FastQCFastQC Report
Wed 25 May 2016
SRR1295100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2785421
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGC2100.001155179817.894642
ATAGCGC1600.00900126217.7264333
CTAGTAC6600.017.1892683
GCACCGT2403.5675588E-516.8736526
GTATTAG11100.015.8010191
TCTATAC11850.015.7967193
TATACAG16350.015.1232985
TATACTG14250.014.8731375
AGTACCG2406.974356E-414.7182095
GTATATA25150.014.1966831
GTCTAGG8003.6379788E-1214.0939451
GTACATG113950.014.0726441
ACCGAGC2550.001072548913.9045028
TACATGG114900.013.5728272
GTACTGT12100.013.3873616
AACACCG4151.5331316E-613.3755845
GTATTGG10950.013.1572081
CATGGGG33750.012.94224554
GTATAAT14600.012.8711821
CTATACA14550.012.8660034
Produced by FastQC (version 0.11.5)