##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295106_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2422794 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.316825945581837 27.0 27.0 33.0 15.0 33.0 2 22.74222612405347 27.0 15.0 33.0 2.0 33.0 3 24.933454928483396 27.0 15.0 33.0 15.0 33.0 4 25.409594459949957 33.0 15.0 37.0 2.0 37.0 5 27.056181416992118 33.0 15.0 37.0 2.0 37.0 6 27.71062459292866 33.0 15.0 37.0 6.0 37.0 7 27.30380956862201 33.0 15.0 37.0 6.0 37.0 8 28.11576180228282 33.0 22.0 37.0 6.0 37.0 9 29.227586001946513 33.0 27.0 37.0 15.0 37.0 10-11 28.75088926256215 33.0 24.5 37.0 10.5 37.0 12-13 27.541195206856216 33.0 15.0 37.0 4.0 37.0 14-15 29.22597711567719 33.0 22.0 37.0 10.5 40.0 16-17 29.691672300657835 33.0 27.0 37.0 6.0 40.0 18-19 29.829714577467172 33.0 27.0 37.0 10.5 40.0 20-21 29.356006949001852 33.0 24.5 37.0 6.0 40.0 22-23 30.096495822591603 33.0 27.0 37.0 15.0 40.0 24-25 30.17881070367518 33.0 27.0 37.0 15.0 40.0 26-27 28.883834325163427 33.0 22.0 37.0 6.0 40.0 28-29 29.52601768866854 33.0 24.5 37.0 10.5 40.0 30-31 30.205781630629758 33.0 27.0 37.0 15.0 40.0 32-33 29.012273639442725 33.0 22.0 37.0 6.0 40.0 34-35 28.567298540445453 33.0 22.0 37.0 6.0 40.0 36-37 28.834132617135424 33.0 22.0 37.0 6.0 40.0 38-39 28.95652209804053 33.0 24.5 37.0 6.0 40.0 40-41 28.11475263683169 33.0 22.0 37.0 6.0 40.0 42-43 28.688063657083518 33.0 22.0 37.0 6.0 40.0 44-45 28.510570853320587 33.0 22.0 37.0 6.0 40.0 46-47 28.2346852435659 33.0 22.0 37.0 6.0 40.0 48-49 28.02535997695223 33.0 22.0 37.0 6.0 40.0 50-51 27.997896643296954 33.0 22.0 37.0 6.0 40.0 52-53 28.195422516317937 33.0 22.0 37.0 6.0 40.0 54-55 28.163242933571738 33.0 22.0 37.0 6.0 40.0 56-57 28.160813507050122 33.0 22.0 37.0 6.0 40.0 58-59 27.950465454347338 33.0 22.0 37.0 6.0 40.0 60-61 27.921028160049925 33.0 22.0 37.0 6.0 40.0 62-63 27.805774036092217 33.0 22.0 37.0 6.0 40.0 64-65 27.423749811168427 33.0 22.0 37.0 6.0 40.0 66-67 27.64763492067423 33.0 22.0 37.0 6.0 37.0 68-69 27.234550688172416 33.0 22.0 37.0 6.0 37.0 70-71 27.320118218882826 33.0 22.0 37.0 6.0 37.0 72-73 27.32223498985056 33.0 22.0 37.0 10.5 37.0 74-75 27.420828803439335 30.0 22.0 37.0 10.5 37.0 76-77 27.175401210338144 27.0 22.0 35.0 10.5 37.0 78-79 26.766120231435277 27.0 22.0 33.0 6.0 37.0 80-81 26.569019487418245 27.0 22.0 33.0 6.0 37.0 82-83 26.641731612345087 27.0 22.0 33.0 10.5 37.0 84-85 26.585610456357415 27.0 22.0 33.0 10.5 37.0 86-87 26.349094062474975 27.0 22.0 33.0 10.5 37.0 88-89 26.276715230432302 27.0 22.0 33.0 6.0 37.0 90-91 26.39850911798527 27.0 22.0 33.0 15.0 37.0 92-93 26.330692993296168 27.0 22.0 33.0 10.5 37.0 94-95 26.2217918650946 27.0 22.0 33.0 10.5 37.0 96-97 26.301694655014003 27.0 22.0 33.0 10.5 37.0 98-99 26.36863967799161 27.0 22.0 33.0 15.0 37.0 100 26.02886378288868 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.400565677377557 1101 2 11.242910069287227 1101 3 8.343164636702213 1101 4 12.983340714695164 1101 5 10.76223876366431 1101 6 10.087079098793474 1101 7 9.691674003029242 1101 8 9.200986208820023 1101 9 7.233434598564084 1101 10-11 7.794458666221363 1101 12-13 9.187977390544223 1101 14-15 8.01276262086795 1101 16-17 8.079232882371073 1101 18-19 8.248634482965226 1101 20-21 7.1081504878137345 1101 22-23 7.360673316742226 1101 24-25 7.429791538025462 1101 26-27 8.410675685184994 1101 28-29 8.073342842085886 1101 30-31 5.568284174028779 1101 32-33 6.966842086875076 1101 34-35 8.770949268232695 1101 36-37 8.248775008181905 1101 38-39 8.206257308701002 1101 40-41 8.950888050478834 1101 42-43 8.504188946503248 1101 44-45 8.451821583065435 1101 46-47 7.295984062765513 1101 48-49 5.3187006635802945 1101 50-51 6.708298909885556 1101 52-53 5.843483227032678 1101 54-55 4.021114191894551 1101 56-57 6.193543250401699 1101 58-59 6.186388791677338 1101 60-61 6.697186219762056 1101 62-63 6.255798873766793 1101 64-65 6.802043486849218 1101 66-67 5.782747774620503 1101 68-69 6.715810624926849 1101 70-71 6.330386269621837 1101 72-73 5.439317415331079 1101 74-75 4.897469904246947 1101 76-77 5.074217627969375 1101 78-79 5.122429679597754 1101 80-81 4.9840487764561665 1101 82-83 4.332711076831345 1101 84-85 4.176762536148708 1101 86-87 3.9418002153957303 1101 88-89 4.002629635397309 1101 90-91 3.9004124885438465 1101 92-93 3.6902998835865297 1101 94-95 3.6231979348001246 1101 96-97 3.8527015757295864 1101 98-99 3.5082601933105266 1101 100 3.751456408402273 1102 1 6.80960577386508 1102 2 10.369606334046445 1102 3 8.062276813406612 1102 4 12.620582038385056 1102 5 10.674699666834275 1102 6 9.645738648376756 1102 7 6.613448116281276 1102 8 7.758135319748813 1102 9 6.405348205854015 1102 10-11 7.464712367476441 1102 12-13 9.085096339164753 1102 14-15 6.050561273520824 1102 16-17 7.170552008752736 1102 18-19 7.443332332174922 1102 20-21 7.025821242721083 1102 22-23 6.484914944444821 1102 24-25 7.060253826510952 1102 26-27 8.475946814067793 1102 28-29 7.687112841873478 1102 30-31 4.189499166103207 1102 32-33 4.245510567661206 1102 34-35 4.845249176099582 1102 36-37 5.023582643812723 1102 38-39 4.551463001836691 1102 40-41 5.938004682496018 1102 42-43 6.73521853729418 1102 44-45 6.23993498511361 1102 46-47 5.46101163300041 1102 48-49 3.6236866710305975 1102 50-51 4.8040531202623455 1102 52-53 5.718429029135013 1102 54-55 5.34511519818987 1102 56-57 6.27959688341987 1102 58-59 6.077814206342698 1102 60-61 6.012367406022282 1102 62-63 4.39410220078091 1102 64-65 6.21538979382505 1102 66-67 3.636695226887852 1102 68-69 6.0499865812392954 1102 70-71 5.224753886693275 1102 72-73 5.037462008551461 1102 74-75 4.158771012233849 1102 76-77 3.329468119832722 1102 78-79 4.420522398161648 1102 80-81 4.396218179237152 1102 82-83 3.7994203749735505 1102 84-85 3.016113313040556 1102 86-87 3.29495638831391 1102 88-89 3.4330722427111375 1102 90-91 3.419420501070764 1102 92-93 3.2724131502229277 1102 94-95 3.143026715244538 1102 96-97 3.417835649977718 1102 98-99 3.1359090035455317 1102 100 3.3213624698048747 1103 1 6.728303175392064 1103 2 10.880890062139454 1103 3 7.832980548440446 1103 4 11.940547225787451 1103 5 10.296371864474391 1103 6 9.12771038618692 1103 7 7.992039995510844 1103 8 8.149280339703697 1103 9 7.171344106426634 1103 10-11 7.530603071103027 1103 12-13 8.918825245550313 1103 14-15 8.63742765170927 1103 16-17 8.167420772851294 1103 18-19 7.353208644425489 1103 20-21 8.400844288443796 1103 22-23 6.9611615824805675 1103 24-25 5.212371321739134 1103 26-27 5.940356897791553 1103 28-29 7.080106671042309 1103 30-31 6.1415407460095075 1103 32-33 7.973837556642643 1103 34-35 7.140077173670296 1103 36-37 4.05846925885697 1103 38-39 6.296902660000843 1103 40-41 6.642369542378024 1103 42-43 7.507177857526699 1103 44-45 5.604853430367818 1103 46-47 6.430015407076411 1103 48-49 5.632348609257214 1103 50-51 6.404162384100623 1103 52-53 6.064651970745288 1103 54-55 4.8650204667132115 1103 56-57 5.7193645825349115 1103 58-59 5.493862434262187 1103 60-61 5.077373153896648 1103 62-63 2.7078729690439687 1103 64-65 5.10772993784726 1103 66-67 4.075871599870673 1103 68-69 5.100244693333689 1103 70-71 3.6689032555116015 1103 72-73 4.435343699944834 1103 74-75 3.1562950882359324 1103 76-77 3.472813057365258 1103 78-79 2.919298805848616 1103 80-81 4.101433832976522 1103 82-83 3.3245331634517683 1103 84-85 3.130475407435835 1103 86-87 2.678429080488126 1103 88-89 3.0327940672816744 1103 90-91 2.6668442603975038 1103 92-93 2.185833352374587 1103 94-95 2.6938891675263363 1103 96-97 2.9189152743053235 1103 98-99 2.6752003340157735 1103 100 2.794150622676643 1104 1 6.422597457543727 1104 2 9.72480391010955 1104 3 7.315766973077984 1104 4 12.082063108085098 1104 5 9.433169782316096 1104 6 8.018127059765547 1104 7 3.9552571802979024 1104 8 6.400889650940886 1104 9 6.5580571454211345 1104 10-11 7.009983213599956 1104 12-13 7.359813728154297 1104 14-15 7.143129481581095 1104 16-17 7.115978333565934 1104 18-19 6.741833582909063 1104 20-21 7.394638645935803 1104 22-23 6.390735121569069 1104 24-25 5.921787793488569 1104 26-27 8.123677357602663 1104 28-29 7.053804475389654 1104 30-31 6.276631963122409 1104 32-33 7.413735608714568 1104 34-35 7.8582714522277435 1104 36-37 6.406129474593012 1104 38-39 7.344902408405563 1104 40-41 7.1259546265103815 1104 42-43 7.231715015523008 1104 44-45 6.687241622531314 1104 46-47 6.623548578946398 1104 48-49 5.2730133875768885 1104 50-51 5.612559048251335 1104 52-53 5.878632494920552 1104 54-55 4.67169926732257 1104 56-57 5.904834125682122 1104 58-59 3.6553752165465596 1104 60-61 4.161522754938421 1104 62-63 3.8270329345671215 1104 64-65 2.6909233731458038 1104 66-67 3.0188506573284 1104 68-69 3.99317452036885 1104 70-71 2.412146729931827 1104 72-73 2.160540569795426 1104 74-75 3.0967556873091766 1104 76-77 3.711228926391943 1104 78-79 4.348873779168457 1104 80-81 3.1463629266529125 1104 82-83 2.983807421660412 1104 84-85 2.927020810707127 1104 86-87 0.49433477511227863 1104 88-89 0.10445541865245289 1104 90-91 2.1999249270973493 1104 92-93 2.3938690222262835 1104 94-95 2.4258614498825963 1104 96-97 2.869363411117863 1104 98-99 2.549271384108117 1104 100 0.8945718028520382 1105 1 5.6954536363227035 1105 2 -2.6545604426893448 1105 3 0.509382545806556 1105 4 8.316954710423097 1105 5 8.342500595817679 1105 6 7.845607115774278 1105 7 5.16509750209557 1105 8 5.734382402917046 1105 9 4.6159693259174155 1105 10-11 4.7056113020073305 1105 12-13 4.832681972942762 1105 14-15 5.6134880665468465 1105 16-17 5.812741503755596 1105 18-19 5.943909094381592 1105 20-21 6.760615743667675 1105 22-23 1.892353966150452 1105 24-25 3.80510892050998 1105 26-27 6.634703638353447 1105 28-29 5.66835431151641 1105 30-31 5.065259468573164 1105 32-33 6.97388831388583 1105 34-35 5.812234768051329 1105 36-37 5.967494445424133 1105 38-39 3.8790251358568533 1105 40-41 5.0457510722287715 1105 42-43 5.2179918939621714 1105 44-45 5.9189227894547365 1105 46-47 5.112406759139624 1105 48-49 4.626861339722534 1105 50-51 5.733180437217392 1105 52-53 5.41967693782167 1105 54-55 5.101059481199602 1105 56-57 4.873095925818497 1105 58-59 1.5580922959829948 1105 60-61 4.113482131069606 1105 62-63 -1.1120828584362812 1105 64-65 -0.5009948207128794 1105 66-67 0.5450948768680419 1105 68-69 2.48392006646802 1105 70-71 2.5774236132815744 1105 72-73 2.331789321103745 1105 74-75 2.314531479807485 1105 76-77 1.4787804629842185 1105 78-79 2.924930116208582 1105 80-81 1.092301644653432 1105 82-83 0.4432721889127009 1105 84-85 1.080848541764194 1105 86-87 -0.6766603620052898 1105 88-89 1.2465149847531407 1105 90-91 1.1216703139532527 1105 92-93 1.4168117325326186 1105 94-95 0.7147119835930624 1105 96-97 1.740504936289522 1105 98-99 1.710633005218586 1105 100 1.8626180282956035 1106 1 5.9718734688509905 1106 2 10.212466196232509 1106 3 6.668899281835259 1106 4 11.65655277274308 1106 5 9.816109873059684 1106 6 9.313301956241595 1106 7 6.488965703083906 1106 8 7.506750615047881 1106 9 7.004578700656264 1106 10-11 5.598642959230727 1106 12-13 7.892638489352873 1106 14-15 7.789310522018649 1106 16-17 7.622052556275747 1106 18-19 7.11578484301803 1106 20-21 8.071380752609532 1106 22-23 5.902356236433977 1106 24-25 4.72377956504765 1106 26-27 7.5144080349037345 1106 28-29 6.051771944794588 1106 30-31 5.205059906473242 1106 32-33 7.124470509291783 1106 34-35 6.581682497210682 1106 36-37 5.836214569607254 1106 38-39 7.044713108368317 1106 40-41 6.420295657532936 1106 42-43 5.9864051468466855 1106 44-45 4.294965796702929 1106 46-47 5.840162959938539 1106 48-49 5.680074522130763 1106 50-51 6.388830204896355 1106 52-53 5.4414263584118885 1106 54-55 5.255671986979774 1106 56-57 4.6876409916427875 1106 58-59 4.766749492921036 1106 60-61 4.7082233257122965 1106 62-63 4.895172368787065 1106 64-65 5.0797421021754765 1106 66-67 2.8238816192479703 1106 68-69 3.1389694973650464 1106 70-71 2.7269073327023854 1106 72-73 2.556423812420956 1106 74-75 2.6347060130761903 1106 76-77 3.3755374743997066 1106 78-79 4.116844703406965 1106 80-81 3.6483455084809897 1106 82-83 1.8027857214738603 1106 84-85 2.5574350938860064 1106 86-87 3.095126281484987 1106 88-89 3.156953309646031 1106 90-91 3.089889582949816 1106 92-93 2.764313236769553 1106 94-95 1.3110971838515653 1106 96-97 1.342567029240218 1106 98-99 1.716545909900102 1106 100 2.645272296150061 1107 1 5.969023745595244 1107 2 10.317067094497386 1107 3 5.994644725288321 1107 4 11.334821024016424 1107 5 9.231324585659078 1107 6 7.7014483116061 1107 7 3.5683104272828743 1107 8 6.176240915954573 1107 9 5.479860361683688 1107 10-11 4.34817436037142 1107 12-13 5.872775717575568 1107 14-15 5.897806711015502 1107 16-17 6.862263154838047 1107 18-19 6.147899417947681 1107 20-21 5.377400893329668 1107 22-23 5.617824413855335 1107 24-25 4.40221338122846 1107 26-27 6.9986833823893555 1107 28-29 6.133054280122728 1107 30-31 4.858646945450236 1107 32-33 4.650066843985758 1107 34-35 6.423285045104098 1107 36-37 6.330543218958837 1107 38-39 6.352578545238924 1107 40-41 7.434242243283514 1107 42-43 5.972173588770527 1107 44-45 3.7527718308915787 1107 46-47 3.272116786394406 1107 48-49 3.896796979154125 1107 50-51 4.624551485907162 1107 52-53 3.649347786323922 1107 54-55 5.144911070733539 1107 56-57 5.1046197230744355 1107 58-59 2.781977450713022 1107 60-61 2.4319386423727423 1107 62-63 0.07665859980730971 1107 64-65 1.7094576917671667 1107 66-67 3.7820428654199034 1107 68-69 4.5986188208567995 1107 70-71 3.9701412468173487 1107 72-73 2.044947655774479 1107 74-75 2.122418086671299 1107 76-77 2.6954018516549034 1107 78-79 3.6875148298784914 1107 80-81 1.7714374983504264 1107 82-83 1.32736358113101 1107 84-85 2.052126982610332 1107 86-87 1.9987062964962021 1107 88-89 2.3073604989760277 1107 90-91 1.1474590098343427 1107 92-93 2.0443697897282505 1107 94-95 2.0319146129195502 1107 96-97 2.0413041201945568 1107 98-99 1.639203015945931 1107 100 -0.14037834383356085 1108 1 4.228641222008999 1108 2 -2.9200417707089716 1108 3 0.13206457541308225 1108 4 6.492652411948939 1108 5 6.40936809913412 1108 6 6.381646424622378 1108 7 3.933678315026082 1108 8 4.664627861196493 1108 9 4.563250607312597 1108 10-11 3.267479547707058 1108 12-13 2.2213537590475667 1108 14-15 3.579830489796901 1108 16-17 4.300544111671762 1108 18-19 3.513880765867963 1108 20-21 4.308921756833531 1108 22-23 3.692590210551355 1108 24-25 2.523008544739149 1108 26-27 4.2921775300515215 1108 28-29 5.799506205912039 1108 30-31 4.336836873478717 1108 32-33 4.1079763341785664 1108 34-35 2.7263648544175645 1108 36-37 0.7220645777487533 1108 38-39 1.9298236835608797 1108 40-41 4.925113475996472 1108 42-43 2.531226575889093 1108 44-45 3.286894917472143 1108 46-47 4.391346975776777 1108 48-49 4.7100621426065885 1108 50-51 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2213 40-41 -16.982054543936126 2213 42-43 -23.11111258364977 2213 44-45 -16.89764977153503 2213 46-47 -16.79412236622887 2213 48-49 -5.826536861727448 2213 50-51 -23.252547174722906 2213 52-53 -17.711328754943125 2213 54-55 -17.483111518390317 2213 56-57 -17.393446459455756 2213 58-59 -17.85716660585308 2213 60-61 -19.195715900932125 2213 62-63 -17.345752090038765 2213 64-65 -17.514107039556453 2213 66-67 -8.607765771290854 2213 68-69 -22.64350530666631 2213 70-71 -17.2840952407376 2213 72-73 -9.389881243259506 2213 74-75 -4.081824899261399 2213 76-77 -2.844346950105848 2213 78-79 -4.751444426319832 2213 80-81 -3.4194791191016627 2213 82-83 -3.0493654200873976 2213 84-85 -2.937655748603561 2213 86-87 -3.2382571215976874 2213 88-89 -2.5734598398021546 2213 90-91 -2.5373884455153473 2213 92-93 -3.60516653708353 2213 94-95 -3.542582173537305 2213 96-97 -3.302556438407752 2213 98-99 -3.671122099506622 2213 100 -3.080192510766473 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 669.0 3 555.0 4 2535.0 5 9529.0 6 16211.0 7 18210.0 8 16324.0 9 16843.0 10 20310.0 11 25995.0 12 34053.0 13 44423.0 14 38615.0 15 43268.0 16 49193.0 17 51739.0 18 52262.0 19 52721.0 20 53601.0 21 53074.0 22 55905.0 23 60848.0 24 66134.0 25 72556.0 26 77866.0 27 84018.0 28 89091.0 29 93912.0 30 99693.0 31 108716.0 32 123076.0 33 145267.0 34 169530.0 35 188336.0 36 192930.0 37 152390.0 38 42315.0 39 81.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.40654665411325 21.364405399857002 14.31850519952146 23.910542746508288 2 14.442839487390932 21.458287751537597 41.12666910008499 22.97220366098648 3 17.385028398386805 27.03816861803821 30.363248161431223 25.213554822143763 4 11.874548216270265 18.020346057049206 40.50933076035533 29.595774966325195 5 12.876280345038902 37.86189130641295 36.57931107704515 12.682517271502997 6 29.66816482850607 38.602546423601424 18.894855046746443 12.834433701146061 7 25.437887218078554 32.056786796002505 24.52662103441988 17.978704951499058 8 23.356304553660927 36.37116855683708 22.89762698436526 17.374899905136733 9 24.247827970326426 17.284851699078498 22.737372816903413 35.729947513691656 10-11 22.665947041662466 27.663628469648017 29.812618523402417 19.8578059652871 12-13 23.798731991722434 25.31292103435936 29.48039670843494 21.407950265483265 14-15 21.227062406183872 26.95628126973803 26.9400207294235 24.876635594654598 16-17 20.584965959579176 30.445873945186168 27.942404020565114 21.02675607466954 18-19 20.68416439220248 29.57663668627461 29.315692847512025 20.423506074010884 20-21 22.056858959357204 28.9694939684701 28.257748618882484 20.715898453290215 22-23 21.013357405115222 29.007851723850187 28.649121907291768 21.32966896374282 24-25 21.293165873212022 28.981466532719345 28.17421173574173 21.5511558583269 26-27 21.41450062843127 28.747695563974755 28.26654511730341 21.571258690290566 28-29 21.18974129327996 28.71603922189443 28.78874281601027 21.30547666881534 30-31 21.756681472752334 29.109288267336847 28.124276056919427 21.00975420299139 32-33 20.929527829097164 28.937976089651396 28.657396152883635 21.475099928367804 34-35 20.94069249892174 28.633338560575783 28.61609525868582 21.80987368181665 36-37 21.65892908331878 29.035432530359994 28.09352316497362 21.212115221347602 38-39 21.533874267949756 28.77935489935152 28.240233455381595 21.446537377317128 40-41 21.422528886474762 28.77022519385842 28.035857342224563 21.77138857744226 42-43 21.330736528106705 28.749307083520552 28.53028641131172 21.38966997706102 44-45 21.840199821796862 28.42165214021881 28.297626777414575 21.44052126056975 46-47 21.58428525721589 28.848123263235443 28.51506386170915 21.052527617839516 48-49 21.5197329710138 28.561004526215694 28.54031520859342 21.378947294177088 50-51 21.651137421925462 28.58129165646568 28.310254079645947 21.45731684196291 52-53 21.16958065332333 28.660674947254655 28.286591297678175 21.883153101743844 54-55 21.67120785562793 28.819855149190726 27.93233394828975 21.576603046891595 56-57 21.715719170765283 28.510187927078878 28.047379062406474 21.726713839749365 58-59 21.505778855211734 28.110559390455858 28.47619719627941 21.907464558052997 60-61 21.934923348200925 28.573348864467736 28.0003921311773 21.49133565615404 62-63 21.356097751640885 28.653843784479392 28.38499745726095 21.60506100661877 64-65 21.326474508282132 28.292459738031546 28.431936472262542 21.949129281423783 66-67 21.57802181118899 28.383264593507253 28.13731939981276 21.901394195491005 68-69 22.168854782138304 28.25518511722719 27.986460461448253 21.58949963918625 70-71 21.905654815000386 28.38455306257668 27.91946552152435 21.790326600898585 72-73 21.77215952422687 28.43109242326439 28.230204113734075 21.566543938774664 74-75 21.99172029010761 28.132176286832777 27.964156287260618 21.911947135798997 76-77 21.80629365893807 28.490990826739456 28.112808705481555 21.58990680884092 78-79 21.947190746100464 28.26994616227448 28.225453039445615 21.557410052179442 80-81 21.915206091344963 28.172344386222196 28.095740344655155 21.816709177777685 82-83 21.39020753643413 28.40196530399498 28.33308414009644 21.87474301947445 84-85 21.609468635801093 28.14916719032824 28.001269305587194 22.240094868283467 86-87 21.90924047082531 28.23114455328064 27.955056077364432 21.904558898529615 88-89 21.704533419719784 27.865922414169038 28.392381379566693 22.037162786544478 90-91 21.97170278549347 27.907047977941062 28.159397886867023 21.961851349698446 92-93 21.567010821314824 28.184175508523158 28.31742937379582 21.9313842963662 94-95 21.386530341181277 27.881232832531737 28.357652099726977 22.374584726560006 96-97 21.674370571291703 27.950906530795915 28.285979723074178 22.088743174838196 98-99 21.70177981440366 27.760256026390028 28.265947516247707 22.2720166429586 100 21.669976332713357 27.772707925145788 28.074718402350495 22.482597339790356 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 279.0 1 328.5 2 295.0 3 208.5 4 246.5 5 357.5 6 479.0 7 665.5 8 1011.5 9 1497.0 10 2086.5 11 2762.5 12 3539.5 13 4316.5 14 5007.5 15 5616.0 16 6216.0 17 6883.0 18 7530.5 19 8253.0 20 8880.0 21 9600.0 22 10682.5 23 12022.0 24 13773.5 25 16182.5 26 19711.5 27 24621.0 28 30837.5 29 37936.0 30 45592.5 31 53781.0 32 61981.5 33 69816.5 34 77033.0 35 83300.0 36 88573.5 37 92607.5 38 95239.0 39 96529.5 40 96915.5 41 95739.0 42 92735.0 43 89210.5 44 85658.0 45 81729.0 46 77930.0 47 74839.0 48 71961.5 49 70512.5 50 70709.5 51 67019.0 52 59078.0 53 51681.5 54 45857.0 55 41395.5 56 37454.0 57 33547.5 58 29885.0 59 26326.0 60 22544.0 61 18991.0 62 15613.5 63 12599.5 64 10269.0 65 8301.5 66 6600.0 67 5114.0 68 4024.0 69 3180.5 70 2422.0 71 1781.5 72 1341.0 73 1038.5 74 788.0 75 572.0 76 369.5 77 263.5 78 189.0 79 134.5 80 98.5 81 63.0 82 45.0 83 33.0 84 24.0 85 21.0 86 15.0 87 7.5 88 5.5 89 5.0 90 5.5 91 4.5 92 2.5 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 6.710681964706863 2 23.22050492117778 3 5.353571124907854 4 22.230367088576248 5 11.363987198251275 6 8.588431373034604 7 7.197640410204087 8 7.890105390718319 9 4.430133143800092 10-11 6.0512160753246045 12-13 9.228890281220773 14-15 6.716274681215159 16-17 6.966440398977379 18-19 6.036439746837742 20-21 6.878422185295159 22-23 5.031298575116168 24-25 4.640633912747019 26-27 8.542162478526857 28-29 5.617976600569425 30-31 3.6112438779359697 32-33 7.721147567642977 34-35 6.16839483670506 36-37 6.049482539580335 38-39 5.150912541470715 40-41 7.029590629661457 42-43 5.661438818157879 44-45 5.570242455611166 46-47 4.924108281595546 48-49 3.4432353720539175 50-51 5.842861588727724 52-53 3.8372020072692936 54-55 4.300881544200622 56-57 4.083735554900665 58-59 3.190675723978184 60-61 3.5843327992392253 62-63 2.849313643669251 64-65 4.061096403573726 66-67 2.1270483582178263 68-69 4.510226622651368 70-71 2.1353445649939697 72-73 1.3377530239880073 74-75 0.6332564799153374 76-77 0.9557766776704911 78-79 1.4357184308694837 80-81 1.7758009967005037 82-83 0.620110500521299 84-85 1.0817263044237355 86-87 0.37456754474379583 88-89 0.6194913806126315 90-91 0.28489834463846286 92-93 0.27773719102820954 94-95 0.43453962656338097 96-97 0.4820467608884618 98-99 0.4029025992304753 100 0.31558605477807855 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2422794.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.67214740395724 #Duplication Level Percentage of deduplicated Percentage of total 1 97.33753999377281 93.12491474222814 2 2.1847739586486865 4.180440324323286 3 0.29079642440102266 0.8346335513951505 4 0.08681827255353533 0.3322436227639507 5 0.03331560616852728 0.15936877921037645 6 0.020105892265336683 0.1154144333098425 7 0.010218339341071124 0.06843273273638283 8 0.007478694193847957 0.057240218664235266 9 0.0035270200326269034 0.030369382241237204 >10 0.022886245763627546 0.3695401908960629 >50 8.900210576556174E-4 0.05391399264621405 >100 0.0013026049493991712 0.2810431421319948 >500 2.60195138931383E-4 0.18997834228501584 >1k 8.673171297351228E-5 0.2024665451680697 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2719 0.11222580211111635 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.1274660577828736E-5 0.0 0.0 4.1274660577828736E-5 0.0 7 1.238239817334862E-4 0.0 0.0 8.254932115565747E-5 0.0 8 1.238239817334862E-4 0.0 0.0 8.254932115565747E-5 0.0 9 1.238239817334862E-4 0.0 0.0 1.238239817334862E-4 0.0 10-11 1.238239817334862E-4 0.0 0.0 1.8573597260022933E-4 0.0 12-13 1.238239817334862E-4 0.0 0.0 2.0637330288914368E-4 0.0 14-15 1.238239817334862E-4 0.0 0.0 2.0637330288914368E-4 0.0 16-17 1.238239817334862E-4 0.0 0.0 2.0637330288914368E-4 0.0 18-19 1.238239817334862E-4 0.0 0.0 2.2701063317805804E-4 0.0 20-21 1.238239817334862E-4 0.0 0.0 2.889226240448012E-4 0.0 22-23 1.238239817334862E-4 0.0 0.0 3.5083461491154427E-4 0.0 24-25 1.238239817334862E-4 0.0 0.0 4.5402126635611613E-4 0.0 26-27 1.238239817334862E-4 0.0 0.0 5.159332572228592E-4 0.0 28-29 1.238239817334862E-4 0.0 0.0 8.254932115565747E-4 0.0 30-31 1.6509864231131495E-4 0.0 0.0 0.0022701063317805805 0.0 32-33 1.6509864231131495E-4 0.0 0.0 0.0067071323438971705 0.0 34-35 2.0637330288914368E-4 0.0 0.0 0.013703187311839142 0.0 36-37 2.0637330288914368E-4 0.0 0.0 0.025033081640453127 0.0 38-39 2.0637330288914368E-4 0.0 0.0 0.042327164422563374 0.0 40-41 2.0637330288914368E-4 0.0 0.0 0.0652139637129694 0.0 42-43 2.0637330288914368E-4 0.0 0.0 0.08708953381921863 0.0 44-45 2.0637330288914368E-4 0.0 0.0 0.11309256998325073 0.0 46-47 2.0637330288914368E-4 2.0637330288914368E-5 0.0 0.14590592514262457 0.0 48-49 2.0637330288914368E-4 4.1274660577828736E-5 0.0 0.18113384794580142 0.0 50-51 2.0637330288914368E-4 4.1274660577828736E-5 0.0 0.21863187708075882 0.0 52-53 2.0637330288914368E-4 4.1274660577828736E-5 0.0 0.25932869241049794 0.0 54-55 2.0637330288914368E-4 8.254932115565747E-5 0.0 0.3057833228908442 0.0 56-57 2.0637330288914368E-4 8.254932115565747E-5 0.0 0.3578719445400641 0.0 58-59 2.476479634669724E-4 1.0318665144457184E-4 0.0 0.4170804451389594 0.0 60-61 2.889226240448012E-4 1.238239817334862E-4 0.0 0.4794258199417697 0.0 62-63 2.889226240448012E-4 1.238239817334862E-4 0.0 0.5456716501691848 0.0 64-65 2.889226240448012E-4 1.238239817334862E-4 0.0 0.6207089830996775 0.0 66-67 2.889226240448012E-4 1.238239817334862E-4 0.0 0.7150215825200161 0.0 68-69 2.889226240448012E-4 2.0637330288914368E-4 0.0 0.8179193113405432 0.0 70-71 2.889226240448012E-4 2.0637330288914368E-4 0.0 0.9411448104956508 0.0 72-73 2.889226240448012E-4 2.0637330288914368E-4 0.0 1.0688486103234531 0.0 74-75 2.889226240448012E-4 2.0637330288914368E-4 0.0 1.2008243375210603 0.0 76-77 2.889226240448012E-4 2.0637330288914368E-4 0.0 1.3503830701248227 0.0 78-79 2.889226240448012E-4 2.0637330288914368E-4 0.0 1.5157912723904716 0.0 80-81 2.889226240448012E-4 2.0637330288914368E-4 0.0 1.7039624499647927 0.0 82-83 2.889226240448012E-4 2.0637330288914368E-4 0.0 1.9139679229847855 0.0 84-85 2.889226240448012E-4 2.0637330288914368E-4 0.0 2.127007083557248 0.0 86-87 2.889226240448012E-4 2.0637330288914368E-4 0.0 2.363056867401851 0.0 88 2.889226240448012E-4 2.0637330288914368E-4 0.0 2.5661281974447685 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 455 3.6379788E-12 20.107496 1 ACATCGG 415 1.6570993E-9 17.336813 3 TACATCG 375 1.109307E-6 16.26329 2 GTATAAG 950 0.0 15.408693 1 GTATATA 1575 0.0 15.102964 1 GTATTAA 1460 0.0 15.039306 1 GTCTAGA 860 0.0 14.893576 1 GTCCTAG 755 1.8189894E-12 14.541316 1 GTACATG 10655 0.0 14.482554 1 GTCTAAT 845 0.0 14.4361515 1 GTATAAT 1100 0.0 14.416466 1 GTACATC 725 1.8189894E-11 14.301745 1 TACATGG 10815 0.0 14.097859 2 GTATTGC 650 7.930794E-10 14.075249 1 GTATAGG 700 1.6189006E-10 13.941198 1 GTATATG 1325 0.0 13.809677 1 CCGTGCA 405 1.2429664E-6 13.627176 9 GTATTAG 900 0.0 13.553942 1 GTGTATA 1230 0.0 13.38865 1 GTGTAAT 1110 0.0 13.187618 1 >>END_MODULE