FastQCFastQC Report
Wed 25 May 2016
SRR1295099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2680714
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG78250.016.391391
GTATAGG7950.015.6637521
GTATATA21750.015.4585031
GTATTAC9700.015.4053811
TACATGG82700.015.3558412
GTATCAA58900.015.1165841
GTATAAG10050.014.86887551
ACATGGG85500.014.6693413
GTATTAT16750.014.4971541
GTCTAGG7351.6370905E-1114.4010611
TACACTG16050.014.3596075
TACTTCG2100.005357932814.21859557
CATGGGG32300.013.7575084
CAACGCA60150.013.6605425
GTGTAGG7804.5474735E-1113.5702311
TATACAG14950.013.405365
AACGCAG60950.013.39576156
CTAAGCG2250.00806295713.3675139
CTATACC8850.013.3472334
GTATTAG10850.013.1986961
Produced by FastQC (version 0.11.5)