FastQCFastQC Report
Wed 25 May 2016
SRR1295104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479495
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49990.20161363503455343No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38860.15672546224130315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGT1652.6553644E-622.7982714
GACCGAT1200.00509208719.565787
TAATACG2502.4189103E-718.8085754
GTATAGA9200.017.7507671
TAGACCG1650.001567200417.0987035
GTCCTAA9050.016.9513451
GTATCAA86600.016.0004121
TATACTG13200.015.3175885
CATGGGG38500.015.2666994
CAACGCA92200.015.1468185
TCAACGC92850.015.1420674
TCCGTAC2509.506626E-515.0462523
ATCAACG93500.015.0361943
GCTATAC6300.014.9268363
AACGCAG93350.014.8923996
GTACATG110000.014.8460961
GAGTACT28000.014.59048212-13
GTACAGG12950.014.5212781
TATACCG1950.00477386714.4681345
TATCAAC96900.014.390772
Produced by FastQC (version 0.11.5)