##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295105_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3036885 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.322753742733095 33.0 33.0 33.0 27.0 33.0 2 30.06562546820179 33.0 33.0 33.0 27.0 33.0 3 30.886989793818337 33.0 33.0 33.0 27.0 33.0 4 35.1233721395443 37.0 37.0 37.0 33.0 37.0 5 35.45573638777893 37.0 37.0 37.0 33.0 37.0 6 35.22978710092743 37.0 37.0 37.0 33.0 37.0 7 35.11535800664167 37.0 37.0 37.0 33.0 37.0 8 35.10848451620657 37.0 37.0 37.0 33.0 37.0 9 35.06564851813618 37.0 37.0 37.0 33.0 37.0 10-11 35.04706549638857 37.0 37.0 37.0 33.0 37.0 12-13 34.96384535469733 37.0 37.0 37.0 33.0 37.0 14-15 36.4204988005802 37.0 37.0 40.0 33.0 40.0 16-17 36.407188780609076 37.0 37.0 40.0 33.0 40.0 18-19 36.41735758845 38.5 37.0 40.0 33.0 40.0 20-21 36.40992250282773 40.0 37.0 40.0 33.0 40.0 22-23 36.37539650003211 38.5 37.0 40.0 33.0 40.0 24-25 36.34907248710438 37.0 37.0 40.0 33.0 40.0 26-27 36.33268645338892 37.0 37.0 40.0 33.0 40.0 28-29 36.27839694292013 37.0 37.0 40.0 30.0 40.0 30-31 36.18622848741391 37.0 37.0 40.0 27.0 40.0 32-33 36.150410371153335 37.0 37.0 40.0 27.0 40.0 34-35 36.05321241996322 37.0 37.0 40.0 27.0 40.0 36-37 36.0490708406805 37.0 37.0 40.0 27.0 40.0 38-39 35.983279906878266 37.0 37.0 40.0 27.0 40.0 40-41 35.91914741585539 37.0 37.0 40.0 27.0 40.0 42-43 35.82968502264656 37.0 37.0 40.0 27.0 40.0 44-45 35.73239454243411 37.0 37.0 40.0 27.0 40.0 46-47 35.636086483353836 37.0 37.0 40.0 27.0 40.0 48-49 35.53397724971475 37.0 37.0 40.0 27.0 40.0 50-51 35.43512991107664 37.0 37.0 40.0 27.0 40.0 52-53 35.33508825655235 37.0 33.0 40.0 27.0 40.0 54-55 35.20805068351287 37.0 33.0 40.0 27.0 40.0 56-57 35.08108077849507 37.0 33.0 40.0 27.0 40.0 58-59 34.94815608757 37.0 33.0 40.0 27.0 40.0 60-61 34.784155475100306 37.0 33.0 40.0 27.0 40.0 62-63 34.594759268131654 37.0 33.0 40.0 27.0 40.0 64-65 34.41150274047256 37.0 33.0 40.0 27.0 40.0 66-67 34.185606468470155 37.0 33.0 38.5 27.0 40.0 68-69 33.969041139193614 37.0 33.0 37.0 27.0 40.0 70-71 33.739870294726344 37.0 33.0 37.0 24.5 40.0 72-73 33.51586362341676 37.0 33.0 37.0 22.0 40.0 74-75 33.27599431654475 37.0 33.0 37.0 22.0 40.0 76-77 33.04365838680095 37.0 33.0 37.0 22.0 37.0 78-79 32.811053760679115 37.0 33.0 37.0 22.0 37.0 80-81 32.60002304993439 37.0 33.0 37.0 22.0 37.0 82-83 32.39843787301791 35.0 33.0 37.0 22.0 37.0 84-85 32.2285275866554 33.0 33.0 37.0 22.0 37.0 86-87 32.256752066673585 33.0 33.0 37.0 22.0 37.0 88-89 32.274289279969445 33.0 33.0 37.0 22.0 37.0 90-91 32.289717424268616 33.0 33.0 37.0 22.0 37.0 92-93 32.258295753708154 33.0 33.0 37.0 22.0 37.0 94-95 32.29675473388028 33.0 33.0 37.0 22.0 37.0 96-97 32.25897704391177 33.0 33.0 37.0 22.0 37.0 98-99 32.19146032859328 33.0 33.0 37.0 22.0 37.0 100 31.647485499121633 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6030262075483392 1101 2 4.004152290416336 1101 3 2.3157221898191906 1101 4 1.1250374267013328 1101 5 0.5639767520222847 1101 6 0.5526428490822326 1101 7 0.6406672318160958 1101 8 0.5852288726188348 1101 9 0.6487176095246028 1101 10-11 0.6431467130408919 1101 12-13 0.7318251797362052 1101 14-15 0.9865811441338792 1101 16-17 0.9852814396143401 1101 18-19 1.0222510448483462 1101 20-21 0.9592655560864927 1101 22-23 1.0064230469706175 1101 24-25 1.0194138136107185 1101 26-27 0.9566469064116205 1101 28-29 0.8602629233456511 1101 30-31 0.7872418116421969 1101 32-33 0.9784449106328594 1101 34-35 1.0831294772969216 1101 36-37 0.9652185386243204 1101 38-39 0.9416236048209825 1101 40-41 0.9823932020096962 1101 42-43 1.0314630904285522 1101 44-45 1.0903536875236455 1101 46-47 1.1446361550853865 1101 48-49 1.0679007705819004 1101 50-51 1.0528244625067629 1101 52-53 1.0527462811465327 1101 54-55 1.01406698618743 1101 56-57 1.0811921888316363 1101 58-59 0.9969005733658136 1101 60-61 0.9613881416037415 1101 62-63 0.9884909019517067 1101 64-65 0.9468098335587953 1101 66-67 0.9338244601948773 1101 68-69 0.8330743573045325 1101 70-71 0.9410480029787607 1101 72-73 0.8842818550755212 1101 74-75 0.80620964690835 1101 76-77 0.8476733977919366 1101 78-79 0.8858249094147226 1101 80-81 0.8668853002783052 1101 82-83 0.8451081518829682 1101 84-85 0.8578563483294737 1101 86-87 0.920517800230968 1101 88-89 0.846185758859562 1101 90-91 0.8636878555270115 1101 92-93 0.9449126697949701 1101 94-95 0.8449315219201665 1101 96-97 0.8873733808830764 1101 98-99 0.8524015537857892 1101 100 0.9985387240915955 1102 1 0.5128615180428149 1102 2 3.9263232294375 1102 3 2.2288436503400604 1102 4 1.1431413186844352 1102 5 0.45309281257561196 1102 6 0.5016833057033736 1102 7 0.5328426159770672 1102 8 0.5389595731816428 1102 9 0.5539158642223398 1102 10-11 0.6221392235651919 1102 12-13 0.6437782374513716 1102 14-15 0.9317416078321443 1102 16-17 0.8664647899337652 1102 18-19 0.8937267362211188 1102 20-21 0.8362554507045061 1102 22-23 0.8806610491142308 1102 24-25 0.939209106880277 1102 26-27 0.9484562062582285 1102 28-29 0.9474285143079797 1102 30-31 0.9005889470859003 1102 32-33 0.9589222111754907 1102 34-35 1.050220546367676 1102 36-37 1.0107609334141472 1102 38-39 0.8847284655228762 1102 40-41 0.8722213622628345 1102 42-43 0.9271155353285536 1102 44-45 0.9392400447461995 1102 46-47 0.9464576754109331 1102 48-49 0.8947368034115968 1102 50-51 0.864533585863775 1102 52-53 0.9661671306068982 1102 54-55 0.9064232283188716 1102 56-57 0.861081915733358 1102 58-59 0.8851557536352388 1102 60-61 0.8578042293543078 1102 62-63 0.9102261002941319 1102 64-65 0.8224967100146188 1102 66-67 0.8991439619298021 1102 68-69 0.8597220748568404 1102 70-71 0.8496557822658772 1102 72-73 0.8545201635536017 1102 74-75 0.8858405248380876 1102 76-77 0.9310981806161465 1102 78-79 0.781351969203314 1102 80-81 0.9048075337271086 1102 82-83 0.9178115024071047 1102 84-85 0.8685266944216217 1102 86-87 0.8083778163445956 1102 88-89 0.7815855582433926 1102 90-91 0.8288548297466676 1102 92-93 0.9341621759311742 1102 94-95 0.9338866837656568 1102 96-97 0.9091979755199588 1102 98-99 0.8717138767661439 1102 100 0.8727807473320794 1103 1 0.5109044223551322 1103 2 3.925183770158867 1103 3 2.17596973711397 1103 4 1.003092369186497 1103 5 0.43042087958256303 1103 6 0.4259783901952332 1103 7 0.3593951948706504 1103 8 0.5041852028823399 1103 9 0.5281505079108157 1103 10-11 0.5133151978576791 1103 12-13 0.5211583358661613 1103 14-15 0.7792219300308147 1103 16-17 0.7130236708080844 1103 18-19 0.808631114117432 1103 20-21 0.7872985841797586 1103 22-23 0.726262843143175 1103 24-25 0.7625657930957956 1103 26-27 0.741817462837453 1103 28-29 0.7438271929736473 1103 30-31 0.6899914323616301 1103 32-33 0.6609071877397099 1103 34-35 0.864849606364885 1103 36-37 0.8581505299326366 1103 38-39 0.7392867263309313 1103 40-41 0.7431002979152126 1103 42-43 0.8153968998869985 1103 44-45 0.8447384722905511 1103 46-47 0.8551733645245108 1103 48-49 0.7688584222451027 1103 50-51 0.7333892927945911 1103 52-53 0.7265623593019299 1103 54-55 0.7067812769854243 1103 56-57 0.7012586364633151 1103 58-59 0.6697477975549404 1103 60-61 0.6576480430234781 1103 62-63 0.7552372102754035 1103 64-65 0.6839963143217034 1103 66-67 0.6908876302905114 1103 68-69 0.6894956917185127 1103 70-71 0.7910465323360611 1103 72-73 0.740927144770346 1103 74-75 0.636525757018461 1103 76-77 0.6190999811294446 1103 78-79 0.6579197273782 1103 80-81 0.6600766693849351 1103 82-83 0.6473692105714193 1103 84-85 0.6113227530401737 1103 86-87 0.6139353215044494 1103 88-89 0.6985575605748124 1103 90-91 0.6713123572333259 1103 92-93 0.7324427907362754 1103 94-95 0.6804505752765309 1103 96-97 0.598655715958671 1103 98-99 0.6386769078475609 1103 100 0.7380671125794791 1104 1 0.3372287532155518 1104 2 3.4502604079604815 1104 3 1.9731087117962396 1104 4 0.8818988976685844 1104 5 0.25701990106865935 1104 6 0.21663076718694896 1104 7 0.12078807105669398 1104 8 0.19145039026260946 1104 9 0.23481319386372945 1104 10-11 0.310323147295648 1104 12-13 0.2689615660160172 1104 14-15 0.3954287629171347 1104 16-17 0.41673996172344374 1104 18-19 0.4384409883322675 1104 20-21 0.4690609552724325 1104 22-23 0.3614847175137825 1104 24-25 0.3810011355882068 1104 26-27 0.41963571616197726 1104 28-29 0.41833245243244477 1104 30-31 0.3265246280104108 1104 32-33 0.5411267278673577 1104 34-35 0.5944885761676844 1104 36-37 0.4062152668593271 1104 38-39 0.5025024078708569 1104 40-41 0.47244329333214097 1104 42-43 0.4846873360339927 1104 44-45 0.5063894442752712 1104 46-47 0.4213673806836269 1104 48-49 0.44497026868379663 1104 50-51 0.37774933566696234 1104 52-53 0.29866213097086103 1104 54-55 0.34978459632259273 1104 56-57 0.3356410570253985 1104 58-59 0.3750521451022095 1104 60-61 0.35622435232092897 1104 62-63 0.3792372104612838 1104 64-65 0.39852295466528886 1104 66-67 0.41050397053027865 1104 68-69 0.35932637754725505 1104 70-71 0.3842213392974614 1104 72-73 0.3343140341785329 1104 74-75 0.2854329962573061 1104 76-77 0.3307493405672659 1104 78-79 0.32533793616482853 1104 80-81 0.37748336728203213 1104 82-83 0.39966949477932445 1104 84-85 0.413996307001522 1104 86-87 0.4231631931553821 1104 88-89 0.3877842695190168 1104 90-91 0.30663237855053893 1104 92-93 0.42571179924857105 1104 94-95 0.4428670934448249 1104 96-97 0.396146186364156 1104 98-99 0.3745651242377832 1104 100 0.29825410110121453 1105 1 0.4047616398454963 1105 2 3.6373463719466343 1105 3 2.089531835480134 1105 4 0.9071486268452134 1105 5 0.29779439601045254 1105 6 0.2596004798812075 1105 7 0.2137195952706179 1105 8 0.22866724675046157 1105 9 0.276662514648649 1105 10-11 0.2237683344056336 1105 12-13 0.3366832313612349 1105 14-15 0.4259559805483377 1105 16-17 0.4752498082041683 1105 18-19 0.4280598665499866 1105 20-21 0.33199235691644446 1105 22-23 0.3239466763980303 1105 24-25 0.29464455146005974 1105 26-27 0.3574848298879516 1105 28-29 0.350443130629543 1105 30-31 0.3867338922746981 1105 32-33 0.4682096654994865 1105 34-35 0.5197698764529335 1105 36-37 0.4205508237917357 1105 38-39 0.39104697953018785 1105 40-41 0.42307398102045113 1105 42-43 0.3993292990313577 1105 44-45 0.4808813358546047 1105 46-47 0.4836836193817007 1105 48-49 0.40044630402060477 1105 50-51 0.3394117831841186 1105 52-53 0.23870062634379963 1105 54-55 0.3324281004260641 1105 56-57 0.23798508260302498 1105 58-59 0.3252440605779938 1105 60-61 0.2758158006088749 1105 62-63 0.4879514352515102 1105 64-65 0.31412277578807135 1105 66-67 0.3358826951819296 1105 68-69 0.18782804318814073 1105 70-71 0.2529749052865782 1105 72-73 0.32224245344397673 1105 74-75 0.25566391095258467 1105 76-77 0.3213996422483092 1105 78-79 0.31149813241890456 1105 80-81 0.41851033518874203 1105 82-83 0.3272968860855414 1105 84-85 0.3512459392450076 1105 86-87 0.35177002097309895 1105 88-89 0.2981050466721342 1105 90-91 0.3280174363240107 1105 92-93 0.35910127459134245 1105 94-95 0.3209908250567608 1105 96-97 0.31883561254797854 1105 98-99 0.3592291021010894 1105 100 0.25722045500564406 1106 1 0.47390653350310075 1106 2 3.873613657370665 1106 3 2.13288203199377 1106 4 0.9716128016390115 1106 5 0.3301326969752125 1106 6 0.32079661797070713 1106 7 0.21638841969773637 1106 8 0.23285290473054232 1106 9 0.21951926757939333 1106 10-11 0.21695448146860485 1106 12-13 0.22356653462544074 1106 14-15 0.2747280761679889 1106 16-17 0.28793219478781396 1106 18-19 0.24046565530066033 1106 20-21 0.2669439843288828 1106 22-23 0.19590072892759025 1106 24-25 0.2380933338132536 1106 26-27 0.22807537964233404 1106 28-29 0.2159598855881697 1106 30-31 0.16377736346755256 1106 32-33 0.23811582358790417 1106 34-35 0.38111484097519366 1106 36-37 0.25876116873373434 1106 38-39 0.22807255892705314 1106 40-41 0.2407924755830706 1106 42-43 0.28620696027903136 1106 44-45 0.27746159262450476 1106 46-47 0.2501674484123342 1106 48-49 0.20594782854676907 1106 50-51 0.2266277800596228 1106 52-53 0.1577378744966964 1106 54-55 0.23214818457761055 1106 56-57 0.2699799851859126 1106 58-59 0.184076520445295 1106 60-61 0.20352683241714686 1106 62-63 0.21890037284705244 1106 64-65 0.20736689080568027 1106 66-67 0.11804667289673176 1106 68-69 0.16913045833267404 1106 70-71 0.19137050300296465 1106 72-73 0.17222214913562084 1106 74-75 0.12858962994707213 1106 76-77 0.1537766981898585 1106 78-79 0.11423370178572156 1106 80-81 0.08881342246310453 1106 82-83 0.08281685201193056 1106 84-85 0.2022558300823043 1106 86-87 0.2037671256802227 1106 88-89 0.23433958986389314 1106 90-91 0.2596664798180015 1106 92-93 0.16460722468282185 1106 94-95 0.17388869450156363 1106 96-97 0.2272244407713444 1106 98-99 0.20325494110023357 1106 100 0.14261244702164788 1107 1 0.46416919347834096 1107 2 3.5238326913123252 1107 3 2.1100310397959596 1107 4 0.979049215324828 1107 5 0.41021922602337924 1107 6 0.20574850513776255 1107 7 0.20094389420518866 1107 8 0.17718984091420253 1107 9 0.19957418203866695 1107 10-11 0.19004034405651282 1107 12-13 0.19831602894944922 1107 14-15 0.24789202480798167 1107 16-17 0.2258579115824375 1107 18-19 0.18294145894501668 1107 20-21 0.2984349019857646 1107 22-23 0.29044816276406493 1107 24-25 0.250389951192183 1107 26-27 0.19316954995179003 1107 28-29 0.2229409255810353 1107 30-31 0.17685067817730982 1107 32-33 0.2448485065539927 1107 34-35 0.4469931836990284 1107 36-37 0.19792770274974458 1107 38-39 0.17308587938598663 1107 40-41 0.22344109243023524 1107 42-43 0.2540448127726549 1107 44-45 0.34406696593846675 1107 46-47 0.25868717779331973 1107 48-49 0.24165334057541088 1107 50-51 0.24385410424851273 1107 52-53 0.209110252022235 1107 54-55 0.19449150082087385 1107 56-57 0.25403843077668853 1107 58-59 0.2907772389745915 1107 60-61 0.33734657095249077 1107 62-63 0.34402728892028733 1107 64-65 0.22414553795550063 1107 66-67 0.26682632760851277 1107 68-69 0.22047471974835986 1107 70-71 0.29833933330399276 1107 72-73 0.2679225067384863 1107 74-75 0.30545800142257207 1107 76-77 0.30867247558397537 1107 78-79 0.2959505803727609 1107 80-81 0.3184261350030013 1107 82-83 0.30770899681864705 1107 84-85 0.36412472924505934 1107 86-87 0.4182406249930537 1107 88-89 0.32429258779804115 1107 90-91 0.3202405685662484 1107 92-93 0.37322016520692713 1107 94-95 0.3616625131672393 1107 96-97 0.21362546792479264 1107 98-99 0.26872933777294605 1107 100 0.3044151666226327 1108 1 0.4825392952632903 1108 2 3.584550967133641 1108 3 2.142365945634843 1108 4 0.9349419309737925 1108 5 0.2917889603275796 1108 6 0.15143848342775357 1108 7 0.11464544185106007 1108 8 0.013327706742970236 1108 9 -0.012216966402711193 1108 10-11 0.05317319894295025 1108 12-13 0.10066197900536622 1108 14-15 -0.010519796987139785 1108 16-17 -0.09352051499215008 1108 18-19 0.06067090299065825 1108 20-21 0.06262462498376209 1108 22-23 -0.024256178830015074 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0.9799940161666498 2202 72-73 0.9444211281760602 2202 74-75 0.8316035001018065 2202 76-77 0.8812009392432998 2202 78-79 0.9107011642644807 2202 80-81 0.9089445152686508 2202 82-83 0.9208939009652113 2202 84-85 0.9850713647708282 2202 86-87 0.9568551612623324 2202 88-89 0.9305586384461861 2202 90-91 0.9069377641861323 2202 92-93 1.0086892673905332 2202 94-95 0.936244133921484 2202 96-97 0.966916067522952 2202 98-99 0.9350791900576851 2202 100 0.8749218860520358 2203 1 0.40310832348423986 2203 2 3.8747807934266696 2203 3 2.1580372902303324 2203 4 0.9104262165063162 2203 5 0.5459733247883136 2203 6 0.47015795342707634 2203 7 0.41641486264139616 2203 8 0.42142426358973495 2203 9 0.5185262702621571 2203 10-11 0.5328727907159561 2203 12-13 0.5871381650824787 2203 14-15 0.8605632559955865 2203 16-17 0.8379588915196194 2203 18-19 0.8864098447053337 2203 20-21 0.9136919141077513 2203 22-23 0.847547281919141 2203 24-25 0.872820009324812 2203 26-27 0.8379478030136767 2203 28-29 0.8334370818329191 2203 30-31 0.8442290155348715 2203 32-33 0.8631678167643031 2203 34-35 1.108813185537386 2203 36-37 0.9555316767525923 2203 38-39 0.8245354734888721 2203 40-41 0.47335509291000477 2203 42-43 0.620725536190939 2203 44-45 0.8955066732351895 2203 46-47 0.9128812237389425 2203 48-49 0.8155154835238321 2203 50-51 0.8566349422281974 2203 52-53 0.8458698793277577 2203 54-55 0.8168615094970448 2203 56-57 0.832944089123643 2203 58-59 0.8588167705058751 2203 60-61 0.7704326780842194 2203 62-63 0.8984276238610676 2203 64-65 0.8012691275162851 2203 66-67 0.8385970187209253 2203 68-69 0.8871907083505093 2203 70-71 0.9039122300036837 2203 72-73 0.8090302213617662 2203 74-75 0.7835619541273147 2203 76-77 0.8015440771344728 2203 78-79 0.7346410157285135 2203 80-81 0.7067151906897067 2203 82-83 0.7909361758953821 2203 84-85 0.7543201562048729 2203 86-87 0.8200632386520965 2203 88-89 0.7875601436046935 2203 90-91 0.7901566876473396 2203 92-93 0.8755354601633165 2203 94-95 0.8187979848306597 2203 96-97 0.8145524737021859 2203 98-99 0.8102304284602937 2203 100 0.7749739617325915 2204 1 0.4197138434729304 2204 2 3.899577774403557 2204 3 2.1837334372249337 2204 4 1.0304451329172224 2204 5 0.5898951039528697 2204 6 0.49151421270548923 2204 7 0.45773324843274565 2204 8 0.49314334525182346 2204 9 0.3976323438727931 2204 10-11 0.45421778748970354 2204 12-13 0.4745549880819624 2204 14-15 0.6150003203857324 2204 16-17 0.6142151138545486 2204 18-19 0.6071523947220285 2204 20-21 0.6131740223284012 2204 22-23 0.5516806763858924 2204 24-25 0.638402389551743 2204 26-27 0.5514806814384698 2204 28-29 0.6225053977640371 2204 30-31 0.637480989500979 2204 32-33 0.7986836346161823 2204 34-35 0.7753616504199883 2204 36-37 0.54851018357963 2204 38-39 0.482543640307604 2204 40-41 0.496556360852324 2204 42-43 0.6161963340810459 2204 44-45 0.7231521838673416 2204 46-47 0.6996692802859101 2204 48-49 0.5140070836313697 2204 50-51 0.4483764852496819 2204 52-53 0.5326111686334727 2204 54-55 0.5209809694873542 2204 56-57 0.5202111866709842 2204 58-59 0.6855851384443028 2204 60-61 0.6536396758478915 2204 62-63 0.725013373910727 2204 64-65 0.6424382851207255 2204 66-67 0.5370749385097895 2204 68-69 0.590358469015392 2204 70-71 0.4970989473027103 2204 72-73 0.49306612267287164 2204 74-75 0.4967748479094354 2204 76-77 0.5301689107274257 2204 78-79 0.5802218440459512 2204 80-81 0.5489752045282614 2204 82-83 0.4800191755158707 2204 84-85 0.7254356772358328 2204 86-87 0.6715374443780959 2204 88-89 0.6043374256299217 2204 90-91 0.60286555561445 2204 92-93 0.7986167855249668 2204 94-95 0.6088231716454757 2204 96-97 0.6340480063156662 2204 98-99 0.7759226203178571 2204 100 0.5089931173612214 2205 1 0.3931300285104058 2205 2 3.6873588517293854 2205 3 2.0757359121486587 2205 4 1.0173503727441329 2205 5 0.44366059277169967 2205 6 0.2851352499173814 2205 7 0.30970727704432477 2205 8 0.32144231477662544 2205 9 0.1912509268753908 2205 10-11 0.3357711398607961 2205 12-13 0.373312460192345 2205 14-15 0.5520595337013248 2205 16-17 0.6004761687727722 2205 18-19 0.629834242061655 2205 20-21 0.6038519897657793 2205 22-23 0.5749467381162319 2205 24-25 0.5880164707689559 2205 26-27 0.5542245887513175 2205 28-29 0.5873584187718421 2205 30-31 0.5286750839078778 2205 32-33 0.6255409025318528 2205 34-35 0.7040461897345054 2205 36-37 0.6229594218628662 2205 38-39 0.5030192435533749 2205 40-41 0.5557321202893846 2205 42-43 0.6080765375957853 2205 44-45 0.6855111991124829 2205 46-47 0.6703087639202749 2205 48-49 0.5961975960184844 2205 50-51 0.4581289613554844 2205 52-53 0.4622283750273013 2205 54-55 0.5028511287565323 2205 56-57 0.4154996226844574 2205 58-59 0.5380165055807282 2205 60-61 0.40944545692926937 2205 62-63 0.5430580715915454 2205 64-65 0.5133530454784392 2205 66-67 0.44890448624405366 2205 68-69 0.45772968888574894 2205 70-71 0.516389764124348 2205 72-73 0.5177841531631628 2205 74-75 0.37271685336768456 2205 76-77 0.5178501197474787 2205 78-79 0.4928001958034969 2205 80-81 0.43290404011403183 2205 82-83 0.45010269068210107 2205 84-85 0.4451362006578812 2205 86-87 0.4804417772733487 2205 88-89 0.4648015211108145 2205 90-91 0.42508414280496964 2205 92-93 0.5520522394827587 2205 94-95 0.505797075295014 2205 96-97 0.463508469791968 2205 98-99 0.4973223909731246 2205 100 0.4293680304788339 2206 1 0.3758379983288158 2206 2 3.571039980874353 2206 3 2.112007310284003 2206 4 0.9301531241325875 2206 5 0.49110379353025735 2206 6 0.3914229160118552 2206 7 0.314041732994788 2206 8 0.1655945480349601 2206 9 0.31408443406734676 2206 10-11 0.26203003229719 2206 12-13 0.3049978966313631 2206 14-15 0.4162146672222775 2206 16-17 0.4831402193666392 2206 18-19 0.3097744364914874 2206 20-21 0.24607415661662202 2206 22-23 0.39701569388027025 2206 24-25 0.2490823868692118 2206 26-27 0.3410760169622975 2206 28-29 0.26893248018782145 2206 30-31 0.4160625107306899 2206 32-33 0.2859678068225122 2206 34-35 0.39757482163438596 2206 36-37 0.20829458738996465 2206 38-39 0.3604575753725996 2206 40-41 0.45147706239524865 2206 42-43 0.41305079103070597 2206 44-45 0.52663870763201 2206 46-47 0.40654509850911325 2206 48-49 0.35104060808038184 2206 50-51 0.25326117808771187 2206 52-53 0.13788508135707644 2206 54-55 0.11571914727780097 2206 56-57 0.19359756670804273 2206 58-59 0.20110662316033512 2206 60-61 0.27484841048309505 2206 62-63 0.32149304768121567 2206 64-65 0.05725691569033842 2206 66-67 0.2131515773951307 2206 68-69 -0.05320674799795455 2206 70-71 0.09195771164306876 2206 72-73 0.13084200195397955 2206 74-75 0.12344710447610652 2206 76-77 0.127596562267847 2206 78-79 0.15131111971447098 2206 80-81 0.18485931546051404 2206 82-83 0.2516851184129223 2206 84-85 0.215834254810634 2206 86-87 0.24643695008702338 2206 88-89 0.26937582114747727 2206 90-91 0.21394284681408493 2206 92-93 0.265088695469597 2206 94-95 0.2488075097305611 2206 96-97 0.3277793444433783 2206 98-99 0.23497138017997088 2206 100 -0.06292488078098657 2207 1 0.3203378912579673 2207 2 3.740704200336932 2207 3 2.0737937919540173 2207 4 0.9711935397489668 2207 5 0.42383045253863116 2207 6 0.3812685162165579 2207 7 0.31535879518843757 2207 8 0.14359119345078142 2207 9 0.09477717793028262 2207 10-11 0.18783883687837033 2207 12-13 0.2446718545507025 2207 14-15 0.33593407807853737 2207 16-17 0.2916297922083899 2207 18-19 0.30963253358842024 2207 20-21 0.3314820330387178 2207 22-23 0.26591081798263616 2207 24-25 0.2773473786029754 2207 26-27 0.24537821218693523 2207 28-29 0.29403564930350257 2207 30-31 0.25139708218414825 2207 32-33 0.35326700814656675 2207 34-35 0.4614937145580953 2207 36-37 0.29534363082987625 2207 38-39 0.19447799959094425 2207 40-41 0.21546516573494756 2207 42-43 0.3159941618932791 2207 44-45 0.3902778439332053 2207 46-47 0.3164495491176993 2207 48-49 0.21540299436297516 2207 50-51 0.12307316439715521 2207 52-53 0.2114190273534433 2207 54-55 0.2113371593169262 2207 56-57 0.21203991949015943 2207 58-59 0.33666186945051635 2207 60-61 0.23910504161676016 2207 62-63 0.3027212025056656 2207 64-65 0.19246161836911568 2207 66-67 0.16176943336487426 2207 68-69 -0.017997848686299278 2207 70-71 0.002032035145845157 2207 72-73 0.18793491268409923 2207 74-75 0.19939547545663316 2207 76-77 0.2858109720987656 2207 78-79 0.2215297937781031 2207 80-81 0.11572088873055009 2207 82-83 0.2842277153710171 2207 84-85 -0.15693721970015062 2207 86-87 0.03307294276991257 2207 88-89 0.2099924562373161 2207 90-91 0.3904844175253075 2207 92-93 0.3744642795955251 2207 94-95 0.33799468091870466 2207 96-97 0.3211024447978019 2207 98-99 0.32929701076376006 2207 100 0.019096766585704472 2208 1 0.44455185315709045 2208 2 3.6654236593593588 2208 3 2.134880710027751 2208 4 1.006517610146794 2208 5 0.43961871042337464 2208 6 0.30473433876378664 2208 7 0.2948050403247606 2208 8 0.2794675027623228 2208 9 0.22984137119224357 2208 10-11 0.2855781313841419 2208 12-13 0.32823006883383954 2208 14-15 0.33789903797804755 2208 16-17 0.3227475075274242 2208 18-19 0.31249122720049627 2208 20-21 0.3316054746782555 2208 22-23 0.013725413693229882 2208 24-25 0.2989091048368451 2208 26-27 0.21854206291639855 2208 28-29 0.33354401229411934 2208 30-31 0.3531502151474015 2208 32-33 0.5014512971299467 2208 34-35 0.55644145037855 2208 36-37 0.29130971505894365 2208 38-39 0.21795275541472137 2208 40-41 0.39771691088210304 2208 42-43 0.20665884278580648 2208 44-45 -0.08827096785078936 2208 46-47 0.014517750623539882 2208 48-49 0.15736940704786662 2208 50-51 0.26934997325029997 2208 52-53 0.3559527644588414 2208 54-55 0.2748613278905623 2208 56-57 0.2719254923387737 2208 58-59 0.22001209178984027 2208 60-61 0.31447829917665615 2208 62-63 0.3760366482310431 2208 64-65 0.32277389503719434 2208 66-67 0.2529860373482151 2208 68-69 0.3833004992891844 2208 70-71 0.3424909348549292 2208 72-73 0.2992941152222386 2208 74-75 0.297053423004769 2208 76-77 0.31043302231280734 2208 78-79 0.17532607536941214 2208 80-81 0.14599941746502054 2208 82-83 0.26203041809323224 2208 84-85 0.2925275055880583 2208 86-87 0.3402402278479144 2208 88-89 0.30190951031341484 2208 90-91 0.3025787204409838 2208 92-93 0.3341260693549195 2208 94-95 0.28415865075852054 2208 96-97 0.2448882861861179 2208 98-99 0.32386007428205943 2208 100 -0.14829361579462486 2209 1 0.3970264892252544 2209 2 3.459892966575069 2209 3 2.059191203416983 2209 4 0.8919088768397572 2209 5 0.4456344577390894 2209 6 0.28993659045411846 2209 7 0.1863561630781092 2209 8 0.23576938873707576 2209 9 0.16433002914696715 2209 10-11 0.13513873184193415 2209 12-13 0.13525636991294476 2209 14-15 0.0957519735947372 2209 16-17 0.16219556751190112 2209 18-19 0.17722748705710245 2209 20-21 0.2214715116824877 2209 22-23 0.13651447636802772 2209 24-25 0.22728965727367267 2209 26-27 0.13824625153043257 2209 28-29 0.13377546714588817 2209 30-31 0.09465900815914807 2209 32-33 0.14237350765237977 2209 34-35 0.3440164842608411 2209 36-37 0.21019413103711315 2209 38-39 0.139858566256315 2209 40-41 0.07689664584533062 2209 42-43 0.146242374679737 2209 44-45 0.21554050144357007 2209 46-47 0.074625807517954 2209 48-49 -0.09916551932718676 2209 50-51 -0.04048933316734349 2209 52-53 0.06426856133175107 2209 54-55 0.08216824903894349 2209 56-57 0.015345179636895523 2209 58-59 0.010009029038592132 2209 60-61 -0.4350549990162236 2209 62-63 -0.07995409586512636 2209 64-65 0.008044008204841191 2209 66-67 0.05372462549475898 2209 68-69 0.06805110307487894 2209 70-71 0.11711822524585358 2209 72-73 0.053482275614214814 2209 74-75 0.03331786008482851 2209 76-77 0.061677721956172604 2209 78-79 0.0362089898331277 2209 80-81 0.07946354680773027 2209 82-83 -0.14790271756330498 2209 84-85 -0.14038778172478672 2209 86-87 0.010530981334049727 2209 88-89 0.08872255599536061 2209 90-91 0.05082505971771667 2209 92-93 0.16943774151139834 2209 94-95 0.10003643068755252 2209 96-97 0.033521475522285016 2209 98-99 -0.03259455727257077 2209 100 -0.08032132625020694 2210 1 0.5196514694783048 2210 2 3.779723830086187 2210 3 2.2301031092820907 2210 4 1.0725561514148723 2210 5 0.5034277252136121 2210 6 0.3850477904932461 2210 7 0.32081101169308823 2210 8 0.3277343581035197 2210 9 0.2986264162349457 2210 10-11 0.34613549210006767 2210 12-13 0.3231669863817359 2210 14-15 0.2774508982051671 2210 16-17 0.24969896755096954 2210 18-19 0.30345542891559774 2210 20-21 0.33132916302026416 2210 22-23 0.2533132554961952 2210 24-25 0.3437945942005811 2210 26-27 0.31373016387912855 2210 28-29 0.2369353789602897 2210 30-31 0.2157293377580416 2210 32-33 0.3884339705241686 2210 34-35 0.47528531135291274 2210 36-37 0.33100141990022536 2210 38-39 0.2815977050009053 2210 40-41 0.2904340275944364 2210 42-43 0.32115377389988 2210 44-45 0.4546242029277465 2210 46-47 0.3749983513777835 2210 48-49 0.2834808972340994 2210 50-51 0.27528729082574444 2210 52-53 0.30817081599902707 2210 54-55 0.26142338375931473 2210 56-57 0.26668489491149217 2210 58-59 0.25277856723688075 2210 60-61 0.2711413487992118 2210 62-63 0.342618092673753 2210 64-65 -0.0222062433089647 2210 66-67 0.12532226754404263 2210 68-69 0.1835725257235694 2210 70-71 0.24997182957881137 2210 72-73 0.30929697680544166 2210 74-75 -0.22066594908840642 2210 76-77 0.017219815330676624 2210 78-79 0.24530102633963935 2210 80-81 0.3624998057205886 2210 82-83 0.29202065724501836 2210 84-85 0.3601953944877785 2210 86-87 0.24602403100091408 2210 88-89 0.35870979160891636 2210 90-91 0.35935540275155375 2210 92-93 0.3636239203620413 2210 94-95 0.2983006587779897 2210 96-97 0.20873675913926348 2210 98-99 0.19019187557002937 2210 100 0.09992490838536838 2211 1 0.47260480821937634 2211 2 3.947579661364717 2211 3 2.2834554369642 2211 4 1.0845096771399696 2211 5 0.5629955903268353 2211 6 0.35239351858080425 2211 7 0.20794799027332544 2211 8 0.36630284066759344 2211 9 0.29705386710455883 2211 10-11 0.24199009654813608 2211 12-13 0.2631030755098891 2211 14-15 0.32929322896781343 2211 16-17 0.22648368974516586 2211 18-19 0.37447270274227407 2211 20-21 0.3626153575455362 2211 22-23 0.324663804812225 2211 24-25 0.2606526168243093 2211 26-27 0.26296366388383774 2211 28-29 0.31896755904608654 2211 30-31 0.21985139393665065 2211 32-33 0.3054641798790243 2211 34-35 0.46070358386263166 2211 36-37 0.20661702109423175 2211 38-39 0.19040196231782858 2211 40-41 0.15979877962535483 2211 42-43 0.278069425270175 2211 44-45 0.2869977297559245 2211 46-47 0.3480521368627123 2211 48-49 0.18379520527885518 2211 50-51 0.25108261192036707 2211 52-53 0.24669309063956035 2211 54-55 0.36154828639982384 2211 56-57 0.26162590077794334 2211 58-59 0.2885960977294033 2211 60-61 0.26998850482595316 2211 62-63 0.34153788997768686 2211 64-65 0.39131263467924526 2211 66-67 0.4296189318296868 2211 68-69 0.330954278503782 2211 70-71 0.24423081456493634 2211 72-73 0.34642440440293853 2211 74-75 0.36215317816701287 2211 76-77 0.26782415040067065 2211 78-79 0.2327155887633836 2211 80-81 0.29539708960942335 2211 82-83 0.02225785791922874 2211 84-85 0.12208704060638453 2211 86-87 0.25471878831851313 2211 88-89 0.25556489512946 2211 90-91 0.3566965974853318 2211 92-93 0.47985164538521374 2211 94-95 0.3769250885776003 2211 96-97 0.32479835087107745 2211 98-99 0.41076913158614303 2211 100 0.262540478639572 2212 1 0.7423648101222362 2212 2 4.23804812147603 2212 3 2.480014829952168 2212 4 1.2645617679063506 2212 5 0.7900007280462589 2212 6 0.7358761074205376 2212 7 0.6008556778238727 2212 8 0.6406802489735739 2212 9 0.3577848028354964 2212 10-11 0.4537337100774579 2212 12-13 0.44768337616162057 2212 14-15 0.6599209345955117 2212 16-17 0.6661853571254 2212 18-19 0.7143486006538851 2212 20-21 0.6767075153876903 2212 22-23 0.504651971407533 2212 24-25 0.5752621144695169 2212 26-27 0.5250037071738802 2212 28-29 0.6386344992487452 2212 30-31 0.6077655631008199 2212 32-33 0.7923062068996174 2212 34-35 0.7954224364386207 2212 36-37 0.7355776438048522 2212 38-39 0.6623243851331111 2212 40-41 0.6727367345275965 2212 42-43 0.6331696821561508 2212 44-45 0.7568061951001113 2212 46-47 0.9264677688497684 2212 48-49 0.7959568531547916 2212 50-51 0.6457593637399839 2212 52-53 0.6432340496805153 2212 54-55 0.5385498562585411 2212 56-57 0.7041417182937693 2212 58-59 0.686106325596775 2212 60-61 0.6781710960442595 2212 62-63 0.6541508722335223 2212 64-65 0.8319984369721922 2212 66-67 0.7450683633505477 2212 68-69 0.8252308352803368 2212 70-71 0.7931580777779104 2212 72-73 0.8114668024810499 2212 74-75 0.7842667788520359 2212 76-77 0.7659019654427723 2212 78-79 0.8040936154985516 2212 80-81 0.7593334508672172 2212 82-83 0.8804205285819009 2212 84-85 0.8803763703242815 2212 86-87 0.7819371057154001 2212 88-89 0.8032121883124717 2212 90-91 0.7229181735283348 2212 92-93 0.6830844245108487 2212 94-95 0.7086172059839981 2212 96-97 0.6743282852224368 2212 98-99 0.7702266383650809 2212 100 0.651722724964678 2213 1 -0.7655072355358392 2213 2 -25.858542787614876 2213 3 -14.794361385963592 2213 4 -6.390872971982425 2213 5 -2.3427804309793814 2213 6 -1.5223505364694958 2213 7 -1.4059402911609737 2213 8 -1.173532560497165 2213 9 -1.2437839916779012 2213 10-11 -1.2185713784510313 2213 12-13 -1.242659375225891 2213 14-15 -1.420080707680313 2213 16-17 -1.4869230885236888 2213 18-19 -1.4833239470914208 2213 20-21 -1.5699539799816478 2213 22-23 -1.4936356577637824 2213 24-25 -1.3485704134000471 2213 26-27 -1.5989715372725612 2213 28-29 -1.4647319381075548 2213 30-31 -1.5205925926936104 2213 32-33 -1.4868376094405988 2213 34-35 -1.496512094305622 2213 36-37 -1.5750975175452382 2213 38-39 -1.5532391435702237 2213 40-41 -1.5617359510565834 2213 42-43 -1.612215978951113 2213 44-45 -1.4622862966113175 2213 46-47 -1.508954646952013 2213 48-49 -1.706038441795144 2213 50-51 -1.6973576757841258 2213 52-53 -1.7709407207517884 2213 54-55 -1.698665658054523 2213 56-57 -1.5723834927456934 2213 58-59 -1.7349403866231583 2213 60-61 -1.7078490835954554 2213 62-63 -1.5399378822476422 2213 64-65 -1.60583765056532 2213 66-67 -1.429158328263739 2213 68-69 -1.7426307742061198 2213 70-71 -1.5659192158638149 2213 72-73 -1.5235354468753677 2213 74-75 -1.6006461805290044 2213 76-77 -1.5045551592270172 2213 78-79 -1.5083751531876004 2213 80-81 -1.5197457973634805 2213 82-83 -1.5123197349682762 2213 84-85 -1.4512285963669953 2213 86-87 -1.4100818723960096 2213 88-89 -1.5409845247496605 2213 90-91 -1.4245760048143943 2213 92-93 -1.4342643422382615 2213 94-95 -1.5419526777653303 2213 96-97 -1.5957544145948077 2213 98-99 -1.5646801624816078 2213 100 -1.6282670564301647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 34.0 5 51.0 6 27.0 7 39.0 8 55.0 9 110.0 10 407.0 11 1898.0 12 5531.0 13 10012.0 14 13332.0 15 15103.0 16 16187.0 17 17076.0 18 17722.0 19 18565.0 20 20028.0 21 22763.0 22 24898.0 23 26902.0 24 28449.0 25 30268.0 26 34042.0 27 38402.0 28 45569.0 29 54366.0 30 65806.0 31 81046.0 32 103082.0 33 137531.0 34 188081.0 35 267830.0 36 407759.0 37 685426.0 38 654673.0 39 3803.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.09721250758129 20.69033169795399 10.648932112447794 30.563523682016925 2 12.857544123278878 22.36031982845451 36.44435448058777 28.337781567678842 3 15.184210136373292 27.13214362743403 28.720580463204897 28.963065772987783 4 10.615056072104231 18.71993255870241 37.408548382051 33.256462987142356 5 11.769148322202986 38.534553549344544 35.221337290790665 14.474960837661802 6 29.876394828858054 39.57546531085463 17.783238101639117 12.764901758648195 7 26.186633181984682 33.01441852865918 23.211057587152844 17.587890702203296 8 24.61981307228987 36.8207465249182 20.735028060239582 17.824412342552343 9 24.90287998360827 16.828140806811792 21.673452083340884 36.595527126239055 10-11 23.223072692566856 27.600880275513518 29.005377942480344 20.17066908943928 12-13 24.066911432989823 24.964303767404022 29.258985905814654 21.7097988937915 14-15 21.66251319749046 26.42593016571601 26.251896238488744 25.65966039830479 16-17 21.091210455730298 29.952710667118374 27.19137464176432 21.764704235387004 18-19 21.17587131649867 29.038404657649213 28.983250539024873 20.80247348682724 20-21 22.225116931049463 28.284864160390438 28.228411848925184 21.26160705963492 22-23 21.47046840628169 28.25440050010062 28.361244968610322 21.91388612500737 24-25 21.60048229896278 28.154481899132854 28.22334881603904 22.021686985865323 26-27 21.54130311178118 28.09675981476752 28.27368999625721 22.088247077194087 28-29 21.34946824513732 27.99999802392012 28.724593582120693 21.92594014882187 30-31 21.9399323982165 28.39815062599581 28.052978060298155 21.608938915489535 32-33 21.064609047061218 28.34720602072712 28.66447634343895 21.923708588772715 34-35 21.22110651139028 27.97087439864317 28.681660516234558 22.126358573732 36-37 21.824817482193595 28.26719674942871 28.130718988684812 21.777266779692887 38-39 21.58730263271635 28.327695346170216 28.324089449293925 21.760912571819503 40-41 21.69379198153245 28.120421085215668 28.19937250498283 21.986414428269054 42-43 21.474911041374508 28.1883461277991 28.36671617822779 21.970026652598598 44-45 21.85123189112148 27.789472020317245 28.28403309982902 22.075262988732252 46-47 21.754903604248334 28.258862747823155 28.26246900512418 21.72376464280433 48-49 21.67292271294752 28.15953667325455 28.29049287917396 21.877047734623968 50-51 21.7883446554585 28.07801957554052 28.03508815843817 22.098547610562807 52-53 21.43106644060595 28.272537657801276 27.964687757066525 22.33170814452625 54-55 21.724254002821315 28.26448180948965 27.79886244878829 22.21240173890074 56-57 21.83051904682237 27.979602241066097 27.870707268974037 22.319171443137492 58-59 21.456341214808766 27.757986724607232 28.402012880193777 22.383659180390225 60-61 21.942768733439113 28.10993959536601 27.813152679282418 22.134138991912458 62-63 21.37421449568239 28.280576107033934 28.13518751406913 22.21002188321454 64-65 21.399044130929987 27.86610455606332 28.222542312677508 22.512309000329182 66-67 21.69026548818297 28.024014297784998 27.96652608319834 22.31919413083369 68-69 21.826773401562157 27.999712126711678 27.95080001765447 22.222714454071692 70-71 21.80986571574108 27.87616645852955 27.808776474901105 22.50519135082827 72-73 21.640131817627143 28.081268323900105 28.088942479895273 22.18965737857748 74-75 21.937007350141563 27.732934536443075 27.832781973906272 22.497276139509086 76-77 21.71307137234754 28.05586019964892 28.01195851570811 22.219109912295433 78-79 21.779335521749545 27.997846163726138 28.075848628781873 22.146969685742445 80-81 21.80317613794759 27.85163049465229 27.920492977656906 22.42470038974321 82-83 21.373233082310602 28.05221817242983 28.06894716667479 22.50560157858478 84-85 21.50800931119578 27.78914755334121 27.814850985136303 22.88799215032671 86-87 21.70502428769887 27.908550939622167 27.81693590520643 22.56948886747253 88-89 21.460560249760963 27.660687461748722 28.16000192316337 22.718750365326944 90-91 21.688950784489972 27.670615804333355 27.957987976570926 22.682445434605746 92-93 21.177365889190508 28.048053022116754 28.059973580486243 22.714607508206498 94-95 21.134849018446612 27.61532571061837 28.294155331298654 22.955669939636355 96-97 21.47670509841512 27.772641800046134 28.02239026519372 22.728262836345028 98-99 21.306921270913804 27.643577104057798 28.074629470474534 22.974872154553864 100 21.36284552250565 27.554148643490194 28.007109718703294 23.07589611530086 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13.0 1 12.0 2 9.0 3 10.5 4 16.0 5 22.5 6 38.5 7 80.5 8 134.5 9 160.0 10 152.0 11 147.5 12 179.0 13 220.5 14 247.0 15 305.5 16 398.0 17 485.5 18 588.5 19 749.5 20 1005.5 21 1361.0 22 1907.5 23 2938.5 24 4558.0 25 6886.5 26 10565.5 27 16109.5 28 23758.5 29 33048.0 30 42988.0 31 53950.5 32 65438.0 33 76411.0 34 86139.5 35 95670.5 36 104200.5 37 111480.5 38 117885.0 39 121969.5 40 124926.0 41 125507.0 42 123538.0 43 121219.0 44 117258.0 45 112931.5 46 109546.0 47 105775.5 48 101420.5 49 99357.5 50 107917.5 51 103552.5 52 84163.5 53 74690.5 54 69413.5 55 64671.5 56 60134.0 57 54647.0 58 49395.0 59 44785.5 60 39349.5 61 33427.5 62 27860.5 63 22490.5 64 17842.5 65 14455.0 66 11408.5 67 8538.0 68 6408.5 69 4877.0 70 3701.5 71 2756.0 72 2123.5 73 1597.0 74 1107.0 75 717.0 76 467.5 77 284.0 78 172.0 79 100.5 80 46.0 81 24.0 82 15.0 83 8.5 84 5.0 85 5.0 86 5.0 87 3.0 88 2.0 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8079331288474868 2 5.160419311235032 3 0.0 4 0.005597841209001988 5 0.15262349413955417 6 0.054529559071219355 7 0.03520054266131249 8 0.04191795211211488 9 0.039777601061614126 10-11 0.04097949049766455 12-13 0.03953063747886403 14-15 0.04181916667901485 16-17 0.03291201346116168 18-19 0.0278904206119099 20-21 0.022852363523808115 22-23 0.022918220479208135 24-25 0.019559515753806943 26-27 0.0123975718540544 28-29 0.01878569652785667 30-31 0.023790825138258444 32-33 0.001399460302250497 34-35 0.003506882875051245 36-37 0.004165452429051479 38-39 0.006338731957252251 40-41 0.006832659122752425 42-43 0.007260729332852578 44-45 0.008330904858102959 46-47 0.016332524939205798 48-49 0.02487746490235883 50-51 0.021304725071907563 52-53 0.02724831529675967 54-55 0.023510933077808348 56-57 0.01557516995210553 58-59 0.014488530188005142 60-61 0.011953037405104243 62-63 0.017764913719156308 64-65 0.0194442660818569 66-67 0.020102835635857137 68-69 0.027231851057909665 70-71 0.027182458341359652 72-73 0.023724968182858422 74-75 0.0247951437081088 76-77 0.01827530512350649 78-79 0.014570851382255173 80-81 0.013121998363454658 82-83 0.007935763125702816 84-85 0.010718219491353804 86-87 0.008742510829353104 88-89 0.007293657810552589 90-91 0.007590014109852695 92-93 0.003770310696651339 94-95 0.0013336033468504733 96-97 9.713900921503449E-4 98-99 4.939271655001754E-4 100 0.10836762011073847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3036885.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.01841070376295 #Duplication Level Percentage of deduplicated Percentage of total 1 88.17156253712668 71.4351986602545 2 8.63317551873495 13.9889231970908 3 1.7598484765692903 4.277403799532468 4 0.5907549239842612 1.9144810022650858 5 0.2667366751470435 1.0805290748409686 6 0.14566356604116026 0.7080858370858453 7 0.09343366142633898 0.5298892728496827 8 0.06033452820775548 0.3910566068762964 9 0.04346930992030306 0.31696329637190435 >10 0.221028576030388 3.1067883762360124 >50 0.009595454542355943 0.5370255737627655 >100 0.003337161862890758 0.45419982372018675 >500 6.113136964945733E-4 0.32503355041321624 >1k 3.667882174076781E-4 0.5009227943573942 >5k 8.15084927572618E-5 0.43349913434303794 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 7534 0.24808315099188807 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5595 0.1842348327315654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.292847770001169E-5 0.0 4 0.0 0.0 0.0 3.292847770001169E-5 0.0 5 0.0 0.0 0.0 3.292847770001169E-5 0.0 6 0.0 0.0 0.0 3.292847770001169E-5 0.0 7 0.0 0.0 0.0 3.292847770001169E-5 0.0 8 0.0 0.0 0.0 3.292847770001169E-5 0.0 9 0.0 0.0 0.0 6.585695540002338E-5 0.0 10-11 0.0 0.0 0.0 8.232119425002923E-5 0.0 12-13 0.0 0.0 0.0 9.878543310003507E-5 0.0 14-15 0.0 1.6464238850005844E-5 0.0 9.878543310003507E-5 0.0 16-17 0.0 3.292847770001169E-5 0.0 9.878543310003507E-5 0.0 18-19 0.0 3.292847770001169E-5 0.0 9.878543310003507E-5 0.0 20-21 0.0 3.292847770001169E-5 0.0 9.878543310003507E-5 0.0 22-23 0.0 3.292847770001169E-5 0.0 9.878543310003507E-5 0.0 24-25 3.292847770001169E-5 3.292847770001169E-5 0.0 1.3171391080004676E-4 0.0 26-27 3.292847770001169E-5 3.292847770001169E-5 0.0 1.6464238850005846E-4 0.0 28-29 3.292847770001169E-5 3.292847770001169E-5 0.0 6.256410763002221E-4 0.0 30-31 3.292847770001169E-5 3.292847770001169E-5 0.0 0.002469635827500877 0.0 32-33 3.292847770001169E-5 3.292847770001169E-5 0.0 0.007112551183202524 0.0 34-35 3.292847770001169E-5 3.292847770001169E-5 0.0 0.014241566605255055 0.0 36-37 3.292847770001169E-5 3.292847770001169E-5 0.0 0.02583239075565917 0.0 38-39 3.292847770001169E-5 3.292847770001169E-5 0.0 0.04527665683751607 0.0 40-41 3.292847770001169E-5 3.292847770001169E-5 0.0 0.07021997869527494 0.0 42-43 3.292847770001169E-5 6.585695540002338E-5 0.0 0.09453765947673357 0.0 44-45 3.292847770001169E-5 6.585695540002338E-5 0.0 0.12549042851474454 0.0 46-47 3.292847770001169E-5 8.232119425002923E-5 0.0 0.16325939243665796 0.0 48-49 3.292847770001169E-5 9.878543310003507E-5 0.0 0.2039095981573224 0.0 50-51 3.292847770001169E-5 9.878543310003507E-5 0.0 0.247836187409138 0.0 52-53 3.292847770001169E-5 9.878543310003507E-5 0.0 0.2970807258095055 0.0 54-55 3.292847770001169E-5 9.878543310003507E-5 0.0 0.3510175722821246 0.0 56-57 3.292847770001169E-5 9.878543310003507E-5 0.0 0.4119681845048463 0.0 58-59 3.292847770001169E-5 9.878543310003507E-5 0.0 0.48118384463027086 0.0 60-61 3.292847770001169E-5 9.878543310003507E-5 0.0 0.5571663069230478 0.0 62-63 3.292847770001169E-5 9.878543310003507E-5 0.0 0.6400966780105273 0.0 64-65 3.292847770001169E-5 9.878543310003507E-5 0.0 0.7292669956221589 0.0 66-67 3.292847770001169E-5 9.878543310003507E-5 0.0 0.8306043857439449 0.0 68-69 3.292847770001169E-5 9.878543310003507E-5 0.0 0.9473193749516363 0.0 70-71 3.292847770001169E-5 9.878543310003507E-5 0.0 1.0822932050439842 0.0 72-73 3.292847770001169E-5 9.878543310003507E-5 0.0 1.2262894380261353 0.0 74-75 3.292847770001169E-5 1.3171391080004676E-4 0.0 1.3733150909566876 0.0 76-77 3.292847770001169E-5 1.6464238850005846E-4 0.0 1.5367226615429956 0.0 78-79 3.292847770001169E-5 1.6464238850005846E-4 0.0 1.7249418400762624 0.0 80-81 3.292847770001169E-5 1.6464238850005846E-4 0.0 1.9337413171720366 0.0 82-83 3.292847770001169E-5 1.6464238850005846E-4 0.0 2.1593343178948166 0.0 84-85 3.292847770001169E-5 1.6464238850005846E-4 0.0 2.391167265141749 0.0 86-87 3.292847770001169E-5 1.6464238850005846E-4 0.0 2.6411108751236876 0.0 88 3.292847770001169E-5 1.6464238850005846E-4 0.0 2.8493670323374114 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 1665 0.0 15.248279 5 CATGGGG 4750 0.0 14.74803 4 TAGACAG 1710 0.0 14.572064 5 CCTATAC 905 0.0 14.026732 3 GTATATA 2270 0.0 13.987512 1 GTATCAA 11825 0.0 13.971063 1 TATACAG 1790 0.0 13.920799 5 GAGTACT 3160 0.0 13.891308 12-13 ATTATAC 1585 0.0 13.644885 3 CAACGCA 12665 0.0 13.2527 5 AACGCAG 12730 0.0 13.130555 6 TCTATAC 1365 0.0 13.0885725 3 CTAGCAC 1260 0.0 13.05987 3 TCAACGC 12850 0.0 13.025313 4 ATCAACG 13030 0.0 12.953627 3 GTACATG 14785 0.0 12.818238 1 GTCTCGC 465 4.7510912E-7 12.803085 1 GAACCGT 295 3.9176337E-4 12.732963 6 AGTACTT 3850 0.0 12.682101 12-13 GTATAAC 785 1.8189894E-12 12.639989 1 >>END_MODULE