FastQCFastQC Report
Wed 25 May 2016
SRR1295106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422794
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT81190.3351089692313915No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59690.24636844898905974No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24670.10182458764550349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG1857.138644E-620.3396975
ACCGTCC1909.115085E-619.7725478
CTAGTAC5600.015.958163
TACACCG2155.161462E-415.3139015
GAGTACT28200.015.07043212-13
TCTACAC10500.014.3343483
GTATAAG9400.014.1754181
AGGACCG2000.005635780314.1106655
CCTAGTA7100.013.8900622
CCTTATA11450.013.7808842
CATGGGG32650.013.3972924
CCTACAC9150.013.3650163
GTACATG139600.013.1509611
CTAATAC11300.012.9032923
ATACCGT2550.001895293212.8930546
TACATGG139950.012.6841742
CACCGTC2600.002195824612.64302357
ACATGGG135850.012.6371883
CCCTACA9800.012.5790112
GTATTAG11000.012.5621871
Produced by FastQC (version 0.11.5)