FastQCFastQC Report
Wed 25 May 2016
SRR1295104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479495
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC1900.001312204317.5577533
GTATAGG8150.017.317371
GTACATG81350.017.1230051
GTACCGT2353.2721764E-517.044416
TACATGG84500.016.48182
GTACGGA2250.002102189716.3636591
GTACTAG5751.1459633E-1016.0079271
GTATTAG10550.015.7044581
GTACAGA12700.014.9785461
AACACCG3755.3700387E-714.6792525
GTATATA15500.014.6481131
ACATGGG89250.014.5773593
GTATAAC5902.8194336E-914.5608831
TACACTG16100.014.2979735
TCTATAC8250.014.1526133
ATAGCGT2150.005987883513.9821218
GTATTAA12550.013.690711
GTACTGG10900.013.5112781
TACCGTA2250.00834851813.2972457
TATACAG13550.013.2955315
Produced by FastQC (version 0.11.5)