##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295102_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2575935 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.289786038855794 33.0 33.0 33.0 27.0 33.0 2 29.951617179781323 33.0 33.0 33.0 27.0 33.0 3 30.789924823413635 33.0 33.0 33.0 27.0 33.0 4 35.07528023804949 37.0 37.0 37.0 33.0 37.0 5 35.37318371775685 37.0 37.0 37.0 33.0 37.0 6 35.08391516090274 37.0 37.0 37.0 33.0 37.0 7 34.985531079006265 37.0 37.0 37.0 33.0 37.0 8 34.98121458810102 37.0 37.0 37.0 33.0 37.0 9 34.9659541098669 37.0 37.0 37.0 33.0 37.0 10-11 34.9415608313098 37.0 37.0 37.0 33.0 37.0 12-13 34.8431944129025 37.0 37.0 37.0 30.0 37.0 14-15 36.25405047099403 37.0 37.0 40.0 27.0 40.0 16-17 36.244291490274406 37.0 37.0 40.0 27.0 40.0 18-19 36.28015574927163 38.5 37.0 40.0 27.0 40.0 20-21 36.28875340410375 40.0 37.0 40.0 27.0 40.0 22-23 36.25258013109803 38.5 37.0 40.0 27.0 40.0 24-25 36.22863232185595 37.0 37.0 40.0 27.0 40.0 26-27 36.19530539396375 37.0 37.0 40.0 27.0 40.0 28-29 36.141941275692126 37.0 37.0 40.0 27.0 40.0 30-31 36.05012995281325 37.0 37.0 40.0 27.0 40.0 32-33 35.996848134754956 37.0 37.0 40.0 27.0 40.0 34-35 35.892477682860786 37.0 37.0 40.0 27.0 40.0 36-37 35.887189700050655 37.0 37.0 40.0 27.0 40.0 38-39 35.830446226321705 37.0 37.0 40.0 27.0 40.0 40-41 35.77040317399313 37.0 37.0 40.0 27.0 40.0 42-43 35.67108079202309 37.0 37.0 40.0 27.0 40.0 44-45 35.567296729148836 37.0 37.0 40.0 27.0 40.0 46-47 35.455844576823566 37.0 37.0 40.0 27.0 40.0 48-49 35.35156865371215 37.0 35.0 40.0 27.0 40.0 50-51 35.259895338974005 37.0 33.0 40.0 27.0 40.0 52-53 35.17384464281901 37.0 33.0 40.0 27.0 40.0 54-55 35.054809224611645 37.0 33.0 40.0 27.0 40.0 56-57 34.92444956879735 37.0 33.0 40.0 27.0 40.0 58-59 34.80284692742635 37.0 33.0 40.0 27.0 40.0 60-61 34.651749753002306 37.0 33.0 40.0 27.0 40.0 62-63 34.46792892677804 37.0 33.0 40.0 22.0 40.0 64-65 34.30358471778209 37.0 33.0 40.0 22.0 40.0 66-67 34.08701034769899 37.0 33.0 40.0 22.0 40.0 68-69 33.87663974440349 37.0 33.0 38.5 22.0 40.0 70-71 33.64305912222164 37.0 33.0 37.0 22.0 40.0 72-73 33.41882365044149 37.0 33.0 37.0 22.0 40.0 74-75 33.185217794703675 37.0 33.0 37.0 22.0 40.0 76-77 32.941258028638146 37.0 33.0 37.0 22.0 38.5 78-79 32.70758132483933 37.0 33.0 37.0 22.0 37.0 80-81 32.494980075196 37.0 33.0 37.0 22.0 37.0 82-83 32.290296533103515 37.0 33.0 37.0 22.0 37.0 84-85 32.110910407289 33.0 33.0 37.0 22.0 37.0 86-87 32.12101004101423 33.0 33.0 37.0 22.0 37.0 88-89 32.130691574127454 33.0 33.0 37.0 22.0 37.0 90-91 32.12602918940112 33.0 33.0 37.0 22.0 37.0 92-93 32.078173168189416 33.0 33.0 37.0 22.0 37.0 94-95 32.10571986482578 33.0 33.0 37.0 22.0 37.0 96-97 32.05545365081029 33.0 33.0 37.0 22.0 37.0 98-99 31.978695891006566 33.0 33.0 37.0 22.0 37.0 100 31.44120135018935 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.573046031823317 1101 2 4.0079704070791635 1101 3 2.2756001662878447 1101 4 1.1289216392898709 1101 5 0.5102662172447765 1101 6 0.5541896327177653 1101 7 0.5491007812901643 1101 8 0.6202435195702165 1101 9 0.6277546559254432 1101 10-11 0.6266043559040781 1101 12-13 0.6958477623797066 1101 14-15 0.9570445607672866 1101 16-17 1.0309217024599633 1101 18-19 1.0413301240163335 1101 20-21 0.9994549959948529 1101 22-23 0.8986285015355477 1101 24-25 0.9521530739198738 1101 26-27 0.9992824682814856 1101 28-29 0.9246668849539788 1101 30-31 0.795098352470248 1101 32-33 1.0055556933961398 1101 34-35 1.090678073988002 1101 36-37 1.0509914206101456 1101 38-39 1.013113337285013 1101 40-41 0.9632187576544524 1101 42-43 1.0623210737368183 1101 44-45 0.9844546156588052 1101 46-47 1.1110769187413325 1101 48-49 1.0716224641763432 1101 50-51 1.0107679923744612 1101 52-53 1.0577706345184197 1101 54-55 1.0163415880595963 1101 56-57 1.0201569557135954 1101 58-59 1.0059062417261444 1101 60-61 1.0284832823147454 1101 62-63 1.0764018139138969 1101 64-65 1.025074227290986 1101 66-67 0.9762199721477245 1101 68-69 0.9699463097821734 1101 70-71 0.9300569805725516 1101 72-73 0.8549971529544322 1101 74-75 0.8617818253918941 1101 76-77 0.8146928880844087 1101 78-79 0.8574009535786544 1101 80-81 0.860924097266583 1101 82-83 0.9424301916014315 1101 84-85 1.0136359706761446 1101 86-87 0.9799542085745898 1101 88-89 0.8129511293704894 1101 90-91 1.0525254170376463 1101 92-93 1.0674837526603902 1101 94-95 1.0279975598748727 1101 96-97 0.9589659461157645 1101 98-99 0.9106643570651904 1101 100 1.011654912771096 1102 1 0.4217807997039422 1102 2 3.9997439191139925 1102 3 2.2261930961014222 1102 4 0.8987814824590501 1102 5 0.4530381698888064 1102 6 0.427356137756675 1102 7 0.4436928624138474 1102 8 0.37578191298641883 1102 9 0.45571843055061834 1102 10-11 0.5596123869396905 1102 12-13 0.6284315926079032 1102 14-15 0.8441942695874687 1102 16-17 0.7884420539129025 1102 18-19 0.8528597568618608 1102 20-21 0.8214341711897433 1102 22-23 0.8012298052431461 1102 24-25 0.8348810334472105 1102 26-27 0.8464277683729193 1102 28-29 0.7400402700696915 1102 30-31 0.6722250740173266 1102 32-33 0.7901874684533468 1102 34-35 1.0257138511869215 1102 36-37 0.8485434710918156 1102 38-39 0.8173605439690661 1102 40-41 0.9102270489088298 1102 42-43 0.9721861135941054 1102 44-45 1.01017308085234 1102 46-47 0.9751454239286019 1102 48-49 0.9588850337618098 1102 50-51 0.8159286073210197 1102 52-53 0.8800813292026106 1102 54-55 0.8115682455321149 1102 56-57 0.8895532193255988 1102 58-59 0.8661860076599339 1102 60-61 0.8934220021383155 1102 62-63 0.8997209524475309 1102 64-65 0.8317768402795522 1102 66-67 0.7990833434530131 1102 68-69 0.7413935547495143 1102 70-71 0.8067765577497141 1102 72-73 0.8512862487114816 1102 74-75 0.8102452469728334 1102 76-77 0.8021843214286193 1102 78-79 0.6357613888704705 1102 80-81 0.6558369162892248 1102 82-83 0.6764859299251356 1102 84-85 0.719970818044569 1102 86-87 0.8010020542360294 1102 88-89 0.7367592543462251 1102 90-91 0.7020997556226369 1102 92-93 0.8346941785543542 1102 94-95 0.7135219199269507 1102 96-97 0.772941520144169 1102 98-99 0.8820103588632726 1102 100 0.9766433797365686 1103 1 0.5134918566987814 1103 2 3.954778110905771 1103 3 2.3191257264032785 1103 4 1.0437306055736997 1103 5 0.4255271297996259 1103 6 0.4567355580171366 1103 7 0.37925856006710745 1103 8 0.411902286205752 1103 9 0.4967355738355437 1103 10-11 0.45551402649664396 1103 12-13 0.4385825712969762 1103 14-15 0.7052445144892232 1103 16-17 0.7214694538595907 1103 18-19 0.794909708736526 1103 20-21 0.6795604539941635 1103 22-23 0.6423994075975301 1103 24-25 0.6504967695144614 1103 26-27 0.6678451424207452 1103 28-29 0.7036401685319476 1103 30-31 0.696805745893343 1103 32-33 0.8188331370724669 1103 34-35 0.8860745871915725 1103 36-37 0.7132740214267983 1103 38-39 0.624700567687583 1103 40-41 0.5640791323106882 1103 42-43 0.7198271222586143 1103 44-45 0.7296081561496521 1103 46-47 0.7784406261143815 1103 48-49 0.663368169848404 1103 50-51 0.5987529285306081 1103 52-53 0.5909073957300777 1103 54-55 0.6527629371553232 1103 56-57 0.5821180607325473 1103 58-59 0.6216770706580306 1103 60-61 0.6121761154322982 1103 62-63 0.7282195820577186 1103 64-65 0.6298525014701752 1103 66-67 0.6515577129890389 1103 68-69 0.5580670297387726 1103 70-71 0.5507562036222282 1103 72-73 0.5496114856874854 1103 74-75 0.5044058275051029 1103 76-77 0.45861604225664365 1103 78-79 0.4918492618369612 1103 80-81 0.5484269831963218 1103 82-83 0.5955673616800183 1103 84-85 0.5870329350520969 1103 86-87 0.5106078448976561 1103 88-89 0.5878054979393781 1103 90-91 0.6107960789403073 1103 92-93 0.6357726213939934 1103 94-95 0.6377402781098489 1103 96-97 0.5850134902443749 1103 98-99 0.5577567135238759 1103 100 0.599473206637807 1104 1 0.42453008539174064 1104 2 3.6098584551826995 1104 3 2.12417326698505 1104 4 0.9294598419578506 1104 5 0.33422866506541027 1104 6 0.25411218738295105 1104 7 0.24267738562214447 1104 8 0.21207770173573692 1104 9 0.37094090184276496 1104 10-11 0.3545671129840997 1104 12-13 0.3456504627230217 1104 14-15 0.47901939723170983 1104 16-17 0.4487797028175038 1104 18-19 0.446494559970283 1104 20-21 0.5959065055590571 1104 22-23 0.44651509859927785 1104 24-25 0.4681675882325891 1104 26-27 0.45051689602765776 1104 28-29 0.4824373531622399 1104 30-31 0.39751028205306227 1104 32-33 0.5168243484570993 1104 34-35 0.6046844901415227 1104 36-37 0.48061178188251574 1104 38-39 0.43224412921086497 1104 40-41 0.4422279401788174 1104 42-43 0.4378957807559587 1104 44-45 0.5278134009088262 1104 46-47 0.485029808228731 1104 48-49 0.3809788980111435 1104 50-51 0.3090044962036771 1104 52-53 0.4199220163337003 1104 54-55 0.4384807104153907 1104 56-57 0.4197302886815564 1104 58-59 0.3828154943232107 1104 60-61 0.4209510873691187 1104 62-63 0.47465821734472513 1104 64-65 0.5202007822328056 1104 66-67 0.4729155943658583 1104 68-69 0.4701799180562318 1104 70-71 0.40127984045195575 1104 72-73 0.41212284144801714 1104 74-75 0.37628087086346085 1104 76-77 0.36632537310751445 1104 78-79 0.3589103546724104 1104 80-81 0.3727519637552561 1104 82-83 0.5516174135779011 1104 84-85 0.524205203673727 1104 86-87 0.408746028729162 1104 88-89 0.3907623232552453 1104 90-91 0.43489668618856214 1104 92-93 0.507034602340763 1104 94-95 0.4243552803330992 1104 96-97 0.47528172734802965 1104 98-99 0.4069825066762611 1104 100 0.3071476856272568 1105 1 0.4545066998740559 1105 2 3.7328532862408927 1105 3 2.15232398160882 1105 4 0.9070285700587846 1105 5 0.3474949086539496 1105 6 0.33103788003308665 1105 7 0.2827954798350234 1105 8 0.2752976117730839 1105 9 0.2894472778431805 1105 10-11 0.3081551129257747 1105 12-13 0.37809916107503483 1105 14-15 0.46169697934946896 1105 16-17 0.43808069590657794 1105 18-19 0.3857676800146095 1105 20-21 0.40883787489777745 1105 22-23 0.45453464751332007 1105 24-25 0.40413637705101735 1105 26-27 0.37546519335251105 1105 28-29 0.357109538316152 1105 30-31 0.3906859597889607 1105 32-33 0.505886183530869 1105 34-35 0.534065463291924 1105 36-37 0.4326870808631753 1105 38-39 0.4151673321783349 1105 40-41 0.33506221454502594 1105 42-43 0.39060032780738396 1105 44-45 0.40908119027478307 1105 46-47 0.4384460956319671 1105 48-49 0.4691942674930445 1105 50-51 0.3207388823272055 1105 52-53 0.2556037890195739 1105 54-55 0.3283225930291067 1105 56-57 0.2650356526352553 1105 58-59 0.31811609235081306 1105 60-61 0.3097008835412609 1105 62-63 0.409837115229287 1105 64-65 0.3019730703943537 1105 66-67 0.4879056581722594 1105 68-69 0.45576700193254993 1105 70-71 0.4158944301514538 1105 72-73 0.4538074272897106 1105 74-75 0.37629220568070565 1105 76-77 0.29937491620169254 1105 78-79 0.3745309922591957 1105 80-81 0.3469450374527838 1105 82-83 0.33011427098325896 1105 84-85 0.33339856902736287 1105 86-87 0.38685899377153277 1105 88-89 0.26480516091017137 1105 90-91 0.3419700098618925 1105 92-93 0.25898932803155716 1105 94-95 0.3157936487557542 1105 96-97 0.29953661225654926 1105 98-99 0.30786937326877606 1105 100 0.3145680137934157 1106 1 0.4209533637043421 1106 2 3.7879536354090533 1106 3 2.061580736524629 1106 4 0.8408373900129291 1106 5 0.2475692326384049 1106 6 0.060287027023242956 1106 7 0.11075523537539311 1106 8 0.052166243017140346 1106 9 0.0712258243311652 1106 10-11 0.09903585869110998 1106 12-13 0.05469381268233775 1106 14-15 0.18402799642224466 1106 16-17 0.10171135312236856 1106 18-19 0.1956267814410424 1106 20-21 0.10649939398885522 1106 22-23 0.14179251165631257 1106 24-25 0.09715749376037053 1106 26-27 0.03547455393046306 1106 28-29 0.06368679177116121 1106 30-31 0.050742738911807805 1106 32-33 0.27294592199009315 1106 34-35 0.25712432883074854 1106 36-37 0.1020568884015205 1106 38-39 0.05433860010306546 1106 40-41 0.0662017502938923 1106 42-43 -0.003127377617083482 1106 44-45 0.0782258252201018 1106 46-47 -0.004426870872201505 1106 48-49 -0.011054533158343816 1106 50-51 -0.1272822055642493 1106 52-53 -0.1452130543619461 1106 54-55 -0.13996520714862015 1106 56-57 -0.05067885314299758 1106 58-59 -0.08496144980715314 1106 60-61 -0.09956012856434882 1106 62-63 0.0363983249732982 1106 64-65 -0.07137415753322784 1106 66-67 -0.10143873983751917 1106 68-69 -0.1204054543376003 1106 70-71 -0.1829886273334438 1106 72-73 -0.16300783058334645 1106 74-75 -0.1376707874197649 1106 76-77 -0.11787572774738209 1106 78-79 -0.1187073459225303 1106 80-81 -0.08736351108912999 1106 82-83 -0.02434996233485265 1106 84-85 0.06553821690406281 1106 86-87 0.042070566935464626 1106 88-89 0.07551563480220125 1106 90-91 -0.13280450316798564 1106 92-93 -0.04557900340154575 1106 94-95 0.05644125770838215 1106 96-97 0.06598030260349574 1106 98-99 0.12076607786329774 1106 100 0.06899667010380028 1107 1 0.388167514812654 1107 2 3.504360305817656 1107 3 2.0450874570071065 1107 4 0.9339435642636928 1107 5 0.3210337194154249 1107 6 0.233129986984153 1107 7 0.10175339038220699 1107 8 0.06178779168462256 1107 9 0.10959664110923484 1107 10-11 0.1841386542717629 1107 12-13 0.15914802457432842 1107 14-15 0.09634116058556685 1107 16-17 0.02340405539501944 1107 18-19 0.05846981511744076 1107 20-21 0.11524739774728943 1107 22-23 0.11199222203344306 1107 24-25 0.1144137285104776 1107 26-27 0.0562274529234017 1107 28-29 0.05449508005028747 1107 30-31 0.014597356317928245 1107 32-33 0.17850001822775852 1107 34-35 0.2579172145233102 1107 36-37 0.0763102920822405 1107 38-39 -0.055699016512186006 1107 40-41 0.01764202245671953 1107 42-43 0.11128503891328734 1107 44-45 0.08963423386608582 1107 46-47 0.033764988411270735 1107 48-49 0.07584619617736621 1107 50-51 -0.12479666103852338 1107 52-53 0.027082156790783074 1107 54-55 0.0913615260603109 1107 56-57 0.04448526647826867 1107 58-59 0.12880844415839476 1107 60-61 0.03563513387329209 1107 62-63 0.1823561793315207 1107 64-65 0.08176407834472599 1107 66-67 0.09680970134331801 1107 68-69 0.048007386188437806 1107 70-71 0.09753269184195545 1107 72-73 0.08952987422722458 1107 74-75 0.07469425072273594 1107 76-77 0.09403503874092678 1107 78-79 0.05396095112875088 1107 80-81 0.10163594078360916 1107 82-83 0.18568430088417642 1107 84-85 0.1150675665913532 1107 86-87 0.10042444955619345 1107 88-89 0.09506098206529856 1107 90-91 0.02719952354018318 1107 92-93 0.1583921121085119 1107 94-95 0.045757399845516744 1107 96-97 -0.03504046265271299 1107 98-99 -0.030728697683592543 1107 100 -0.028572450007150252 1108 1 0.4871260765612888 1108 2 3.6678706601311966 1108 3 2.1573039054166436 1108 4 0.9787765044356149 1108 5 0.287789959984039 1108 6 0.15172946004260268 1108 7 0.11007517171801595 1108 8 0.13493122695255977 1108 9 0.08358009825474966 1108 10-11 0.12876719339795528 1108 12-13 0.15991546732863782 1108 14-15 0.04209171104365339 1108 16-17 0.07541836444787009 1108 18-19 0.09475171914612446 1108 20-21 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0.42716133296115544 2210 56-57 0.28221229391925107 2210 58-59 0.2730757617610351 2210 60-61 0.35804249885689643 2210 62-63 0.35970977093662526 2210 64-65 -0.07837824001191507 2210 66-67 0.24907575653353575 2210 68-69 0.35365180559197995 2210 70-71 0.3078564313667229 2210 72-73 0.32097637032619986 2210 74-75 -0.4152618765647773 2210 76-77 0.06558560371983901 2210 78-79 0.30074916646994865 2210 80-81 0.23278149611790155 2210 82-83 0.2185358129128474 2210 84-85 0.36390640067198277 2210 86-87 0.3992603709938791 2210 88-89 0.47847104874141166 2210 90-91 0.369592027084817 2210 92-93 0.3827687738282499 2210 94-95 0.299551228003633 2210 96-97 0.26286577521610965 2210 98-99 0.3511571772467228 2210 100 0.20071181470615684 2211 1 0.4665748108852483 2211 2 3.958041050509653 2211 3 2.285069640370679 2211 4 1.0733203724198788 2211 5 0.4941930562038337 2211 6 0.2704992799564536 2211 7 0.2437260621051962 2211 8 0.3252611754969408 2211 9 0.28399886896070115 2211 10-11 0.2650876961297257 2211 12-13 0.13554263141158884 2211 14-15 0.16045244061625397 2211 16-17 0.13747891290024228 2211 18-19 0.21203591401632593 2211 20-21 0.1975442100897027 2211 22-23 0.14365973827003842 2211 24-25 0.24625160808238888 2211 26-27 0.12198189982485985 2211 28-29 0.09247530264413228 2211 30-31 0.0968126144222694 2211 32-33 0.12033833801356053 2211 34-35 0.24205726451751275 2211 36-37 0.06320590204222043 2211 38-39 0.01644331431576518 2211 40-41 0.06014889681335234 2211 42-43 0.2678322584535593 2211 44-45 0.1595642241953641 2211 46-47 0.13449112877640346 2211 48-49 0.11570143908286212 2211 50-51 0.0031977932732942804 2211 52-53 0.0621573592514153 2211 54-55 0.15950635676618674 2211 56-57 0.032575467031776384 2211 58-59 0.10411241688459683 2211 60-61 0.10592727983658534 2211 62-63 0.2557733405023157 2211 64-65 0.025694644699754576 2211 66-67 0.04443493198714776 2211 68-69 0.10280156997286838 2211 70-71 0.04291490126037445 2211 72-73 0.17846286903029096 2211 74-75 0.1349387243743081 2211 76-77 0.08478506532436114 2211 78-79 0.07831280609331515 2211 80-81 0.11202440129522273 2211 82-83 -0.33458766068486767 2211 84-85 -0.015044473157889371 2211 86-87 0.07056153059576786 2211 88-89 0.08220612114464387 2211 90-91 0.13763319127759743 2211 92-93 0.1969989822531879 2211 94-95 0.12047366744925014 2211 96-97 0.05827321972641997 2211 98-99 0.15765264255011502 2211 100 -0.17453232968500387 2212 1 0.4633691602344143 2212 2 4.0855979181166475 2212 3 2.2782273982990198 2212 4 1.127044855182362 2212 5 0.6325577354295859 2212 6 0.5853402628123234 2212 7 0.4107527605433603 2212 8 0.20125402530939596 2212 9 0.23191424728211274 2212 10-11 0.2887258832887909 2212 12-13 0.3013639530806316 2212 14-15 0.3788904872223213 2212 16-17 0.4832804181523471 2212 18-19 0.37217201814258516 2212 20-21 0.3966580495524923 2212 22-23 0.4855940930739777 2212 24-25 0.25347232359952443 2212 26-27 0.1381708699815789 2212 28-29 0.2775108672599913 2212 30-31 0.2802922199502902 2212 32-33 0.451569815847769 2212 34-35 0.3884683051971791 2212 36-37 0.37988644187726095 2212 38-39 0.3620296921065602 2212 40-41 0.3866189935246922 2212 42-43 0.24967913571041578 2212 44-45 0.4423332689768529 2212 46-47 0.28147702386781503 2212 48-49 0.17554813847663553 2212 50-51 0.32590681726446036 2212 52-53 0.25149707340394656 2212 54-55 0.10892145469032499 2212 56-57 0.27222620154002897 2212 58-59 0.15016158162536897 2212 60-61 0.15320731200740312 2212 62-63 0.3083053632879995 2212 64-65 0.3397842817516121 2212 66-67 0.41229065987582914 2212 68-69 0.30777243174696167 2212 70-71 0.45757342585177696 2212 72-73 0.7101482177985474 2212 74-75 0.48745261792759464 2212 76-77 0.5264927247078646 2212 78-79 0.4410026172132362 2212 80-81 0.4029746908485521 2212 82-83 0.22789177818224005 2212 84-85 0.36738951231722794 2212 86-87 0.410665930283848 2212 88-89 0.3725172009404716 2212 90-91 0.24973848896850015 2212 92-93 0.3409978976998609 2212 94-95 0.5447174807058097 2212 96-97 0.13030125442389462 2212 98-99 0.5508864380912435 2212 100 0.5401222398531758 2213 1 -0.6642182249904138 2213 2 -25.815370064012914 2213 3 -14.731128637298424 2213 4 -6.134095165784611 2213 5 -2.055227774299105 2213 6 -1.1908907732245666 2213 7 -1.3974342715757615 2213 8 -1.1750478849910735 2213 9 -1.2311537927140321 2213 10-11 -1.231200823722773 2213 12-13 -1.1258412173656396 2213 14-15 -1.3341643693170582 2213 16-17 -1.4762243971491813 2213 18-19 -1.2121593907576127 2213 20-21 -1.412518796500592 2213 22-23 -1.5226160364613932 2213 24-25 -1.4508338152507463 2213 26-27 -1.5070788317207402 2213 28-29 -1.4720092549137433 2213 30-31 -1.5857675506786535 2213 32-33 -1.275826180740701 2213 34-35 -1.225058308159106 2213 36-37 -1.396137350219277 2213 38-39 -1.419803609938036 2213 40-41 -1.4375209001779794 2213 42-43 -1.4437242843946692 2213 44-45 -1.266466670836273 2213 46-47 -1.2854293707766757 2213 48-49 -1.4026019869947106 2213 50-51 -1.2848646294072168 2213 52-53 -1.4494219717340968 2213 54-55 -1.3795997973935314 2213 56-57 -1.477548800797841 2213 58-59 -1.395060135992722 2213 60-61 -1.3578360302766228 2213 62-63 -1.3822173836240808 2213 64-65 -1.3893828683084735 2213 66-67 -1.3069296599442808 2213 68-69 -1.4566017548337555 2213 70-71 -1.367589328316484 2213 72-73 -1.478295234312455 2213 74-75 -1.4352884582784071 2213 76-77 -1.5208394006692707 2213 78-79 -1.408916559407821 2213 80-81 -1.4421824454093297 2213 82-83 -1.3303919326198255 2213 84-85 -1.4698369836887082 2213 86-87 -1.3453367612506888 2213 88-89 -1.2423864684198378 2213 90-91 -1.17537912504379 2213 92-93 -1.3752698551430207 2213 94-95 -1.384256030741298 2213 96-97 -1.3004551877161994 2213 98-99 -1.1782151462438613 2213 100 -1.2643013548079125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 10.0 4 28.0 5 39.0 6 30.0 7 35.0 8 56.0 9 93.0 10 373.0 11 1748.0 12 5062.0 13 9597.0 14 12932.0 15 15085.0 16 15627.0 17 16219.0 18 16739.0 19 17612.0 20 18884.0 21 21446.0 22 23839.0 23 25248.0 24 25847.0 25 27171.0 26 29686.0 27 33212.0 28 38457.0 29 46316.0 30 55216.0 31 68340.0 32 85641.0 33 113828.0 34 154986.0 35 218301.0 36 329821.0 37 556993.0 38 586025.0 39 5389.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.20474435293796 21.238034560527826 10.3738259309493 30.183395155584915 2 12.516027002959685 23.144860294621783 36.53836442664282 27.80074827577571 3 15.247744993565442 27.722166902503364 28.974566516624062 28.05552158730713 4 10.311064626575307 18.903573188102808 37.89473193809844 32.89063024722345 5 11.365313710531003 38.66124457654892 35.449480538569404 14.523961174350683 6 29.41107467209743 39.919960193733154 17.78755153004367 12.881413604125743 7 25.210282778346283 33.49638929935514 23.66184657386354 17.63148134843504 8 23.858722057657825 37.470400740093154 21.169320777330842 17.501556424918178 9 24.27903483197254 16.953123816677262 22.44009061509286 36.32775073625734 10-11 22.661559242725883 28.18869634855178 29.187719967345217 19.962024441377125 12-13 23.434200952407398 25.781819361060197 29.68990154158884 21.094078144943566 14-15 21.213096875774337 26.98950823797898 26.84126267777233 24.95613220847435 16-17 20.260635577373783 30.817320145568196 27.960421947384344 20.961622329673677 18-19 20.227915581298898 29.641723789242157 30.03608702203044 20.094273607428498 20-21 21.43463312036839 28.9399145305617 29.158975834526878 20.466476514543025 22-23 20.35419140081602 29.123743715544098 29.37299598784997 21.149068895789913 24-25 20.71027135244444 28.722514906445458 29.188889786269762 21.378323954840344 26-27 20.760859563276256 28.715473404703474 29.19214144193149 21.331525590088784 28-29 20.299091453747778 28.678595016837942 29.82878995493185 21.193523574482427 30-31 21.20369379830889 29.06956674191387 28.988119588108447 20.7386198716688 32-33 19.97048414847758 29.120844565635984 29.82403459135123 21.0846366945352 34-35 20.123652290581912 28.569032692872103 29.952749614496486 21.354565402049495 36-37 20.964144297090055 29.100562787612656 29.1062114336749 20.829081481622392 38-39 20.639323049615516 28.97685251148484 29.41510691683746 20.96871752206219 40-41 20.815780309321458 28.84981444362009 29.162032124800106 21.172373122258342 42-43 20.516507777083913 29.00818230718506 29.44236812675811 21.03294178897292 44-45 21.067695351506146 28.294482000344757 29.270327163398452 21.36749548475065 46-47 20.915735384677507 29.145767666013583 29.235459614938097 20.70303733437081 48-49 20.812365463109785 28.899849122586975 29.294603923977665 20.993181490325576 50-51 21.032341887191887 28.851770006861262 28.832665651650718 21.28322245429613 52-53 20.371039863000668 29.19173111162456 28.86563930110147 21.571589724273306 54-55 20.898544815602218 29.014396520759917 28.515090621389948 21.571968042247917 56-57 21.09253270516026 28.769300538805698 28.584560500809353 21.553606255224693 58-59 20.531856570883665 28.416210322945368 29.39555682217038 21.656376284000583 60-61 21.257210169763304 28.85601519326157 28.52708410863345 21.35969052834167 62-63 20.282786356131034 29.170074657073492 29.06228735450316 21.484851632292315 64-65 20.343157811356978 28.51690453225979 29.226734912270075 21.913202744113157 66-67 20.85530818177847 28.82042022802394 28.748178672141574 21.57609291805602 68-69 20.99426338714506 28.712168663329983 28.829828833576098 21.463739115948854 70-71 21.094920570262392 28.727957298858865 28.43561113008482 21.74151100079392 72-73 20.735144076706298 28.947890081748472 28.97883820287328 21.33812763867195 74-75 21.294965257957816 28.204115613688064 28.629760964498423 21.871158163855693 76-77 20.893366246962884 28.93904021759494 28.82931086198311 21.338282673459066 78-79 20.97454353155501 28.81874205504567 28.933786158520913 21.272928254878405 80-81 21.068332728937317 28.638606384440905 28.630278143823475 21.662782742798303 82-83 20.35376825112421 29.038328452778323 28.86390967883457 21.7439936172629 84-85 20.66204086656198 28.498017860897146 28.616959795995268 22.22298147654561 86-87 20.909558921732756 28.79277303772817 28.489902503719396 21.807765536819677 88-89 20.567802535740256 28.341072865746774 29.043959068802444 22.047165529710526 90-91 21.004308093209175 28.49813460903467 28.617615137590903 21.87994216016525 92-93 20.198903032345328 28.976779505463874 28.90957781342543 21.91473964876537 94-95 20.208696516373834 28.3549378108994 29.06391510363297 22.37245056909379 96-97 20.697454897951538 28.67962895421169 28.703426383351736 21.919489764485036 98-99 20.533643074222322 28.420214708046633 28.84592726511127 22.200214952619778 100 20.638081635429113 28.354990721038465 28.68786739343768 22.319060250094733 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9.0 1 9.0 2 11.5 3 11.5 4 14.0 5 25.0 6 58.0 7 131.0 8 203.0 9 228.5 10 215.0 11 216.5 12 248.0 13 307.5 14 397.0 15 479.0 16 581.5 17 732.0 18 998.5 19 1355.0 20 1829.0 21 2633.5 22 3802.5 23 5483.0 24 7892.5 25 11198.5 26 15848.0 27 21962.0 28 29224.5 29 37778.0 30 47232.5 31 56763.5 32 66229.5 33 75058.0 34 83411.0 35 90768.5 36 96779.5 37 102166.5 38 106619.5 39 109418.5 40 110296.0 41 109801.0 42 107221.0 43 102677.0 44 97408.5 45 92310.0 46 87917.5 47 84443.5 48 80564.0 49 79098.0 50 90606.5 51 86737.0 52 64587.5 53 54110.0 54 49212.0 55 44691.5 56 40733.5 57 36814.0 58 32542.0 59 28561.5 60 24454.0 61 20284.0 62 16535.0 63 13198.5 64 10326.5 65 8119.0 66 6313.0 67 4771.0 68 3536.0 69 2664.0 70 1989.0 71 1472.5 72 1090.5 73 830.0 74 611.5 75 397.0 76 277.0 77 185.0 78 107.5 79 54.5 80 26.5 81 15.0 82 7.0 83 4.5 84 3.0 85 1.5 86 1.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.6803354898318474 2 5.44276156036546 3 0.0 4 0.006521903697104158 5 0.16432868065382084 6 0.05691137392830176 7 0.03687981257291042 8 0.04363464140205401 9 0.04223709060981741 10-11 0.043518178836034295 12-13 0.04237296360350708 14-15 0.044760446206911275 16-17 0.03579282862339306 18-19 0.0299308794670673 20-21 0.024398907581130735 22-23 0.024554191002490358 24-25 0.020924441028209177 26-27 0.013509657658287186 28-29 0.019488069380632665 30-31 0.02505886212190913 32-33 0.0012034465155370767 34-35 0.0032221309932121737 36-37 0.0037462125403009004 38-39 0.005745486590306045 40-41 0.006521903697104158 42-43 0.00755065636361166 44-45 0.008501767319439349 46-47 0.017585847468977286 48-49 0.025835279228707247 50-51 0.023525438335982855 52-53 0.02872743295153022 54-55 0.025621764524337766 56-57 0.01646004266412002 58-59 0.01517895443790313 60-61 0.012403263281099872 62-63 0.019119271254903557 64-65 0.02061387418548993 66-67 0.021254418298598372 68-69 0.02802865755541192 70-71 0.02851391824716074 72-73 0.025524712385988 74-75 0.025971152222396918 76-77 0.019701584085002145 78-79 0.015567162991302189 80-81 0.01401432877770596 82-83 0.009219953143227605 84-85 0.011976233872360909 86-87 0.009588751268956709 88-89 0.007686529357301328 90-91 0.007822402350991 92-93 0.00415383152136991 94-95 0.0016110654966060868 96-97 0.0010287526665075013 98-99 7.181858237882556E-4 100 0.11296868903912559 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2575935.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.66205467985647 #Duplication Level Percentage of deduplicated Percentage of total 1 92.2508090630055 80.86895468342186 2 6.282367037664935 11.01450405549423 3 0.906916858890463 2.3850658582241833 4 0.24334103851119554 0.8532710169528593 5 0.10910330033437537 0.4782109739832409 6 0.057755718178047664 0.3037790954999037 7 0.03377593817719189 0.20726076975467128 8 0.020301820447833848 0.14237594353589111 9 0.016673734220244422 0.13154884208392145 >10 0.07253110322536485 1.0357757759729092 >50 0.0028645769967726034 0.17244124622278473 >100 0.002093738480102175 0.3485625272088692 >500 8.885284048463606E-4 0.5507228401685349 >1k 4.886906226339319E-4 0.8850666335520704 >5k 8.885284047889671E-5 0.6224597379240843 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9126 0.35427912583197946 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6857 0.26619460506573345 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3186 0.12368324511293957 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2619 0.10167182013521303 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.8820855339905706E-5 0.0 5 3.8820855339905706E-5 0.0 0.0 1.5528342135962282E-4 0.0 6 3.8820855339905706E-5 0.0 0.0 2.3292513203943422E-4 0.0 7 3.8820855339905706E-5 0.0 0.0 3.4938769805915133E-4 0.0 8 3.8820855339905706E-5 0.0 0.0 3.88208553399057E-4 0.0 9 3.8820855339905706E-5 0.0 0.0 4.2702940873896275E-4 0.0 10-11 3.8820855339905706E-5 0.0 0.0 4.6585026407886844E-4 0.0 12-13 3.8820855339905706E-5 0.0 0.0 5.046711194187742E-4 0.0 14-15 3.8820855339905706E-5 0.0 0.0 5.434919747586798E-4 0.0 16-17 3.8820855339905706E-5 0.0 0.0 6.017232577685385E-4 0.0 18-19 3.8820855339905706E-5 0.0 0.0 6.987753961183027E-4 0.0 20-21 5.8231283009858555E-5 0.0 0.0 7.570066791281612E-4 0.0 22-23 7.764171067981141E-5 0.0 0.0 8.152379621380198E-4 0.0 24-25 7.764171067981141E-5 0.0 0.0 9.317005281577369E-4 0.0 26-27 7.764171067981141E-5 0.0 0.0 0.0011452152325272182 0.0 28-29 7.764171067981141E-5 0.0 0.0 0.0018439906286455208 0.0 30-31 7.764171067981141E-5 0.0 0.0 0.004658502640788684 0.0 32-33 7.764171067981141E-5 0.0 0.0 0.01094748120585341 0.0 34-35 7.764171067981141E-5 0.0 0.0 0.02123500787092842 0.0 36-37 7.764171067981141E-5 0.0 0.0 0.03678276043456065 0.0 38-39 7.764171067981141E-5 0.0 0.0 0.05863890199092757 0.0 40-41 7.764171067981141E-5 0.0 0.0 0.08789041648954651 0.0 42-43 7.764171067981141E-5 0.0 0.0 0.11553086549155939 0.0 44-45 7.764171067981141E-5 0.0 0.0 0.1503919935867947 0.0 46-47 7.764171067981141E-5 0.0 0.0 0.19346373258642008 0.0 48-49 7.764171067981141E-5 0.0 0.0 0.23777773895692245 0.0 50-51 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.28541092845898675 0.0 52-53 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.3352180858600857 0.0 54-55 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.3928476456121758 0.0 56-57 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.45721262376573946 0.0 58-59 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.5345243571751617 0.0 60-61 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.6146700130243969 0.0 62-63 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.6977466434517952 0.0 64-65 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.7936147457136924 0.0 66-67 7.764171067981141E-5 3.8820855339905706E-5 0.0 0.9001197623387236 0.0 68-69 7.764171067981141E-5 5.8231283009858555E-5 0.0 1.0235506718919538 0.0 70-71 7.764171067981141E-5 7.764171067981141E-5 0.0 1.1693812149763096 0.0 72-73 7.764171067981141E-5 7.764171067981141E-5 0.0 1.3181427326388282 0.0 74-75 7.764171067981141E-5 7.764171067981141E-5 0.0 1.4716403946528154 0.0 76-77 7.764171067981141E-5 7.764171067981141E-5 0.0 1.6440438132173365 0.0 78-79 7.764171067981141E-5 7.764171067981141E-5 0.0 1.8322667303328695 0.0 80-81 9.705213834976427E-5 7.764171067981141E-5 0.0 2.0491394386892523 0.0 82-83 1.1646256601971711E-4 7.764171067981141E-5 0.0 2.277930149634987 0.0 84-85 1.1646256601971711E-4 7.764171067981141E-5 0.0 2.5142715169443326 0.0 86-87 1.1646256601971711E-4 7.764171067981141E-5 0.0 2.7679269857352766 0.0 88 1.1646256601971711E-4 7.764171067981141E-5 0.0 2.984314433399911 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 3920 0.0 17.545979 12-13 TAGGACG 170 0.0019120139 16.597942 4 TCTGTCG 435 9.640644E-11 16.189928 8 ACTCGCG 205 3.642825E-4 16.031977 8 TGCACCG 235 5.5971228E-5 16.009363 5 TACACCG 210 4.3219508E-4 15.675834 5 GCACCGA 220 6.223139E-4 14.941209 6 AGTACTT 4495 0.0 14.831487 12-13 ACCGAGC 190 0.004057582 14.826565 8 CACCGAG 320 3.2412318E-6 14.672123 7 GTATAGA 1065 0.0 14.487364 1 GTATAGG 740 0.0 14.124232 1 CTCGCGT 200 0.0057044458 14.084963 9 GTACTTT 4910 0.0 14.055731 14-15 CTGTCGC 435 2.719571E-8 14.030999 9 TATACAG 1600 0.0 13.814329 5 CATGGGG 3550 0.0 13.777072 4 CTAATAC 1265 0.0 13.754812 3 ACTACGT 275 2.134763E-4 13.680462 3 CCCGTAA 255 0.0012245454 13.662657 1 >>END_MODULE