##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295102_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2575935 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.29597214215421 33.0 27.0 33.0 6.0 33.0 2 22.50011005712489 27.0 6.0 33.0 2.0 33.0 3 24.873464974853793 27.0 15.0 33.0 15.0 33.0 4 25.013088063169295 33.0 6.0 37.0 2.0 37.0 5 27.029754632783824 33.0 15.0 37.0 2.0 37.0 6 27.743756344783545 33.0 15.0 37.0 6.0 37.0 7 27.666345618193006 33.0 15.0 37.0 6.0 37.0 8 28.277770207710986 33.0 22.0 37.0 6.0 37.0 9 29.353642463804405 33.0 27.0 37.0 15.0 37.0 10-11 28.86371725218222 35.0 24.5 37.0 10.5 37.0 12-13 27.68765574441902 33.0 15.0 37.0 4.0 37.0 14-15 29.45493481007867 33.0 24.5 37.0 10.5 40.0 16-17 29.864540836628255 35.0 27.0 40.0 6.0 40.0 18-19 30.037413017021002 37.0 27.0 40.0 10.5 40.0 20-21 29.57704270488192 33.0 24.5 40.0 6.0 40.0 22-23 30.33163686195498 35.0 27.0 40.0 15.0 40.0 24-25 30.482199861409548 37.0 27.0 40.0 15.0 40.0 26-27 29.048505105913 33.0 22.0 40.0 4.0 40.0 28-29 29.747834475637 33.0 27.0 40.0 10.5 40.0 30-31 30.427252240448613 33.0 27.0 40.0 15.0 40.0 32-33 29.23352704940148 33.0 24.5 38.5 6.0 40.0 34-35 28.847310976402746 33.0 22.0 37.0 6.0 40.0 36-37 29.208742456622545 33.0 22.0 37.0 6.0 40.0 38-39 29.285347650464782 33.0 24.5 37.0 6.0 40.0 40-41 28.326058887355465 33.0 22.0 37.0 6.0 40.0 42-43 28.99260909145611 33.0 22.0 37.0 6.0 40.0 44-45 28.885800689846597 33.0 22.0 37.0 6.0 40.0 46-47 28.607874616401425 33.0 22.0 37.0 6.0 40.0 48-49 28.560349737085758 33.0 22.0 37.0 6.0 40.0 50-51 28.562288450601432 33.0 22.0 37.0 6.0 40.0 52-53 28.73833714748237 33.0 22.0 37.0 6.0 40.0 54-55 28.724647555159585 33.0 22.0 37.0 6.0 40.0 56-57 28.722288994093407 33.0 22.0 37.0 6.0 40.0 58-59 28.60389800208468 33.0 22.0 37.0 6.0 40.0 60-61 28.560251132113194 33.0 22.0 37.0 10.5 40.0 62-63 28.50426214170777 33.0 22.0 37.0 6.0 40.0 64-65 28.079518893139774 33.0 22.0 37.0 6.0 40.0 66-67 28.288997004971012 33.0 22.0 37.0 10.5 40.0 68-69 27.885965096168967 33.0 22.0 37.0 6.0 40.0 70-71 27.996308136657174 33.0 22.0 37.0 10.5 37.0 72-73 28.044984248437945 33.0 22.0 37.0 10.5 37.0 74-75 28.112497597959575 33.0 22.0 37.0 15.0 37.0 76-77 27.859336124552833 33.0 22.0 37.0 10.5 37.0 78-79 27.432014394773162 33.0 22.0 37.0 10.5 37.0 80-81 27.237805495868493 33.0 22.0 37.0 6.0 37.0 82-83 27.357117512670158 30.0 22.0 33.0 15.0 37.0 84-85 27.315680519888893 30.0 22.0 33.0 10.5 37.0 86-87 27.07715547946668 27.0 22.0 33.0 15.0 37.0 88-89 27.012119482828567 27.0 22.0 33.0 15.0 37.0 90-91 27.163863800911123 27.0 22.0 33.0 15.0 37.0 92-93 27.08914161265715 27.0 22.0 33.0 15.0 37.0 94-95 26.968334604716347 27.0 22.0 33.0 15.0 37.0 96-97 27.051511781159075 27.0 22.0 33.0 15.0 37.0 98-99 27.105891453006386 27.0 22.0 33.0 15.0 37.0 100 26.77099849180977 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.3337006248135275 1101 2 11.382873977853787 1101 3 8.452182206118739 1101 4 12.97997086724191 1101 5 10.903662651133782 1101 6 10.10787291417881 1101 7 9.596814947596492 1101 8 9.094016326018696 1101 9 7.34015468957082 1101 10-11 7.910816405409822 1101 12-13 9.284486590386607 1101 14-15 8.127518454889323 1101 16-17 8.33230556373432 1101 18-19 8.406240461173724 1101 20-21 7.56537182374727 1101 22-23 7.575251612132007 1101 24-25 7.5305175977584895 1101 26-27 8.868932497108997 1101 28-29 8.450764157074385 1101 30-31 5.703297466198471 1101 32-33 7.252348447443868 1101 34-35 8.941415376962759 1101 36-37 8.334430856243774 1101 38-39 8.370299798891192 1101 40-41 9.110239314381595 1101 42-43 8.789602436610455 1101 44-45 8.760285768555288 1101 46-47 7.585007476366968 1101 48-49 5.820609787838677 1101 50-51 7.069826592459272 1101 52-53 6.148203541448918 1101 54-55 4.210673293966163 1101 56-57 6.387033620036618 1101 58-59 6.45376490089863 1101 60-61 6.940682066560509 1101 62-63 6.5628206463978245 1101 64-65 6.985968775198611 1101 66-67 6.010813467740924 1101 68-69 6.918340070025845 1101 70-71 6.4453791276317425 1101 72-73 5.604573826707995 1101 74-75 5.080138737861244 1101 76-77 5.250067725900738 1101 78-79 5.389281893570136 1101 80-81 5.311328385553882 1101 82-83 4.667265293029441 1101 84-85 4.499110638947549 1101 86-87 4.396897380894103 1101 88-89 4.379778367507701 1101 90-91 4.249301699548269 1101 92-93 4.003273447892454 1101 94-95 3.8960378483747107 1101 96-97 4.0305476627213395 1101 98-99 3.6993878730988925 1101 100 3.7617437186223732 1102 1 6.802890740958738 1102 2 10.453798969118967 1102 3 8.078185557060081 1102 4 12.588815730342617 1102 5 10.511324710352195 1102 6 9.372813969052682 1102 7 6.791153305804357 1102 8 7.772816990364539 1102 9 6.483508521378784 1102 10-11 7.406560223676404 1102 12-13 8.943546950764627 1102 14-15 6.247624170208443 1102 16-17 7.246493665778512 1102 18-19 7.4247716318853065 1102 20-21 7.0210661178067895 1102 22-23 6.609537645994266 1102 24-25 6.870113718615308 1102 26-27 8.49741527750626 1102 28-29 7.666578299753457 1102 30-31 4.162163344752024 1102 32-33 4.44571204226056 1102 34-35 5.084965285732444 1102 36-37 5.189155460601686 1102 38-39 4.625127491396391 1102 40-41 6.331830832786977 1102 42-43 6.927701492327646 1102 44-45 6.505657227975188 1102 46-47 5.576255554034393 1102 48-49 3.9726441932195584 1102 50-51 5.221365928736116 1102 52-53 5.919944109094846 1102 54-55 5.5071504045153254 1102 56-57 6.257025609757008 1102 58-59 6.04306789126208 1102 60-61 6.009163086541232 1102 62-63 4.468005784177329 1102 64-65 6.280408578370203 1102 66-67 4.110326489634279 1102 68-69 6.121415456212414 1102 70-71 5.378545999744407 1102 72-73 5.153591145286114 1102 74-75 4.312904865371518 1102 76-77 3.4554057085787058 1102 78-79 4.533863212321993 1102 80-81 4.55847280159335 1102 82-83 4.010693442858567 1102 84-85 3.097655479793211 1102 86-87 3.2371382350881817 1102 88-89 3.429723604331766 1102 90-91 3.40824405820662 1102 92-93 3.26312245950313 1102 94-95 3.2023042489801767 1102 96-97 3.3598784027074338 1102 98-99 3.1365260604156653 1102 100 3.336671831744127 1103 1 6.664318346627699 1103 2 10.838096343415891 1103 3 7.90952319880634 1103 4 12.094394273312275 1103 5 10.379552594477989 1103 6 9.116448349609175 1103 7 8.162229462552638 1103 8 8.032040742983504 1103 9 7.114095911275488 1103 10-11 7.518178699160959 1103 12-13 8.718732019396349 1103 14-15 8.508026421087255 1103 16-17 7.861452841467422 1103 18-19 7.3280001650560465 1103 20-21 8.355687907539966 1103 22-23 6.929582002913335 1103 24-25 5.223020687476783 1103 26-27 6.077162775342291 1103 28-29 7.130170478440679 1103 30-31 6.13678536283485 1103 32-33 8.040271047517596 1103 34-35 7.153403296944539 1103 36-37 4.275045645050465 1103 38-39 6.469787567321351 1103 40-41 6.8173394689771385 1103 42-43 7.572522833704074 1103 44-45 5.633354227065102 1103 46-47 6.38997627995014 1103 48-49 5.58276800024672 1103 50-51 6.467523994567891 1103 52-53 6.102268052651741 1103 54-55 5.0248943560553805 1103 56-57 5.8772745342269275 1103 58-59 5.607897410410271 1103 60-61 5.213190545067395 1103 62-63 2.888269103757139 1103 64-65 5.070756971816905 1103 66-67 4.148683456831787 1103 68-69 5.180399451432123 1103 70-71 3.7978508184344584 1103 72-73 4.536426416899264 1103 74-75 3.4118802955332797 1103 76-77 3.702995922421014 1103 78-79 3.0401392758880483 1103 80-81 4.18737786438399 1103 82-83 3.4719831013376705 1103 84-85 3.3731730825993687 1103 86-87 2.9685575637311388 1103 88-89 3.140755380158325 1103 90-91 2.779743002381423 1103 92-93 2.4439805864508735 1103 94-95 2.8986399896507464 1103 96-97 3.010895642003007 1103 98-99 2.7450876185179673 1103 100 2.9289454592346225 1104 1 6.421158330004516 1104 2 9.815866603909608 1104 3 7.411570968650594 1104 4 12.337509032068027 1104 5 9.743268404141237 1104 6 8.148309491864897 1104 7 4.087304699627882 1104 8 6.654036264767072 1104 9 6.644883748339243 1104 10-11 7.321636862722233 1104 12-13 7.62158584177012 1104 14-15 7.362517058654976 1104 16-17 7.346652230453177 1104 18-19 7.044123561127137 1104 20-21 7.64678376898879 1104 22-23 6.609612613088487 1104 24-25 6.203794748970665 1104 26-27 8.256319314047015 1104 28-29 7.361928518649496 1104 30-31 6.487984200797811 1104 32-33 7.736693528719062 1104 34-35 8.140404444578344 1104 36-37 6.760974445505557 1104 38-39 7.411610197444329 1104 40-41 7.320020882000442 1104 42-43 7.475505591447632 1104 44-45 6.917081678241114 1104 46-47 6.831456800237227 1104 48-49 5.676156915967319 1104 50-51 5.890001297912548 1104 52-53 6.172654475555792 1104 54-55 5.039507229507112 1104 56-57 6.203152949791935 1104 58-59 4.112312530105765 1104 60-61 4.517702952660407 1104 62-63 3.9642603336413345 1104 64-65 2.987217654622679 1104 66-67 3.5361603406327085 1104 68-69 4.380564427921154 1104 70-71 3.010517537516563 1104 72-73 2.6892013518369495 1104 74-75 3.421253761473263 1104 76-77 4.120920746790755 1104 78-79 4.567446419561623 1104 80-81 3.342873821237717 1104 82-83 3.255586836664378 1104 84-85 3.1536372763585376 1104 86-87 0.9433743684728384 1104 88-89 0.5864251932928859 1104 90-91 2.4310904975731873 1104 92-93 2.633080245596364 1104 94-95 2.685814901067552 1104 96-97 3.0724539727871445 1104 98-99 2.708865335658281 1104 100 0.9685848101905528 1105 1 5.800576688166181 1105 2 -2.706140706053233 1105 3 0.5038330004596716 1105 4 8.629233488626507 1105 5 8.758465808932083 1105 6 8.144064171951275 1105 7 5.372574673763285 1105 8 6.155340232748536 1105 9 5.133180648962721 1105 10-11 5.033448173146422 1105 12-13 5.2380762774380685 1105 14-15 5.98126945321798 1105 16-17 6.230543135157188 1105 18-19 6.28168747690215 1105 20-21 7.117999530880731 1105 22-23 2.1730011865321437 1105 24-25 4.119296901260235 1105 26-27 7.01204468932227 1105 28-29 5.959762507988135 1105 30-31 5.3905245918556375 1105 32-33 7.155075586299933 1105 34-35 6.173209692532446 1105 36-37 6.324633065998643 1105 38-39 4.255915015205655 1105 40-41 5.55424407582051 1105 42-43 5.698703156649341 1105 44-45 6.270058260906399 1105 46-47 5.484689874571089 1105 48-49 5.15916244550214 1105 50-51 6.026576963451923 1105 52-53 5.683067792043776 1105 54-55 5.344217641497892 1105 56-57 5.20500576155575 1105 58-59 2.0990728338523574 1105 60-61 4.406875086774495 1105 62-63 -0.898002944334781 1105 64-65 -0.4067920933201492 1105 66-67 0.9643786333043138 1105 68-69 2.988872086678196 1105 70-71 3.1093796809503935 1105 72-73 2.8124904241451283 1105 74-75 2.7787984412017046 1105 76-77 1.7843923096565675 1105 78-79 3.3110972316250518 1105 80-81 1.6570035326439907 1105 82-83 1.0219248157261163 1105 84-85 1.4642391418971172 1105 86-87 -0.23956295330207666 1105 88-89 1.708667617856694 1105 90-91 1.4308791131744023 1105 92-93 1.8111928792113225 1105 94-95 1.1014512483133245 1105 96-97 2.025931541117533 1105 98-99 1.9280484201338197 1105 100 1.9328457337063902 1106 1 6.128801306407599 1106 2 10.249243813809112 1106 3 6.8495678988320385 1106 4 11.697453579837784 1106 5 9.927158461310658 1106 6 9.302275885013014 1106 7 6.335915553893397 1106 8 7.327418071468891 1106 9 6.78914666165517 1106 10-11 5.579911470144747 1106 12-13 7.8198338392550895 1106 14-15 7.601005536268023 1106 16-17 7.6195332432819605 1106 18-19 7.099113700457799 1106 20-21 8.051820074480645 1106 22-23 6.133676410892598 1106 24-25 4.956645856165984 1106 26-27 7.697319554454499 1106 28-29 6.251246071829744 1106 30-31 5.264452238491586 1106 32-33 7.247184144901926 1106 34-35 6.697473382082968 1106 36-37 5.882091963813512 1106 38-39 6.910884648998259 1106 40-41 6.395198215538073 1106 42-43 6.013986572838505 1106 44-45 4.4123350505504 1106 46-47 5.954334015420407 1106 48-49 5.772538155994376 1106 50-51 6.503064373173295 1106 52-53 5.518238441169597 1106 54-55 5.1432733051060175 1106 56-57 4.748706666333025 1106 58-59 4.8488591229241855 1106 60-61 4.8673057519545075 1106 62-63 4.903012532973477 1106 64-65 5.150199823520822 1106 66-67 3.1929618248186493 1106 68-69 3.37836559112349 1106 70-71 2.9312826343135967 1106 72-73 2.7703544623398493 1106 74-75 2.739173959399949 1106 76-77 3.416016104020418 1106 78-79 4.097037475875094 1106 80-81 3.6844986257415258 1106 82-83 2.1601558996564165 1106 84-85 2.6303920858290084 1106 86-87 3.1468550262382635 1106 88-89 3.2522256775422314 1106 90-91 3.103338723577348 1106 92-93 2.8242281979058603 1106 94-95 1.54671708296355 1106 96-97 1.5710686907442621 1106 98-99 1.998746261306323 1106 100 2.8937111562082443 1107 1 6.169237736282586 1107 2 10.481104375447366 1107 3 6.222518674266247 1107 4 11.412199479940668 1107 5 9.60504234257819 1107 6 8.104938455292828 1107 7 3.841617413471724 1107 8 6.511417672088946 1107 9 5.891410273218813 1107 10-11 4.7319364048012424 1107 12-13 6.386842297843309 1107 14-15 6.272422743383256 1107 16-17 7.136185060447556 1107 18-19 6.467433218500716 1107 20-21 5.698378249290592 1107 22-23 5.921657160884436 1107 24-25 4.7606685264408455 1107 26-27 7.352262355189968 1107 28-29 6.577624311098607 1107 30-31 5.17004533153527 1107 32-33 5.202122858377219 1107 34-35 6.732630878499869 1107 36-37 6.63113246138774 1107 38-39 6.559502908244024 1107 40-41 7.810993054032714 1107 42-43 6.29469930545034 1107 44-45 4.24004918546763 1107 46-47 3.776403765798726 1107 48-49 4.26508343112161 1107 50-51 5.002656157240644 1107 52-53 3.956062624744309 1107 54-55 5.393990385614909 1107 56-57 5.452318007512492 1107 58-59 3.2329309482616715 1107 60-61 3.2365845749710083 1107 62-63 0.7659897189534277 1107 64-65 2.445296667892695 1107 66-67 4.362873014776078 1107 68-69 5.108498857524921 1107 70-71 4.470875597828648 1107 72-73 2.5988553948406867 1107 74-75 2.7067976263344455 1107 76-77 3.2830116229089548 1107 78-79 3.9295563299258127 1107 80-81 2.31843779612117 1107 82-83 1.8396176871304704 1107 84-85 2.6135722368562355 1107 86-87 2.637352985504144 1107 88-89 2.8109612023227406 1107 90-91 1.501535126235531 1107 92-93 2.432568219383395 1107 94-95 2.4784846347513074 1107 96-97 2.5058573925567167 1107 98-99 2.079079138700628 1107 100 0.3113074167798331 1108 1 4.393211960211552 1108 2 -3.3132696877403607 1108 3 -0.08600147672680336 1108 4 6.991610122177086 1108 5 7.243916597467408 1108 6 6.921104705569459 1108 7 4.545065013261567 1108 8 5.217145606962752 1108 9 4.9422589274599495 1108 10-11 3.427783279505203 1108 12-13 2.606031299124723 1108 14-15 4.065694208668759 1108 16-17 4.7792687314150974 1108 18-19 4.184632531007331 1108 20-21 4.779414868309068 1108 22-23 4.225692319797481 1108 24-25 3.0119346526903144 1108 26-27 4.8130434719216275 1108 28-29 6.191996237992694 1108 30-31 4.6904573527619675 1108 32-33 4.675706682970464 1108 34-35 3.154679476339197 1108 36-37 1.5282163683207095 1108 38-39 2.7599923519916274 1108 40-41 5.615750559070335 1108 42-43 3.3405768481483733 1108 44-45 3.830137050748295 1108 46-47 4.900712886900681 1108 48-49 4.973657155996445 1108 50-51 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2212 20-21 -24.000474000663864 2212 22-23 -19.072316367977933 2212 24-25 -25.602512380828237 2212 26-27 -17.540525657713932 2212 28-29 -18.439400125644642 2212 30-31 -6.6121665572121024 2212 32-33 -17.68257770093541 2212 34-35 -14.151554129979145 2212 36-37 -18.064265984593774 2212 38-39 -24.07349035100174 2212 40-41 -10.576016394363837 2212 42-43 -14.314931382175505 2212 44-45 -17.551300453497564 2212 46-47 -17.545622809520733 2212 48-49 -18.29837015309278 2212 50-51 -9.415643245328116 2212 52-53 -2.6676086669353722 2212 54-55 -24.052174671772594 2212 56-57 -23.99858379609472 2212 58-59 -13.058258077050848 2212 60-61 -6.6977782954934035 2212 62-63 -3.6349419080588063 2212 64-65 -17.843976049341677 2212 66-67 -18.215822994992056 2212 68-69 -15.175461400363089 2212 70-71 -10.209494890660686 2212 72-73 -18.077512232446647 2212 74-75 -6.803442782731164 2212 76-77 -11.406210658382797 2212 78-79 -5.055553807766479 2212 80-81 -2.9266212653443446 2212 82-83 -3.270886133751663 2212 84-85 -5.43036685638474 2212 86-87 -5.120037921674054 2212 88-89 -3.697026613829429 2212 90-91 -4.889281367647115 2212 92-93 -5.963616758709257 2212 94-95 -4.386847328844198 2212 96-97 -5.854156075115107 2212 98-99 -4.202993908768441 2212 100 -4.121571066104842 2213 1 -21.146848982609743 2213 2 -17.105705608156207 2213 3 -20.046747130069697 2213 4 -19.137445264092854 2213 5 -21.34740801267778 2213 6 -22.012933652559237 2213 7 -10.870231557024853 2213 8 -10.523098523148125 2213 9 -3.9790436572191084 2213 10-11 -17.803575429236822 2213 12-13 -16.396491990271077 2213 14-15 -23.875782758529663 2213 16-17 -18.217934501536746 2213 18-19 -18.702349518060053 2213 20-21 -17.946413566291817 2213 22-23 -20.12505705043803 2213 24-25 -7.523192267513792 2213 26-27 -23.578872642077297 2213 28-29 -24.48258702065581 2213 30-31 -19.789848617234448 2213 32-33 -23.724275392670332 2213 34-35 -18.164189330722763 2213 36-37 -24.17074401884545 2213 38-39 -11.801063533249145 2213 40-41 -17.498734091906513 2213 42-43 -23.705847242190398 2213 44-45 -17.56486517583287 2213 46-47 -17.414695791028034 2213 48-49 -6.335990465319224 2213 50-51 -23.906708025670632 2213 52-53 -18.271441572421587 2213 54-55 -18.151088741649836 2213 56-57 -18.092068215358175 2213 58-59 -18.475002086663874 2213 60-61 -19.96857968091651 2213 62-63 -18.05286311247129 2213 64-65 -18.148993251116064 2213 66-67 -8.997245324744412 2213 68-69 -23.34932588286495 2213 70-71 -17.97973835219347 2213 72-73 -9.756692812054688 2213 74-75 -4.4938379137817535 2213 76-77 -3.31625378059098 2213 78-79 -4.858466578282222 2213 80-81 -3.5854708452666806 2213 82-83 -3.578600670780304 2213 84-85 -3.5695551562250607 2213 86-87 -3.577484535705331 2213 88-89 -3.021689978655406 2213 90-91 -3.0127303779034875 2213 92-93 -3.859242804157379 2213 94-95 -3.85923484231769 2213 96-97 -3.5212291438090055 2213 98-99 -3.7965804558769705 2213 100 -3.3899735897348684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 709.0 3 637.0 4 3422.0 5 12576.0 6 19620.0 7 22380.0 8 20423.0 9 20818.0 10 23717.0 11 28923.0 12 35830.0 13 48993.0 14 38310.0 15 41882.0 16 47505.0 17 51215.0 18 51009.0 19 51664.0 20 52008.0 21 50411.0 22 51748.0 23 56294.0 24 61553.0 25 67679.0 26 74257.0 27 79859.0 28 86275.0 29 90905.0 30 95503.0 31 104200.0 32 118001.0 33 143270.0 34 174936.0 35 209540.0 36 238286.0 37 222339.0 38 78997.0 39 241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.6659401312911 21.543208392032078 14.247814381603213 23.54303709507361 2 14.742606982643332 21.824778637059566 40.627182977565944 22.805431402731156 3 17.81289768126002 27.34894309749285 29.99062091989357 24.847538301353563 4 11.858394106708275 18.125962344769047 39.794173547025984 30.221470001496698 5 12.656795442797767 38.00313502375608 36.41606243848556 12.924007094960595 6 29.490191689302335 38.736090364739155 18.846162124466755 12.92755582149176 7 25.008158482147557 32.27697657389227 25.25461833251994 17.460246611440233 8 23.12181685076532 36.83763133451755 22.94111810066577 17.09943371405136 9 23.80107225819277 17.54026081226401 22.887521694473907 35.77114523506931 10-11 22.511598774056864 28.13128325570931 29.65437102440564 19.702746945828185 12-13 23.368985469026537 25.789397242885205 29.77952879272694 21.062088495361323 14-15 21.045750839002704 27.190419378671393 26.926644435864922 24.837185346460984 16-17 20.34786960221454 30.935457783727514 28.04384318312827 20.672829430929674 18-19 20.358138614124922 29.906574705301086 29.633647579027215 20.101639101546777 20-21 21.800900977870032 29.293151112615906 28.513365419199417 20.392582490314645 22-23 20.53790648244794 29.482581502606138 28.994023444520845 20.98548857042508 24-25 20.99546635880836 29.11494904700489 28.404847333665213 21.484737260521538 26-27 21.072828394099787 29.008947043176875 28.546817485692795 21.37140707703055 28-29 20.77259443505754 28.87916932993402 29.187117279416714 21.16111895559172 30-31 21.5785436570102 29.426496968319547 28.28578154043458 20.709177834235668 32-33 20.383909583608837 29.275175352220735 29.144934071736134 21.1959809924343 34-35 20.50832304144535 28.580049970044747 29.06023348361163 21.85139350489827 36-37 21.239672156189275 29.318829000812265 28.47093698290543 20.97056186009303 38-39 21.00115659425312 28.832886926718608 28.826720079564744 21.339236399463527 40-41 21.216057773296743 29.019278118878717 28.374383523785596 21.390280584038944 42-43 20.829984673655126 29.16277142823933 28.943627943430343 21.0636159546752 44-45 21.48236204477602 28.47608016995472 28.708471305180428 21.333086480088834 46-47 21.1818193633596 29.12741534494849 28.936896479661105 20.753868812030806 48-49 20.998188394443602 28.759971057978856 29.132016332551018 21.10982421502652 50-51 21.256231090949758 28.872612910708412 28.728581500159134 21.142574498182697 52-53 20.559388693165737 29.000356205291354 28.847627087216622 21.592628014326284 54-55 21.134769930840623 28.982135835271894 28.421208657805018 21.46188557608246 56-57 21.338703642261287 28.869928090631404 28.385063622794153 21.40630464431316 58-59 20.917642298273005 28.34373637965935 29.036608455910105 21.702012866157535 60-61 21.53889587255952 28.871770288609554 28.363093086287588 21.226240752543333 62-63 20.6972616820801 29.071081033271422 28.973818429650688 21.257838854997786 64-65 20.70108469953571 28.47925482677884 28.984931884249953 21.834728589435496 66-67 21.124785314388955 28.769803652284192 28.581724718080753 21.523686315246103 68-69 21.56843988722224 28.579011677984095 28.544489125498163 21.308059309295498 70-71 21.569754102409263 28.7796617055889 28.237194858664257 21.41338933333758 72-73 21.220447286863564 28.924303511187293 28.75864316967395 21.096606032275194 74-75 21.691279420651526 28.213336121825787 28.380019041643706 21.715365415878978 76-77 21.41322411456185 28.933702634215337 28.600762174160728 21.052311077062093 78-79 21.42042140075914 28.630147164603347 28.781892382820836 21.167539051816675 80-81 21.54465958453263 28.59026027180175 28.477641710531177 21.38743843313444 82-83 20.86529704580349 28.930484820424628 28.864616840520824 21.339601293251057 84-85 21.199429756482903 28.489198649828772 28.322323995887295 21.98904759780103 86-87 21.49375311719725 28.757281265439637 28.34544048192357 21.403525135439537 88-89 21.20053615653831 28.249246298767638 28.845890587769624 21.70432695692443 90-91 21.735744120434443 28.413521296614768 28.330669641587715 21.520064941363078 92-93 20.976883932701906 28.796630225706703 28.78983224172774 21.43665359986365 94-95 20.96890735209903 28.323465656620467 28.647582435470394 22.060044555810116 96-97 21.37561796123393 28.597379018760805 28.529710446040706 21.49729257396456 98-99 21.395477195822405 28.267896671770014 28.539889026495167 21.796737105912413 100 21.47777580187752 28.223070299411283 28.320487381796966 21.978666516914224 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 362.0 1 406.5 2 371.0 3 291.5 4 346.0 5 494.0 6 695.0 7 998.5 8 1513.5 9 2173.0 10 2945.0 11 3898.0 12 4842.0 13 5770.5 14 6730.0 15 7664.0 16 8440.0 17 9198.0 18 10122.0 19 10917.5 20 11782.5 21 12930.5 22 14438.0 23 16379.0 24 18869.5 25 22258.0 26 26758.5 27 32334.0 28 38827.0 29 46193.5 30 54267.0 31 62407.5 32 69954.5 33 77019.5 34 83622.0 35 89382.0 36 94107.5 37 97742.5 38 100220.0 39 101727.0 40 101975.5 41 100585.5 42 97377.0 43 92919.5 44 88273.5 45 83571.5 46 79285.5 47 75900.5 48 72778.0 49 71655.0 50 72682.5 51 68174.0 52 58241.0 53 50075.0 54 44595.0 55 40070.5 56 36041.0 57 32140.0 58 28307.0 59 24777.5 60 21165.0 61 17749.5 62 14596.0 63 11824.5 64 9426.0 65 7496.5 66 5926.0 67 4660.5 68 3617.0 69 2704.5 70 2065.0 71 1551.5 72 1176.5 73 902.0 74 689.0 75 511.5 76 367.5 77 271.5 78 179.5 79 120.0 80 91.0 81 59.0 82 38.5 83 28.0 84 14.5 85 14.5 86 13.0 87 11.0 88 8.0 89 2.5 90 1.5 91 1.0 92 0.5 93 1.0 94 2.5 95 2.0 96 1.0 97 1.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.988633253556475 2 24.642663731810003 3 6.042660237933022 4 24.26190878263621 5 12.279153006578195 6 9.817988419738851 7 7.807106933987076 8 8.844477830380036 9 5.09283037033155 10-11 6.90795769303185 12-13 10.056794911362282 14-15 7.442171483364294 16-17 8.072796867933391 18-19 6.655719185460813 20-21 7.98671162121715 22-23 5.827903266192664 24-25 5.2906808595713795 26-27 9.6954115690031 28-29 6.316250215941007 30-31 4.346188859579144 32-33 8.790963281293978 34-35 7.2091492991865085 36-37 6.73108987610324 38-39 6.203165064335862 40-41 8.765108591637599 42-43 6.483509871172992 44-45 6.5858416458489835 46-47 5.91204747014191 48-49 3.987464745810745 50-51 6.4459701040593025 52-53 4.529578580204858 54-55 4.928462868822389 56-57 4.758233418156902 58-59 3.5826796871815474 60-61 4.156956600224772 62-63 3.1101134151288754 64-65 4.463505484416338 66-67 2.5344971825764238 68-69 5.0353755044284885 70-71 2.5411743696948874 72-73 1.50687032087378 74-75 0.8767301969964305 76-77 1.187335860571016 78-79 1.77438483502107 80-81 2.2571998128834774 82-83 0.8084054915981964 84-85 1.2465182545367022 86-87 0.5255179187363035 88-89 0.7460980187776477 90-91 0.33758615803582 92-93 0.3492906459208016 94-95 0.5514114292480207 96-97 0.5506544225688924 98-99 0.47594368646724394 100 0.4543204700429165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2575935.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.14586630691284 #Duplication Level Percentage of deduplicated Percentage of total 1 97.80469380927393 94.03517015174997 2 1.831813609421794 3.5224261278130253 3 0.2088001252768444 0.6022580678920242 4 0.06661583170201257 0.2561934739498203 5 0.030736144773666034 0.1477576633099406 6 0.01759972814466902 0.10152846655412152 7 0.008268400164189787 0.05564807477307733 8 0.007079741603486306 0.054455031175702655 9 0.004142317839977914 0.03584400635189309 >10 0.01767819044661952 0.30338153334726875 >50 8.609750054675646E-4 0.06230613630182673 >100 0.0012637143836663936 0.28043285736189494 >500 2.4404288925852948E-4 0.17187171239786914 >1k 2.0336907436095393E-4 0.37072669702153027 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3210 0.1246149456410973 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2736 0.10621386020998201 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.8820855339905706E-5 0.0 0.0 0.0 6 0.0 3.8820855339905706E-5 0.0 3.8820855339905706E-5 0.0 7 0.0 3.8820855339905706E-5 0.0 7.764171067981141E-5 0.0 8 0.0 3.8820855339905706E-5 0.0 7.764171067981141E-5 0.0 9 0.0 3.8820855339905706E-5 0.0 7.764171067981141E-5 0.0 10-11 0.0 3.8820855339905706E-5 0.0 9.705213834976427E-5 0.0 12-13 0.0 3.8820855339905706E-5 0.0 1.5528342135962282E-4 0.0 14-15 0.0 3.8820855339905706E-5 0.0 2.1351470436948135E-4 0.0 16-17 0.0 3.8820855339905706E-5 0.0 2.911564150492928E-4 0.0 18-19 0.0 3.8820855339905706E-5 0.0 3.4938769805915133E-4 0.0 20-21 1.9410427669952853E-5 3.8820855339905706E-5 0.0 3.88208553399057E-4 0.0 22-23 3.8820855339905706E-5 3.8820855339905706E-5 0.0 4.076189810690099E-4 0.0 24-25 3.8820855339905706E-5 3.8820855339905706E-5 0.0 4.852606917488213E-4 0.0 26-27 3.8820855339905706E-5 3.8820855339905706E-5 0.0 6.793649684483497E-4 0.0 28-29 3.8820855339905706E-5 3.8820855339905706E-5 0.0 0.001184036087867124 0.0 30-31 3.8820855339905706E-5 3.8820855339905706E-5 0.0 0.0033385935592318908 0.0 32-33 3.8820855339905706E-5 3.8820855339905706E-5 0.0 0.0077447606403111885 0.0 34-35 3.8820855339905706E-5 3.8820855339905706E-5 0.0 0.015140133582563223 0.0 36-37 3.8820855339905706E-5 3.8820855339905706E-5 0.0 0.02682521103987484 0.0 38-39 3.8820855339905706E-5 3.8820855339905706E-5 0.0 0.041674188207388774 0.0 40-41 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.06283155436763738 0.0 42-43 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.08327073470409774 0.0 44-45 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.10865957409639607 0.0 46-47 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.1405509067581286 0.0 48-49 9.705213834976427E-5 3.8820855339905706E-5 0.0 0.17362627550772824 0.0 50-51 1.1646256601971711E-4 3.8820855339905706E-5 0.0 0.20839035146461382 0.0 52-53 1.1646256601971711E-4 7.764171067981141E-5 0.0 0.24629891670403176 0.0 54-55 1.1646256601971711E-4 7.764171067981141E-5 0.0 0.2891183201439477 0.0 56-57 1.1646256601971711E-4 7.764171067981141E-5 0.0 0.3372755911931008 0.0 58-59 1.3587299368966998E-4 9.705213834976427E-5 0.0 0.3937405252849936 0.0 60-61 1.5528342135962282E-4 1.1646256601971711E-4 0.0 0.4550192454390347 0.0 62-63 1.5528342135962282E-4 1.1646256601971711E-4 0.0 0.518336060498421 0.0 64-65 1.5528342135962282E-4 1.1646256601971711E-4 0.0 0.5907369557073452 0.0 66-67 1.5528342135962282E-4 1.5528342135962282E-4 0.0 0.6740076904114428 0.0 68-69 1.5528342135962282E-4 1.5528342135962282E-4 0.0 0.7716421415913057 0.0 70-71 1.5528342135962282E-4 1.5528342135962282E-4 0.0 0.8889005351454908 0.0 72-73 1.5528342135962282E-4 1.5528342135962282E-4 0.0 1.0104292227870657 0.0 74-75 1.5528342135962282E-4 1.5528342135962282E-4 0.0 1.1343453930320446 0.0 76-77 1.5528342135962282E-4 1.5528342135962282E-4 0.0 1.273401696859587 0.0 78-79 1.5528342135962282E-4 1.5528342135962282E-4 1.9410427669952853E-5 1.425715322785707 0.0 80-81 1.5528342135962282E-4 1.5528342135962282E-4 3.8820855339905706E-5 1.6070281276507365 1.9410427669952853E-5 82-83 1.5528342135962282E-4 1.5528342135962282E-4 3.8820855339905706E-5 1.7993660554322992 3.8820855339905706E-5 84-85 1.5528342135962282E-4 1.5528342135962282E-4 3.8820855339905706E-5 1.9955084270371728 3.8820855339905706E-5 86-87 1.5528342135962282E-4 1.5528342135962282E-4 3.8820855339905706E-5 2.209100773117334 3.8820855339905706E-5 88 1.5528342135962282E-4 1.5528342135962282E-4 3.8820855339905706E-5 2.395790266446941 3.8820855339905706E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTGCG 375 7.6397555E-11 18.76686 9 TCTATAC 970 0.0 16.7708 3 GTCTTAC 715 0.0 16.486473 1 TATACCG 215 3.2382697E-4 16.281485 5 GTACTAT 750 0.0 15.717106 1 CTATACT 1045 0.0 15.568767 4 TACGACT 200 0.00362899 15.077364 8 ACCGATC 205 0.004276563 14.709622 8 CCGATCC 310 2.0513222E-5 14.594042 9 ATACGAC 240 7.6131435E-4 14.548909 7 GTATAAT 1365 0.0 14.544458 1 CTGTCGC 625 1.8189894E-12 14.477291 9 TCTGTCG 630 3.6379788E-12 14.359394 8 GTATAGG 650 6.148184E-10 14.317202 1 GTATAAC 755 2.7284841E-11 13.969543 1 GTGTATA 1520 0.0 13.877637 1 CACGTAC 285 0.0011492047 13.777826 3 GTATATA 2165 0.0 13.468529 1 GTACATG 14035 0.0 13.305594 1 TAATACA 2195 0.0 13.2905 4 >>END_MODULE