FastQCFastQC Report
Wed 25 May 2016
SRR1295106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422794
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27190.11222580211111635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4553.6379788E-1220.1074961
ACATCGG4151.6570993E-917.3368133
TACATCG3751.109307E-616.263292
GTATAAG9500.015.4086931
GTATATA15750.015.1029641
GTATTAA14600.015.0393061
GTCTAGA8600.014.8935761
GTCCTAG7551.8189894E-1214.5413161
GTACATG106550.014.4825541
GTCTAAT8450.014.43615151
GTATAAT11000.014.4164661
GTACATC7251.8189894E-1114.3017451
TACATGG108150.014.0978592
GTATTGC6507.930794E-1014.0752491
GTATAGG7001.6189006E-1013.9411981
GTATATG13250.013.8096771
CCGTGCA4051.2429664E-613.6271769
GTATTAG9000.013.5539421
GTGTATA12300.013.388651
GTGTAAT11100.013.1876181
Produced by FastQC (version 0.11.5)