##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295104_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2479495
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.954109203688656	27.0	22.0	33.0	6.0	33.0
2	22.298280899941318	27.0	6.0	33.0	2.0	33.0
3	24.501084696682188	27.0	15.0	33.0	15.0	33.0
4	24.92107949400987	33.0	15.0	37.0	2.0	37.0
5	26.458646216265812	33.0	15.0	37.0	2.0	37.0
6	27.291442007344237	33.0	15.0	37.0	6.0	37.0
7	27.053817813708033	33.0	15.0	37.0	6.0	37.0
8	28.01296635000272	33.0	22.0	37.0	6.0	37.0
9	28.91261930352753	33.0	22.0	37.0	15.0	37.0
10-11	28.45556655689969	33.0	21.0	37.0	10.5	37.0
12-13	27.227580817868155	33.0	15.0	37.0	4.0	37.0
14-15	28.91828819981488	33.0	22.0	37.0	10.5	40.0
16-17	29.342911157312276	33.0	27.0	37.0	6.0	40.0
18-19	29.462802102847554	33.0	22.0	37.0	10.5	40.0
20-21	28.96306727781262	33.0	22.0	37.0	6.0	40.0
22-23	29.697418829237407	33.0	27.0	37.0	15.0	40.0
24-25	29.798538210401716	33.0	27.0	37.0	15.0	40.0
26-27	28.433648384045945	33.0	18.5	37.0	4.0	40.0
28-29	29.128501973184058	33.0	22.0	37.0	6.0	40.0
30-31	29.82488692253866	33.0	27.0	37.0	15.0	40.0
32-33	28.63275263712974	33.0	22.0	37.0	6.0	40.0
34-35	28.13900471668626	33.0	22.0	37.0	6.0	40.0
36-37	28.429980701715472	33.0	22.0	37.0	6.0	40.0
38-39	28.536563090468018	33.0	22.0	37.0	6.0	40.0
40-41	27.678381888247404	33.0	15.0	37.0	6.0	40.0
42-43	28.272139891389173	33.0	22.0	37.0	6.0	40.0
44-45	28.07485092730576	33.0	22.0	37.0	6.0	40.0
46-47	27.817200075015275	33.0	22.0	37.0	6.0	40.0
48-49	27.6057931151303	33.0	18.5	37.0	6.0	40.0
50-51	27.56430361827711	33.0	22.0	37.0	6.0	40.0
52-53	27.795273029387033	33.0	22.0	37.0	6.0	40.0
54-55	27.740232386030222	33.0	22.0	37.0	6.0	40.0
56-57	27.67153734933928	33.0	22.0	37.0	6.0	40.0
58-59	27.5156237459644	33.0	22.0	37.0	6.0	40.0
60-61	27.496668676484525	33.0	22.0	37.0	6.0	40.0
62-63	27.412728801630976	33.0	22.0	37.0	6.0	40.0
64-65	26.975539172291132	33.0	22.0	37.0	6.0	38.5
66-67	27.201065741209398	33.0	22.0	37.0	6.0	37.0
68-69	26.82279335106544	30.0	22.0	37.0	6.0	37.0
70-71	26.924713701782018	27.0	22.0	37.0	6.0	37.0
72-73	26.911535211807244	27.0	22.0	37.0	6.0	37.0
74-75	27.019166806143993	27.0	22.0	35.0	6.0	37.0
76-77	26.76517254521586	27.0	22.0	33.0	6.0	37.0
78-79	26.364811181309097	27.0	22.0	33.0	6.0	37.0
80-81	26.182460138052306	27.0	22.0	33.0	6.0	37.0
82-83	26.269774893677948	27.0	22.0	33.0	6.0	37.0
84-85	26.22543118659243	27.0	22.0	33.0	6.0	37.0
86-87	26.00412019382979	27.0	22.0	33.0	6.0	37.0
88-89	25.944362662558305	27.0	22.0	33.0	6.0	37.0
90-91	26.070719440853885	27.0	22.0	33.0	6.0	37.0
92-93	26.015476336915377	27.0	22.0	33.0	6.0	37.0
94-95	25.902892524485832	27.0	22.0	33.0	6.0	37.0
96-97	25.982071147552222	27.0	22.0	33.0	6.0	37.0
98-99	26.054417935910337	27.0	22.0	33.0	10.5	37.0
100	25.711146826269058	27.0	22.0	33.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.372467129240839
1101	2	11.328475975333284
1101	3	8.525618870410792
1101	4	12.868853847391758
1101	5	10.707470811282729
1101	6	10.074009042576542
1101	7	9.542437670420927
1101	8	9.080511800171255
1101	9	7.518659685491283
1101	10-11	7.871803676783333
1101	12-13	9.235654987056218
1101	14-15	8.04137381804988
1101	16-17	8.231673656799504
1101	18-19	8.403734540070467
1101	20-21	7.241256364660575
1101	22-23	7.481879059501786
1101	24-25	7.59596346629051
1101	26-27	8.506120310650797
1101	28-29	8.29422984004103
1101	30-31	5.560740037570561
1101	32-33	6.965687542337786
1101	34-35	8.862123103822636
1101	36-37	8.299940740338954
1101	38-39	8.328473689982996
1101	40-41	9.147193958628609
1101	42-43	8.735658481250123
1101	44-45	8.564343624729215
1101	46-47	7.529643147794392
1101	48-49	5.388435988884346
1101	50-51	6.821702043869468
1101	52-53	5.910205715003745
1101	54-55	4.1910133066424216
1101	56-57	6.3371030292546
1101	58-59	6.364595331951939
1101	60-61	6.784331432871902
1101	62-63	6.490395755766251
1101	64-65	6.955150001341149
1101	66-67	5.941324441156599
1101	68-69	6.949257408319898
1101	70-71	6.482436572831762
1101	72-73	5.59193292917271
1101	74-75	5.0870220325685835
1101	76-77	5.299274396571256
1101	78-79	5.304519742943292
1101	80-81	5.175516896048219
1101	82-83	4.693930373008001
1101	84-85	4.411541276640424
1101	86-87	4.190237845761814
1101	88-89	4.3112969744993315
1101	90-91	4.1668020102912
1101	92-93	4.002629415173995
1101	94-95	3.8856666548306897
1101	96-97	4.05412121326248
1101	98-99	3.6609016525968343
1101	100	3.8907443239302566
1102	1	6.894361849280962
1102	2	10.451129573512333
1102	3	8.106015173438898
1102	4	12.62709928402521
1102	5	10.606769764584303
1102	6	9.638945141366818
1102	7	6.809114546210537
1102	8	7.8622446340408025
1102	9	6.5950258156995964
1102	10-11	7.468700744854214
1102	12-13	9.179722615608622
1102	14-15	6.251891891877367
1102	16-17	7.247013775553487
1102	18-19	7.353443012893031
1102	20-21	6.947867760945169
1102	22-23	6.53989454441858
1102	24-25	6.99549577097368
1102	26-27	8.486497913833663
1102	28-29	7.75029680040905
1102	30-31	4.30595743145831
1102	32-33	4.501801200665582
1102	34-35	5.2473551247097525
1102	36-37	5.173889198987585
1102	38-39	4.580184140080409
1102	40-41	6.024811894761225
1102	42-43	6.950039276674492
1102	44-45	6.521145496779869
1102	46-47	5.48278281771157
1102	48-49	3.5465294399007803
1102	50-51	4.920222916158
1102	52-53	5.681709119091295
1102	54-55	5.227032030930133
1102	56-57	6.392417654235096
1102	58-59	6.178210321618241
1102	60-61	6.073498543993274
1102	62-63	4.397943437333172
1102	64-65	6.138437164051009
1102	66-67	3.813438599947485
1102	68-69	5.939550402721153
1102	70-71	5.289778015935855
1102	72-73	5.058799953253207
1102	74-75	4.315829172850776
1102	76-77	3.4278749439071845
1102	78-79	4.439690303154734
1102	80-81	4.382683987318519
1102	82-83	3.923593557026834
1102	84-85	3.0304683086221864
1102	86-87	3.364731675488091
1102	88-89	3.5005560690081943
1102	90-91	3.2512332463452296
1102	92-93	3.2736803802365806
1102	94-95	3.206977547404449
1102	96-97	3.4864498021219923
1102	98-99	3.239037819880064
1102	100	3.299146344337295
1103	1	6.541686575786056
1103	2	10.793475499971589
1103	3	7.748157791886431
1103	4	11.783602317583647
1103	5	10.096719078464805
1103	6	8.879264003997854
1103	7	7.852489587561799
1103	8	7.94189226963751
1103	9	7.019281955446086
1103	10-11	7.306975569004745
1103	12-13	8.724299456163198
1103	14-15	8.417973420384062
1103	16-17	7.778617722664638
1103	18-19	7.096339887968
1103	20-21	8.22708555276942
1103	22-23	6.799305583701454
1103	24-25	5.186472912747686
1103	26-27	5.798685896061283
1103	28-29	6.990405075764809
1103	30-31	6.004799043572547
1103	32-33	7.874056406085479
1103	34-35	7.012101304752328
1103	36-37	4.068890489932883
1103	38-39	6.192615522897153
1103	40-41	6.693674026436415
1103	42-43	7.445534575989672
1103	44-45	5.422815344735238
1103	46-47	6.279054763996168
1103	48-49	5.438905597096905
1103	50-51	6.345604956516006
1103	52-53	6.08582235944705
1103	54-55	4.868166208829635
1103	56-57	5.8265473891068815
1103	58-59	5.372871522350781
1103	60-61	4.806033571030365
1103	62-63	2.479732707080956
1103	64-65	4.905941278928427
1103	66-67	3.724348582364346
1103	68-69	4.880646609016786
1103	70-71	3.598736472756695
1103	72-73	4.27977155612885
1103	74-75	3.168490529601854
1103	76-77	3.466043904708748
1103	78-79	2.7593667805595175
1103	80-81	3.9828386570827696
1103	82-83	3.2592396266723966
1103	84-85	3.1418515598570274
1103	86-87	2.637284771297786
1103	88-89	2.895341041244887
1103	90-91	2.5960165592118543
1103	92-93	2.2349303138703576
1103	94-95	2.81903338405764
1103	96-97	2.855531972789457
1103	98-99	2.7618455612138177
1103	100	2.993967733483668
1104	1	6.279348390210085
1104	2	9.569550324726364
1104	3	7.317368004328582
1104	4	12.048615574896214
1104	5	9.310205624018717
1104	6	8.051734622301417
1104	7	3.985067707462221
1104	8	6.422630024352134
1104	9	6.6323152178737494
1104	10-11	7.125080888983593
1104	12-13	7.378262957630408
1104	14-15	7.115684097519296
1104	16-17	7.117091009858239
1104	18-19	6.868022541443587
1104	20-21	7.393933281693069
1104	22-23	6.499288945485176
1104	24-25	6.046367990703001
1104	26-27	8.273319409137873
1104	28-29	7.0868262981239845
1104	30-31	6.354521602014714
1104	32-33	7.535992962443395
1104	34-35	8.117851418511417
1104	36-37	6.551180952745966
1104	38-39	7.295145457279926
1104	40-41	7.124611301792992
1104	42-43	7.192794343144751
1104	44-45	6.644625678359766
1104	46-47	6.595155046199732
1104	48-49	5.492693380979048
1104	50-51	5.4639272438642195
1104	52-53	5.863240814462941
1104	54-55	4.654957840002432
1104	56-57	6.002878866380367
1104	58-59	3.8162570403341824
1104	60-61	4.11495535698991
1104	62-63	3.637789999053396
1104	64-65	2.682882555210231
1104	66-67	3.1731944318401055
1104	68-69	4.049222386435172
1104	70-71	2.3565451449344827
1104	72-73	2.2278814509186766
1104	74-75	3.0239518232748566
1104	76-77	3.7094798507368054
1104	78-79	4.216729954368425
1104	80-81	3.0166761754013116
1104	82-83	2.861032122061527
1104	84-85	2.784871819399932
1104	86-87	0.7157107185052567
1104	88-89	0.12051975800464731
1104	90-91	1.9932601543979054
1104	92-93	2.2436829795560307
1104	94-95	2.292172565984764
1104	96-97	2.6519049848343137
1104	98-99	2.46766551776237
1104	100	0.6706220214897236
1105	1	5.718325740279958
1105	2	-2.5127178354454003
1105	3	0.6229910089651831
1105	4	8.227951058052398
1105	5	8.303090919539382
1105	6	7.904173151708761
1105	7	5.125006723782786
1105	8	5.84645390268803
1105	9	4.985814041139189
1105	10-11	4.920929832164823
1105	12-13	4.977121667915945
1105	14-15	5.683088579966551
1105	16-17	5.91646501295028
1105	18-19	6.220897836804184
1105	20-21	7.008053918510345
1105	22-23	2.005440302838821
1105	24-25	4.019204074248908
1105	26-27	6.859253725076325
1105	28-29	5.879657059364028
1105	30-31	5.262653675268361
1105	32-33	7.039365122013116
1105	34-35	5.96585637855733
1105	36-37	6.096195883763308
1105	38-39	4.074435532400042
1105	40-41	5.328551447159384
1105	42-43	5.432386544735646
1105	44-45	6.036172048836264
1105	46-47	5.294128729032774
1105	48-49	4.911418140173652
1105	50-51	5.9423022987585625
1105	52-53	5.429912512130301
1105	54-55	5.16126811327679
1105	56-57	5.059220471234482
1105	58-59	2.0180960396402448
1105	60-61	4.381355213229767
1105	62-63	-0.9219749588248192
1105	64-65	-0.5906564863025316
1105	66-67	0.5107662495784346
1105	68-69	2.529035234604276
1105	70-71	2.7265486088621564
1105	72-73	2.507665256915569
1105	74-75	2.5077575690845
1105	76-77	1.647547680041228
1105	78-79	3.16193046514519
1105	80-81	1.4045027206790444
1105	82-83	0.7656513234917632
1105	84-85	1.18457562969596
1105	86-87	-0.47003428627076715
1105	88-89	1.5408939891307476
1105	90-91	1.352985538480457
1105	92-93	1.711985757174073
1105	94-95	1.12022358688791
1105	96-97	2.0844986715584817
1105	98-99	1.9314305082816432
1105	100	1.9707578759449191
1106	1	5.909970957095279
1106	2	10.15873397592873
1106	3	6.535657619063322
1106	4	11.511079538297128
1106	5	9.47016914678954
1106	6	9.219478344449463
1106	7	6.236342011756268
1106	8	7.234710331314584
1106	9	6.791583058004338
1106	10-11	5.385211072336482
1106	12-13	7.63223604567488
1106	14-15	7.463963768157672
1106	16-17	7.3651965767803524
1106	18-19	6.987086007270115
1106	20-21	7.945634414330858
1106	22-23	5.910403002381187
1106	24-25	4.731134160756618
1106	26-27	7.380984500703793
1106	28-29	5.850012622015964
1106	30-31	4.964530607343619
1106	32-33	7.08726090812489
1106	34-35	6.335375892866399
1106	36-37	5.688232591553145
1106	38-39	6.809551686689343
1106	40-41	6.3150627138937026
1106	42-43	5.8505396910390886
1106	44-45	3.999651315380664
1106	46-47	5.688779450865564
1106	48-49	5.577743243243074
1106	50-51	6.375962677880281
1106	52-53	5.378426086161689
1106	54-55	4.970131036899719
1106	56-57	4.598581597893187
1106	58-59	4.658843473168773
1106	60-61	4.6001086356456256
1106	62-63	4.6225092963579755
1106	64-65	4.736840822610233
1106	66-67	2.69276053200079
1106	68-69	2.8524908341741977
1106	70-71	2.43578637491051
1106	72-73	2.4050954129121322
1106	74-75	2.356562280336142
1106	76-77	3.177978607180769
1106	78-79	3.8927499063890494
1106	80-81	3.380777048071664
1106	82-83	1.8301675514251983
1106	84-85	2.345471918381527
1106	86-87	3.0957652844501666
1106	88-89	3.2443070581664237
1106	90-91	3.088310998603024
1106	92-93	2.618463817246763
1106	94-95	1.2070878885037146
1106	96-97	1.1692001519399327
1106	98-99	1.7271130051036039
1106	100	2.653942769421441
1107	1	5.932985741175905
1107	2	10.194192341585993
1107	3	5.883390025321685
1107	4	11.086754979594406
1107	5	9.199830716919138
1107	6	7.8755219894863195
1107	7	3.463872689799164
1107	8	6.005416380068709
1107	9	5.587251292801788
1107	10-11	4.370747469194125
1107	12-13	5.822957727559004
1107	14-15	5.830580346737431
1107	16-17	6.719464932179111
1107	18-19	6.140763658246961
1107	20-21	5.220849836240493
1107	22-23	5.547238720402682
1107	24-25	4.26126980025953
1107	26-27	6.840775929002483
1107	28-29	6.0955916326818524
1107	30-31	4.713609795483013
1107	32-33	4.453983668294995
1107	34-35	6.348343424626766
1107	36-37	6.064411529885138
1107	38-39	6.213444154552519
1107	40-41	7.406189626943785
1107	42-43	5.899289981786399
1107	44-45	3.7483652711171622
1107	46-47	3.0634719813822677
1107	48-49	3.6743913132859483
1107	50-51	4.715404651439421
1107	52-53	3.638620611238558
1107	54-55	5.000944319312499
1107	56-57	5.095154392952971
1107	58-59	2.875969548597702
1107	60-61	2.6197310204323827
1107	62-63	0.34975973096638313
1107	64-65	1.8473216077422556
1107	66-67	3.933370603891003
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2103	6	9.058065616356636
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2103	9	6.514664585017446
2103	10-11	7.460055411699965
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2103	16-17	7.912115285230843
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2103	54-55	4.6526926202303684
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2103	66-67	4.290212097892102
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2103	78-79	3.299176392402263
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2104	4	11.939234191622464
2104	5	9.967495334110918
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2206	98-99	1.352759061555286
2206	100	1.5320630685864174
2207	1	6.064853976766141
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2207	3	7.637854797953615
2207	4	11.842002378705072
2207	5	10.216030691749243
2207	6	9.163530368964466
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2207	8	6.662297987166621
2207	9	6.019451718592542
2207	10-11	6.862116160988737
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2207	14-15	8.248586261663181
2207	16-17	7.238854142683355
2207	18-19	4.03916877822347
2207	20-21	7.35509636489223
2207	22-23	6.193508856320122
2207	24-25	4.264945785031745
2207	26-27	3.6073350992348843
2207	28-29	4.897554753539307
2207	30-31	5.4383677974127025
2207	32-33	6.699310719641584
2207	34-35	6.189159165250977
2207	36-37	6.6999945538384935
2207	38-39	6.648461249107431
2207	40-41	3.814036866645104
2207	42-43	5.352666942940861
2207	44-45	3.596346697830807
2207	46-47	5.385589634918638
2207	48-49	2.198902204510045
2207	50-51	5.251111427385389
2207	52-53	2.5721252834006556
2207	54-55	2.8885904882444535
2207	56-57	5.181411104925452
2207	58-59	3.479475636566491
2207	60-61	3.4112079952205363
2207	62-63	4.24615597855427
2207	64-65	3.4038752196224458
2207	66-67	3.421849739095297
2207	68-69	3.295769685458172
2207	70-71	3.253929659537768
2207	72-73	3.562379795103979
2207	74-75	3.322361654519696
2207	76-77	3.9335864287880185
2207	78-79	4.038481845919836
2207	80-81	4.134458321268188
2207	82-83	3.6380260640320365
2207	84-85	3.0349401325326504
2207	86-87	3.269112628322894
2207	88-89	3.1044264838560487
2207	90-91	1.1959900259847096
2207	92-93	-1.0601532751613973
2207	94-95	1.0456507602037988
2207	96-97	-1.2202328009863486
2207	98-99	0.4620699195191591
2207	100	1.7882591892760722
2208	1	0.7914155760914134
2208	2	8.208439179703518
2208	3	6.291954144714776
2208	4	11.465650481738717
2208	5	9.744829149945797
2208	6	9.006794731471317
2208	7	7.310520940247741
2208	8	7.546193714441554
2208	9	6.494372351767506
2208	10-11	6.673438505079101
2208	12-13	6.852081002764475
2208	14-15	7.660794177589079
2208	16-17	7.004825981460424
2208	18-19	6.3708845945520665
2208	20-21	6.500132522084176
2208	22-23	5.725232137188044
2208	24-25	6.052410203639571
2208	26-27	8.021596111105335
2208	28-29	6.979440038928683
2208	30-31	6.03530861935392
2208	32-33	8.25685596574764
2208	34-35	6.27668611912733
2208	36-37	6.669383764273526
2208	38-39	7.125100654088211
2208	40-41	7.503129196360064
2208	42-43	7.084307083630673
2208	44-45	5.907549757824217
2208	46-47	4.618104362372566
2208	48-49	4.564010789223275
2208	50-51	5.883803647805841
2208	52-53	5.544366346052726
2208	54-55	6.3188702213818
2208	56-57	6.465494975250536
2208	58-59	5.751366156614182
2208	60-61	5.09789974236012
2208	62-63	3.2509278164717514
2208	64-65	3.2739084251294948
2208	66-67	2.7546426201875285
2208	68-69	5.257386246799619
2208	70-71	5.211291433461206
2208	72-73	4.514610346091903
2208	74-75	1.7828228714026793
2208	76-77	3.2147017700154343
2208	78-79	4.0019030433912235
2208	80-81	3.297402451877524
2208	82-83	1.2091201442794386
2208	84-85	1.7976315085310368
2208	86-87	0.9579090942901587
2208	88-89	0.529587808921125
2208	90-91	1.8882867351657495
2208	92-93	0.7835603344193025
2208	94-95	-1.2467128706897128
2208	96-97	1.1975902372240022
2208	98-99	0.07315767102547
2208	100	1.541675527528291
2209	1	4.9947975982869295
2209	2	9.262383664303393
2209	3	5.138868029119234
2209	4	-3.548368375593732
2209	5	0.3843784775510386
2209	6	3.9321399422241647
2209	7	2.066096908102203
2209	8	4.711756423621932
2209	9	4.516737359549513
2209	10-11	4.695250897935345
2209	12-13	6.891559935584944
2209	14-15	6.308921854445256
2209	16-17	5.905905977638934
2209	18-19	4.936671013815634
2209	20-21	5.645982209630542
2209	22-23	5.147940677300365
2209	24-25	4.83961845932328
2209	26-27	6.578104443071268
2209	28-29	5.838330367422909
2209	30-31	3.608806592978766
2209	32-33	5.822294935052625
2209	34-35	5.265786347358237
2209	36-37	5.838808714564102
2209	38-39	6.555111423953058
2209	40-41	6.910571160090239
2209	42-43	6.450763649809705
2209	44-45	6.192776233966601
2209	46-47	6.547761885948319
2209	48-49	4.915213109967198
2209	50-51	4.238178901866547
2209	52-53	4.537886237032701
2209	54-55	5.027737582898119
2209	56-57	4.860543410425084
2209	58-59	2.826950196605029
2209	60-61	3.796326310376692
2209	62-63	4.07088045432522
2209	64-65	4.837117974918609
2209	66-67	4.367084897610713
2209	68-69	3.5876959847951113
2209	70-71	3.4109360477135233
2209	72-73	0.32041580622268384
2209	74-75	1.8280026288069244
2209	76-77	3.1157364951246933
2209	78-79	3.369608349653774
2209	80-81	3.5062884747506295
2209	82-83	2.8269588054144243
2209	84-85	2.850312974281966
2209	86-87	1.232163792216042
2209	88-89	1.8415127646310303
2209	90-91	2.2029517215563104
2209	92-93	2.1212339097778674
2209	94-95	0.6324581114157155
2209	96-97	-0.0691307233093319
2209	98-99	0.09123130503221688
2209	100	-0.20442232464693433
2210	1	1.574275256437403
2210	2	-16.75672006717313
2210	3	-7.302384528649231
2210	4	-5.8488379586071595
2210	5	-0.1569834586366774
2210	6	3.0932139152581506
2210	7	-5.69877705300404
2210	8	-5.477326417362594
2210	9	-1.8018295341537467
2210	10-11	-0.6638175718630634
2210	12-13	3.341296181414645
2210	14-15	-3.4340619014685423
2210	16-17	-0.2954295574781689
2210	18-19	-0.42906847104999457
2210	20-21	2.5087676487302737
2210	22-23	2.7276953000413116
2210	24-25	2.3850479952056567
2210	26-27	4.138621653262117
2210	28-29	3.5104263214381284
2210	30-31	2.766618747817411
2210	32-33	4.574452342822216
2210	34-35	2.49610255063876
2210	36-37	4.096505249395339
2210	38-39	3.2834144561645964
2210	40-41	4.166526498938104
2210	42-43	2.764886518626259
2210	44-45	3.5059309270219075
2210	46-47	2.9364805825126616
2210	48-49	2.9381439328829373
2210	50-51	1.9109893435634078
2210	52-53	2.2740554354729845
2210	54-55	2.8144458761565474
2210	56-57	2.9610364394042676
2210	58-59	2.0686718902143184
2210	60-61	2.9146861914061866
2210	62-63	2.232033671051944
2210	64-65	2.9907338384938704
2210	66-67	2.8931184394159146
2210	68-69	3.343744147261674
2210	70-71	2.5333703772391587
2210	72-73	1.0658953265881692
2210	74-75	0.42513233140937245
2210	76-77	-0.5917790830744281
2210	78-79	0.30807977624218097
2210	80-81	1.0850872778154859
2210	82-83	1.0387778080189953
2210	84-85	-0.7066279376950249
2210	86-87	-0.3365483972808363
2210	88-89	-1.413708669829962
2210	90-91	-1.2884479018678725
2210	92-93	-0.8157004198183735
2210	94-95	-0.014724844736939247
2210	96-97	0.24840673198576368
2210	98-99	0.0420782805426434
2210	100	0.006222567443963101
2211	1	1.0384842325337793
2211	2	-16.75672006717313
2211	3	-7.872590189440746
2211	4	-18.808500494858148
2211	5	-20.69560723928524
2211	6	-13.698029406185274
2211	7	-12.099710891816718
2211	8	-17.9388024654056
2211	9	-16.200761752097485
2211	10-11	-10.4810172513163
2211	12-13	-21.972571469521288
2211	14-15	-17.464139465025717
2211	16-17	-15.150671710061918
2211	18-19	-18.660935577248047
2211	20-21	-8.862767068130065
2211	22-23	-4.643027399805547
2211	24-25	-2.1355098290455494
2211	26-27	-16.905164947383597
2211	28-29	-4.042877024508236
2211	30-31	-5.480771163890225
2211	32-33	-1.5030597602304212
2211	34-35	-6.993209363637565
2211	36-37	-7.871017106842366
2211	38-39	-3.1857767158653054
2211	40-41	-6.585445994536247
2211	42-43	-2.3554081240032794
2211	44-45	-0.47163015795771557
2211	46-47	0.7209939360330075
2211	48-49	0.45177174688437916
2211	50-51	-5.4925261404911225
2211	52-53	-1.5698631323728023
2211	54-55	-0.8982373548302753
2211	56-57	-8.013519647981681
2211	58-59	-2.6015683213985596
2211	60-61	-1.565406367840474
2211	62-63	-0.6361373663777421
2211	64-65	-4.221145758694366
2211	66-67	-3.16091467394072
2211	68-69	-0.1988658092944533
2211	70-71	-0.5061577641011574
2211	72-73	-1.2120839524998317
2211	74-75	-1.6710941012777774
2211	76-77	-1.8062242861903144
2211	78-79	-1.1531185991934265
2211	80-81	-0.9118077248132543
2211	82-83	-1.4906756765156004
2211	84-85	-1.4994685609750746
2211	86-87	-1.9533297885283787
2211	88-89	-2.601498072522478
2211	90-91	-2.2468279773270687
2211	92-93	-2.1092139244076726
2211	94-95	-2.119556048705178
2211	96-97	-3.132079932457927
2211	98-99	-2.8060827928373477
2211	100	-2.2928493935593757
2212	1	-20.7347149887511
2212	2	-16.75672006717313
2212	3	-19.62956617905788
2212	4	-18.808500494858148
2212	5	-20.69560723928524
2212	6	-21.50367509080565
2212	7	-21.981930229908865
2212	8	-22.579620116281657
2212	9	-23.850339948074126
2212	10-11	-11.125982204592155
2212	12-13	-21.972571469521288
2212	14-15	-23.328999945233374
2212	16-17	-23.69577229409826
2212	18-19	-18.26698356909905
2212	20-21	-23.36455162361352
2212	22-23	-18.40792623334383
2212	24-25	-24.86409516324412
2212	26-27	-17.013048405305014
2212	28-29	-17.769864199997134
2212	30-31	-6.114279479561475
2212	32-33	-16.974220100284228
2212	34-35	-13.510594348050457
2212	36-37	-17.324135271863714
2212	38-39	-23.301610589973027
2212	40-41	-9.934342752822692
2212	42-43	-13.814883193512237
2212	44-45	-16.769431171708323
2212	46-47	-16.800368579978528
2212	48-49	-17.34454346255537
2212	50-51	-8.652742583805228
2212	52-53	-2.1862263526646686
2212	54-55	-23.130716264629108
2212	56-57	-22.975557285879148
2212	58-59	-12.294754791285307
2212	60-61	-6.253560434635986
2212	62-63	-3.135473360142072
2212	64-65	-16.89982849424309
2212	66-67	-17.174560673318638
2212	68-69	-14.335908125182977
2212	70-71	-9.463147715404613
2212	72-73	-17.088151887256622
2212	74-75	-6.133883212484264
2212	76-77	-10.88469990962298
2212	78-79	-4.567381453135599
2212	80-81	-2.4622258318812094
2212	82-83	-2.8475940018898065
2212	84-85	-5.253786150510585
2212	86-87	-5.020157420770953
2212	88-89	-3.374621289361098
2212	90-91	-4.334117225052406
2212	92-93	-5.274283292922252
2212	94-95	-3.8630114049739532
2212	96-97	-5.583174375830691
2212	98-99	-3.903946365682444
2212	100	-4.0540799798965
2213	1	-20.7347149887511
2213	2	-16.75672006717313
2213	3	-19.62956617905788
2213	4	-18.808500494858148
2213	5	-20.69560723928524
2213	6	-21.50367509080565
2213	7	-10.40399133814294
2213	8	-10.35072317169336
2213	9	-4.348527415707476
2213	10-11	-17.44793555310437
2213	12-13	-16.013482914368517
2213	14-15	-23.328999945233374
2213	16-17	-17.699397358831558
2213	18-19	-18.26312000892749
2213	20-21	-17.41115959875593
2213	22-23	-19.49700403230289
2213	24-25	-7.447212718914759
2213	26-27	-22.929662027201896
2213	28-29	-23.82110544761012
2213	30-31	-19.16960613590158
2213	32-33	-23.01782290104807
2213	34-35	-17.590595969885364
2213	36-37	-23.390971681793694
2213	38-39	-11.174215457917098
2213	40-41	-16.861996224719626
2213	42-43	-22.89158593062236
2213	44-45	-16.820666980240823
2213	46-47	-16.595311949261436
2213	48-49	-5.895533029080436
2213	50-51	-22.974193888706566
2213	52-53	-17.45048770410805
2213	54-55	-17.348738015017506
2213	56-57	-17.11123828018262
2213	58-59	-17.565808235690156
2213	60-61	-18.928777940168192
2213	62-63	-17.127560981421208
2213	64-65	-17.211170361548646
2213	66-67	-8.396643438982284
2213	68-69	-22.383648417990106
2213	70-71	-17.115741283246926
2213	72-73	-9.130486558164232
2213	74-75	-4.120106021614401
2213	76-77	-2.929325092697482
2213	78-79	-4.489985645315127
2213	80-81	-3.0796943124097957
2213	82-83	-3.0465239204914987
2213	84-85	-2.802124247709262
2213	86-87	-2.9270846996055795
2213	88-89	-2.318740670914572
2213	90-91	-2.404626168054474
2213	92-93	-3.490473226931371
2213	94-95	-3.451295427823368
2213	96-97	-3.0296469013869505
2213	98-99	-3.4672261114367515
2213	100	-3.0368226016190007
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	714.0
3	634.0
4	2921.0
5	11525.0
6	18248.0
7	20270.0
8	18740.0
9	19249.0
10	22913.0
11	29122.0
12	38545.0
13	49891.0
14	43621.0
15	48580.0
16	53496.0
17	56546.0
18	55694.0
19	55746.0
20	55251.0
21	55067.0
22	58008.0
23	62964.0
24	67683.0
25	73971.0
26	79756.0
27	85759.0
28	91012.0
29	94616.0
30	100900.0
31	109700.0
32	124404.0
33	145729.0
34	168574.0
35	186318.0
36	187337.0
37	145730.0
38	40194.0
39	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.26721890174188	20.63434932343007	13.809416662713424	24.28901511211463
2	15.245258338783518	20.89819053847831	39.94953925994459	23.907011862793578
3	18.658124362973485	25.950519480019558	29.45264158295235	25.93871457405461
4	12.38839687957858	17.236698119859913	38.60735393591488	31.767551064646625
5	13.193229702962109	37.409534784452596	35.70078694571605	13.696448566869249
6	30.878102377857395	37.52322430574067	18.62261120631995	12.976062110081985
7	26.38125341190633	31.37352850603995	24.38139513724612	17.863822944807602
8	23.793375809594018	37.01411194776473	21.627964123356676	17.564548119284574
9	24.469228895807717	17.12210429665987	22.13531258194186	36.27335422559055
10-11	23.34255451756408	27.302673093522138	29.175329279981778	20.17944310893201
12-13	24.153953423511364	24.931596708966673	28.946858178775624	21.967591688746342
14-15	21.534488397309183	26.589451933752905	26.314444613733805	25.561615055204108
16-17	21.17187914969058	30.070862021074007	27.253600353178125	21.503658476057293
18-19	21.247730196468982	29.148207967093853	28.685212199034982	20.918849637402186
20-21	22.434893006278976	28.52480679569915	27.82935414873701	21.210946049284864
22-23	21.54740292158127	28.586981115859878	28.234488256244756	21.6311277063141
24-25	21.811013578346465	28.534393051499745	27.777255436633926	21.87733793351986
26-27	21.7529440802324	28.422715230376944	27.90548135179095	21.918859337599706
28-29	21.604870149420364	28.282275464987283	28.378083299029516	21.734771086562837
30-31	22.09744962612979	28.7687040005371	27.69654411332757	21.437302260005538
32-33	21.358832404043877	28.689614104253025	28.19788643331013	21.75366705839296
34-35	21.425332437238165	28.34039495286301	28.05702108962142	22.177251520277398
36-37	21.964476008294927	28.726361695884812	27.674254106770313	21.63490818904995
38-39	21.802857241266146	28.544644207592512	27.84469946638787	21.80779908475347
40-41	21.84605967584543	28.437460337017118	27.556349561596384	22.160130425541073
42-43	21.694839033031013	28.56507164386101	28.045572121029032	21.69451720207894
44-45	22.090270915744785	28.148565383596136	27.9464112327412	21.814752467917874
46-47	21.935852354618774	28.55584318896578	28.028967211494887	21.47933724492056
48-49	21.813540827725962	28.344707309039986	28.06831257579275	21.773439287441303
50-51	22.043368687985154	28.231302820101174	27.911678034421428	21.813650457492244
52-53	21.61028497247648	28.32200152090104	28.007987357063563	22.059726149558912
54-55	21.991647387504397	28.386515917146955	27.716163379062152	21.905673316286496
56-57	22.071310822355414	28.256100545008266	27.67642070879015	21.996167923846166
58-59	21.85264366190809	27.922962651522308	28.0720579129458	22.1523357736238
60-61	22.18525220576274	28.2731322082448	27.705465897675417	21.836149688317047
62-63	21.75626079222914	28.384856764017268	27.9800065444618	21.878875899291792
64-65	21.782377263849103	27.96263548076726	28.056993949708815	22.197993305674817
66-67	21.902149345783975	28.209993126551947	27.770835526438002	22.117022001226076
68-69	22.35929928473958	28.055271604502018	27.691557988823885	21.89387112193452
70-71	22.28177109488509	28.11451752111061	27.572019431351464	22.03169195265284
72-73	22.04851650533992	28.22498149634746	27.87500309502621	21.85149890328642
74-75	22.218079172462364	27.966824732138633	27.609547617553453	22.20554847784555
76-77	22.18694781061529	28.211816406747865	27.707857721857142	21.893378060779707
78-79	22.203719794091608	28.004073904062427	27.85484769992459	21.937358601921375
80-81	22.209856147575955	27.934016837097126	27.710605068400064	22.145521946926856
82-83	21.812053959275882	28.097859573149357	28.043535564862054	22.04655090271271
84-85	22.018928306941813	27.863236323193814	27.62226273687663	22.495572632987745
86-87	22.258340569383623	27.99668715181362	27.691618242869538	22.05335403593322
88-89	22.069145115526247	27.658554044962447	27.99667023098749	22.27563060852382
90-91	22.232293401937902	27.78780622582428	27.75193861213386	22.22796176010396
92-93	21.90171333582329	27.98265743683083	27.962667642147064	22.15296158519881
94-95	21.783762802430708	27.62142405086371	27.986484263514317	22.608328883191266
96-97	22.012533373968385	27.760738434005056	27.914133054939995	22.312595137086564
98-99	21.938613502080994	27.64103617155364	27.921816499879306	22.49853382648606
100	22.132581321779377	27.45664330198197	27.788908639587284	22.62186673665137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	280.0
1	334.0
2	330.0
3	258.5
4	270.5
5	358.0
6	519.5
7	750.5
8	1084.0
9	1573.0
10	2243.0
11	2947.5
12	3705.0
13	4518.0
14	5316.5
15	6131.5
16	6881.5
17	7603.0
18	8311.0
19	8995.0
20	9651.5
21	10480.0
22	11529.5
23	12885.0
24	14916.5
25	17412.5
26	20786.5
27	25363.0
28	31028.0
29	37557.5
30	44496.0
31	51308.0
32	58215.5
33	65145.5
34	71534.0
35	77206.5
36	82540.5
37	87010.5
38	90560.0
39	92989.5
40	93575.5
41	93770.0
42	92483.5
43	89725.5
44	86922.5
45	83944.5
46	80994.0
47	77963.0
48	75057.5
49	73113.0
50	72242.5
51	68670.0
52	62166.5
53	56360.5
54	51753.5
55	47469.5
56	43053.5
57	38865.0
58	35030.0
59	31401.5
60	27527.0
61	23698.5
62	20134.5
63	16639.5
64	13484.5
65	10999.5
66	8829.5
67	6960.5
68	5476.5
69	4177.5
70	3214.5
71	2460.0
72	1803.5
73	1328.0
74	976.0
75	722.0
76	510.0
77	334.0
78	239.0
79	166.0
80	107.0
81	76.5
82	54.5
83	37.0
84	28.0
85	22.0
86	14.0
87	9.0
88	3.5
89	2.5
90	3.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	7.332218859082191
2	24.15108721735676
3	5.706161940233797
4	22.787301446463896
5	12.224505393235315
6	9.37239236215439
7	7.799289774732355
8	8.089348839178946
9	4.946208804615456
10-11	6.526510438617541
12-13	9.621213190589213
14-15	6.955670408692092
16-17	7.365895071375421
18-19	6.371599862068687
20-21	7.493824347296527
22-23	5.440966809773765
24-25	4.986842078729741
26-27	9.148596790878788
28-29	5.8954948487494425
30-31	3.88446034373935
32-33	8.09410787277248
34-35	6.83496034474762
36-37	6.462828116209147
38-39	5.73949534078512
40-41	7.690255475409308
42-43	6.012474314326103
44-45	6.0231014783252235
46-47	5.307492049792397
48-49	3.752901296433346
50-51	6.133446528426152
52-53	4.032454189260313
54-55	4.607672126783881
56-57	4.7319716313200875
58-59	3.308516451938802
60-61	3.8063799281708577
62-63	3.0009336578617827
64-65	4.407288580940877
66-67	2.3040982135475168
68-69	4.698920546320924
70-71	2.220714298677755
72-73	1.454267905359761
74-75	0.7074222775202209
76-77	1.1134323723177502
78-79	1.5960911395263955
80-81	1.9222664292527307
82-83	0.7024010937711106
84-85	1.1285160889616637
86-87	0.46493338361238884
88-89	0.7284951169492175
90-91	0.3750763764395572
92-93	0.3320837509250876
94-95	0.4601340192256891
96-97	0.48409051036602213
98-99	0.42278770475439553
100	0.4187949562310067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2479495.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.25027625339855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.1618006464029	92.54688352847513
2	2.1930790634272506	4.177827732739804
3	0.3663909298049933	1.046965118419955
4	0.10492386276904372	0.3997610765730038
5	0.06192289383859063	0.29490863722678146
6	0.030166982730075655	0.17240480632627259
7	0.017641062251249773	0.11762212369844592
8	0.01289523386810172	0.09826196706310959
9	0.009650754079991124	0.08273132929554909
>10	0.03952413093703787	0.6074421220855201
>50	6.82195358618104E-4	0.04451309801236165
>100	0.001109500055531445	0.21018673906088486
>500	1.2764668609496714E-4	0.07011333685236064
>1k	8.509779071373975E-5	0.1303783841708949
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	4.033079316554379E-5	0.0	0.0	0.0	0.0
10-11	4.033079316554379E-5	0.0	0.0	2.0165396582771896E-5	0.0
12-13	4.033079316554379E-5	0.0	0.0	1.0082698291385948E-4	0.0
14-15	4.033079316554379E-5	0.0	0.0	1.6132317266217517E-4	0.0
16-17	4.033079316554379E-5	0.0	0.0	2.0165396582771895E-4	0.0
18-19	6.049618974831569E-5	0.0	0.0	2.0165396582771895E-4	0.0
20-21	8.066158633108758E-5	0.0	0.0	3.226463453243503E-4	0.0
22-23	8.066158633108758E-5	0.0	0.0	4.033079316554379E-4	0.0
24-25	8.066158633108758E-5	0.0	0.0	5.041349145692974E-4	0.0
26-27	8.066158633108758E-5	0.0	0.0	6.251272940659288E-4	0.0
28-29	8.066158633108758E-5	0.0	0.0	8.267812598936477E-4	0.0
30-31	8.066158633108758E-5	0.0	0.0	0.002298855210435996	0.0
32-33	8.066158633108758E-5	0.0	0.0	0.006432761509904235	0.0
34-35	1.0082698291385948E-4	0.0	0.0	0.012946184606139556	0.0
36-37	1.2099237949663137E-4	0.0	0.0	0.023351529242849854	0.0
38-39	1.2099237949663137E-4	0.0	0.0	0.03853607286967709	0.0
40-41	1.2099237949663137E-4	0.0	0.0	0.05706807232924446	0.0
42-43	1.2099237949663137E-4	0.0	0.0	0.0746926289425871	0.0
44-45	1.2099237949663137E-4	0.0	0.0	0.09616877630323917	4.033079316554379E-5
46-47	1.2099237949663137E-4	0.0	0.0	0.12375503882847111	4.033079316554379E-5
48-49	1.2099237949663137E-4	0.0	0.0	0.15515256130784696	4.033079316554379E-5
50-51	1.2099237949663137E-4	0.0	0.0	0.1875583536163614	4.033079316554379E-5
52-53	1.2099237949663137E-4	8.066158633108758E-5	0.0	0.22050861163261068	4.033079316554379E-5
54-55	1.2099237949663137E-4	8.066158633108758E-5	0.0	0.261141885746896	4.033079316554379E-5
56-57	1.2099237949663137E-4	8.066158633108758E-5	0.0	0.30820792137108566	4.033079316554379E-5
58-59	1.2099237949663137E-4	8.066158633108758E-5	0.0	0.3622108534197488	4.033079316554379E-5
60-61	1.6132317266217517E-4	8.066158633108758E-5	0.0	0.41776652100528533	4.033079316554379E-5
62-63	1.6132317266217517E-4	8.066158633108758E-5	0.0	0.4758831939568339	4.033079316554379E-5
64-65	1.6132317266217517E-4	8.066158633108758E-5	0.0	0.5436590918715303	4.033079316554379E-5
66-67	1.6132317266217517E-4	8.066158633108758E-5	0.0	0.624219851219704	6.049618974831569E-5
68-69	1.6132317266217517E-4	8.066158633108758E-5	0.0	0.7129475961839004	8.066158633108758E-5
70-71	1.6132317266217517E-4	8.066158633108758E-5	0.0	0.8183924549152146	8.066158633108758E-5
72-73	1.8148856924494707E-4	8.066158633108758E-5	0.0	0.9284753548605664	1.2099237949663137E-4
74-75	2.0165396582771895E-4	8.066158633108758E-5	0.0	1.0497702153059394	1.2099237949663137E-4
76-77	2.0165396582771895E-4	8.066158633108758E-5	0.0	1.1869352428619537	1.2099237949663137E-4
78-79	2.0165396582771895E-4	8.066158633108758E-5	0.0	1.3377925746976702	1.4115777607940326E-4
80-81	2.0165396582771895E-4	8.066158633108758E-5	0.0	1.5075852139246098	1.6132317266217517E-4
82-83	2.0165396582771895E-4	8.066158633108758E-5	0.0	1.6963333259393547	1.6132317266217517E-4
84-85	2.0165396582771895E-4	1.2099237949663137E-4	0.0	1.8873802931645356	1.6132317266217517E-4
86-87	2.0165396582771895E-4	1.2099237949663137E-4	0.0	2.0985119953861573	1.6132317266217517E-4
88	2.0165396582771895E-4	1.2099237949663137E-4	0.0	2.278084851955741	1.6132317266217517E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	190	0.0013122043	17.557753	3
GTATAGG	815	0.0	17.31737	1
GTACATG	8135	0.0	17.123005	1
GTACCGT	235	3.2721764E-5	17.04441	6
TACATGG	8450	0.0	16.4818	2
GTACGGA	225	0.0021021897	16.363659	1
GTACTAG	575	1.1459633E-10	16.007927	1
GTATTAG	1055	0.0	15.704458	1
GTACAGA	1270	0.0	14.978546	1
AACACCG	375	5.3700387E-7	14.679252	5
GTATATA	1550	0.0	14.648113	1
ACATGGG	8925	0.0	14.577359	3
GTATAAC	590	2.8194336E-9	14.560883	1
TACACTG	1610	0.0	14.297973	5
TCTATAC	825	0.0	14.152613	3
ATAGCGT	215	0.0059878835	13.982121	8
GTATTAA	1255	0.0	13.69071	1
GTACTGG	1090	0.0	13.511278	1
TACCGTA	225	0.008348518	13.297245	7
TATACAG	1355	0.0	13.295531	5
>>END_MODULE