FastQCFastQC Report
Wed 25 May 2016
SRR1295105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036885
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT75340.24808315099188807No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55950.1842348327315654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG16650.015.2482795
CATGGGG47500.014.748034
TAGACAG17100.014.5720645
CCTATAC9050.014.0267323
GTATATA22700.013.9875121
GTATCAA118250.013.9710631
TATACAG17900.013.9207995
GAGTACT31600.013.89130812-13
ATTATAC15850.013.6448853
CAACGCA126650.013.25275
AACGCAG127300.013.1305556
TCTATAC13650.013.08857253
CTAGCAC12600.013.059873
TCAACGC128500.013.0253134
ATCAACG130300.012.9536273
GTACATG147850.012.8182381
GTCTCGC4654.7510912E-712.8030851
GAACCGT2953.9176337E-412.7329636
AGTACTT38500.012.68210112-13
GTATAAC7851.8189894E-1212.6399891
Produced by FastQC (version 0.11.5)