FastQCFastQC Report
Wed 25 May 2016
SRR1295102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575935
Sequences flagged as poor quality0
Sequence length100
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32100.1246149456410973No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27360.10621386020998201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG3757.6397555E-1118.766869
TCTATAC9700.016.77083
GTCTTAC7150.016.4864731
TATACCG2153.2382697E-416.2814855
GTACTAT7500.015.7171061
CTATACT10450.015.5687674
TACGACT2000.0036289915.0773648
ACCGATC2050.00427656314.7096228
CCGATCC3102.0513222E-514.5940429
ATACGAC2407.6131435E-414.5489097
GTATAAT13650.014.5444581
CTGTCGC6251.8189894E-1214.4772919
TCTGTCG6303.6379788E-1214.3593948
GTATAGG6506.148184E-1014.3172021
GTATAAC7552.7284841E-1113.9695431
GTGTATA15200.013.8776371
CACGTAC2850.001149204713.7778263
GTATATA21650.013.4685291
GTACATG140350.013.3055941
TAATACA21950.013.29054
Produced by FastQC (version 0.11.5)