FastQCFastQC Report
Wed 25 May 2016
SRR1295102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575935
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT91260.35427912583197946No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA68570.26619460506573345No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31860.12368324511293957No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC26190.10167182013521303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT39200.017.54597912-13
TAGGACG1700.001912013916.5979424
TCTGTCG4359.640644E-1116.1899288
ACTCGCG2053.642825E-416.0319778
TGCACCG2355.5971228E-516.0093635
TACACCG2104.3219508E-415.6758345
GCACCGA2206.223139E-414.9412096
AGTACTT44950.014.83148712-13
ACCGAGC1900.00405758214.8265658
CACCGAG3203.2412318E-614.6721237
GTATAGA10650.014.4873641
GTATAGG7400.014.1242321
CTCGCGT2000.005704445814.0849639
GTACTTT49100.014.05573114-15
CTGTCGC4352.719571E-814.0309999
TATACAG16000.013.8143295
CATGGGG35500.013.7770724
CTAATAC12650.013.7548123
ACTACGT2752.134763E-413.6804623
CCCGTAA2550.001224545413.6626571
Produced by FastQC (version 0.11.5)