##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295105_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3036885 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.66350619137702 27.0 22.0 33.0 6.0 33.0 2 21.939377026130394 27.0 6.0 33.0 2.0 33.0 3 24.11367667857031 27.0 15.0 33.0 6.0 33.0 4 24.20450889645146 33.0 6.0 37.0 2.0 37.0 5 26.201217695105345 33.0 15.0 37.0 2.0 37.0 6 26.929593316836165 33.0 15.0 37.0 2.0 37.0 7 26.480057032123376 33.0 15.0 37.0 6.0 37.0 8 27.231307079458063 33.0 15.0 37.0 6.0 37.0 9 28.230042625914383 33.0 22.0 37.0 15.0 37.0 10-11 27.833933290197027 33.0 18.5 37.0 6.0 37.0 12-13 26.641149730727374 33.0 15.0 37.0 4.0 37.0 14-15 28.231496912132002 33.0 15.0 37.0 6.0 40.0 16-17 28.640691860244957 33.0 22.0 37.0 6.0 40.0 18-19 28.79391646374492 33.0 22.0 37.0 6.0 40.0 20-21 28.292174217989817 33.0 18.5 37.0 6.0 40.0 22-23 29.03474020254307 33.0 22.0 37.0 6.0 40.0 24-25 29.134023020298763 33.0 22.0 37.0 6.0 40.0 26-27 27.772980372980868 33.0 15.0 37.0 4.0 40.0 28-29 28.472537320313414 33.0 22.0 37.0 6.0 40.0 30-31 29.182774948672737 33.0 22.0 37.0 6.0 40.0 32-33 27.973255819696824 33.0 18.5 37.0 6.0 40.0 34-35 27.49308600753733 33.0 15.0 37.0 6.0 40.0 36-37 27.72948185393915 33.0 15.0 37.0 6.0 40.0 38-39 27.88298322129419 33.0 18.5 37.0 6.0 40.0 40-41 27.000314137677258 33.0 15.0 37.0 6.0 40.0 42-43 27.551974638486477 33.0 15.0 37.0 6.0 40.0 44-45 27.342501938664125 33.0 15.0 37.0 6.0 40.0 46-47 27.118849577774597 33.0 15.0 37.0 6.0 40.0 48-49 26.922712252851195 33.0 15.0 37.0 6.0 40.0 50-51 26.801080877280505 33.0 15.0 37.0 6.0 40.0 52-53 27.046051134633018 33.0 18.5 37.0 6.0 40.0 54-55 26.990625920968363 33.0 22.0 37.0 6.0 40.0 56-57 26.941480168001092 33.0 18.5 37.0 6.0 40.0 58-59 26.795536380205377 33.0 15.0 37.0 6.0 40.0 60-61 26.783011704427402 33.0 18.5 37.0 6.0 40.0 62-63 26.713997731227888 27.0 22.0 37.0 6.0 38.5 64-65 26.26317707124241 27.0 15.0 37.0 6.0 37.0 66-67 26.487154765491614 27.0 18.5 37.0 6.0 37.0 68-69 26.079692513875237 27.0 18.5 37.0 6.0 37.0 70-71 26.198374979625505 27.0 18.5 37.0 6.0 37.0 72-73 26.21725765052019 27.0 22.0 33.0 6.0 37.0 74-75 26.34680058678547 27.0 22.0 33.0 6.0 37.0 76-77 26.11049018978328 27.0 22.0 33.0 6.0 37.0 78-79 25.717763431937662 27.0 18.5 33.0 6.0 37.0 80-81 25.526318744371288 27.0 22.0 33.0 6.0 37.0 82-83 25.625643052008883 27.0 22.0 33.0 6.0 37.0 84-85 25.559662450175097 27.0 22.0 33.0 6.0 37.0 86-87 25.365508736748346 27.0 22.0 33.0 6.0 37.0 88-89 25.32845711971313 27.0 22.0 33.0 6.0 37.0 90-91 25.45937020993551 27.0 22.0 33.0 6.0 37.0 92-93 25.39851723064917 27.0 22.0 33.0 6.0 37.0 94-95 25.277747264055108 27.0 22.0 33.0 6.0 37.0 96-97 25.356099753530344 27.0 22.0 33.0 6.0 37.0 98-99 25.42322857138153 27.0 22.0 33.0 6.0 37.0 100 25.101943274111466 27.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.241372801620461 1101 2 11.243664842093512 1101 3 8.352458336926933 1101 4 12.598413663179866 1101 5 10.703261238132594 1101 6 9.892602869241266 1101 7 9.447087172560689 1101 8 9.066853902705134 1101 9 7.519738292287588 1101 10-11 7.755848603817693 1101 12-13 9.073206870505413 1101 14-15 7.841182413035572 1101 16-17 8.075850378173065 1101 18-19 8.107336485901978 1101 20-21 7.157675282420051 1101 22-23 7.246374290028768 1101 24-25 7.3777067459874495 1101 26-27 8.354494074352193 1101 28-29 8.000617925214339 1101 30-31 5.3983670819952785 1101 32-33 6.793607522289051 1101 34-35 8.615165394290052 1101 36-37 8.079461951015489 1101 38-39 8.024687721192155 1101 40-41 8.848354740356644 1101 42-43 8.43115382730496 1101 44-45 8.28463397018622 1101 46-47 7.094626588148888 1101 48-49 5.207770147212351 1101 50-51 6.543642883518654 1101 52-53 5.609926476474602 1101 54-55 4.001054372738583 1101 56-57 6.024937394004851 1101 58-59 5.845080680278603 1101 60-61 6.494367223436271 1101 62-63 6.126432767207838 1101 64-65 6.485085096103582 1101 66-67 5.592804081767866 1101 68-69 6.440074105960406 1101 70-71 6.0306135870890465 1101 72-73 5.287591227782315 1101 74-75 4.732495523861189 1101 76-77 4.868534697393073 1101 78-79 4.953812318556658 1101 80-81 4.710837080106128 1101 82-83 4.203852590427502 1101 84-85 4.033300130826731 1101 86-87 3.9166336863786384 1101 88-89 3.787798622172037 1101 90-91 3.5920489569223797 1101 92-93 3.5013075709834496 1101 94-95 3.4265070306851726 1101 96-97 3.640836578852518 1101 98-99 3.311813198281257 1101 100 3.6171486384368343 1102 1 6.704854113755545 1102 2 10.366512275829493 1102 3 7.940825778909819 1102 4 12.178487227319284 1102 5 10.325295679192816 1102 6 9.33594104495004 1102 7 6.538232826924748 1102 8 7.662477779539664 1102 9 6.3860304242438986 1102 10-11 7.26740039262091 1102 12-13 8.928892811142322 1102 14-15 5.947485957595905 1102 16-17 7.121597483348879 1102 18-19 7.20403792530843 1102 20-21 6.830849667886785 1102 22-23 6.341148070430087 1102 24-25 6.8531029336951725 1102 26-27 8.24999463145689 1102 28-29 7.475505809722588 1102 30-31 4.1860160636342165 1102 32-33 4.457396759055619 1102 34-35 5.026463287381517 1102 36-37 4.887450737272989 1102 38-39 4.400358239094807 1102 40-41 5.922953398333842 1102 42-43 6.65313793154349 1102 44-45 6.374223003119734 1102 46-47 5.321328627064329 1102 48-49 3.5810458277647186 1102 50-51 4.858576652397705 1102 52-53 5.603916216097613 1102 54-55 5.177654915513553 1102 56-57 6.0188409542109085 1102 58-59 5.745315530613375 1102 60-61 5.805361414047962 1102 62-63 4.195251281215146 1102 64-65 5.824619295897989 1102 66-67 3.5746077045568114 1102 68-69 5.77619987782823 1102 70-71 4.957675276563858 1102 72-73 4.669629975071732 1102 74-75 3.906162310917061 1102 76-77 3.036062457583629 1102 78-79 4.280391640906792 1102 80-81 4.251002994218425 1102 82-83 3.5848330581322436 1102 84-85 2.704812341070145 1102 86-87 2.946990683014075 1102 88-89 3.2328213633025946 1102 90-91 3.102072728915953 1102 92-93 2.9505787837316433 1102 94-95 2.8269121379168567 1102 96-97 3.1280766332177485 1102 98-99 2.8378728304166927 1102 100 2.972824160777318 1103 1 6.512375576596149 1103 2 10.754082802381074 1103 3 7.747158412964609 1103 4 11.560269639690247 1103 5 10.201621471518141 1103 6 8.89098325587868 1103 7 7.875546668881903 1103 8 7.831453299632685 1103 9 7.072814059799672 1103 10-11 7.359919724098827 1103 12-13 8.627822773127807 1103 14-15 8.279228275663744 1103 16-17 7.838851177403825 1103 18-19 7.096808000460609 1103 20-21 8.258163799060831 1103 22-23 6.702169085260834 1103 24-25 4.9972131238561985 1103 26-27 5.630349334786683 1103 28-29 6.808768680979444 1103 30-31 5.9426528543804515 1103 32-33 7.590633637635367 1103 34-35 6.862520325373193 1103 36-37 4.022320355720385 1103 38-39 6.099757994310476 1103 40-41 6.40404598237069 1103 42-43 7.263837399826528 1103 44-45 5.385693722580825 1103 46-47 5.979439723929513 1103 48-49 5.300409607715245 1103 50-51 6.119958073841605 1103 52-53 5.709403563135037 1103 54-55 4.564675193117555 1103 56-57 5.589806581244176 1103 58-59 5.246970882820598 1103 60-61 4.72973428314582 1103 62-63 2.537919458072931 1103 64-65 4.703099615886082 1103 66-67 3.6365712256490106 1103 68-69 4.797966718540831 1103 70-71 3.3564872214792807 1103 72-73 4.158753485788967 1103 74-75 3.14998370788253 1103 76-77 3.2487127214157496 1103 78-79 2.728868711349712 1103 80-81 3.8166171416770354 1103 82-83 3.2195603708173337 1103 84-85 2.9945221423173507 1103 86-87 2.647946084296059 1103 88-89 2.8109277921703466 1103 90-91 2.5604168848558047 1103 92-93 2.2359634569312945 1103 94-95 2.6310353483643283 1103 96-97 2.716973431052562 1103 98-99 2.578771478871168 1103 100 2.7498933878792613 1104 1 6.151157194713999 1104 2 9.555508061482975 1104 3 7.127463122986388 1104 4 11.729527139450692 1104 5 9.245299093373013 1104 6 7.938640081176281 1104 7 3.9542133983774868 1104 8 6.285699814091654 1104 9 6.465955456369379 1104 10-11 6.972874521574557 1104 12-13 7.210820039140511 1104 14-15 6.991879621554869 1104 16-17 6.914700724733564 1104 18-19 6.589152422447125 1104 20-21 7.310269209067371 1104 22-23 6.1923569348919045 1104 24-25 5.855473643958799 1104 26-27 7.940762265758426 1104 28-29 6.8711223260836185 1104 30-31 6.145721977248815 1104 32-33 7.37318723019003 1104 34-35 7.830879064917312 1104 36-37 6.438442311480461 1104 38-39 7.084257666085147 1104 40-41 6.968842426904608 1104 42-43 6.989658873969503 1104 44-45 6.317981577165714 1104 46-47 6.3121703155799445 1104 48-49 4.923651641152681 1104 50-51 5.334751307223055 1104 52-53 5.718984310700627 1104 54-55 4.466169048198569 1104 56-57 5.706428215098999 1104 58-59 3.6052934418312184 1104 60-61 4.029031607702805 1104 62-63 3.605106526617888 1104 64-65 2.493205097829879 1104 66-67 2.860338073394935 1104 68-69 3.723598170064463 1104 70-71 2.32616251029944 1104 72-73 2.3263832309870374 1104 74-75 2.96187301383398 1104 76-77 3.433483967929373 1104 78-79 3.995970793561348 1104 80-81 2.846274562826977 1104 82-83 2.681733145783763 1104 84-85 2.698526949008297 1104 86-87 0.5839695176231032 1104 88-89 0.018976048588911 1104 90-91 1.9456364589073516 1104 92-93 2.243996484772964 1104 94-95 2.273207137214186 1104 96-97 2.4976860132029977 1104 98-99 2.1875233494488064 1104 100 0.5531537671744573 1105 1 5.558234138116081 1105 2 -2.565430476911068 1105 3 0.6322082393239477 1105 4 7.88798783633268 1105 5 8.250234777852988 1105 6 7.7292114468988835 1105 7 4.986009928020973 1105 8 5.565187889162075 1105 9 4.778387306095301 1105 10-11 4.793664095422368 1105 12-13 4.760424479839038 1105 14-15 5.4761095873157615 1105 16-17 5.684782623421956 1105 18-19 5.841979460022195 1105 20-21 6.803900825012242 1105 22-23 1.6561602506980506 1105 24-25 3.7425670019046713 1105 26-27 6.558459917693188 1105 28-29 5.6523480752524335 1105 30-31 5.05150022928586 1105 32-33 6.777585553209089 1105 34-35 5.797778756321833 1105 36-37 5.879838069794303 1105 38-39 3.833138385258021 1105 40-41 5.0092810475421246 1105 42-43 5.157781658705488 1105 44-45 5.741990969014985 1105 46-47 4.940542999780529 1105 48-49 4.5109331602860365 1105 50-51 5.554374459949468 1105 52-53 5.221988688692008 1105 54-55 4.998833356439253 1105 56-57 4.798682610727354 1105 58-59 1.610266809541514 1105 60-61 4.111390646691078 1105 62-63 -0.795537391135646 1105 64-65 -0.726842336257917 1105 66-67 0.45691586533634876 1105 68-69 2.51523763665892 1105 70-71 2.7074468965394978 1105 72-73 2.3295721187988896 1105 74-75 2.3589637725492167 1105 76-77 1.501957707279125 1105 78-79 2.984030495961605 1105 80-81 1.382626014924881 1105 82-83 0.7727898181809643 1105 84-85 1.1147544847973094 1105 86-87 -0.6231219844646105 1105 88-89 1.1825777579432781 1105 90-91 1.055102570572469 1105 92-93 1.3668394414661122 1105 94-95 0.8249328443723982 1105 96-97 1.774392102158064 1105 98-99 1.780244937603051 1105 100 1.7610397515203147 1106 1 5.8602545500282375 1106 2 10.065499039172131 1106 3 6.541486443984702 1106 4 11.359036181857093 1106 5 9.635763846541654 1106 6 9.204457035652922 1106 7 6.324203404839629 1106 8 7.0748710724401676 1106 9 6.841783112346182 1106 10-11 5.313019965742463 1106 12-13 7.671705496100838 1106 14-15 7.584392408188716 1106 16-17 7.511788004631349 1106 18-19 6.9265548634066825 1106 20-21 7.987418140604262 1106 22-23 5.91685680733945 1106 24-25 4.665816751060849 1106 26-27 7.3758168250321425 1106 28-29 5.787555664851364 1106 30-31 5.003681312389915 1106 32-33 6.9239211109721275 1106 34-35 6.463217162554809 1106 36-37 5.76178760917405 1106 38-39 6.866088491666222 1106 40-41 6.265285589701403 1106 42-43 5.950570295002983 1106 44-45 4.097635909662358 1106 46-47 5.550188188074202 1106 48-49 5.503676947765477 1106 50-51 6.391245202486115 1106 52-53 5.344885305539631 1106 54-55 4.94524229748998 1106 56-57 4.43205872298082 1106 58-59 4.628330553808901 1106 60-61 4.360716072365587 1106 62-63 4.493776017854621 1106 64-65 4.772257802653861 1106 66-67 2.6641976673561096 1106 68-69 2.9919040983439054 1106 70-71 2.4359608347995696 1106 72-73 2.5266869954456403 1106 74-75 2.529529590112862 1106 76-77 3.1248812019057013 1106 78-79 3.7836168701140025 1106 80-81 3.364883274751996 1106 82-83 1.705924530906632 1106 84-85 2.2204066314699027 1106 86-87 2.96563891470646 1106 88-89 3.189372251928308 1106 90-91 2.8381607557737105 1106 92-93 2.659182671259444 1106 94-95 1.1809283662563814 1106 96-97 1.2089601187874415 1106 98-99 1.6606944072859378 1106 100 2.616094678578655 1107 1 5.9467514588304695 1107 2 10.250557898166516 1107 3 5.892423587394937 1107 4 11.06999942392094 1107 5 9.23866926341167 1107 6 7.819220803771401 1107 7 3.6183173887851083 1107 8 5.923475884831316 1107 9 5.607675927111629 1107 10-11 4.420659792661045 1107 12-13 5.931909282504698 1107 14-15 5.91204134281438 1107 16-17 6.754649725498204 1107 18-19 6.036051799613663 1107 20-21 5.428454180186055 1107 22-23 5.611046883203969 1107 24-25 4.332784811696488 1107 26-27 6.928822817343647 1107 28-29 6.138283704169606 1107 30-31 4.811213047967872 1107 32-33 4.570047834045937 1107 34-35 6.53813032738973 1107 36-37 6.352507893530426 1107 38-39 6.092900765030379 1107 40-41 7.449697323366859 1107 42-43 5.863257645843873 1107 44-45 3.796062748594135 1107 46-47 3.2389043337298773 1107 48-49 3.7971024893446064 1107 50-51 4.52033415772949 1107 52-53 3.5237865032439295 1107 54-55 4.945209575049393 1107 56-57 4.973148988987674 1107 58-59 2.8260029457365086 1107 60-61 2.5495951421899825 1107 62-63 0.1822469434530518 1107 64-65 1.726682999632807 1107 66-67 3.6854933873751676 1107 68-69 4.6317618385246995 1107 70-71 4.041710048662178 1107 72-73 2.1589258995820693 1107 74-75 2.22085989259719 1107 76-77 2.7621670468845956 1107 78-79 3.6527646581131705 1107 80-81 1.9240188355669332 1107 82-83 1.3267866261107422 1107 84-85 2.0981347658301566 1107 86-87 2.0877766953063457 1107 88-89 2.2991905467579663 1107 90-91 1.1845054107682849 1107 92-93 2.060634761629821 1107 94-95 1.9819135496145819 1107 96-97 2.0683386256482486 1107 98-99 1.5346950521722391 1107 100 -0.18687007249581455 1108 1 4.097690636458392 1108 2 -3.0060478069837444 1108 3 0.17003288992090404 1108 4 6.247931250187314 1108 5 6.539909580800462 1108 6 6.513788457897032 1108 7 4.072091140110327 1108 8 4.6964580388437085 1108 9 4.733280683868134 1108 10-11 3.303461821722994 1108 12-13 2.279841906376621 1108 14-15 3.637677910642001 1108 16-17 4.3859820702664045 1108 18-19 3.687478354988162 1108 20-21 4.466697747963025 1108 22-23 3.848455340500923 1108 24-25 2.490870872753483 1108 26-27 4.359684609767097 1108 28-29 5.789375777542329 1108 30-31 4.165776917833579 1108 32-33 4.136341687068775 1108 34-35 2.788623358238432 1108 36-37 1.002832321044675 1108 38-39 2.254487569257801 1108 40-41 5.049126866560112 1108 42-43 2.6242195697849056 1108 44-45 3.2995434060772766 1108 46-47 4.31691543150875 1108 48-49 4.454946357766474 1108 50-51 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-4.892572840739518 2212 86-87 -4.6335207285341795 2212 88-89 -3.0674999657946174 2212 90-91 -4.097349879535276 2212 92-93 -5.378048891881416 2212 94-95 -3.686917097707447 2212 96-97 -5.338989529912666 2212 98-99 -3.773256144141026 2212 100 -3.771909257448794 2213 1 -20.583056970288304 2213 2 -16.61948041600973 2213 3 -19.412097485401997 2213 4 -18.35911935002542 2213 5 -20.645928437737755 2213 6 -21.32453398550063 2213 7 -10.098304749764242 2213 8 -9.859228071634183 2213 9 -4.418261616628211 2213 10-11 -17.058487267022016 2213 12-13 -15.692787534080988 2213 14-15 -22.84841842730056 2213 16-17 -17.29071201354396 2213 18-19 -17.939402271825124 2213 20-21 -17.053027937045172 2213 22-23 -19.09810837457461 2213 24-25 -7.261890732907766 2213 26-27 -22.4907940408939 2213 28-29 -23.364628173225032 2213 30-31 -18.660301507881464 2213 32-33 -22.532097759823795 2213 34-35 -17.124600945806804 2213 36-37 -22.856687508768424 2213 38-39 -10.853167425282335 2213 40-41 -16.203784160165426 2213 42-43 -22.370841971014364 2213 44-45 -16.28326310032404 2213 46-47 -16.102542253293752 2213 48-49 -5.661528505608874 2213 50-51 -22.419742470623003 2213 52-53 -16.99035036649864 2213 54-55 -16.87528670965985 2213 56-57 -16.558890767930336 2213 58-59 -17.154702121240238 2213 60-61 -18.318078921219904 2213 62-63 -16.62595081368337 2213 64-65 -16.73055585718661 2213 66-67 -8.193309721441096 2213 68-69 -21.759655785996376 2213 70-71 -16.509114728966402 2213 72-73 -8.909761559177511 2213 74-75 -4.074145267742487 2213 76-77 -2.647361785648158 2213 78-79 -4.118147637645819 2213 80-81 -3.03308069977178 2213 82-83 -3.0556807391628666 2213 84-85 -2.7680265720744224 2213 86-87 -2.9733240028391634 2213 88-89 -2.524068788381104 2213 90-91 -2.376844521785845 2213 92-93 -3.4102887315279204 2213 94-95 -3.618501307856988 2213 96-97 -3.13171317770205 2213 98-99 -3.525487428513017 2213 100 -3.0808281837653517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 818.0 3 818.0 4 4209.0 5 13896.0 6 22556.0 7 27445.0 8 27048.0 9 28074.0 10 32967.0 11 41177.0 12 53871.0 13 70088.0 14 62963.0 15 69084.0 16 75187.0 17 76863.0 18 75790.0 19 73868.0 20 71839.0 21 70839.0 22 74169.0 23 78493.0 24 84807.0 25 90777.0 26 97323.0 27 103070.0 28 109560.0 29 114040.0 30 119569.0 31 129995.0 32 146461.0 33 169484.0 34 195043.0 35 213429.0 36 212382.0 37 158790.0 38 40051.0 39 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.55200593108431 20.8804336623982 13.996317678175913 24.571242728341574 2 14.778379185158846 21.0553163095536 40.58169702237499 23.584607482912567 3 17.525393413272607 27.34991037618845 29.641817007438302 25.482879203100644 4 12.706938456317921 18.088070388788505 38.76843735304807 30.436553801845502 5 13.127159910698206 37.62961352016785 36.18436825927574 13.058858309858199 6 29.937655426832503 38.96940230091184 18.448540566060178 12.64440170619548 7 26.23550307847396 31.869300835416425 23.689017007948923 18.206179078160687 8 24.90221299554761 35.86074019903221 21.649197167521482 17.587849637898696 9 24.91595414877325 17.34544210675398 22.02772077673295 35.71088296773983 10-11 23.42235714818113 27.40301244889791 28.98596288821779 20.188667514703166 12-13 24.38003945115989 24.958991364489812 28.77899595166344 21.881973232686857 14-15 21.74370675957791 26.484762983704908 26.347515835524298 25.42401442119288 16-17 21.447093003418 29.932352802989932 27.20218717659818 21.418367016993887 18-19 21.475061496451257 29.084171857192825 28.53232583527978 20.908440811076133 20-21 22.67018017466602 28.386807307208954 27.680526338961176 21.262486179163854 22-23 21.774247115845107 28.45125332357958 28.032575133252635 21.74192442732268 24-25 21.988782496064072 28.441445432368162 27.571344183559283 21.998427888008486 26-27 22.008362927294115 28.302797125612567 27.662755249050075 22.026084698043245 28-29 21.838784494773368 28.146874426422713 28.223260650205017 21.791080428598907 30-31 22.440913630304614 28.539485922223985 27.502569531655176 21.51703091581623 32-33 21.513497034532453 28.501098148013508 28.092397043674687 21.89300777377935 34-35 21.601093012451546 28.249282732743914 27.82684866990811 22.322775584896426 36-37 22.308047455671247 28.55959594922057 27.409473672466188 21.722882922642 38-39 22.0653898277399 28.370668860003885 27.630977192242707 21.93296412001351 40-41 22.0692158886591 28.304326964666338 27.27977735545666 22.346679791217905 42-43 21.983681439871297 28.374074044112447 27.76443182773431 21.877812688281946 44-45 22.397522831277087 27.91237826381875 27.70944725701962 21.980651647884542 46-47 22.16493354075306 28.282520031189645 27.904622710926592 21.647923717130706 48-49 22.142841476570972 28.11313829590364 27.852497803292938 21.891522424232456 50-51 22.301951541594555 28.068287638719518 27.630601546719003 21.999159272966924 52-53 21.855069498938477 28.15143103978829 27.693517593901788 22.299981867371446 54-55 22.255475181396587 28.17567108416358 27.480300164199967 22.088553570239867 56-57 22.345998866031586 27.999255562721004 27.435439542603884 22.219306028643523 58-59 22.1398145194943 27.68456020603785 27.789771458348845 22.385853816119006 60-61 22.47444608161816 28.059480293593463 27.44836470360199 22.017708921186383 62-63 22.052423336502514 28.159780436205327 27.71828105769564 22.069515169596517 64-65 22.023817032165624 27.790385096220056 27.780345704133758 22.40545216748056 66-67 22.18414323634152 27.960967508649336 27.497962127177466 22.35692712783168 68-69 22.685771948648842 27.802243909501335 27.428062252856535 22.083921888993295 70-71 22.600675435214065 27.850931542797486 27.311079717157284 22.237313304831165 72-73 22.387638442719233 27.990729969712792 27.580414685288595 22.041216902279377 74-75 22.566695505904747 27.689797924669392 27.28510331462183 22.45840325480404 76-77 22.474992888381696 27.992078239446112 27.4219177580852 22.111011114086992 78-79 22.569835393528688 27.745999874505866 27.55581769885591 22.128347033109534 80-81 22.606373995445633 27.71098813549097 27.397969978017983 22.284667891045416 82-83 22.133889382757296 27.902603846605135 27.666027620833688 22.297479149803877 84-85 22.303353069153744 27.678114419359595 27.304017442207034 22.714515069279624 86-87 22.552574952477304 27.739122918046426 27.393600976618053 22.314701152858213 88-89 22.41460084407611 27.43972688830358 27.67759836583421 22.468073901786106 90-91 22.619812534630405 27.520623137713425 27.399443537467498 22.460120790188675 92-93 22.160792033444345 27.805573782669523 27.681288251146245 22.352345932739887 94-95 22.096751246530065 27.423661407032185 27.666317053722384 22.81327029271537 96-97 22.352647774222707 27.528696618629628 27.6000223741117 22.518633233035974 98-99 22.280639852169852 27.386689400270036 27.621741455305553 22.71092929225456 100 22.450750096513396 27.21621676067022 27.46119071608813 22.87184242672825 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 313.0 1 368.0 2 352.0 3 268.0 4 310.0 5 437.0 6 607.0 7 856.0 8 1272.0 9 1863.0 10 2651.0 11 3515.0 12 4392.0 13 5356.5 14 6251.0 15 7154.5 16 8103.0 17 9049.0 18 10109.5 19 11038.0 20 11942.5 21 13056.0 22 14392.5 23 15825.5 24 17771.5 25 20610.5 26 24281.0 27 29184.0 28 35432.5 29 42758.5 30 51289.0 31 60448.0 32 69507.0 33 78023.0 34 86081.5 35 93849.5 36 100099.0 37 105753.0 38 110805.0 39 113789.0 40 114841.0 41 114774.0 42 113448.5 43 110605.0 44 107211.5 45 103371.5 46 99955.0 47 96847.5 48 93150.0 49 90421.5 50 89448.5 51 85217.0 52 77111.0 53 69650.0 54 63403.0 55 58547.0 56 54004.5 57 48838.0 58 44051.5 59 39736.5 60 34948.5 61 30000.5 62 25460.5 63 21120.0 64 17366.5 65 14244.5 66 11422.5 67 8991.5 68 7001.5 69 5493.0 70 4217.0 71 3222.5 72 2542.0 73 1951.5 74 1441.0 75 1053.0 76 793.0 77 591.5 78 437.5 79 296.0 80 200.5 81 145.0 82 94.0 83 59.5 84 42.0 85 29.0 86 19.0 87 14.0 88 11.0 89 7.5 90 5.0 91 4.5 92 4.5 93 3.0 94 1.5 95 2.0 96 1.0 97 0.5 98 0.5 99 1.0 100 3.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.661666477327921 2 24.98925708415037 3 6.145705220974781 4 23.533884226765252 5 12.78737917306714 6 10.026392174876559 7 8.342857895508061 8 8.685412849021283 9 5.372478707623108 10-11 6.784320117488808 12-13 10.19845993509797 14-15 7.570421665621188 16-17 7.837817368784132 18-19 6.616944665339649 20-21 8.004781214962042 22-23 5.562624202101825 24-25 5.264061694795819 26-27 9.60438080467321 28-29 6.261926283016973 30-31 3.9677992416571586 32-33 8.514349407369723 34-35 7.1505835749460385 36-37 7.032749017496546 38-39 5.85875000205803 40-41 8.073091341950715 42-43 6.379629126555665 44-45 6.3815554425011145 46-47 5.531457397958764 48-49 3.91457035745509 50-51 6.665826990485316 52-53 4.388691043618708 54-55 5.024786911588683 56-57 4.899675160567489 58-59 3.5724434741519686 60-61 4.016286425070426 62-63 3.0939268362153984 64-65 4.718107534529625 66-67 2.606091439089725 68-69 5.1565831435829805 70-71 2.477505733671179 72-73 1.5914168630027148 74-75 0.720985483480606 76-77 1.0286856433483653 78-79 1.6035345427963192 80-81 2.025051985834169 82-83 0.6548486360201324 84-85 1.121346379596198 86-87 0.38695900569168734 88-89 0.5797881711029559 90-91 0.219698803214478 92-93 0.2625222884633432 94-95 0.4772982842616695 96-97 0.5115768295473816 98-99 0.4239212219099505 100 0.29003403158170293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3036885.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.17576719980903 #Duplication Level Percentage of deduplicated Percentage of total 1 97.2892012691973 92.59574371052487 2 2.079747394657808 3.958831077367217 3 0.3321858367861703 0.9484812560710291 4 0.10927030701387352 0.41599541208816343 5 0.05746797357367231 0.27347792371463064 6 0.03434326344691643 0.19611878680232575 7 0.024497019036030045 0.163206580660374 8 0.013598153428922987 0.10353717480787673 9 0.010699164421587578 0.09164710639993488 >10 0.04683689240002849 0.7123156653926375 >50 8.60312637316739E-4 0.055010054586609396 >100 0.0010141168708691323 0.20526944981194112 >500 2.0872239742027678E-4 0.13428952458626217 >1k 6.957413214995154E-5 0.14607627718619373 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 3.292847770001169E-5 0.0 0.0 0.0 2 0.0 6.585695540002338E-5 0.0 0.0 0.0 3 3.292847770001169E-5 6.585695540002338E-5 0.0 0.0 0.0 4 3.292847770001169E-5 6.585695540002338E-5 0.0 0.0 0.0 5 3.292847770001169E-5 9.878543310003507E-5 0.0 0.0 0.0 6 3.292847770001169E-5 9.878543310003507E-5 0.0 0.0 0.0 7 3.292847770001169E-5 9.878543310003507E-5 0.0 0.0 0.0 8 3.292847770001169E-5 9.878543310003507E-5 0.0 0.0 0.0 9 3.292847770001169E-5 9.878543310003507E-5 0.0 0.0 0.0 10-11 3.292847770001169E-5 9.878543310003507E-5 0.0 0.0 0.0 12-13 3.292847770001169E-5 9.878543310003507E-5 0.0 0.0 0.0 14-15 6.585695540002338E-5 1.3171391080004676E-4 0.0 1.6464238850005844E-5 0.0 16-17 6.585695540002338E-5 1.3171391080004676E-4 0.0 3.292847770001169E-5 0.0 18-19 6.585695540002338E-5 1.3171391080004676E-4 0.0 6.585695540002338E-5 0.0 20-21 6.585695540002338E-5 1.3171391080004676E-4 0.0 6.585695540002338E-5 0.0 22-23 6.585695540002338E-5 1.3171391080004676E-4 0.0 8.232119425002923E-5 0.0 24-25 6.585695540002338E-5 1.3171391080004676E-4 0.0 1.3171391080004676E-4 0.0 26-27 8.232119425002923E-5 1.3171391080004676E-4 0.0 1.3171391080004676E-4 0.0 28-29 9.878543310003507E-5 1.481781496500526E-4 0.0 4.2807021010015197E-4 0.0 30-31 9.878543310003507E-5 1.9757086620007013E-4 0.0 0.001481781496500526 0.0 32-33 9.878543310003507E-5 1.9757086620007013E-4 0.0 0.004050202757101438 0.0 34-35 1.3171391080004676E-4 1.9757086620007013E-4 0.0 0.008512011485453021 0.0 36-37 1.3171391080004676E-4 2.304993439000818E-4 0.0 0.01608556135645571 0.0 38-39 1.481781496500526E-4 2.634278216000935E-4 0.0 0.02868070407671018 0.0 40-41 1.6464238850005846E-4 2.634278216000935E-4 0.0 0.0439265892518156 0.0 42-43 1.6464238850005846E-4 2.963562993001052E-4 0.0 0.060341435385271425 0.0 44-45 1.6464238850005846E-4 2.963562993001052E-4 0.0 0.08013145048297844 0.0 46-47 1.6464238850005846E-4 2.963562993001052E-4 0.0 0.10436681007018705 0.0 48-49 1.6464238850005846E-4 2.963562993001052E-4 0.0 0.13067666375249637 0.0 50-51 1.811066273500643E-4 2.963562993001052E-4 0.0 0.16090500628110713 0.0 52-53 2.3049934390008184E-4 2.963562993001052E-4 0.0 0.19384994821996882 0.0 54-55 2.3049934390008184E-4 3.292847770001169E-4 0.0 0.22939623989713143 0.0 56-57 2.3049934390008184E-4 3.292847770001169E-4 0.0 0.26975008931849576 0.0 58-59 2.3049934390008184E-4 3.292847770001169E-4 1.6464238850005844E-5 0.31530663821646193 0.0 60-61 2.3049934390008184E-4 3.292847770001169E-4 3.292847770001169E-5 0.36606588659102995 0.0 62-63 2.3049934390008184E-4 3.292847770001169E-4 3.292847770001169E-5 0.4212046224996995 0.0 64-65 2.3049934390008184E-4 3.292847770001169E-4 3.292847770001169E-5 0.4811179876748708 0.0 66-67 2.3049934390008184E-4 3.292847770001169E-4 3.292847770001169E-5 0.5514696802809458 0.0 68-69 2.3049934390008184E-4 3.292847770001169E-4 3.292847770001169E-5 0.6334780539928249 0.0 70-71 2.3049934390008184E-4 3.4574901585012277E-4 3.292847770001169E-5 0.7314567393892097 0.0 72-73 2.3049934390008184E-4 3.6221325470012857E-4 3.292847770001169E-5 0.8355271931600966 0.0 74-75 2.3049934390008184E-4 3.6221325470012857E-4 3.292847770001169E-5 0.9429563516563848 0.0 76-77 2.3049934390008184E-4 3.6221325470012857E-4 3.292847770001169E-5 1.0626349038570773 0.0 78-79 2.3049934390008184E-4 3.6221325470012857E-4 3.292847770001169E-5 1.2013625804072263 0.0 80-81 2.3049934390008184E-4 3.786774935501344E-4 3.292847770001169E-5 1.360555305847933 0.0 82-83 2.3049934390008184E-4 3.9514173240014027E-4 3.292847770001169E-5 1.5313059269613436 0.0 84-85 2.3049934390008184E-4 3.9514173240014027E-4 3.292847770001169E-5 1.7052835388893555 0.0 86-87 2.3049934390008184E-4 3.9514173240014027E-4 3.292847770001169E-5 1.8967791009537733 0.0 88 2.3049934390008184E-4 3.9514173240014027E-4 3.292847770001169E-5 2.05661393170963 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATCG 475 0.0 20.790771 2 GTACATG 11405 0.0 18.40309 1 GTATAGA 1150 0.0 17.177471 1 TACATGG 11805 0.0 17.149525 2 GTACGAC 200 0.0017832024 16.771852 3 GTATTAT 1630 0.0 16.663729 1 GTATAAT 1460 0.0 16.067112 1 ACATGGG 12080 0.0 15.133545 3 GTCCTAT 735 1.8189894E-12 15.117928 1 TATACTG 1470 0.0 15.063172 5 GGACCGT 315 2.365782E-5 14.375372 6 CATGGGG 4360 0.0 14.232995 4 GTATTAA 1565 0.0 14.200226 1 GTACATA 1535 0.0 14.075596 1 GTATTAG 1075 0.0 13.781926 1 TACGACC 285 0.0011790221 13.731343 4 TACACCG 445 2.3402572E-7 13.570704 5 AGTGCGC 265 0.0015016854 13.298023 8 CATGGGT 2190 0.0 13.274527 4 ATAATAC 1525 0.0 13.197524 3 >>END_MODULE