FastQCFastQC Report
Wed 25 May 2016
SRR1295103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568930
Sequences flagged as poor quality0
Sequence length100
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGTC600.00301622533.4613468
TATAGCG700.0065114728.5911355
GTACCGT750.0090057526.752846
AACCGTC1150.00280080521.6917867
GTTAGAC2851.4901889E-719.6923733
GTACATG44600.019.5471531
TACATGG45300.018.2836882
CGCGTCC700.00961578717.52453622-23
ACATGGG46650.016.3617653
GTATTAT10250.016.2869261
GTATAGG4302.300767E-715.8169761
GTCCTGG9950.015.5351761
TTAGACT8600.015.0103454
CACACCG2000.003737138815.0103455
GTGTAGG4956.931077E-814.9890911
GTGTATA9550.014.8909941
GTCTTAG5451.4077159E-814.7484411
CTAATAC6501.2732926E-1114.67839053
CATGGGG16850.014.6559544
CTAGGAC4609.2470145E-814.6408513
Produced by FastQC (version 0.11.5)