FastQCFastQC Report
Wed 25 May 2016
SRR1295101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711671
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49950.1842037621820641No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36230.1336076537308545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95850.018.101731
GAGTACT36250.018.00107612-13
CAACGCA101100.016.976145
ATCAACG102700.016.8029213
TCAACGC103300.016.7056354
AACGCAG103300.016.6819026
TATCAAC103950.016.4336812
GTACATG117550.016.032531
TACATGG119150.015.8420142
CTATACG1800.002802801315.673864
ACATGGG113000.015.5626073
GTATAGG7550.015.1884621
ACGCAGA112850.015.0593667
CGCAGAG113150.015.0194398
AGTACTT43450.014.74804912-13
CATGGGG42000.014.4423414
CACGCGT2455.857146E-1014.36688984-85
TATACTG15200.014.2302145
GTACTTT46100.014.20564614-15
GTATAGA10900.013.7223231
Produced by FastQC (version 0.11.5)