FastQCFastQC Report
Wed 25 May 2016
SRR1295100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2785421
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT74590.2677871675412801No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55210.19821061160951972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC1100.003049907321.3765583
CGTACAC2505.0198378E-616.9302333
GAGTACT37150.016.86828612-13
GTCTAGA9900.016.7419241
AACCGGT1700.001935162716.5681617
CCGTGCG1700.001935395116.5678639
GTATAGG7150.016.1565441
TCTGTCG5103.6379788E-1215.6477068
GTATCAA120200.014.9173051
CAACCGG2257.3840877E-414.6079376
AGTACTT44950.014.56776912-13
CTACACT14550.014.5451364
GTACATG140000.013.7403361
GTATAGA12900.013.6271481
GTATATA28250.013.5120671
TCGGACT2802.4857404E-413.4369354
AGACCGT2100.00787500613.4154526
CATGGGG34550.013.3397074
AACGCAG129450.013.2030036
TATCAAC133800.013.20194152
Produced by FastQC (version 0.11.5)