368006108244 80-81 0.01386510410569388 82-83 0.008944114479140012 84-85 0.01139597597728264 86-87 0.009393046866123873 88-89 0.007994449814366457 90-91 0.00852971535269337 92-93 0.004333924197421125 94-95 0.0015194634636376853 96-97 8.287982528932829E-4 98-99 6.733985804757923E-4 100 0.10957403568459943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2895759.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.95372444127005 #Duplication Level Percentage of deduplicated Percentage of total 1 87.17489939769973 70.57132784036823 2 9.471238120499512 15.334640018491399 3 2.0402314471130247 4.954930030980043 4 0.6219460718334233 2.013954036661332 5 0.2579795095745816 1.0442201064797332 6 0.12361608479197217 0.6004309478854792 7 0.07567496285143206 0.4288319062844725 8 0.05188495933417088 0.33602245604679776 9 0.03489119617792041 0.2542115052732297 >10 0.1372897544195354 1.8580211038226633 >50 0.005877161876609505 0.3173962878021512 >100 0.0030592471712029687 0.42727341914562567 >500 8.558100952425271E-4 0.5099082675812089 >1k 4.7069555219384125E-4 0.7705780848486948 >5k 8.558100948978932E-5 0.5782539883290295 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9433 0.3257522466475974 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7260 0.25071147150021805 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3154 0.10891790373439227 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.4533260537220124E-5 0.0 12-13 0.0 0.0 0.0 1.2086641188027041E-4 0.0 14-15 0.0 0.0 0.0 2.589994540291509E-4 0.0 16-17 0.0 0.0 0.0 2.76266084297761E-4 0.0 18-19 0.0 0.0 0.0 3.107993448349811E-4 0.0 20-21 0.0 0.0 0.0 4.316657567152515E-4 0.0 22-23 0.0 0.0 0.0 5.007322777896918E-4 0.0 24-25 0.0 0.0 0.0 6.561319502071824E-4 0.0 26-27 0.0 0.0 0.0 7.597317318188426E-4 0.0 28-29 0.0 0.0 0.0 0.001174130858265484 0.0 30-31 0.0 0.0 0.0 0.0032633931207673014 0.0 32-33 0.0 0.0 0.0 0.009962845664988005 0.0 34-35 0.0 0.0 0.0 0.019286826010037436 0.0 36-37 0.0 0.0 0.0 0.03399799499889321 0.0 38-39 0.0 0.0 0.0 0.056997146516681814 0.0 40-41 0.0 0.0 0.0 0.08519355374532203 0.0 42-43 0.0 0.0 0.0 0.11409789281497527 0.0 44-45 0.0 0.0 0.0 0.14818222096521155 0.0 46-47 0.0 1.7266630268610062E-5 0.0 0.18901780155047432 0.0 48-49 0.0 3.4533260537220124E-5 6.906652107444025E-5 0.23242611004576003 0.0 50-51 0.0 3.4533260537220124E-5 6.906652107444025E-5 0.2803237424108843 0.0 52-53 0.0 3.4533260537220124E-5 6.906652107444025E-5 0.334627294605663 0.0 54-55 0.0 3.4533260537220124E-5 6.906652107444025E-5 0.3952677001090215 0.0 56-57 0.0 5.1799890805830186E-5 6.906652107444025E-5 0.465076686285012 0.0 58-59 0.0 6.906652107444025E-5 6.906652107444025E-5 0.5437261871585308 0.0 60-61 0.0 6.906652107444025E-5 6.906652107444025E-5 0.6274693439612896 0.0 62-63 0.0 6.906652107444025E-5 6.906652107444025E-5 0.7141650945399807 0.0 64-65 0.0 6.906652107444025E-5 6.906652107444025E-5 0.814225216946576 0.0 66-67 0.0 6.906652107444025E-5 6.906652107444025E-5 0.9305159718056648 0.0 68-69 0.0 6.906652107444025E-5 6.906652107444025E-5 1.0620358945616677 0.0 70-71 0.0 8.63331513430503E-5 6.906652107444025E-5 1.2096137834674776 0.0 72-73 0.0 1.0359978161166036E-4 6.906652107444025E-5 1.3644263904558356 0.0 74-75 0.0 1.0359978161166036E-4 6.906652107444025E-5 1.5272161806282911 0.0 76-77 0.0 1.0359978161166036E-4 6.906652107444025E-5 1.713246855142296 0.0 78-79 0.0 1.0359978161166036E-4 6.906652107444025E-5 1.9167686261184027 0.0 80-81 0.0 1.0359978161166036E-4 6.906652107444025E-5 2.139715356146696 0.0 82-83 0.0 1.0359978161166036E-4 6.906652107444025E-5 2.389425363091335 0.0 84-85 0.0 1.0359978161166036E-4 6.906652107444025E-5 2.647423352564906 0.0 86-87 0.0 1.0359978161166036E-4 6.906652107444025E-5 2.9269700966137027 0.0 88 0.0 1.0359978161166036E-4 6.906652107444025E-5 3.1589300076422107 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTCG 125 0.006446271 18.783394 9 CATGGGG 4220 0.0 16.600779 4 CTATGCG 170 0.0019309592 16.573584 9 GAGTACT 3310 0.0 16.386099 12-13 GTATATA 2050 0.0 16.161295 1 CTAGGAC 1145 0.0 15.603075 3 GTATTAG 1215 0.0 15.465446 1 GTATAAG 1070 0.0 14.788406 1 GTCTATA 1065 0.0 13.929221 1 GTACATG 17250 0.0 13.759648 1 GTATAGG 900 0.0 13.73576 1 TAGTACG 310 3.8528902E-5 13.650088 4 TAGGACA 1435 0.0 13.43342 4 ACATGGG 16495 0.0 13.424579 3 CATGGGT 2105 0.0 13.401511 4 TACATGG 17580 0.0 13.375223 2 AGTACTT 4090 0.0 13.261121 12-13 AACCGTC 290 3.3875796E-4 12.954065 7 GTATAGC 690 2.0008883E-11 12.89967 1 TATACTG 1605 0.0 12.889387 5 >>END_MODULE