FastQCFastQC Report
Wed 25 May 2016
SRR1295101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711671
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1654.146124E-521.288085
GTACGAC1500.006496596618.7577763
GTACATG87000.018.1486131
ACCGATC1900.001210440217.7705253
TAGCGCT2602.8585213E-517.3148694
TACATGG86850.017.1016642
GTGTAGC7150.016.5187261
ACATGGG89550.016.338433
TATACTG13600.016.2343985
GTATAGG6900.016.2163241
TACGACC2100.002364657416.0780944
ATACCGT2701.05447354E-414.8642576
CATGGGG37600.014.8166484
GTGCTAG8700.014.2902461
TACACCG2509.913308E-414.0501345
GTAGCAC7255.456968E-1213.9713093
GTATAGA8651.8189894E-1213.6542071
GTGTATA14700.013.5319891
GTCTAGG7855.0931703E-1113.461961
TACACTG17900.013.4558335
Produced by FastQC (version 0.11.5)