FastQCFastQC Report
Wed 25 May 2016
SRR1295103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568930
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20850.13289311823981947No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16440.1047847896336994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG804.7651588E-429.3923555
GTATCAA47600.021.0573391
CGTACAC1456.576582E-419.4591433
CTACACC5900.019.1299384
CAACGCA50750.018.8111065
TCAACGC51300.018.70114
GTCTCGC2402.0369462E-618.6075971
GAGTACT22100.018.58931512-13
AACGCAG51200.018.5267096
TATCAAC53950.018.3849952
ATCAACG52350.018.325423
AGACGAT1300.00808689818.0587677
GTACATG57050.016.9604761
GTACTAG2957.800463E-716.8204271
CTAAGAC7000.016.7950943
ACGCAGA56600.016.7570047
CGCAGAG57100.016.5280428
CCTACAC7700.016.489733
TACATGG58500.016.1920742
ACACCGT1750.002342797816.1001536
Produced by FastQC (version 0.11.5)