##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295100_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2785421
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.07694456241983	33.0	27.0	33.0	6.0	33.0
2	22.07616622406451	27.0	2.0	33.0	2.0	33.0
3	24.59543099588895	27.0	15.0	33.0	15.0	33.0
4	24.40529205459426	33.0	2.0	37.0	2.0	37.0
5	26.595717846602003	33.0	15.0	37.0	2.0	37.0
6	27.538031055269563	33.0	15.0	37.0	2.0	37.0
7	27.31764533978885	33.0	15.0	37.0	6.0	37.0
8	27.9844414901733	33.0	22.0	37.0	2.0	37.0
9	29.044827334898386	33.0	27.0	37.0	15.0	37.0
10-11	28.60450790024201	35.0	24.5	37.0	8.5	37.0
12-13	27.406124065266972	33.0	15.0	37.0	4.0	37.0
14-15	29.21082486274068	33.0	22.0	37.0	4.0	40.0
16-17	29.61013774937433	35.0	27.0	40.0	4.0	40.0
18-19	29.844087841658407	37.0	27.0	40.0	6.0	40.0
20-21	29.328179833497344	33.0	24.5	40.0	6.0	40.0
22-23	30.12801063106798	35.0	27.0	40.0	10.5	40.0
24-25	30.35423711532296	37.0	27.0	40.0	10.5	40.0
26-27	28.81603840137631	33.0	18.5	40.0	4.0	40.0
28-29	29.55461759640643	33.0	22.0	40.0	6.0	40.0
30-31	30.313331270210142	33.0	27.0	40.0	15.0	40.0
32-33	29.076302110165752	33.0	22.0	40.0	4.0	40.0
34-35	28.69287999910965	33.0	22.0	37.0	6.0	40.0
36-37	29.008347750663184	33.0	22.0	37.0	6.0	40.0
38-39	29.14697455070526	33.0	24.5	37.0	6.0	40.0
40-41	28.150296490189454	33.0	22.0	37.0	4.0	40.0
42-43	28.828859623015695	33.0	22.0	37.0	6.0	40.0
44-45	28.72477068996033	33.0	22.0	37.0	6.0	40.0
46-47	28.489125162767138	33.0	22.0	37.0	6.0	40.0
48-49	28.51331055520871	33.0	22.0	37.0	6.0	40.0
50-51	28.368183301554772	33.0	22.0	37.0	6.0	40.0
52-53	28.599006038943486	33.0	22.0	37.0	6.0	40.0
54-55	28.64156890466468	33.0	22.0	37.0	6.0	40.0
56-57	28.634069679233406	33.0	22.0	37.0	6.0	40.0
58-59	28.555297206418707	33.0	22.0	37.0	6.0	40.0
60-61	28.484914309183424	33.0	22.0	37.0	6.0	40.0
62-63	28.514911031402434	33.0	22.0	37.0	10.5	40.0
64-65	28.09663063500993	33.0	22.0	37.0	6.0	40.0
66-67	28.299198397656944	33.0	22.0	37.0	10.5	40.0
68-69	27.76605547240435	33.0	22.0	37.0	6.0	40.0
70-71	27.964554550281626	33.0	22.0	37.0	10.5	37.0
72-73	28.056999283052726	33.0	22.0	37.0	10.5	37.0
74-75	28.16711997934962	33.0	22.0	37.0	15.0	37.0
76-77	27.95666382209368	33.0	22.0	37.0	10.5	37.0
78-79	27.463535135263214	33.0	22.0	37.0	10.5	37.0
80-81	27.284459512583556	33.0	22.0	37.0	6.0	37.0
82-83	27.39370583477327	30.0	22.0	33.0	15.0	37.0
84-85	27.34442998024356	30.0	22.0	33.0	10.5	37.0
86-87	27.17902087332579	27.0	22.0	33.0	15.0	37.0
88-89	27.13620562205857	27.0	22.0	33.0	15.0	37.0
90-91	27.29656594101933	27.0	22.0	33.0	15.0	37.0
92-93	27.176461834674186	27.0	22.0	33.0	15.0	37.0
94-95	27.03586136530169	27.0	22.0	33.0	15.0	37.0
96-97	27.12875629931705	27.0	22.0	33.0	15.0	37.0
98-99	27.20625104786673	27.0	22.0	33.0	15.0	37.0
100	26.81983262135239	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.28489966774551
1101	2	11.284396209111609
1101	3	8.471286037825084
1101	4	12.832939106194619
1101	5	10.891478240450198
1101	6	10.067791759821798
1101	7	9.436291975455987
1101	8	8.973028916023932
1101	9	7.383221495354029
1101	10-11	7.842722345029038
1101	12-13	9.199596806193956
1101	14-15	8.155102483281922
1101	16-17	8.36506143338108
1101	18-19	8.164813556841725
1101	20-21	7.228289723324497
1101	22-23	7.406912573881279
1101	24-25	7.337958181975562
1101	26-27	8.66719228410749
1101	28-29	8.346223316836518
1101	30-31	5.634436991797767
1101	32-33	7.126654318534449
1101	34-35	8.74572701987966
1101	36-37	8.348778560141707
1101	38-39	8.298603158563445
1101	40-41	9.189382927575288
1101	42-43	8.758919315916923
1101	44-45	8.624352714629122
1101	46-47	7.41727720356808
1101	48-49	5.655346123873652
1101	50-51	6.93411650389239
1101	52-53	6.194354844875342
1101	54-55	4.45756056645126
1101	56-57	6.478475990088011
1101	58-59	6.345037607992232
1101	60-61	6.8834641187210295
1101	62-63	6.378180245114681
1101	64-65	6.831652212878108
1101	66-67	5.956239929061454
1101	68-69	6.978306807893453
1101	70-71	6.502130955050813
1101	72-73	5.737886185017107
1101	74-75	5.189356033030116
1101	76-77	5.1666960824808115
1101	78-79	5.297155609897267
1101	80-81	5.163500978348175
1101	82-83	4.770036390062671
1101	84-85	4.464779529070345
1101	86-87	4.333036090541352
1101	88-89	4.193120817662539
1101	90-91	3.9507419355511537
1101	92-93	3.84427627034054
1101	94-95	3.8515636920814345
1101	96-97	4.107517990788221
1101	98-99	3.837279397104993
1101	100	4.134411553744034
1102	1	6.738621055378932
1102	2	10.49855474815907
1102	3	8.180607744396042
1102	4	12.596823015304043
1102	5	10.746152167306562
1102	6	9.58785927358483
1102	7	6.996854875040398
1102	8	7.968736753275483
1102	9	6.498212593375314
1102	10-11	7.505321960095884
1102	12-13	9.088956615600875
1102	14-15	6.415089589976574
1102	16-17	7.565366796289439
1102	18-19	7.544970321051142
1102	20-21	7.058781719520265
1102	22-23	6.700484172372107
1102	24-25	7.009559324951216
1102	26-27	8.6045605639213
1102	28-29	7.825978863522142
1102	30-31	4.35594839219635
1102	32-33	4.655240084427859
1102	34-35	5.250259834240225
1102	36-37	5.533744794477286
1102	38-39	4.7597296298405105
1102	40-41	6.581635739681666
1102	42-43	7.187416400381029
1102	44-45	6.724184676048612
1102	46-47	5.8392811147542965
1102	48-49	4.087186457331494
1102	50-51	5.422418998332596
1102	52-53	6.185248195665828
1102	54-55	5.694051687408887
1102	56-57	6.606959459608692
1102	58-59	6.235199685427567
1102	60-61	6.156159355440344
1102	62-63	4.498624015671901
1102	64-65	6.24455807058369
1102	66-67	4.148481490409804
1102	68-69	6.45892345367648
1102	70-71	5.593845092842042
1102	72-73	5.3036419271151765
1102	74-75	4.5360611720866935
1102	76-77	3.678259180789258
1102	78-79	4.6227506035143975
1102	80-81	4.69489511428722
1102	82-83	4.06234787197814
1102	84-85	3.303305637725714
1102	86-87	3.517141715318523
1102	88-89	3.619373668183332
1102	90-91	3.5994570151866263
1102	92-93	3.330447599947753
1102	94-95	3.289782171550847
1102	96-97	3.5072078649533722
1102	98-99	3.31085039605453
1102	100	3.5050211988314253
1103	1	6.76774831953411
1103	2	10.914537061467271
1103	3	7.945738440910812
1103	4	12.101601193341107
1103	5	10.459928370533248
1103	6	9.372624258819986
1103	7	8.209265502907975
1103	8	8.224114287877232
1103	9	7.3238405064554755
1103	10-11	7.586000699516909
1103	12-13	8.957315600136763
1103	14-15	8.730149522697396
1103	16-17	8.235262548494916
1103	18-19	7.421220841027793
1103	20-21	8.603377866834602
1103	22-23	7.0727566985529435
1103	24-25	5.563054331267264
1103	26-27	6.343336275426953
1103	28-29	7.422292350211766
1103	30-31	6.412503576653389
1103	32-33	8.29247076441493
1103	34-35	7.492325056705091
1103	36-37	4.436399456888051
1103	38-39	6.69557403815039
1103	40-41	7.212427795113747
1103	42-43	7.823585006972788
1103	44-45	5.969235305701066
1103	46-47	6.768657321856043
1103	48-49	6.0159684012177514
1103	50-51	6.713876057288921
1103	52-53	6.545514259311268
1103	54-55	5.216781338011
1103	56-57	6.1732844804591345
1103	58-59	5.841515776036935
1103	60-61	5.473929625135391
1103	62-63	3.097557142492189
1103	64-65	5.324100508750874
1103	66-67	4.489509254887466
1103	68-69	5.557352016004543
1103	70-71	4.21409842164665
1103	72-73	4.800951097521615
1103	74-75	3.5753736521426376
1103	76-77	3.9397289922002976
1103	78-79	3.488398781372389
1103	80-81	4.432316846243186
1103	82-83	3.8380596565203646
1103	84-85	3.6988629455369164
1103	86-87	3.2580231083891604
1103	88-89	3.302745350864445
1103	90-91	2.994201676930995
1103	92-93	2.609165091683167
1103	94-95	3.093295099997661
1103	96-97	3.3229572786310833
1103	98-99	2.9817380449909585
1103	100	3.2239753272771807
1104	1	6.3703884198870995
1104	2	9.839833265307188
1104	3	7.5773600501426195
1104	4	12.316929195074781
1104	5	9.712740796299961
1104	6	8.336559696176469
1104	7	4.248937021913779
1104	8	6.706730466878955
1104	9	6.754296054000207
1104	10-11	7.2293066802714705
1104	12-13	7.647970019960642
1104	14-15	7.5321113652248854
1104	16-17	7.482238414602037
1104	18-19	7.064449306602302
1104	20-21	7.795327123564039
1104	22-23	6.668330623446572
1104	24-25	6.079977228668003
1104	26-27	8.437969182704624
1104	28-29	7.411178507084156
1104	30-31	6.59397869886482
1104	32-33	7.82928422371738
1104	34-35	8.283932371470904
1104	36-37	6.9394779764314585
1104	38-39	7.488584752748672
1104	40-41	7.4839270053757545
1104	42-43	7.573880273129074
1104	44-45	7.088934996890583
1104	46-47	6.985785676158759
1104	48-49	5.863214111836083
1104	50-51	5.961638327400152
1104	52-53	6.245142311471476
1104	54-55	5.047609250143797
1104	56-57	6.073404503963733
1104	58-59	3.979168983123323
1104	60-61	4.558262390301902
1104	62-63	4.032288735762535
1104	64-65	2.9823029483953043
1104	66-67	3.5754917181198493
1104	68-69	4.440012995446352
1104	70-71	2.9507935244376107
1104	72-73	2.73727841995559
1104	74-75	3.416192895911209
1104	76-77	4.0522706149347485
1104	78-79	4.420268564204861
1104	80-81	3.3425134264068
1104	82-83	3.210010830291896
1104	84-85	3.200278431069499
1104	86-87	1.091383808192873
1104	88-89	0.6721433162993442
1104	90-91	2.4110201788876253
1104	92-93	2.592956292459988
1104	94-95	2.5109066739308865
1104	96-97	3.002181960131214
1104	98-99	2.6844706634620756
1104	100	0.947794158464955
1105	1	5.863508964560097
1105	2	-2.8034162251245327
1105	3	0.507456094106562
1105	4	8.550837004379531
1105	5	9.024158504739383
1105	6	8.436653520499082
1105	7	5.663319521635877
1105	8	6.326756109169008
1105	9	5.350138053267806
1105	10-11	5.363945389168556
1105	12-13	5.505936227921335
1105	14-15	6.292314163310245
1105	16-17	6.473404415236999
1105	18-19	6.48107556640397
1105	20-21	7.411267861647918
1105	22-23	2.385023914267226
1105	24-25	4.508364572676708
1105	26-27	7.160232322557437
1105	28-29	6.222182853593214
1105	30-31	5.665059339425365
1105	32-33	7.5339871529633555
1105	34-35	6.686215139448414
1105	36-37	6.545729305499915
1105	38-39	4.446455536430115
1105	40-41	5.835948031193922
1105	42-43	6.095413719327013
1105	44-45	6.563591892757412
1105	46-47	5.8978733724086645
1105	48-49	5.294337620488449
1105	50-51	6.393686348307526
1105	52-53	6.029154911965769
1105	54-55	5.596074891731778
1105	56-57	5.476787510776564
1105	58-59	2.3465121608067427
1105	60-61	4.793667979905621
1105	62-63	-0.6639455871996951
1105	64-65	-0.13131134528294552
1105	66-67	1.2782094474422898
1105	68-69	3.329275758115351
1105	70-71	3.453774548116133
1105	72-73	3.089608780672979
1105	74-75	3.1074133846215446
1105	76-77	2.243315526314465
1105	78-79	3.6415198508864037
1105	80-81	1.9325270761860835
1105	82-83	1.3583635853873695
1105	84-85	1.6373889416264085
1105	86-87	0.08192878088435762
1105	88-89	1.8978298260500672
1105	90-91	1.6318854461812755
1105	92-93	2.0340904551519365
1105	94-95	1.350815699680279
1105	96-97	2.2811063104801654
1105	98-99	2.1385825639514593
1105	100	2.444042439257821
1106	1	6.152699494948461
1106	2	10.38145789146898
1106	3	7.028863704720713
1106	4	11.90955523363132
1106	5	10.091808628824005
1106	6	9.53778478077739
1106	7	6.779097612734418
1106	8	7.718032533612632
1106	9	7.111711025527029
1106	10-11	5.993848905698179
1106	12-13	8.178317674579812
1106	14-15	8.105915431708418
1106	16-17	7.935290461761163
1106	18-19	7.232888822620982
1106	20-21	8.413630261837152
1106	22-23	6.453460789279042
1106	24-25	5.173663691161426
1106	26-27	7.820815822682636
1106	28-29	6.363142972293613
1106	30-31	5.527204135243625
1106	32-33	7.610806944704507
1106	34-35	6.9978673021782
1106	36-37	6.228361750643717
1106	38-39	7.198729330136452
1106	40-41	6.89246258774476
1106	42-43	6.444889017296855
1106	44-45	4.914571294290003
1106	46-47	6.321916690449097
1106	48-49	6.068184256720972
1106	50-51	6.76397201098775
1106	52-53	5.999841775559297
1106	54-55	5.541029917314365
1106	56-57	5.1349506307057595
1106	58-59	5.114993402741746
1106	60-61	5.116293958538531
1106	62-63	5.126860341706951
1106	64-65	5.360017970551791
1106	66-67	3.411050232596139
1106	68-69	3.7582224816125915
1106	70-71	3.168125803822388
1106	72-73	3.02454166218779
1106	74-75	3.0213058474220738
1106	76-77	3.715635693363229
1106	78-79	4.339156899776036
1106	80-81	3.9198081879615287
1106	82-83	2.3157401054483913
1106	84-85	2.974380648012218
1106	86-87	3.45470385180953
1106	88-89	3.4703634365342495
1106	90-91	3.253859563669298
1106	92-93	3.0227001606407526
1106	94-95	1.6714092236082365
1106	96-97	1.6028690756635378
1106	98-99	2.045135039278634
1106	100	3.0557801754423046
1107	1	6.172718897340481
1107	2	10.433628057693866
1107	3	6.369443902613153
1107	4	11.521843968335862
1107	5	9.548600928891041
1107	6	8.227687422304125
1107	7	4.042342081759866
1107	8	6.588618309135775
1107	9	5.788947789999334
1107	10-11	4.768268087189519
1107	12-13	6.36283801680818
1107	14-15	6.4331516919483605
1107	16-17	7.203778360976138
1107	18-19	6.38065964068932
1107	20-21	5.732133378901903
1107	22-23	5.897853268149213
1107	24-25	4.759163704089705
1107	26-27	7.344316260573255
1107	28-29	6.650972682355903
1107	30-31	5.362777364469007
1107	32-33	5.297390242218775
1107	34-35	6.773077937916106
1107	36-37	6.663977669587542
1107	38-39	6.692107117610597
1107	40-41	7.853615926035594
1107	42-43	6.313987673816651
1107	44-45	4.298530543169068
1107	46-47	3.714230615077735
1107	48-49	4.435485662022408
1107	50-51	5.205269587875712
1107	52-53	4.302011809862925
1107	54-55	5.541862677571988
1107	56-57	5.53389422874017
1107	58-59	3.3002004537393965
1107	60-61	3.2569538691170266
1107	62-63	0.8009272729236194
1107	64-65	2.4617721480142585
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2104	6	9.501096510636753
2104	7	8.916775598150707
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2206	1	6.058130036945258
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2206	3	7.531054552901079
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2206	28-29	7.262983158312043
2206	30-31	6.652237782266706
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2206	76-77	2.743061875053005
2206	78-79	1.2575010875872792
2206	80-81	2.2468364366913853
2206	82-83	3.415970442472336
2206	84-85	3.746953001137829
2206	86-87	-1.0637379679015027
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2206	90-91	0.6211730632477348
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2206	94-95	2.8757643465806204
2206	96-97	1.9147479922189454
2206	98-99	1.6038780856463433
2206	100	1.9234269015446657
2207	1	6.50691553808149
2207	2	10.841377468608485
2207	3	7.92207111295593
2207	4	12.174995496537345
2207	5	10.528486513284903
2207	6	9.402250648898285
2207	7	6.07617041543573
2207	8	7.081926434173521
2207	9	6.317882984464276
2207	10-11	7.06558238217238
2207	12-13	8.877125085782001
2207	14-15	8.499438124004534
2207	16-17	7.577369385432405
2207	18-19	4.51125961458273
2207	20-21	7.796076317955599
2207	22-23	6.5703432677863916
2207	24-25	4.567233886854847
2207	26-27	4.223329376797
2207	28-29	5.401009742634262
2207	30-31	5.598813152527125
2207	32-33	7.021639967698725
2207	34-35	6.529957983918159
2207	36-37	6.966624246449317
2207	38-39	7.142516715882373
2207	40-41	4.473867141655987
2207	42-43	5.977389685661979
2207	44-45	4.228016398573072
2207	46-47	5.837841106995395
2207	48-49	2.627573035911471
2207	50-51	5.749750473164717
2207	52-53	3.1418476694574835
2207	54-55	3.4501318985464806
2207	56-57	5.548204168376039
2207	58-59	4.055235143025158
2207	60-61	3.7719496834686232
2207	62-63	4.464622926188465
2207	64-65	3.700299857652716
2207	66-67	3.8483554110807034
2207	68-69	3.851259703385601
2207	70-71	3.943883445342891
2207	72-73	4.133209500904325
2207	74-75	3.890774233266484
2207	76-77	4.326074775710595
2207	78-79	4.45602780304263
2207	80-81	4.554603460198585
2207	82-83	4.024468265801318
2207	84-85	3.4422402753138037
2207	86-87	3.6535662272963734
2207	88-89	3.4063573532019618
2207	90-91	1.484424191164866
2207	92-93	-0.48596178469913553
2207	94-95	1.6460934911697365
2207	96-97	-0.7609608206249163
2207	98-99	0.9774955038076776
2207	100	2.414843767149403
2208	1	1.1400573959302633
2208	2	8.638295436082267
2208	3	6.7537060361911045
2208	4	11.891044653163558
2208	5	10.131146576691215
2208	6	9.193962907122955
2208	7	7.791495988566737
2208	8	7.950344703063767
2208	9	6.7421179891308185
2208	10-11	6.859969368361966
2208	12-13	7.219291871457095
2208	14-15	7.990123146762748
2208	16-17	7.316340102892795
2208	18-19	6.510000611635018
2208	20-21	6.769477899882663
2208	22-23	6.017833332833646
2208	24-25	6.1945756901763325
2208	26-27	8.095218011669306
2208	28-29	7.146055171965063
2208	30-31	6.140430994241381
2208	32-33	8.418993269918445
2208	34-35	6.65302664747346
2208	36-37	7.01534825285022
2208	38-39	7.343145772764501
2208	40-41	7.818838423120717
2208	42-43	7.23436011050018
2208	44-45	6.142702882937055
2208	46-47	4.6823066549131696
2208	48-49	4.91969342655792
2208	50-51	6.153746826007321
2208	52-53	5.716531846961807
2208	54-55	6.492208199351499
2208	56-57	6.411509367554391
2208	58-59	5.800288516471532
2208	60-61	5.308317370697701
2208	62-63	3.449286169972659
2208	64-65	3.4800007246288907
2208	66-67	2.987677261236783
2208	68-69	5.716109837924076
2208	70-71	5.6244261544273435
2208	72-73	4.787359321516437
2208	74-75	2.1782335883141215
2208	76-77	3.5116483259422573
2208	78-79	4.183644790527524
2208	80-81	3.692105480721665
2208	82-83	1.4659249562532253
2208	84-85	2.0516010329605514
2208	86-87	1.2722603730211688
2208	88-89	0.9561411743053831
2208	90-91	2.1519146486793055
2208	92-93	0.9056058181494961
2208	94-95	-0.7887253471770137
2208	96-97	1.6289996040212316
2208	98-99	0.4888113250211461
2208	100	1.8860088714872774
2209	1	5.4152780880413225
2209	2	9.71621722891188
2209	3	5.8329633572431945
2209	4	-3.3247801671996378
2209	5	0.5544080327048277
2209	6	4.71007260197262
2209	7	2.494685924789639
2209	8	5.395247424908703
2209	9	5.320853180630376
2209	10-11	5.452282362868097
2209	12-13	7.57174458636263
2209	14-15	6.945523582076511
2209	16-17	6.601747291442592
2209	18-19	5.617122368878523
2209	20-21	6.182568798956208
2209	22-23	5.733250088579915
2209	24-25	5.53372195018213
2209	26-27	7.227835084462296
2209	28-29	6.506943405841444
2209	30-31	4.388553099356034
2209	32-33	6.48419588085406
2209	34-35	5.724176749423034
2209	36-37	6.361266190285011
2209	38-39	6.957803953645609
2209	40-41	7.570281458890101
2209	42-43	7.1105634989335975
2209	44-45	6.772018878830611
2209	46-47	7.016756560216411
2209	48-49	5.464315508308093
2209	50-51	5.0623676843572625
2209	52-53	5.200929579662947
2209	54-55	5.6975742739485185
2209	56-57	5.2409796658656695
2209	58-59	3.3820145901205194
2209	60-61	4.36036704360977
2209	62-63	4.505681111633958
2209	64-65	5.264636441443212
2209	66-67	4.823916788885278
2209	68-69	4.164157510287829
2209	70-71	4.010242741938594
2209	72-73	1.2671009316167172
2209	74-75	2.587811583294137
2209	76-77	3.5649878338472902
2209	78-79	3.784176473018416
2209	80-81	3.960905635161282
2209	82-83	3.379166924111182
2209	84-85	3.386647936381369
2209	86-87	1.9569293785032542
2209	88-89	2.322302342302347
2209	90-91	2.5860329747473187
2209	92-93	2.6715658902030235
2209	94-95	1.1251885949719949
2209	96-97	0.4666954100015097
2209	98-99	0.5403264665091498
2209	100	0.5559930791431356
2210	1	1.7087205872505535
2210	2	-17.15912903147593
2210	3	-7.67712312398446
2210	4	-8.853435910089917
2210	5	-2.1958190134664193
2210	6	2.3698514026895836
2210	7	-9.604968174262398
2210	8	-9.641854555365237
2210	9	-4.346649982440294
2210	10-11	-1.5055721167109262
2210	12-13	3.271959171952446
2210	14-15	-6.2065021010988275
2210	16-17	-2.195091914339116
2210	18-19	-0.5528714128120917
2210	20-21	2.100279239006433
2210	22-23	2.1316157754934295
2210	24-25	1.578115167791811
2210	26-27	3.802861263664628
2210	28-29	3.11228983396785
2210	30-31	2.3087972908080765
2210	32-33	4.360381433289142
2210	34-35	2.1360639643872332
2210	36-37	3.7153539798737114
2210	38-39	2.5698087054404084
2210	40-41	3.842199501812015
2210	42-43	1.724874656425765
2210	44-45	2.981800854599573
2210	46-47	2.6815285795015527
2210	48-49	2.6005719662440505
2210	50-51	1.8838114463984965
2210	52-53	2.001974231677252
2210	54-55	2.3035763802660334
2210	56-57	2.3037980696651914
2210	58-59	1.1853141281205026
2210	60-61	2.321138976629353
2210	62-63	1.218541090642674
2210	64-65	2.3423024068027942
2210	66-67	2.372368980989105
2210	68-69	2.863567250149231
2210	70-71	2.0940671100433406
2210	72-73	0.7372668250883301
2210	74-75	0.2466663240661191
2210	76-77	-0.24340886074385892
2210	78-79	0.023103628938628162
2210	80-81	0.4663733143300668
2210	82-83	0.48679699341118265
2210	84-85	-1.5660312084393446
2210	86-87	-1.424726848432691
2210	88-89	-1.605390640952546
2210	90-91	-2.1133438164612635
2210	92-93	-1.6078707357590787
2210	94-95	-0.7783503299673811
2210	96-97	-0.33328769632558775
2210	98-99	-0.6781482624732043
2210	100	-0.42652137033879
2211	1	1.0521042761792252
2211	2	-17.15912903147593
2211	3	-8.096672128005782
2211	4	-19.07791687894805
2211	5	-21.424408978864342
2211	6	-14.207714082964138
2211	7	-12.096867135754785
2211	8	-18.511544951396463
2211	9	-16.44946429576499
2211	10-11	-10.57405884367516
2211	12-13	-22.555679236352052
2211	14-15	-18.04166980092916
2211	16-17	-15.6223966597667
2211	18-19	-19.40030929496018
2211	20-21	-9.10447826812997
2211	22-23	-4.802415806137631
2211	24-25	-2.2099023603641292
2211	26-27	-17.669111973165876
2211	28-29	-3.9002666115038203
2211	30-31	-5.49608599398438
2211	32-33	-1.2535883756201116
2211	34-35	-7.077449020297898
2211	36-37	-8.052339281850692
2211	38-39	-3.457985373240348
2211	40-41	-6.7056018524339756
2211	42-43	-2.5015122934257157
2211	44-45	-0.4193429455277915
2211	46-47	0.6467100734027689
2211	48-49	0.5113057351093069
2211	50-51	-5.698131819779196
2211	52-53	-1.4177364268309844
2211	54-55	-0.6992790425072108
2211	56-57	-8.090946962687973
2211	58-59	-3.0237685271991808
2211	60-61	-1.5452265361894462
2211	62-63	-0.6615503298682981
2211	64-65	-4.362238670359389
2211	66-67	-3.2960174077476303
2211	68-69	0.04078768919924869
2211	70-71	-0.38885858010553065
2211	72-73	-1.3031184625720407
2211	74-75	-1.8638387961059593
2211	76-77	-1.7057789081268808
2211	78-79	-1.2636573194788951
2211	80-81	-1.1398792741457058
2211	82-83	-1.7377470451899484
2211	84-85	-1.757881406388158
2211	86-87	-2.0945392646551397
2211	88-89	-2.717027175534586
2211	90-91	-2.5735100968048776
2211	92-93	-2.3736728236848705
2211	94-95	-2.58477548349461
2211	96-97	-3.2824349466406595
2211	98-99	-3.118519355266443
2211	100	-2.6065824249673177
2212	1	-21.28457526050031
2212	2	-17.15912903147593
2212	3	-20.091266722600377
2212	4	-19.07791687894805
2212	5	-21.424408978864342
2212	6	-22.25147212672218
2212	7	-22.590046028933678
2212	8	-23.00755524740676
2212	9	-24.386658632959325
2212	10-11	-11.571671124079028
2212	12-13	-22.555679236352052
2212	14-15	-23.999970959230318
2212	16-17	-24.408110945480985
2212	18-19	-19.040438634898315
2212	20-21	-24.141286504938204
2212	22-23	-19.10884515271994
2212	24-25	-25.788666839128606
2212	26-27	-17.765974938288878
2212	28-29	-18.656754402217235
2212	30-31	-6.663993890955375
2212	32-33	-17.828059979154812
2212	34-35	-14.319607294387337
2212	36-37	-18.223943800873947
2212	38-39	-24.3111385263935
2212	40-41	-10.811175330856404
2212	42-43	-14.432495803567924
2212	44-45	-17.70547950546932
2212	46-47	-17.73861097558941
2212	48-49	-18.349710429922155
2212	50-51	-9.639668248313715
2212	52-53	-2.4904232081219746
2212	54-55	-24.25989680312497
2212	56-57	-24.201500373241384
2212	58-59	-13.267473417557989
2212	60-61	-6.85695227449262
2212	62-63	-3.9381401947946806
2212	64-65	-18.077779395460343
2212	66-67	-18.34183908301481
2212	68-69	-15.200497245011112
2212	70-71	-10.297169704669047
2212	72-73	-18.26402758256338
2212	74-75	-7.024026900079914
2212	76-77	-12.021958950501952
2212	78-79	-5.438579988665424
2212	80-81	-2.9513931370936035
2212	82-83	-3.5256940197526028
2212	84-85	-5.816791367974982
2212	86-87	-5.54520159046087
2212	88-89	-3.966200467920114
2212	90-91	-4.800718731279286
2212	92-93	-6.067720873373453
2212	94-95	-4.281492276769722
2212	96-97	-5.921438221972807
2212	98-99	-4.200243198749366
2212	100	-4.134056088196239
2213	1	-21.28457526050031
2213	2	-17.15912903147593
2213	3	-20.091266722600377
2213	4	-19.07791687894805
2213	5	-21.424408978864342
2213	6	-22.25147212672218
2213	7	-10.773138977651627
2213	8	-10.653388580740094
2213	9	-4.34258812013881
2213	10-11	-17.93992036109918
2213	12-13	-16.573307441480257
2213	14-15	-23.999970959230318
2213	16-17	-18.36263818907073
2213	18-19	-18.969617098642985
2213	20-21	-18.126462786989485
2213	22-23	-20.35236135608318
2213	24-25	-7.959940877590146
2213	26-27	-23.787644791698693
2213	28-29	-24.675556186793397
2213	30-31	-19.90623118381707
2213	32-33	-23.916651438683175
2213	34-35	-18.45629904133542
2213	36-37	-24.34217197168338
2213	38-39	-11.933534359726831
2213	40-41	-17.61990179798392
2213	42-43	-23.862387454300876
2213	44-45	-17.777368141052985
2213	46-47	-17.64870498451229
2213	48-49	-6.489206281027709
2213	50-51	-24.056175577822955
2213	52-53	-18.430797788329848
2213	54-55	-18.387701290304456
2213	56-57	-18.278022809138818
2213	58-59	-18.77949877667943
2213	60-61	-20.07374196782988
2213	62-63	-18.32856334219381
2213	64-65	-18.402309733867952
2213	66-67	-9.086897333002554
2213	68-69	-23.444283095871533
2213	70-71	-18.095646119705574
2213	72-73	-9.864629080332662
2213	74-75	-4.711709984602145
2213	76-77	-3.5259917892772634
2213	78-79	-5.039494798441375
2213	80-81	-3.663043441059873
2213	82-83	-3.7034028280332336
2213	84-85	-3.361778452472702
2213	86-87	-3.687961172139552
2213	88-89	-3.166378879573788
2213	90-91	-3.2781951218024012
2213	92-93	-4.0063151672647095
2213	94-95	-4.070807188276316
2213	96-97	-3.6832192130499237
2213	98-99	-4.21321960152919
2213	100	-3.487859649994544
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	781.0
3	861.0
4	6353.0
5	21064.0
6	22859.0
7	27276.0
8	24526.0
9	25244.0
10	28258.0
11	32711.0
12	39723.0
13	53908.0
14	40382.0
15	44234.0
16	51698.0
17	54894.0
18	55062.0
19	55165.0
20	53179.0
21	50911.0
22	52499.0
23	57583.0
24	63371.0
25	69690.0
26	77226.0
27	83895.0
28	91794.0
29	96940.0
30	100838.0
31	109269.0
32	123213.0
33	151503.0
34	188468.0
35	229530.0
36	264517.0
37	248727.0
38	87056.0
39	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.30583850479305	21.939655616011297	14.131127477174724	23.623378402020926
2	14.716852590537089	21.832997911227665	40.8616454677941	22.588504030441143
3	17.436740229394474	27.294833907824678	30.372499261589187	24.895926601191665
4	12.061922836819527	18.373805409976534	40.27164226599513	29.292629487208803
5	12.379403459605761	38.31589883586113	36.84716523867911	12.457532465853998
6	28.86967109714701	39.851416586724156	18.956285187227934	12.322627128900903
7	25.617116573085813	32.554729602255726	24.756220107213423	17.071933717445035
8	23.12560991493753	37.02219238746005	22.49424400605156	17.35795369155086
9	24.023183322919937	17.697285882006845	22.869962208774943	35.40956858629827
10-11	22.370777758142978	28.134491392392686	30.050845466371793	19.443885383092542
12-13	23.327810296466556	25.69309851701793	29.915868037970572	21.06322314854494
14-15	20.82803722949547	27.436020901956482	27.18749250087432	24.548449367673722
16-17	20.197724247519236	31.044446253836128	28.155726021383444	20.602103477261196
18-19	20.252021584540127	30.08549657394262	29.73163796553534	19.930843875981907
20-21	21.522402795871777	29.45625727596561	28.792101095897298	20.229238832265317
22-23	20.60353139161267	29.49025549877976	29.087332773980428	20.818880335627142
24-25	20.845363104466756	29.43180778744318	28.66167964662979	21.06114946146027
26-27	20.848439304165474	29.115984394050624	28.8493538350769	21.186222466707
28-29	20.72515723380192	29.128946938281675	29.243402792117884	20.902493035798525
30-31	21.27166895919823	29.53083048609607	28.63857644562731	20.558924109078387
32-33	20.407293451884602	29.277717069703353	29.192914127847015	21.12207535056503
34-35	20.40267066922002	28.854457310541363	29.20387692462119	21.538995095617423
36-37	21.088245892843897	29.447522885390327	28.634126777046866	20.830104444718913
38-39	20.909489153914798	29.12431416225437	28.87314023713017	21.093056446700665
40-41	20.922599400992485	29.184309158982668	28.688205504118052	21.204885935906795
42-43	20.789781218693054	29.17387969016245	29.00290072649088	21.033438364653616
44-45	21.19863635117166	28.830837803077642	28.950698715409196	21.019827130341497
46-47	20.920369118626336	29.271019078229987	29.108353100562113	20.700258702581564
48-49	20.869038841934056	28.956467121129787	29.195870568170502	20.978623468765655
50-51	20.982118854914784	29.082691335187317	28.967408285886375	20.96778152401152
52-53	20.488037666926324	29.056944374287163	29.00234829601367	21.45266966277284
54-55	20.95826957513236	29.227825665719404	28.695950881367473	21.117953877780764
56-57	21.113963910147486	29.02713930122996	28.607177943382172	21.251718845240386
58-59	20.830262876169705	28.56111574924503	29.169333671857682	21.43928770272758
60-61	21.163059621118503	29.08225348706263	28.699215103865527	21.055471787953337
62-63	20.66024379680166	29.165980919587298	29.045103468308813	21.12867181530223
64-65	20.61956651595137	28.7860306414387	29.13833716680026	21.456065675809675
66-67	20.881381501080096	28.910155190071446	28.784305906241542	21.424157402606912
68-69	21.38343481489583	28.826503591296156	28.717267814596838	21.072793779211185
70-71	21.255751832082208	28.936873915315154	28.584080661139122	21.22329359146352
72-73	21.059358776769074	29.01914833521328	28.878509603108633	21.042983284909013
74-75	21.34977000064983	28.605341009296176	28.61920885741638	21.425680132637616
76-77	21.230433535187597	29.021137898793242	28.695382110411078	21.053046455608083
78-79	21.31051183527755	28.81598395774148	28.852715648618148	21.02078855836282
80-81	21.288018693320403	28.750115341411497	28.73880681819228	21.22305914707582
82-83	20.824234672325566	29.027300979375966	28.90962324775641	21.238841100542057
84-85	21.005121187814733	28.762514088104002	28.554428182706694	21.677936541374578
86-87	21.257290605470264	28.897308954859312	28.554801468601042	21.290598971069382
88-89	21.144055350230943	28.519091026400456	28.88561672582079	21.451236897547815
90-91	21.365143044422933	28.554561524606004	28.650829557294976	21.42946587367609
92-93	20.984497896544003	28.803911267742127	28.878336668098985	21.333254167614882
94-95	20.883853607371396	28.5598965836764	28.81814799480748	21.738101814144716
96-97	21.161251978689478	28.64784246882569	28.72677300705817	21.464132545426658
98-99	21.295140359287874	28.456828203609337	28.697687451700492	21.550343985402296
100	21.265123633429514	28.398385364460427	28.599834713795317	21.736656288314745
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	341.0
1	386.0
2	360.0
3	329.0
4	437.0
5	661.0
6	1103.5
7	1732.0
8	2564.0
9	3615.5
10	4747.0
11	5895.5
12	6987.0
13	7931.5
14	8712.5
15	9630.5
16	10590.5
17	11316.5
18	12302.0
19	13317.5
20	14321.0
21	15649.0
22	17323.5
23	19599.5
24	22612.5
25	26374.0
26	31164.0
27	37480.5
28	45158.5
29	53367.5
30	62193.0
31	71299.0
32	79479.0
33	86983.5
34	94215.5
35	100228.0
36	104776.0
37	108364.0
38	110349.5
39	110205.5
40	108862.0
41	106394.0
42	102209.0
43	97250.0
44	92434.0
45	87538.0
46	82588.0
47	78750.5
48	75594.5
49	73275.5
50	72729.0
51	68473.0
52	59371.5
53	51744.5
54	46424.5
55	41839.0
56	37547.0
57	33210.5
58	29128.0
59	25058.5
60	21005.5
61	17295.0
62	14023.0
63	11105.5
64	8697.5
65	6900.0
66	5353.0
67	4197.5
68	3244.0
69	2373.5
70	1761.5
71	1362.5
72	1027.0
73	756.5
74	541.5
75	392.5
76	298.0
77	213.0
78	150.0
79	109.0
80	77.0
81	50.5
82	40.5
83	27.0
84	13.0
85	14.0
86	12.5
87	5.0
88	4.0
89	1.5
90	1.0
91	3.5
92	3.5
93	2.5
94	1.5
95	1.0
96	1.5
97	0.5
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	9.300389420486168
2	26.539722361538885
3	7.136623153196591
4	26.21571389028804
5	13.887451843006856
6	11.604529440971401
7	9.039315780271636
8	10.418389177075925
9	6.397668431450758
10-11	7.86651281799053
12-13	11.531362763474535
14-15	8.740438159976534
16-17	9.390052706574698
18-19	7.5580136719009445
20-21	9.349125320732485
22-23	6.741404620701862
24-25	5.998752073743969
26-27	11.04493719261828
28-29	7.420691522035627
30-31	5.021646637976809
32-33	9.996065226764642
34-35	8.438544837566745
36-37	8.293450074512974
38-39	7.228889277419823
40-41	10.128217601576207
42-43	7.4537565416502565
44-45	7.701690336936499
46-47	6.972787955572963
48-49	4.632208201201901
50-51	7.851416356809258
52-53	5.473068523573277
54-55	5.730372536144446
56-57	5.643778803994081
58-59	4.341013441056128
60-61	4.997844132000154
62-63	3.4733528612012328
64-65	4.88850697973484
66-67	2.9506311613217537
68-69	6.283161504131692
70-71	3.218526032509987
72-73	2.000846550665052
74-75	1.1074807004039964
76-77	1.396018770591591
78-79	2.2120534023402567
80-81	2.536582441218042
82-83	1.199585269156799
84-85	1.6661036159345393
86-87	0.5689086138145724
88-89	0.8157653726312827
90-91	0.3718109398902356
92-93	0.5335459163982752
94-95	0.7560975522192157
96-97	0.7526689861245391
98-99	0.5134053344180287
100	0.8235738870353889
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2785421.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.2834468871206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.66818357302041	94.03829365614456
2	1.9917280592507642	3.8354088561291753
3	0.20300127223929626	0.5863698664101058
4	0.05412703742968876	0.2084615093407451
5	0.029466539825264353	0.14185700111065322
6	0.012217610956926713	0.07058122173952536
7	0.008856190983994795	0.05968932159607616
8	0.00671887555779781	0.051753319832831865
9	0.004888953969026346	0.042365280582929314
>10	0.018565851303727295	0.30035787581504214
>50	7.807618921119625E-4	0.05084274988677104
>100	0.0010904154835482789	0.22014048400206576
>500	2.6240066125905086E-4	0.17302806223729003
>1k	1.12457426149115E-4	0.22085079517216266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.180243130212633E-5	0.0
5	0.0	0.0	0.0	7.180243130212633E-5	0.0
6	0.0	0.0	0.0	7.180243130212633E-5	0.0
7	0.0	0.0	0.0	1.4360486260425265E-4	0.0
8	0.0	0.0	0.0	1.4360486260425265E-4	0.0
9	0.0	0.0	0.0	1.7950607825531581E-4	0.0
10-11	0.0	0.0	0.0	1.974566860808474E-4	0.0
12-13	0.0	0.0	0.0	2.5130850955744214E-4	0.0
14-15	0.0	0.0	0.0	3.2311094085956847E-4	0.0
16-17	0.0	0.0	0.0	3.5901215651063163E-4	0.0
18-19	0.0	0.0	0.0	3.5901215651063163E-4	0.0
20-21	0.0	0.0	0.0	3.949133721616948E-4	0.0
22-23	0.0	1.795060782553158E-5	0.0	4.487651956382895E-4	0.0
24-25	0.0	3.590121565106316E-5	0.0	5.385182347659474E-4	0.0
26-27	0.0	3.590121565106316E-5	0.0	6.462218817191369E-4	0.0
28-29	0.0	3.590121565106316E-5	0.0	0.0010231846460553	0.0
30-31	0.0	3.590121565106316E-5	0.0	0.0028002948207829265	0.0
32-33	3.590121565106316E-5	3.590121565106316E-5	0.0	0.007467452855421138	0.0
34-35	3.590121565106316E-5	3.590121565106316E-5	0.0	0.014468189907378453	0.0
36-37	3.590121565106316E-5	3.590121565106316E-5	0.0	0.027069516600901622	0.0
38-39	3.590121565106316E-5	3.590121565106316E-5	0.0	0.04616896332726722	0.0
40-41	3.590121565106316E-5	3.590121565106316E-5	0.0	0.06939704985350509	0.0
42-43	3.590121565106316E-5	3.590121565106316E-5	0.0	0.09276874124234721	0.0
44-45	3.590121565106316E-5	3.590121565106316E-5	0.0	0.12068193641104882	0.0
46-47	3.590121565106316E-5	3.590121565106316E-5	0.0	0.15433932608392054	1.795060782553158E-5
48-49	3.590121565106316E-5	3.590121565106316E-5	0.0	0.18937891255935818	3.590121565106316E-5
50-51	3.590121565106316E-5	3.590121565106316E-5	0.0	0.2275957566199149	3.590121565106316E-5
52-53	3.590121565106316E-5	3.590121565106316E-5	0.0	0.268864204010812	3.590121565106316E-5
54-55	3.590121565106316E-5	3.590121565106316E-5	0.0	0.31318425473204947	3.590121565106316E-5
56-57	3.590121565106316E-5	3.590121565106316E-5	0.0	0.36917220053988253	3.590121565106316E-5
58-59	3.590121565106316E-5	3.590121565106316E-5	0.0	0.4285707618345665	3.590121565106316E-5
60-61	3.590121565106316E-5	7.180243130212633E-5	0.0	0.49053626004830153	3.590121565106316E-5
62-63	3.590121565106316E-5	7.180243130212633E-5	0.0	0.5547276336324024	3.590121565106316E-5
64-65	3.590121565106316E-5	7.180243130212633E-5	0.0	0.6292585573240095	3.590121565106316E-5
66-67	5.3851823476594744E-5	7.180243130212633E-5	0.0	0.7190115964516675	3.590121565106316E-5
68-69	7.180243130212633E-5	7.180243130212633E-5	0.0	0.8191580375103081	3.590121565106316E-5
70-71	7.180243130212633E-5	7.180243130212633E-5	0.0	0.9380987649622804	3.590121565106316E-5
72-73	7.180243130212633E-5	1.0770364695318949E-4	0.0	1.0631786002905845	3.590121565106316E-5
74-75	7.180243130212633E-5	1.0770364695318949E-4	0.0	1.1954207281412756	3.590121565106316E-5
76-77	7.180243130212633E-5	1.0770364695318949E-4	0.0	1.3417720337428345	3.590121565106316E-5
78-79	7.180243130212633E-5	1.0770364695318949E-4	0.0	1.5065047617577378	3.590121565106316E-5
80-81	7.180243130212633E-5	1.0770364695318949E-4	0.0	1.696081131003177	3.590121565106316E-5
82-83	7.180243130212633E-5	1.2565425477872107E-4	0.0	1.8964458155517603	3.590121565106316E-5
84-85	7.180243130212633E-5	1.4360486260425265E-4	0.0	2.1017289666445396	3.590121565106316E-5
86-87	7.180243130212633E-5	1.4360486260425265E-4	0.0	2.3263090211497652	3.590121565106316E-5
88	7.180243130212633E-5	1.4360486260425265E-4	0.0	2.5161726001204126	3.590121565106316E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAGC	210	0.0011551798	17.89464	2
ATAGCGC	160	0.009001262	17.726433	3
CTAGTAC	660	0.0	17.189268	3
GCACCGT	240	3.5675588E-5	16.873652	6
GTATTAG	1110	0.0	15.801019	1
TCTATAC	1185	0.0	15.796719	3
TATACAG	1635	0.0	15.123298	5
TATACTG	1425	0.0	14.873137	5
AGTACCG	240	6.974356E-4	14.718209	5
GTATATA	2515	0.0	14.196683	1
GTCTAGG	800	3.6379788E-12	14.093945	1
GTACATG	11395	0.0	14.072644	1
ACCGAGC	255	0.0010725489	13.904502	8
TACATGG	11490	0.0	13.572827	2
GTACTGT	1210	0.0	13.387361	6
AACACCG	415	1.5331316E-6	13.375584	5
GTATTGG	1095	0.0	13.157208	1
CATGGGG	3375	0.0	12.9422455	4
GTATAAT	1460	0.0	12.871182	1
CTATACA	1455	0.0	12.866003	4
>>END_MODULE