##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295103_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1568930 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.543091151294195 33.0 27.0 33.0 15.0 33.0 2 22.795437017585233 33.0 6.0 33.0 2.0 33.0 3 25.112829125582405 33.0 15.0 33.0 15.0 33.0 4 25.34517409954555 33.0 6.0 37.0 2.0 37.0 5 27.083011351685542 33.0 15.0 37.0 2.0 37.0 6 27.953224171887847 33.0 15.0 37.0 6.0 37.0 7 27.900866832809623 33.0 15.0 37.0 6.0 37.0 8 28.53591747241751 33.0 22.0 37.0 6.0 37.0 9 29.588995047580198 37.0 27.0 37.0 15.0 37.0 10-11 29.124154041289287 35.0 24.5 37.0 10.5 37.0 12-13 27.944353157884674 33.0 15.0 37.0 4.0 37.0 14-15 29.7538959673153 33.0 27.0 37.0 10.5 40.0 16-17 30.194366542803056 37.0 27.0 40.0 6.0 40.0 18-19 30.33890868298777 37.0 27.0 40.0 10.5 40.0 20-21 29.890958168943165 35.0 27.0 40.0 6.0 40.0 22-23 30.61273829935051 37.0 27.0 40.0 15.0 40.0 24-25 30.768066134244357 37.0 27.0 40.0 15.0 40.0 26-27 29.352413428260025 33.0 24.5 40.0 4.0 40.0 28-29 30.05881078187045 35.0 27.0 40.0 10.5 40.0 30-31 30.731607528697904 37.0 27.0 40.0 15.0 40.0 32-33 29.547753245842706 33.0 27.0 40.0 6.0 40.0 34-35 29.174036126532094 33.0 22.0 37.0 6.0 40.0 36-37 29.47844964402491 33.0 27.0 37.0 6.0 40.0 38-39 29.607162524778033 33.0 24.5 37.0 6.0 40.0 40-41 28.686607433091343 33.0 22.0 37.0 6.0 40.0 42-43 29.337210710484214 33.0 22.0 37.0 6.0 40.0 44-45 29.25942075172251 33.0 22.0 37.0 6.0 40.0 46-47 28.970328822828296 33.0 22.0 37.0 6.0 40.0 48-49 28.917031033889337 33.0 22.0 37.0 6.0 40.0 50-51 28.88563001536079 33.0 22.0 37.0 6.0 40.0 52-53 29.067293951929024 33.0 22.0 37.0 6.0 40.0 54-55 29.073261394708496 33.0 22.0 37.0 6.0 40.0 56-57 29.074418871460168 33.0 22.0 37.0 6.0 40.0 58-59 28.954068696500162 33.0 22.0 37.0 10.5 40.0 60-61 28.91105562389654 33.0 22.0 37.0 10.5 40.0 62-63 28.858110304475026 33.0 22.0 37.0 10.5 40.0 64-65 28.458600128750167 33.0 22.0 37.0 6.0 40.0 66-67 28.667183048319558 33.0 22.0 37.0 10.5 40.0 68-69 28.24827015864315 33.0 22.0 37.0 10.5 40.0 70-71 28.38901448758071 33.0 22.0 37.0 10.5 38.5 72-73 28.441996456183514 33.0 22.0 37.0 10.5 37.0 74-75 28.511207319638224 33.0 22.0 37.0 15.0 37.0 76-77 28.300852491825637 33.0 22.0 37.0 15.0 37.0 78-79 27.84846328389412 33.0 22.0 37.0 15.0 37.0 80-81 27.648132485196918 33.0 22.0 37.0 10.5 37.0 82-83 27.745773552676027 33.0 22.0 35.0 15.0 37.0 84-85 27.698796632099583 33.0 22.0 33.0 15.0 37.0 86-87 27.45307120139203 27.0 22.0 33.0 15.0 37.0 88-89 27.410863773399704 27.0 22.0 33.0 15.0 37.0 90-91 27.54403606279439 27.0 22.0 33.0 15.0 37.0 92-93 27.46807091457235 27.0 22.0 33.0 15.0 37.0 94-95 27.344117647058823 27.0 22.0 33.0 15.0 37.0 96-97 27.424561643922928 27.0 22.0 33.0 15.0 37.0 98-99 27.48778530590912 27.0 22.0 33.0 15.0 37.0 100 27.154671655204503 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.372465730730681 1101 2 11.301714418592255 1101 3 8.575564428666684 1101 4 13.122532027303464 1101 5 10.983881536436801 1101 6 10.041313097740893 1101 7 9.645146276151056 1101 8 9.253183052240992 1101 9 7.513176725490947 1101 10-11 7.907710236307036 1101 12-13 9.259702888777174 1101 14-15 8.155146426891132 1101 16-17 8.37570569497829 1101 18-19 8.40760435890257 1101 20-21 7.481365599908585 1101 22-23 7.6399982997652 1101 24-25 7.438189596687646 1101 26-27 8.660767139508152 1101 28-29 8.368518623476561 1101 30-31 5.50127138920395 1101 32-33 7.062479190620444 1101 34-35 8.896778812730119 1101 36-37 8.38027824137842 1101 38-39 8.408558503956744 1101 40-41 9.168625819143745 1101 42-43 8.72488631566528 1101 44-45 8.657264588058052 1101 46-47 7.695259184671972 1101 48-49 5.823984047506421 1101 50-51 7.113929719512267 1101 52-53 6.1155375109594345 1101 54-55 4.285348236225989 1101 56-57 6.478458510033953 1101 58-59 6.480517942790755 1101 60-61 6.928431167242621 1101 62-63 6.539712969396621 1101 64-65 7.020377335451972 1101 66-67 5.996843992179475 1101 68-69 7.0220579662978295 1101 70-71 6.548524802517701 1101 72-73 5.761892117062192 1101 74-75 5.05575523656082 1101 76-77 5.215771436223644 1101 78-79 5.368546247967924 1101 80-81 5.232037740788524 1101 82-83 4.64994747654281 1101 84-85 4.346178678513283 1101 86-87 4.291382129922447 1101 88-89 4.216666228695669 1101 90-91 4.10828938082269 1101 92-93 4.030072330605794 1101 94-95 3.809848112450112 1101 96-97 4.081299557985783 1101 98-99 3.744255624735292 1101 100 4.02795667273363 1102 1 6.669957452542619 1102 2 10.371464784947925 1102 3 8.104504735296572 1102 4 12.687726788630641 1102 5 10.670048219310122 1102 6 9.534751791382252 1102 7 6.774803087466374 1102 8 7.990456820151472 1102 9 6.657403830180808 1102 10-11 7.5120879946368255 1102 12-13 8.972786776122167 1102 14-15 6.311315020443619 1102 16-17 7.298021830493209 1102 18-19 7.457152875652579 1102 20-21 7.0514859897553706 1102 22-23 6.683201314543961 1102 24-25 7.057719211361661 1102 26-27 8.564690940940821 1102 28-29 7.762398564586697 1102 30-31 4.28242373345374 1102 32-33 4.625016572546446 1102 34-35 5.014970610798631 1102 36-37 5.24956859466641 1102 38-39 4.6101183517165545 1102 40-41 6.441507652167449 1102 42-43 7.011805072563767 1102 44-45 6.574202758821595 1102 46-47 5.703844702065123 1102 48-49 3.68696438286473 1102 50-51 5.146973465535243 1102 52-53 6.012768646841131 1102 54-55 5.530952138788173 1102 56-57 6.437226053736474 1102 58-59 6.161005899541166 1102 60-61 6.250394850690853 1102 62-63 4.528493956091811 1102 64-65 6.372373142193599 1102 66-67 4.046936711297693 1102 68-69 6.157508315082072 1102 70-71 5.401171690243121 1102 72-73 4.942860217250026 1102 74-75 4.215902478273673 1102 76-77 3.431304148927442 1102 78-79 4.382595198885642 1102 80-81 4.46984078388628 1102 82-83 3.9578936841005685 1102 84-85 3.0362412703154433 1102 86-87 3.457586309874305 1102 88-89 3.7318174879526538 1102 90-91 3.4732018904827093 1102 92-93 3.238459870930509 1102 94-95 3.165464997394814 1102 96-97 3.340755969764434 1102 98-99 3.246107789347125 1102 100 3.512430357393093 1103 1 6.743982529649621 1103 2 11.016984120340933 1103 3 7.964624827763934 1103 4 12.395200565273647 1103 5 10.671258125630676 1103 6 9.30518194250936 1103 7 8.230473253487322 1103 8 8.151698048875652 1103 9 7.0362755958069165 1103 10-11 7.52316955932692 1103 12-13 8.886059077856675 1103 14-15 8.782760141272927 1103 16-17 8.331164805232103 1103 18-19 7.509724417086314 1103 20-21 8.599471136029777 1103 22-23 7.159030743476901 1103 24-25 5.584585235709778 1103 26-27 6.387945384022025 1103 28-29 7.434544864278969 1103 30-31 6.410458679908302 1103 32-33 8.19321849043326 1103 34-35 7.455007566351682 1103 36-37 4.679166434885527 1103 38-39 6.756487978751483 1103 40-41 7.331676760010868 1103 42-43 7.955313783453271 1103 44-45 6.111832545799597 1103 46-47 6.708653327585385 1103 48-49 6.062246236537135 1103 50-51 6.840432820966921 1103 52-53 6.412948018178177 1103 54-55 5.304991603970343 1103 56-57 6.275109027237129 1103 58-59 5.875491552014211 1103 60-61 5.497669008051048 1103 62-63 3.1879780956369537 1103 64-65 5.446489518177547 1103 66-67 4.496803774203226 1103 68-69 5.44110268297905 1103 70-71 4.13707713181315 1103 72-73 4.7853468516002025 1103 74-75 3.6715654938572655 1103 76-77 3.9048351046531558 1103 78-79 3.359891339480683 1103 80-81 4.4680263168075065 1103 82-83 3.8971145741905 1103 84-85 3.649675852097701 1103 86-87 3.0686613345302334 1103 88-89 3.269419239984206 1103 90-91 2.8485284779888005 1103 92-93 2.5351497852386196 1103 94-95 3.0097070641153714 1103 96-97 3.2395763656021863 1103 98-99 2.9801811493463575 1103 100 3.062291926669932 1104 1 6.225987003908141 1104 2 9.852113651628002 1104 3 7.347893967081053 1104 4 12.267879838872197 1104 5 9.5804351325793 1104 6 8.143850464146933 1104 7 4.172574383752867 1104 8 6.597645452721498 1104 9 6.555088843820176 1104 10-11 7.181958235416584 1104 12-13 7.340997266962795 1104 14-15 7.2291749108346615 1104 16-17 7.204399335601355 1104 18-19 6.722602321785029 1104 20-21 7.534406260354693 1104 22-23 6.556415052011804 1104 24-25 5.994392777111155 1104 26-27 8.244233042990778 1104 28-29 7.091806919711253 1104 30-31 6.290479339002651 1104 32-33 7.775784823355458 1104 34-35 7.955151680844608 1104 36-37 6.745532366541447 1104 38-39 7.435692069563476 1104 40-41 7.325730896606718 1104 42-43 7.210701572205586 1104 44-45 6.626477074118494 1104 46-47 6.783841508372053 1104 48-49 5.448532521453366 1104 50-51 5.650701528745664 1104 52-53 5.922532437066671 1104 54-55 4.682385595281605 1104 56-57 6.035331629112925 1104 58-59 3.8433970934177424 1104 60-61 4.3930302121075115 1104 62-63 4.110794720813676 1104 64-65 2.8368403953705297 1104 66-67 3.199279135187222 1104 68-69 4.309920788482664 1104 70-71 2.7749081523369874 1104 72-73 2.694369546009849 1104 74-75 3.1750675833832283 1104 76-77 3.8002564235074097 1104 78-79 4.388545701936415 1104 80-81 3.0997805095462816 1104 82-83 3.1394718095968273 1104 84-85 3.0919983873104755 1104 86-87 0.7426049779807791 1104 88-89 0.42950038422644354 1104 90-91 2.4496926922937625 1104 92-93 2.531948001331994 1104 94-95 2.5705012396355507 1104 96-97 2.9646488522400603 1104 98-99 2.5053009495505343 1104 100 0.8282435492864089 1105 1 5.924364650842808 1105 2 -2.6056896219051673 1105 3 0.5527722171169955 1105 4 8.847838567337796 1105 5 8.736563109041708 1105 6 8.22189240713173 1105 7 5.481827402269456 1105 8 6.153588142871929 1105 9 5.14124131263987 1105 10-11 5.154547937435677 1105 12-13 5.354071868784917 1105 14-15 6.210950220725305 1105 16-17 6.399016895729101 1105 18-19 6.3413092450194135 1105 20-21 7.187751931943204 1105 22-23 2.3948844850824322 1105 24-25 4.25520211919601 1105 26-27 7.130572976086562 1105 28-29 6.1674468161889 1105 30-31 5.527076271862569 1105 32-33 7.384097988387055 1105 34-35 6.5652325382046115 1105 36-37 6.489661956467991 1105 38-39 4.4786918096243475 1105 40-41 5.849079468605961 1105 42-43 6.023756643178881 1105 44-45 6.49894001154399 1105 46-47 5.720222025969644 1105 48-49 5.377382241221984 1105 50-51 6.310591274182382 1105 52-53 5.942014503037377 1105 54-55 5.74526554377541 1105 56-57 5.418169820899838 1105 58-59 2.2487908480791177 1105 60-61 4.70634513367601 1105 62-63 -0.6700981948888405 1105 64-65 -0.15545899642856753 1105 66-67 1.1760948843820813 1105 68-69 3.1445609888128665 1105 70-71 3.3816307838275854 1105 72-73 3.0310215300111985 1105 74-75 3.0212400151031673 1105 76-77 2.04883145065228 1105 78-79 3.661221318441914 1105 80-81 2.089709638948513 1105 82-83 1.4144713846282215 1105 84-85 1.7773202904048873 1105 86-87 -0.06756588418844345 1105 88-89 1.6754511416975681 1105 90-91 1.582939031012156 1105 92-93 1.825414764183222 1105 94-95 1.4584454292168765 1105 96-97 2.31668859530404 1105 98-99 2.2432322838315493 1105 100 2.4581068723000854 1106 1 6.215259244885772 1106 2 10.491457451427827 1106 3 6.948945096044074 1106 4 11.952972243090422 1106 5 9.781056381278109 1106 6 9.459507133671732 1106 7 6.712599073392184 1106 8 7.523221377317377 1106 9 6.914706465536089 1106 10-11 5.784180566620552 1106 12-13 8.077753479713447 1106 14-15 8.084808414706846 1106 16-17 7.8478476165548905 1106 18-19 7.283323304603531 1106 20-21 8.345151104902776 1106 22-23 6.392145895920759 1106 24-25 5.291466168936051 1106 26-27 7.82375285007199 1106 28-29 6.45160633880338 1106 30-31 5.620850522048748 1106 32-33 7.549080199400493 1106 34-35 7.065188003406682 1106 36-37 6.342922763421335 1106 38-39 7.36258052111916 1106 40-41 6.730871820553979 1106 42-43 6.440219507261602 1106 44-45 4.986491116944109 1106 46-47 6.350768650206167 1106 48-49 6.033845323679962 1106 50-51 6.849788903280423 1106 52-53 5.8919436772332645 1106 54-55 5.648220608164301 1106 56-57 5.306512012187284 1106 58-59 5.258975216984062 1106 60-61 5.059915061992182 1106 62-63 5.185644352367479 1106 64-65 5.638111210445025 1106 66-67 3.1800087451415457 1106 68-69 3.6058585729118455 1106 70-71 3.221460910225595 1106 72-73 3.0785370643161443 1106 74-75 3.1861653969632684 1106 76-77 3.771267849716395 1106 78-79 4.33433027033178 1106 80-81 3.965992142258102 1106 82-83 2.21040455801457 1106 84-85 2.994625128689407 1106 86-87 3.686590424698725 1106 88-89 3.613973174472086 1106 90-91 3.2902598210078864 1106 92-93 3.0776081459683517 1106 94-95 1.6254265906534577 1106 96-97 1.7825139841624669 1106 98-99 2.138028194952472 1106 100 2.920885066481091 1107 1 6.352913712364742 1107 2 10.709175892390071 1107 3 6.372955829303315 1107 4 11.939816220526346 1107 5 9.813354061612767 1107 6 8.387569338120063 1107 7 4.050094442598599 1107 8 6.533028869451883 1107 9 6.013431721303402 1107 10-11 4.838288991022896 1107 12-13 6.440283982904159 1107 14-15 6.606131864836701 1107 16-17 7.423446447676017 1107 18-19 6.621058078332322 1107 20-21 5.78827177548607 1107 22-23 6.042186740890795 1107 24-25 4.875971788154413 1107 26-27 7.475560249786433 1107 28-29 6.5821349319793825 1107 30-31 5.3414038310656835 1107 32-33 5.424329165744709 1107 34-35 7.094279307905481 1107 36-37 6.827437572431613 1107 38-39 6.905679078765655 1107 40-41 7.9728163283087845 1107 42-43 6.613853184924956 1107 44-45 4.508795420716854 1107 46-47 3.8157739486955684 1107 48-49 4.371531798786467 1107 50-51 5.217077865061075 1107 52-53 4.142301543917057 1107 54-55 5.72598139004851 1107 56-57 5.8841798518577875 1107 58-59 3.492838451472309 1107 60-61 3.3441519320117123 1107 62-63 0.9032890400281204 1107 64-65 2.507900866028855 1107 66-67 4.591349183332753 1107 68-69 5.250442587321022 1107 70-71 4.591713840972943 1107 72-73 2.776586348230328 1107 74-75 2.8059868382620365 1107 76-77 3.3970960731860274 1107 78-79 4.255631450880646 1107 80-81 2.376619196744432 1107 82-83 2.062697189185144 1107 84-85 2.754910842452972 1107 86-87 2.595525460963156 1107 88-89 2.862421679133682 1107 90-91 1.697558969881733 1107 92-93 2.611264322957478 1107 94-95 2.491860536282786 1107 96-97 2.567325085639961 1107 98-99 2.048030108622207 1107 100 0.3455816401768246 1108 1 4.539612886297341 1108 2 -3.22851490226936 1108 3 0.01254859725158397 1108 4 6.909167528220763 1108 5 7.020153538528064 1108 6 7.084773640425848 1108 7 4.4456853108796714 1108 8 5.22686722712405 1108 9 4.929957334733263 1108 10-11 3.3804656860805373 1108 12-13 2.671594125594204 1108 14-15 4.1855396354395715 1108 16-17 4.782773673152786 1108 18-19 4.133364388050204 1108 20-21 4.821256472380263 1108 22-23 4.270347590476899 1108 24-25 3.087617835213308 1108 26-27 4.844096595746414 1108 28-29 6.1644114930754625 1108 30-31 4.56949294029932 1108 32-33 4.697816220230614 1108 34-35 3.1770160909484346 1108 36-37 1.352916277814309 1108 38-39 2.6493663178778526 1108 40-41 5.5318706023345605 1108 42-43 3.112627313524584 1108 44-45 3.7515968507036455 1108 46-47 4.782814826911924 1108 48-49 4.965703300449455 1108 50-51 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44-45 -17.80305694231202 2213 46-47 -17.598910493360364 2213 48-49 -6.297186813870052 2213 50-51 -24.142469349714023 2213 52-53 -18.541518956950473 2213 54-55 -18.363129318001782 2213 56-57 -18.263684773991166 2213 58-59 -18.833439621136996 2213 60-61 -20.167993159026427 2213 62-63 -18.2851391788434 2213 64-65 -18.40101078154671 2213 66-67 -9.077130917744586 2213 68-69 -23.63457379635488 2213 70-71 -18.23083286556553 2213 72-73 -9.98000800498259 2213 74-75 -4.622218462191306 2213 76-77 -3.3534455821996865 2213 78-79 -4.953815643315483 2213 80-81 -3.626971708737809 2213 82-83 -3.6184162222225638 2213 84-85 -3.2334774096133927 2213 86-87 -3.499402826103001 2213 88-89 -3.0336686987731554 2213 90-91 -3.0055958970776615 2213 92-93 -3.924890028979757 2213 94-95 -3.8168538557705354 2213 96-97 -3.48814836493386 2213 98-99 -3.621402681664705 2213 100 -3.5290393028362104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 397.0 3 353.0 4 1872.0 5 6977.0 6 10917.0 7 13009.0 8 11429.0 9 11776.0 10 13483.0 11 16383.0 12 20405.0 13 28247.0 14 21432.0 15 23611.0 16 27151.0 17 29706.0 18 30063.0 19 30213.0 20 29950.0 21 29255.0 22 30272.0 23 33208.0 24 36307.0 25 40777.0 26 44522.0 27 48697.0 28 53073.0 29 56340.0 30 58525.0 31 63319.0 32 72380.0 33 88848.0 34 109058.0 35 132246.0 36 152041.0 37 142426.0 38 50136.0 39 126.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.90180696986911 22.05733241577908 14.407925570428617 23.632935043923194 2 14.246910775366983 21.789793883398335 41.48064001750245 22.482655323732228 3 17.06832688025412 27.672142990494898 30.524214604792867 24.73531552445812 4 11.436290185990432 18.215692554848815 41.23584445290372 29.112172806257032 5 12.337832220481323 38.203647557781586 36.70791050286183 12.75060971887527 6 28.72087041024371 39.61045514179029 19.586206507187434 12.082467940778567 7 24.652256541583796 33.18241134830164 25.34833065869364 16.81700145142092 8 22.643147454792793 37.60326263274635 22.629989431810113 17.123600480650744 9 23.64617904458633 17.71148787847818 23.222248519436928 35.42008455749856 10-11 22.145978873160043 28.292856906159074 30.20357156400177 19.357592656679117 12-13 22.988786570048138 25.90915760499663 30.29089861241981 20.811157212535424 14-15 20.46000540277109 27.4903440932796 27.475153095698474 24.574497408250842 16-17 19.951362643485897 31.118897415156503 28.354909082874425 20.574830858483175 18-19 19.8050270992817 30.200090478780133 30.07559686312935 19.91928555880882 20-21 21.366167600544436 29.574815009292717 28.909504825994738 20.149512564168113 22-23 20.28536210719376 29.710549416834652 29.50960589118977 20.494482584781814 24-25 20.653050932929904 29.32879475542108 29.09554546982686 20.922608841822154 26-27 20.595822233203563 29.292727712364886 29.211810714058196 20.89963934037336 28-29 20.333769569043728 29.30328752801764 29.592337558807756 20.770605344130875 30-31 20.962764870046286 29.641839029330896 28.93228929092971 20.46310680969311 32-33 20.12747943027987 29.50649760082415 29.597903079503237 20.768119889392743 34-35 20.324305004352833 28.882271788352792 29.444538582437325 21.34888462485705 36-37 20.80881376172797 29.580634868029126 29.032599332510728 20.57795203773218 38-39 20.43170698897861 29.324278672743613 29.325227419736848 20.91878691854092 40-41 20.7450920470679 29.329584924127435 28.953293739977955 20.972029288826715 42-43 20.362420205875132 29.461619595471756 29.509698382118227 20.666261816534888 44-45 20.82403770246683 29.025078994446762 29.268096986889674 20.882786316196732 46-47 20.747912671749738 29.50534520307445 29.2726217505333 20.474120374642506 48-49 20.49704421958816 29.196808956496707 29.415804736603736 20.890342087311396 50-51 20.849744543130974 29.327149471529555 29.077885083877504 20.74522090146197 52-53 20.298120488225052 29.336870751564703 29.423639500681347 20.941369259528894 54-55 20.786149129482617 29.214135457265616 29.139975498755017 20.859739914496746 56-57 20.821451862033097 29.264937060931516 29.037040107256633 20.87657096977875 58-59 20.50824124593499 28.80645424267638 29.48778162142252 21.197522889966105 60-61 20.942810827538622 29.343576652563673 28.835616574812377 20.877995945085324 62-63 20.418013783110936 29.4380653241946 29.369501799134532 20.77441909355993 64-65 20.475190009067955 28.887772184264254 29.37410481860266 21.262932988065135 66-67 20.613278120681414 29.196496019761693 29.095892824802622 21.094333034754275 68-69 21.018249574169896 29.093309696786164 29.050785375255337 20.837655353788605 70-71 21.16692272118015 29.17698723124074 28.784903757469344 20.871186290109765 72-73 20.688393430530596 29.32339900322391 29.33109886394702 20.657108702298473 74-75 20.980124515454516 28.86857805485018 28.925818781836337 21.22547864785897 76-77 20.966349218209768 29.38617864052529 28.94103794018476 20.706434201080175 78-79 20.951418021498526 29.109853011133204 29.08543539400079 20.85329357336748 80-81 21.16166094416689 29.041556155365544 28.870401981195826 20.92638091927174 82-83 20.56985411603593 29.273496381565106 29.383175120815224 20.773474381583735 84-85 20.796216951715746 28.849388406333816 28.943519442672006 21.410875199278436 86-87 21.028108482450737 29.217017113786646 28.958133733350117 20.7967406704125 88-89 20.805091708258878 28.783222420769405 29.21361836835295 21.19806750261877 90-91 21.11775844711297 28.831296878451795 28.921293275398885 21.12965139903635 92-93 20.695902702166883 29.145254460310078 29.26244801834167 20.89639481918137 94-95 20.697981141861472 28.713506926279752 29.063721261168844 21.524790670689924 96-97 20.864794931840457 28.970665460267153 29.063356159445945 21.101183448446445 98-99 20.813269847245405 28.808348971168122 29.103371049806103 21.27501013178037 100 21.00940124214696 28.6205280724957 28.79221628874348 21.577854396613862 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 191.0 1 207.5 2 195.5 3 160.0 4 206.0 5 316.5 6 465.0 7 679.0 8 976.0 9 1399.5 10 1917.0 11 2464.5 12 3002.5 13 3576.5 14 4126.0 15 4639.5 16 5128.5 17 5639.5 18 6211.5 19 6744.0 20 7323.0 21 8262.5 22 9502.5 23 11004.5 24 13026.5 25 15523.0 26 18531.0 27 22235.0 28 26637.0 29 31638.0 30 36707.5 31 41545.5 32 46305.0 33 50892.5 34 55083.0 35 58369.0 36 60825.0 37 62576.0 38 63726.0 39 63754.5 40 63183.0 41 61751.0 42 59139.0 43 56487.5 44 53149.0 45 50004.5 46 47141.0 47 44223.0 48 41629.5 49 39378.0 50 37777.0 51 35046.5 52 31085.5 53 27534.5 54 24866.5 55 22709.5 56 20576.5 57 18309.5 58 15852.0 59 13684.0 60 11679.5 61 9523.0 62 7521.5 63 5970.0 64 4712.0 65 3669.0 66 2794.5 67 2094.0 68 1587.0 69 1167.0 70 840.0 71 617.5 72 462.5 73 320.5 74 226.0 75 178.5 76 123.5 77 77.5 78 61.5 79 44.0 80 31.5 81 22.5 82 13.0 83 7.5 84 8.5 85 6.0 86 3.0 87 3.0 88 4.5 89 4.0 90 2.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 1.0 97 1.0 98 0.5 99 1.5 100 3.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.658149184476044 2 24.25379080009943 3 5.772469135015584 4 23.983734137278272 5 12.294111273288165 6 9.684498352380285 7 7.736674038994728 8 8.448050582243951 9 5.2046936447132754 10-11 6.714225618733787 12-13 9.86516288170919 14-15 7.273810813739299 16-17 7.690623545983569 18-19 6.590032697443481 20-21 7.74795561306113 22-23 5.682917657256857 24-25 5.206095874258253 26-27 9.4547557889772 28-29 6.17404855538488 30-31 4.283365095957118 32-33 8.617879701452583 34-35 7.0913934974791735 36-37 6.917580771608677 38-39 5.946696155978916 40-41 8.439477860707616 42-43 6.273511246518328 44-45 6.262675836398055 46-47 5.717686576201615 48-49 3.8095071163149403 50-51 6.3359104612697825 52-53 4.395734672675008 54-55 4.8145232738235615 56-57 4.657855991025731 58-59 3.443079041129942 60-61 4.022040498938767 62-63 3.1340786395823903 64-65 4.301562211188517 66-67 2.337421044915962 68-69 4.972720261579547 70-71 2.422415276653515 72-73 1.4944898752653082 74-75 0.8425806122644095 76-77 1.0516402898790895 78-79 1.7215873238449135 80-81 2.0962056943267067 82-83 0.7718954956562752 84-85 1.208658130063164 86-87 0.5096148330390775 88-89 0.6976729363324049 90-91 0.31731817225752584 92-93 0.33637574652788843 94-95 0.5208326693988897 96-97 0.5330703090641393 98-99 0.4469925363145584 100 0.43316145398456274 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1568930.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.71094631727107 #Duplication Level Percentage of deduplicated Percentage of total 1 98.51904271862514 96.26388894308515 2 1.2466623073142975 2.4362510757150524 3 0.13581481445608692 0.39811782133226514 4 0.04374711942656555 0.17098289751297568 5 0.016438501890586342 0.08031107878837206 6 0.009952296563761525 0.058346898916515816 7 0.006509998820425168 0.044526870168764304 8 0.005554825366494076 0.04342137945498546 9 0.004070670711820446 0.03579741786581776 >10 0.010097756285165384 0.15669712749892278 >50 0.0011938814192884693 0.07874545091197761 >100 7.84395567350825E-4 0.13661106574916282 >500 1.3071355300093635E-4 0.09630197300001551 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 1.2747541317968298E-4 0.0 0.0 0.0 14-15 3.1868853294920745E-5 1.2747541317968298E-4 0.0 0.0 0.0 16-17 6.373770658984149E-5 1.2747541317968298E-4 0.0 0.0 0.0 18-19 6.373770658984149E-5 1.2747541317968298E-4 0.0 6.373770658984149E-5 0.0 20-21 6.373770658984149E-5 1.2747541317968298E-4 0.0 6.373770658984149E-5 0.0 22-23 6.373770658984149E-5 1.2747541317968298E-4 0.0 1.9121311976952446E-4 0.0 24-25 6.373770658984149E-5 1.2747541317968298E-4 0.0 2.5495082635936596E-4 0.0 26-27 6.373770658984149E-5 1.2747541317968298E-4 0.0 2.5495082635936596E-4 0.0 28-29 6.373770658984149E-5 1.2747541317968298E-4 0.0 4.7803279942381115E-4 0.0 30-31 6.373770658984149E-5 1.2747541317968298E-4 0.0 0.00172091807792572 0.0 32-33 9.560655988476223E-5 1.2747541317968298E-4 0.0 0.004525377167878745 0.0 34-35 1.2747541317968298E-4 1.2747541317968298E-4 0.0 0.010516721587323846 0.0 36-37 1.2747541317968298E-4 1.2747541317968298E-4 0.0 0.019663082482966096 0.0 38-39 1.2747541317968298E-4 1.2747541317968298E-4 0.0 0.03413154187886011 0.0 40-41 1.2747541317968298E-4 1.2747541317968298E-4 0.0 0.05127698495152748 0.0 42-43 1.2747541317968298E-4 1.2747541317968298E-4 0.0 0.06918728050327294 0.0 44-45 1.2747541317968298E-4 1.2747541317968298E-4 0.0 0.09146360895642253 3.1868853294920745E-5 46-47 1.2747541317968298E-4 1.2747541317968298E-4 0.0 0.1180103637510915 6.373770658984149E-5 48-49 1.2747541317968298E-4 1.2747541317968298E-4 0.0 0.14876380718069004 6.373770658984149E-5 50-51 1.2747541317968298E-4 1.9121311976952446E-4 0.0 0.1791666932240444 6.373770658984149E-5 52-53 1.2747541317968298E-4 1.9121311976952446E-4 0.0 0.21116302193214484 6.373770658984149E-5 54-55 1.2747541317968298E-4 1.9121311976952446E-4 0.0 0.2500111540986532 6.373770658984149E-5 56-57 1.2747541317968298E-4 1.9121311976952446E-4 0.0 0.2988342373464718 6.373770658984149E-5 58-59 1.2747541317968298E-4 1.9121311976952446E-4 0.0 0.35393548469338976 6.373770658984149E-5 60-61 1.2747541317968298E-4 2.5495082635936596E-4 0.0 0.412128010809915 6.373770658984149E-5 62-63 1.2747541317968298E-4 2.5495082635936596E-4 0.0 0.47159529105823716 6.373770658984149E-5 64-65 1.5934426647460373E-4 2.5495082635936596E-4 0.0 0.5385836206841605 6.373770658984149E-5 66-67 1.9121311976952446E-4 2.5495082635936596E-4 0.0 0.6211558195713002 6.373770658984149E-5 68-69 1.9121311976952446E-4 2.5495082635936596E-4 0.0 0.7153920187643807 6.373770658984149E-5 70-71 1.9121311976952446E-4 2.5495082635936596E-4 0.0 0.8237142511138165 6.373770658984149E-5 72-73 1.9121311976952446E-4 2.5495082635936596E-4 0.0 0.9386014672420058 6.373770658984149E-5 74-75 1.9121311976952446E-4 2.5495082635936596E-4 0.0 1.057057994939226 6.373770658984149E-5 76-77 2.230819730644452E-4 2.5495082635936596E-4 0.0 1.1867960967028484 6.373770658984149E-5 78-79 2.5495082635936596E-4 2.5495082635936596E-4 0.0 1.341455641743099 6.373770658984149E-5 80-81 2.5495082635936596E-4 2.5495082635936596E-4 0.0 1.5171804988112918 6.373770658984149E-5 82-83 2.5495082635936596E-4 2.5495082635936596E-4 0.0 1.709636503859318 6.373770658984149E-5 84-85 2.5495082635936596E-4 2.5495082635936596E-4 0.0 1.9004034596827135 6.373770658984149E-5 86-87 2.5495082635936596E-4 2.5495082635936596E-4 0.0 2.111279661935204 6.373770658984149E-5 88 2.5495082635936596E-4 2.5495082635936596E-4 0.0 2.30010261770761 6.373770658984149E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGTC 60 0.003016225 33.461346 8 TATAGCG 70 0.00651147 28.591135 5 GTACCGT 75 0.00900575 26.75284 6 AACCGTC 115 0.002800805 21.691786 7 GTTAGAC 285 1.4901889E-7 19.692373 3 GTACATG 4460 0.0 19.547153 1 TACATGG 4530 0.0 18.283688 2 CGCGTCC 70 0.009615787 17.524536 22-23 ACATGGG 4665 0.0 16.361765 3 GTATTAT 1025 0.0 16.286926 1 GTATAGG 430 2.300767E-7 15.816976 1 GTCCTGG 995 0.0 15.535176 1 TTAGACT 860 0.0 15.010345 4 CACACCG 200 0.0037371388 15.010345 5 GTGTAGG 495 6.931077E-8 14.989091 1 GTGTATA 955 0.0 14.890994 1 GTCTTAG 545 1.4077159E-8 14.748441 1 CTAATAC 650 1.2732926E-11 14.6783905 3 CATGGGG 1685 0.0 14.655954 4 CTAGGAC 460 9.2470145E-8 14.640851 3 >>END_MODULE