FastQCFastQC Report
Wed 25 May 2016
SRR1295105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036885
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG4750.020.7907712
GTACATG114050.018.403091
GTATAGA11500.017.1774711
TACATGG118050.017.1495252
GTACGAC2000.001783202416.7718523
GTATTAT16300.016.6637291
GTATAAT14600.016.0671121
ACATGGG120800.015.1335453
GTCCTAT7351.8189894E-1215.1179281
TATACTG14700.015.0631725
GGACCGT3152.365782E-514.3753726
CATGGGG43600.014.2329954
GTATTAA15650.014.2002261
GTACATA15350.014.0755961
GTATTAG10750.013.7819261
TACGACC2850.001179022113.7313434
TACACCG4452.3402572E-713.5707045
AGTGCGC2650.001501685413.2980238
CATGGGT21900.013.2745274
ATAATAC15250.013.1975243
Produced by FastQC (version 0.11.5)