##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295099_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2680714 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.020858995028938 27.0 27.0 33.0 6.0 33.0 2 22.09904973078068 27.0 2.0 33.0 2.0 33.0 3 24.509432188588562 27.0 15.0 33.0 15.0 33.0 4 24.598313732833866 33.0 6.0 37.0 2.0 37.0 5 26.506619132067055 33.0 15.0 37.0 2.0 37.0 6 27.094552048446793 33.0 15.0 37.0 2.0 37.0 7 27.056250312416765 33.0 15.0 37.0 6.0 37.0 8 27.80299726117743 33.0 15.0 37.0 6.0 37.0 9 28.84719667969056 33.0 22.0 37.0 15.0 37.0 10-11 28.35120438062397 33.0 18.5 37.0 10.5 37.0 12-13 27.187942839109283 33.0 15.0 37.0 4.0 37.0 14-15 28.890546511116067 33.0 22.0 37.0 6.0 40.0 16-17 29.273733602316398 33.0 22.0 37.0 6.0 40.0 18-19 29.419615632253198 33.0 22.0 38.5 6.0 40.0 20-21 28.937024800109224 33.0 22.0 38.5 6.0 40.0 22-23 29.658441556988176 33.0 27.0 37.0 6.0 40.0 24-25 29.79877357301077 33.0 27.0 37.0 10.5 40.0 26-27 28.352806192678518 33.0 18.5 37.0 4.0 40.0 28-29 29.07394130817387 33.0 22.0 37.0 6.0 40.0 30-31 29.753354143709473 33.0 27.0 37.0 10.5 40.0 32-33 28.58218892429405 33.0 22.0 37.0 6.0 40.0 34-35 28.19954478545641 33.0 22.0 37.0 6.0 40.0 36-37 28.51491897307956 33.0 22.0 37.0 6.0 40.0 38-39 28.609233957818702 33.0 22.0 37.0 6.0 40.0 40-41 27.648121172195168 33.0 15.0 37.0 6.0 40.0 42-43 28.269305304482316 33.0 22.0 37.0 6.0 40.0 44-45 28.129461777720415 33.0 22.0 37.0 6.0 40.0 46-47 27.844895427113823 33.0 22.0 37.0 6.0 40.0 48-49 27.775720759469305 33.0 18.5 37.0 6.0 40.0 50-51 27.74641028472265 33.0 22.0 37.0 6.0 40.0 52-53 27.936726931705508 33.0 22.0 37.0 6.0 40.0 54-55 27.91145567934513 33.0 22.0 37.0 6.0 40.0 56-57 27.90313103150877 33.0 22.0 37.0 6.0 40.0 58-59 27.78361548453136 33.0 22.0 37.0 6.0 40.0 60-61 27.76029874876619 33.0 22.0 37.0 6.0 40.0 62-63 27.73344601475577 33.0 22.0 37.0 6.0 40.0 64-65 27.29534892569666 33.0 22.0 37.0 6.0 40.0 66-67 27.501842792629127 33.0 22.0 37.0 6.0 40.0 68-69 27.10365447414383 33.0 22.0 37.0 6.0 37.0 70-71 27.23238920675611 33.0 22.0 37.0 6.0 37.0 72-73 27.290984976390618 33.0 22.0 37.0 10.5 37.0 74-75 27.389408381498363 30.0 22.0 37.0 6.0 37.0 76-77 27.14850744988089 27.0 22.0 37.0 6.0 37.0 78-79 26.722853127935316 27.0 22.0 35.0 6.0 37.0 80-81 26.53533069920924 27.0 22.0 33.0 6.0 37.0 82-83 26.64422314353564 27.0 22.0 33.0 10.5 37.0 84-85 26.599757378071665 27.0 22.0 33.0 6.0 37.0 86-87 26.39267467547825 27.0 22.0 33.0 10.5 37.0 88-89 26.338986553582366 27.0 22.0 33.0 6.0 37.0 90-91 26.47861390659354 27.0 22.0 33.0 15.0 37.0 92-93 26.39840411920108 27.0 22.0 33.0 6.0 37.0 94-95 26.263222223631466 27.0 22.0 33.0 10.5 37.0 96-97 26.34609380187517 27.0 22.0 33.0 10.5 37.0 98-99 26.406134895404733 27.0 22.0 33.0 10.5 37.0 100 26.0839798650658 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.291334388823671 1101 2 11.17928904494137 1101 3 8.348280373440627 1101 4 12.83375033024861 1101 5 10.793997579847453 1101 6 9.876102830175572 1101 7 9.454141704371022 1101 8 9.04267213186916 1101 9 7.281694521172891 1101 10-11 7.719020821387073 1101 12-13 9.0589330693889 1101 14-15 7.941345499309328 1101 16-17 8.076264635571828 1101 18-19 8.16689863707495 1101 20-21 7.297691111394407 1101 22-23 7.397020627113648 1101 24-25 7.310063385423984 1101 26-27 8.571214862427063 1101 28-29 8.344836572807601 1101 30-31 5.5564133687018895 1101 32-33 7.124289124005077 1101 34-35 8.837541934055022 1101 36-37 8.210234802694405 1101 38-39 8.144924186187428 1101 40-41 8.92603754048871 1101 42-43 8.636039163011421 1101 44-45 8.409176581444672 1101 46-47 7.438359390770202 1101 48-49 5.491743438240494 1101 50-51 6.762003229398484 1101 52-53 5.8834655658063895 1101 54-55 4.130535005202503 1101 56-57 6.196098787617892 1101 58-59 6.1952479607192075 1101 60-61 6.589404293159884 1101 62-63 6.122552547343918 1101 64-65 6.73902412884091 1101 66-67 5.842288989812651 1101 68-69 6.778547897435278 1101 70-71 6.3101287537035375 1101 72-73 5.6046906835797365 1101 74-75 4.949661646227117 1101 76-77 5.174462645422103 1101 78-79 5.068107265393746 1101 80-81 4.908870154897873 1101 82-83 4.510613152118722 1101 84-85 4.214619336480165 1101 86-87 4.020294285940402 1101 88-89 4.116814773925288 1101 90-91 3.852524588159497 1101 92-93 3.7346189319621175 1101 94-95 3.6547270701279118 1101 96-97 3.773697271973262 1101 98-99 3.4563812510973655 1101 100 3.8207063799497583 1102 1 6.607188923425447 1102 2 10.273794503270004 1102 3 7.968522561054204 1102 4 12.37826803781186 1102 5 10.405941947296139 1102 6 9.42917577806232 1102 7 6.603476515061779 1102 8 7.7260613767407875 1102 9 6.458742038711403 1102 10-11 7.237881699037825 1102 12-13 8.827333504377904 1102 14-15 6.004585702414737 1102 16-17 7.18504841213041 1102 18-19 7.226867681775225 1102 20-21 6.74797973436057 1102 22-23 6.287911545967241 1102 24-25 6.787640737928843 1102 26-27 8.359229842417967 1102 28-29 7.4702499619479354 1102 30-31 4.119637081297721 1102 32-33 4.410735823494168 1102 34-35 4.956991856312332 1102 36-37 5.065958733661827 1102 38-39 4.494800384212436 1102 40-41 6.177285412492079 1102 42-43 6.746669366007488 1102 44-45 6.247374374804231 1102 46-47 5.428065287788442 1102 48-49 3.6414263819111987 1102 50-51 4.775894185858856 1102 52-53 5.540652292081592 1102 54-55 5.256630032109371 1102 56-57 6.096282454047504 1102 58-59 5.868726980045118 1102 60-61 5.901012927050022 1102 62-63 4.158264623166865 1102 64-65 5.960787029796087 1102 66-67 3.6762817633048996 1102 68-69 5.913213213382026 1102 70-71 5.228395600546293 1102 72-73 4.869137331164268 1102 74-75 4.11635576703905 1102 76-77 3.281756569020761 1102 78-79 4.27037877451507 1102 80-81 4.344883523444601 1102 82-83 3.786277761706959 1102 84-85 2.9531779566071883 1102 86-87 3.192819859446683 1102 88-89 3.339719665196661 1102 90-91 3.267274120447798 1102 92-93 2.913320096174072 1102 94-95 2.8939376511116954 1102 96-97 3.146105062991108 1102 98-99 2.8827448480301463 1102 100 3.0917302177172665 1103 1 6.529363270230547 1103 2 10.809455164022612 1103 3 7.720574997413799 1103 4 11.793410507525305 1103 5 10.140314356261339 1103 6 8.745976620273392 1103 7 7.82322645547946 1103 8 7.80054399033849 1103 9 6.934169629109352 1103 10-11 7.193948924304554 1103 12-13 8.529975174965692 1103 14-15 8.274513079345105 1103 16-17 7.839370046917377 1103 18-19 7.025486517087245 1103 20-21 8.10795709063001 1103 22-23 6.616732860736526 1103 24-25 5.1456530105393234 1103 26-27 5.773275581063594 1103 28-29 6.69831904535021 1103 30-31 5.898268008207545 1103 32-33 7.71919048716698 1103 34-35 6.973023755938716 1103 36-37 4.027167643600553 1103 38-39 6.06749604875063 1103 40-41 6.542944031647043 1103 42-43 7.341542971885335 1103 44-45 5.570616659659397 1103 46-47 6.3180605285512215 1103 48-49 5.632420266426092 1103 50-51 6.315915776335679 1103 52-53 5.978341372559239 1103 54-55 4.799831438472541 1103 56-57 5.832257339579648 1103 58-59 5.368612132523193 1103 60-61 4.936857784734624 1103 62-63 2.6852247957241175 1103 64-65 4.94543034541924 1103 66-67 4.053285670386366 1103 68-69 5.010339924949964 1103 70-71 3.7214782035624694 1103 72-73 4.457231626521601 1103 74-75 3.2141282088755645 1103 76-77 3.4665221661776613 1103 78-79 2.9224525392627676 1103 80-81 4.043505163350865 1103 82-83 3.4728243374443544 1103 84-85 3.205300967090494 1103 86-87 2.7749573313486877 1103 88-89 3.0461649289473165 1103 90-91 2.651089486782915 1103 92-93 2.324080019967486 1103 94-95 2.840969643326435 1103 96-97 2.9052919155921657 1103 98-99 2.76915623987556 1103 100 2.9662308713469407 1104 1 6.3872484049720875 1104 2 9.600045069592607 1104 3 7.204986052380018 1104 4 12.014819620349268 1104 5 9.425722819638882 1104 6 7.944142677774192 1104 7 3.8718130419320573 1104 8 6.386229088145868 1104 9 6.444245839911726 1104 10-11 6.956084121945718 1104 12-13 7.238405026696082 1104 14-15 7.062909492021205 1104 16-17 7.080482484188163 1104 18-19 6.807092403501052 1104 20-21 7.428086304440413 1104 22-23 6.2710810222702165 1104 24-25 5.79075322235305 1104 26-27 7.880730829371494 1104 28-29 6.94706350057745 1104 30-31 6.120188312207965 1104 32-33 7.522104692072286 1104 34-35 7.838140705870618 1104 36-37 6.456985786208573 1104 38-39 7.1440308616815535 1104 40-41 6.9990566052520045 1104 42-43 7.143036202893672 1104 44-45 6.644291274608317 1104 46-47 6.660248394561997 1104 48-49 5.425319982727071 1104 50-51 5.589453716276761 1104 52-53 5.911832357771939 1104 54-55 4.835237361076871 1104 56-57 5.930687389614285 1104 58-59 3.8823284967139102 1104 60-61 4.304421282375422 1104 62-63 3.886058107367923 1104 64-65 2.9013939739408485 1104 66-67 3.2031340915746824 1104 68-69 4.135855175166547 1104 70-71 2.577991413724071 1104 72-73 2.416612316149422 1104 74-75 3.0758543110443917 1104 76-77 3.649821261042696 1104 78-79 4.1256240129484105 1104 80-81 3.079365915345374 1104 82-83 2.9436620551607326 1104 84-85 2.8952633946319786 1104 86-87 0.7070933098128478 1104 88-89 0.45727920053242244 1104 90-91 2.278929823354808 1104 92-93 2.432935513181146 1104 94-95 2.4557519858201857 1104 96-97 2.8177438423038765 1104 98-99 2.4530264509828292 1104 100 0.9407399753711516 1105 1 5.620637384927406 1105 2 -2.658326314864233 1105 3 0.5455238843034245 1105 4 8.286195016305182 1105 5 8.495925426426851 1105 6 7.771628539596673 1105 7 5.171968815863995 1105 8 5.730020874594633 1105 9 4.716318374551292 1105 10-11 4.857869252765621 1105 12-13 5.059248530639394 1105 14-15 5.728752030385799 1105 16-17 5.844633612359356 1105 18-19 6.009424665087771 1105 20-21 6.802789652115564 1105 22-23 2.084757323673557 1105 24-25 4.036668092173816 1105 26-27 6.804418809243529 1105 28-29 5.888558902866826 1105 30-31 5.144634294256441 1105 32-33 7.032626388305797 1105 34-35 6.114974138507538 1105 36-37 6.114796951959544 1105 38-39 4.129436711349644 1105 40-41 5.393480365261304 1105 42-43 5.489036098556252 1105 44-45 5.989819684130843 1105 46-47 5.416224869220361 1105 48-49 5.033451986032919 1105 50-51 5.7527861618268155 1105 52-53 5.490162574711395 1105 54-55 5.084695721753015 1105 56-57 5.073122743890153 1105 58-59 1.9023025221887124 1105 60-61 4.291667680675271 1105 62-63 -0.818498325903267 1105 64-65 -0.30995505393646283 1105 66-67 0.7756008660566778 1105 68-69 2.721476243636605 1105 70-71 2.820216537258659 1105 72-73 2.514175396812309 1105 74-75 2.5841818097195812 1105 76-77 1.7396133092502453 1105 78-79 3.109358541951927 1105 80-81 1.5232081773073354 1105 82-83 1.0083023336135 1105 84-85 1.4040811418773345 1105 86-87 -0.3618330856314529 1105 88-89 1.4899730553331807 1105 90-91 1.307380714753286 1105 92-93 1.6579146228168895 1105 94-95 1.0042662301656016 1105 96-97 1.8672077859470235 1105 98-99 1.899584094201952 1105 100 1.9508629013217913 1106 1 6.080545005852283 1106 2 10.241164260384323 1106 3 6.722634044588506 1106 4 11.625373908325134 1106 5 9.828123743239392 1106 6 9.24252820073745 1106 7 6.491714954974267 1106 8 7.4404659960539234 1106 9 6.845465634582059 1106 10-11 5.585360014768671 1106 12-13 7.903469436158975 1106 14-15 7.853272421060453 1106 16-17 7.677781353944805 1106 18-19 7.1964574099119005 1106 20-21 8.269511173441984 1106 22-23 6.149002702260148 1106 24-25 5.075661455055144 1106 26-27 7.75830552082072 1106 28-29 6.177911374600086 1106 30-31 5.452311334920431 1106 32-33 7.288973715525241 1106 34-35 6.633302016785112 1106 36-37 5.864836664121498 1106 38-39 7.17999829856247 1106 40-41 6.553235058585923 1106 42-43 6.150947815200318 1106 44-45 4.607901159176489 1106 46-47 6.017814750990816 1106 48-49 5.8183549515117825 1106 50-51 6.460641629950022 1106 52-53 5.501614479973917 1106 54-55 5.118297845602367 1106 56-57 4.955061214367291 1106 58-59 5.048919108599247 1106 60-61 4.938249518944094 1106 62-63 4.891770058546957 1106 64-65 5.090023784132185 1106 66-67 2.991634043242506 1106 68-69 3.3667933300447253 1106 70-71 2.9464610529107063 1106 72-73 2.8958833757548454 1106 74-75 2.8200511670819957 1106 76-77 3.5055553720680876 1106 78-79 4.066039013066966 1106 80-81 3.6762779453149683 1106 82-83 2.1627158753176197 1106 84-85 2.763998860782131 1106 86-87 3.3044204493323335 1106 88-89 3.342598986265859 1106 90-91 3.061159541623823 1106 92-93 2.8191587458194043 1106 94-95 1.621376501358526 1106 96-97 1.5820392230246227 1106 98-99 1.9763743569229426 1106 100 2.7665871107322495 1107 1 6.047527653502417 1107 2 10.270840399482555 1107 3 6.141889193833386 1107 4 11.298067548177759 1107 5 9.42798219186675 1107 6 7.929280514912964 1107 7 3.7534879281455034 1107 8 6.252270538366524 1107 9 5.7279861997692265 1107 10-11 4.58600237711774 1107 12-13 6.176580484851705 1107 14-15 6.1685964430354225 1107 16-17 6.943306427854477 1107 18-19 6.159507277331166 1107 20-21 5.5164043005871335 1107 22-23 5.723548738248603 1107 24-25 4.670203552894268 1107 26-27 7.1196579300475165 1107 28-29 6.388924389918209 1107 30-31 5.012760811203286 1107 32-33 5.0186263391422905 1107 34-35 6.708198221092054 1107 36-37 6.450501758303471 1107 38-39 6.475974804557712 1107 40-41 7.621757771174497 1107 42-43 6.172665369537189 1107 44-45 4.3006245233695175 1107 46-47 3.5186721490830593 1107 48-49 3.9982338136038003 1107 50-51 4.740406479964324 1107 52-53 3.9165818545060347 1107 54-55 5.348264833438144 1107 56-57 5.36627932353197 1107 58-59 3.071749727034682 1107 60-61 2.9976584442335152 1107 62-63 0.6947234171766254 1107 64-65 2.222764301716495 1107 66-67 4.148640842220857 1107 68-69 5.073400570954856 1107 70-71 4.204077100724476 1107 72-73 2.3361800846613043 1107 74-75 2.435779066541425 1107 76-77 3.226181622270637 1107 78-79 3.8745907514087925 1107 80-81 2.4667059922257586 1107 82-83 1.6934841450306592 1107 84-85 2.308015884108304 1107 86-87 2.420877702736199 1107 88-89 2.4751957445077686 1107 90-91 1.3831765171709804 1107 92-93 2.2193004178461315 1107 94-95 2.377053668188257 1107 96-97 2.390573255379465 1107 98-99 1.7599605631205328 1107 100 0.03702775272298453 1108 1 4.446453313332462 1108 2 -2.998063592080344 1108 3 0.17265128002456365 1108 4 6.7380936601348544 1108 5 7.147324019006366 1108 6 6.925396177297255 1108 7 4.4724112665909495 1108 8 5.086105430731621 1108 9 4.919922718346278 1108 10-11 3.4992690116662892 1108 12-13 2.715861714683623 1108 14-15 4.260976660977722 1108 16-17 4.705702760356385 1108 18-19 4.086322973510661 1108 20-21 4.739176805982481 1108 22-23 4.177958234835124 1108 24-25 3.082727983838325 1108 26-27 4.877574738331919 1108 28-29 6.296318820308464 1108 30-31 4.606740841986962 1108 32-33 4.6812522401719185 1108 34-35 3.135628800652743 1108 36-37 1.3251951611914734 1108 38-39 2.4991123971491653 1108 40-41 5.492135437765516 1108 42-43 3.144053640777706 1108 44-45 3.9820824759638036 1108 46-47 4.797287346647487 1108 48-49 4.915045747788927 1108 50-51 2.289062864007242 1108 52-53 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2212 30-31 -5.793757173620165 2212 32-33 -17.120176994667432 2212 34-35 -13.563057639989509 2212 36-37 -17.50535816794467 2212 38-39 -23.543976192992165 2212 40-41 -10.134344428801025 2212 42-43 -13.915080445015153 2212 44-45 -16.968241120401668 2212 46-47 -16.929930904541507 2212 48-49 -17.557508505908263 2212 50-51 -8.757399278294248 2212 52-53 -1.9579888605822866 2212 54-55 -23.393422250916075 2212 56-57 -23.331305555711992 2212 58-59 -12.359261844937558 2212 60-61 -6.120491396632612 2212 62-63 -2.9646112457667932 2212 64-65 -17.26743178510496 2212 66-67 -17.639422063369413 2212 68-69 -14.55785641976754 2212 70-71 -9.562204810559354 2212 72-73 -17.453454155608167 2212 74-75 -6.263387095923175 2212 76-77 -11.093641199555053 2212 78-79 -4.4514630665632815 2212 80-81 -2.271332544786663 2212 82-83 -2.6677671846507387 2212 84-85 -5.006475910163562 2212 86-87 -4.6759259913604865 2212 88-89 -3.292169060162781 2212 90-91 -3.8875664056719827 2212 92-93 -5.343196533407298 2212 94-95 -3.5606969173850302 2212 96-97 -5.096613130223432 2212 98-99 -3.559281820147774 2212 100 -3.408668829172125 2213 1 -20.98012372906671 2213 2 -16.856215953335084 2213 3 -19.807183259789294 2213 4 -18.860665388469076 2213 5 -21.008139614223555 2213 6 -21.554093653795473 2213 7 -10.459047993797075 2213 8 -10.353995739092516 2213 9 -4.106875558622583 2213 10-11 -17.493079080234534 2213 12-13 -16.11205948824883 2213 14-15 -23.439471460359748 2213 16-17 -17.78351616163529 2213 18-19 -18.404258580397666 2213 20-21 -17.53377986034807 2213 22-23 -19.52878074530034 2213 24-25 -7.286583059938778 2213 26-27 -23.10269068738335 2213 28-29 -24.000820097306153 2213 30-31 -19.37233140471053 2213 32-33 -23.21369777706352 2213 34-35 -17.703465366022588 2213 36-37 -23.612326383103596 2213 38-39 -11.357050088925703 2213 40-41 -17.010639753817625 2213 42-43 -23.134212474355007 2213 44-45 -17.003629031022236 2213 46-47 -16.833513447735044 2213 48-49 -6.014905769916695 2213 50-51 -23.29590783575146 2213 52-53 -17.685356954721264 2213 54-55 -17.546679793548343 2213 56-57 -17.410011283739976 2213 58-59 -17.917621762746627 2213 60-61 -19.275140214282708 2213 62-63 -17.502253714146054 2213 64-65 -17.575778473039207 2213 66-67 -8.628123750271996 2213 68-69 -22.724726835415463 2213 70-71 -17.40105598168391 2213 72-73 -9.411833103055361 2213 74-75 -4.088834532962334 2213 76-77 -3.008236744909201 2213 78-79 -4.445753506949732 2213 80-81 -3.2264350268715454 2213 82-83 -3.292494034507964 2213 84-85 -2.798433322261065 2213 86-87 -3.1098272649564365 2213 88-89 -2.6167516108398807 2213 90-91 -2.6901121275848254 2213 92-93 -3.5478691113780343 2213 94-95 -3.525288694229385 2213 96-97 -3.0883230768442296 2213 98-99 -3.4635791774869595 2213 100 -2.915514955304417 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 729.0 3 793.0 4 3871.0 5 12389.0 6 18820.0 7 23998.0 8 22861.0 9 23666.0 10 28252.0 11 35180.0 12 43732.0 13 58897.0 14 48646.0 15 52846.0 16 57607.0 17 59887.0 18 58722.0 19 59160.0 20 57768.0 21 55651.0 22 57792.0 23 61332.0 24 66794.0 25 72241.0 26 79178.0 27 84808.0 28 92410.0 29 95605.0 30 100113.0 31 107936.0 32 121040.0 33 145447.0 34 176751.0 35 207252.0 36 228669.0 37 197494.0 38 62263.0 39 114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.00578656032814 21.773982472322103 14.085364344319007 24.134866623030753 2 14.484313431892879 21.774798119412623 40.67344767222343 23.067440776471066 3 17.338276047483145 27.276042710649982 30.817711365856297 24.567969876010576 4 11.718670841559662 18.247794261587934 40.119747814427775 29.913787082424626 5 12.503800378500618 38.03000334774915 36.76622804320646 12.69996823054377 6 28.909493639137825 39.18520769447299 19.009540360496533 12.895758305892654 7 24.763084332855616 32.21667926838415 25.661894230710757 17.358342168049475 8 23.540086931433045 36.86836266347594 22.503373888004464 17.088176517086545 9 23.981108437068208 17.56700782982817 23.00096517054752 35.4509185625561 10-11 22.478021519007882 28.004560473452635 29.92667462272019 19.590743384819298 12-13 23.266682487390916 25.753658887913623 29.78703163488532 21.19262698981014 14-15 20.80960780117442 27.301050302121162 27.232740096882512 24.656601799821907 16-17 20.3326347832507 30.94852716272647 27.96526802498554 20.75357002903729 18-19 20.195286037889336 29.934716201565305 29.633838179525153 20.236159581020214 20-21 21.716197382368357 29.25497083288169 28.585095356929287 20.443736427820667 22-23 20.763625750077622 29.3645686142811 29.104041389710865 20.76776424593041 24-25 21.04610365270802 29.08217160107017 28.690599693951498 21.181125052270318 26-27 20.947150708431153 29.05915069516504 28.832009969483796 21.16168862692001 28-29 20.762532643893245 28.985086360064884 29.241952091860146 21.010428904181726 30-31 21.39205159433904 29.4174844014665 28.550368224478444 20.640095779716017 32-33 20.492064297689527 29.255657482841723 29.187159454078692 21.065118765390057 34-35 20.645206443416576 28.70337875736752 29.031876598722008 21.619538200493892 36-37 21.220959279664182 29.37998471332886 28.503313947655666 20.895742059351292 38-39 20.97492966962374 29.010064527933764 28.811777118587607 21.203228683854892 40-41 21.13484798816862 29.040129880142256 28.440442392475983 21.38457973921314 42-43 20.834357494211954 29.133504986357277 28.986345298128157 21.045792221302612 44-45 21.274168676248866 28.66095399932995 28.930232353717365 21.134644970703818 46-47 21.132583634459472 29.197305308157045 28.904575767464717 20.765535289918766 48-49 20.98581206270123 28.859483595780627 29.012030356960977 21.142673984557167 50-51 21.297503639282 28.89756666891012 28.691721809269872 21.113207882538017 52-53 20.70819655469305 28.94083246762512 28.946383424529955 21.404587553151877 54-55 21.21869348778973 28.956291789324638 28.628813168447493 21.19620155443814 56-57 21.292566394716335 28.934387735421545 28.552315288856413 21.22073058100571 58-59 20.972128956332583 28.465586678331817 28.9870901500826 21.575194215253006 60-61 21.36932683289932 28.92785273503698 28.502473479249208 21.200346952814495 62-63 20.866359989882667 29.040589823458735 28.932024855987798 21.161025330670796 64-65 20.91570126142461 28.55185107109432 28.950795718673334 21.581651948807743 66-67 21.040006482536327 28.801177124385962 28.633539194728762 21.525277198348945 68-69 21.48379902149168 28.729553062423598 28.59240306569656 21.194244850388166 70-71 21.59051607088894 28.782592675861707 28.347149125914772 21.279742127334586 72-73 21.164143648188105 28.930992871391414 28.821099014346252 21.083764466074225 74-75 21.46443327321335 28.494671593646093 28.476330860061893 21.564564273078663 76-77 21.448679000172756 28.91671677169233 28.441555159409422 21.193049068725486 78-79 21.424658033549104 28.70640679988048 28.66862884238846 21.200306324181955 80-81 21.567340872377265 28.61927230493358 28.449701993052006 21.36368482963715 82-83 21.070535017539214 28.84502038097615 28.873945429203363 21.21049917228127 84-85 21.299181769992494 28.425318766885997 28.502131656802497 21.77336780631901 86-87 21.484255865693218 28.828689761011077 28.45570278539405 21.23135158790166 88-89 21.284398090659153 28.41330712669429 28.74516320840134 21.55713157424522 90-91 21.61128221693327 28.41774844677537 28.44354517305865 21.527424163232713 92-93 21.168374504199583 28.734958093956415 28.714418024827292 21.38224937701671 94-95 21.165117009654296 28.32570461333596 28.660280485574823 21.84889789143492 96-97 21.325480103506887 28.52308002203376 28.61175520024217 21.539684674217185 98-99 21.318824194566083 28.36556868031939 28.606619326066397 21.70898779904813 100 21.51505327476391 28.19403379546474 28.352836092220635 21.938076837550717 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 323.0 1 378.5 2 370.0 3 291.0 4 346.5 5 512.5 6 745.5 7 1093.5 8 1568.0 9 2256.0 10 3083.5 11 3943.5 12 4939.5 13 5957.0 14 6853.0 15 7750.0 16 8673.0 17 9509.0 18 10394.5 19 11431.5 20 12492.0 21 13707.5 22 15317.0 23 17273.0 24 19827.0 25 23292.5 26 27973.0 27 34164.5 28 41441.5 29 49625.5 30 58205.0 31 66723.0 32 75159.5 33 82744.5 34 89788.0 35 95862.0 36 100140.0 37 103296.5 38 105594.5 39 106473.0 40 106000.5 41 103873.5 42 100611.0 43 96863.0 44 91720.5 45 86632.5 46 82140.0 47 77391.5 48 73340.5 49 70227.5 50 68115.0 51 63845.5 52 56850.0 53 50990.5 54 46335.0 55 42192.5 56 38307.0 57 34253.0 58 30364.5 59 26581.5 60 22605.5 61 18935.5 62 15589.0 63 12622.0 64 10190.0 65 8113.0 66 6302.0 67 4868.0 68 3736.5 69 2872.5 70 2222.0 71 1718.0 72 1329.0 73 991.0 74 728.5 75 535.0 76 407.5 77 290.0 78 196.0 79 146.5 80 102.5 81 62.5 82 45.5 83 31.5 84 22.0 85 17.5 86 11.5 87 8.0 88 3.5 89 4.0 90 4.0 91 2.0 92 2.0 93 1.5 94 0.5 95 0.0 96 0.0 97 1.0 98 1.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.071842054019937 2 25.011657342036486 3 6.2037949591041786 4 24.158041477009483 5 12.63999068904777 6 10.017517721025069 7 7.987386942433994 8 8.59013680683579 9 5.076483354807712 10-11 6.898180857786396 12-13 10.00727791178022 14-15 7.355894735507033 16-17 8.042931845769449 18-19 6.635265828432275 20-21 8.113323539922574 22-23 5.805990493577458 24-25 5.305582766382389 26-27 10.017965362959272 28-29 6.4238482732585425 30-31 4.376893618640406 32-33 8.86248962030265 34-35 7.257338902993754 36-37 6.855169928608571 38-39 6.217988938767806 40-41 8.792079274402267 42-43 6.5889721917369775 44-45 6.636795271707463 46-47 6.037048338614265 48-49 4.043251163682512 50-51 6.607549332006323 52-53 4.573277865523886 54-55 5.049289107304994 56-57 4.840426841505659 58-59 3.7018309301178713 60-61 4.180416113020636 62-63 3.1033150123437263 64-65 4.619776671439027 66-67 2.63454064849887 68-69 5.116118317731769 70-71 2.60426886269852 72-73 1.542462194773482 74-75 0.8466401115523701 76-77 1.1047802936083446 78-79 1.7496458033195634 80-81 2.2151747631414613 82-83 0.7606182531967229 84-85 1.1963230691524722 86-87 0.4371969557364195 88-89 0.6251319611118531 90-91 0.22217961334181865 92-93 0.29430591998997285 94-95 0.5521476740898134 96-97 0.5522595845733637 98-99 0.4701172896474596 100 0.35318948608467743 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2680714.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.90076197273146 #Duplication Level Percentage of deduplicated Percentage of total 1 97.9220548220011 94.8872172618749 2 1.7708638183330812 3.4319610669283254 3 0.17770289032362793 0.5165863643134883 4 0.05431901443231893 0.2105421555239801 5 0.02700565693216337 0.1308434367150404 6 0.013153511081465659 0.07647511478464737 7 0.008038405605382753 0.05452493397452253 8 0.006139813596880749 0.047596209272662525 9 0.003969590693413196 0.0346190726620442 >10 0.015054173459009643 0.24465916125964207 >50 6.12661549717792E-4 0.04662810959489547 >100 0.0010084392728139462 0.21884978253341342 >500 0.0 0.0 >1k 7.720271900470847E-5 0.09949733056237493 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 7.460698903351868E-5 0.0 8 0.0 0.0 0.0 1.1191048355027803E-4 0.0 9 0.0 0.0 0.0 1.1191048355027803E-4 0.0 10-11 0.0 0.0 0.0 1.305622308086577E-4 0.0 12-13 0.0 0.0 0.0 1.6786572532541703E-4 0.0 14-15 0.0 0.0 0.0 2.2382096710055606E-4 0.0 16-17 3.730349451675934E-5 0.0 0.0 2.2382096710055606E-4 0.0 18-19 3.730349451675934E-5 1.865174725837967E-5 0.0 2.797762088756951E-4 0.0 20-21 3.730349451675934E-5 3.730349451675934E-5 0.0 3.5438319790921376E-4 0.0 22-23 3.730349451675934E-5 3.730349451675934E-5 0.0 3.730349451675934E-4 0.0 24-25 3.730349451675934E-5 1.1191048355027803E-4 0.0 3.9168669242597303E-4 0.0 26-27 3.730349451675934E-5 1.1191048355027803E-4 0.0 4.476419342011121E-4 0.0 28-29 3.730349451675934E-5 1.1191048355027803E-4 0.0 7.647216375935665E-4 0.0 30-31 3.730349451675934E-5 1.1191048355027803E-4 0.0 0.0026858516052066725 0.0 32-33 3.730349451675934E-5 1.1191048355027803E-4 0.0 0.007703171617710804 0.0 34-35 3.730349451675934E-5 1.1191048355027803E-4 0.0 0.016432189334632488 0.0 36-37 3.730349451675934E-5 1.305622308086577E-4 0.0 0.028947511745005247 0.0 38-39 3.730349451675934E-5 1.4921397806703736E-4 0.0 0.04815881142113631 0.0 40-41 3.730349451675934E-5 1.4921397806703736E-4 1.865174725837967E-5 0.06912337533955507 0.0 42-43 3.730349451675934E-5 1.4921397806703736E-4 3.730349451675934E-5 0.090834009148309 0.0 44-45 3.730349451675934E-5 1.4921397806703736E-4 3.730349451675934E-5 0.11849455033248604 0.0 46-47 3.730349451675934E-5 1.4921397806703736E-4 3.730349451675934E-5 0.15342927294743117 0.0 48-49 3.730349451675934E-5 1.4921397806703736E-4 3.730349451675934E-5 0.18903545846367797 0.0 50-51 3.730349451675934E-5 1.4921397806703736E-4 3.730349451675934E-5 0.22708502287077248 0.0 52-53 3.730349451675934E-5 1.4921397806703736E-4 3.730349451675934E-5 0.2647055970909243 0.0 54-55 3.730349451675934E-5 1.4921397806703736E-4 3.730349451675934E-5 0.3074554018071305 1.865174725837967E-5 56-57 3.730349451675934E-5 1.4921397806703736E-4 3.730349451675934E-5 0.3614522101201396 3.730349451675934E-5 58-59 3.730349451675934E-5 1.6786572532541703E-4 3.730349451675934E-5 0.42069015941275345 3.730349451675934E-5 60-61 3.730349451675934E-5 2.2382096710055606E-4 3.730349451675934E-5 0.48546767764110604 3.730349451675934E-5 62-63 7.460698903351868E-5 2.2382096710055606E-4 3.730349451675934E-5 0.55080474828721 3.730349451675934E-5 64-65 7.460698903351868E-5 2.2382096710055606E-4 3.730349451675934E-5 0.6223341990230962 3.730349451675934E-5 66-67 7.460698903351868E-5 2.4247271435893573E-4 3.730349451675934E-5 0.7138769745672235 3.730349451675934E-5 68-69 7.460698903351868E-5 2.611244616173154E-4 3.730349451675934E-5 0.8148015789823159 3.730349451675934E-5 70-71 7.460698903351868E-5 2.611244616173154E-4 3.730349451675934E-5 0.9302185910171693 3.730349451675934E-5 72-73 7.460698903351868E-5 2.611244616173154E-4 3.730349451675934E-5 1.0489183105694975 3.730349451675934E-5 74-75 7.460698903351868E-5 2.984279561340747E-4 3.730349451675934E-5 1.1771490729708578 3.730349451675934E-5 76-77 7.460698903351868E-5 2.984279561340747E-4 3.730349451675934E-5 1.3226700050807358 3.730349451675934E-5 78-79 7.460698903351868E-5 3.1707970339245437E-4 3.730349451675934E-5 1.4892860633398417 5.5955241775139015E-5 80-81 7.460698903351868E-5 3.3573145065083406E-4 3.730349451675934E-5 1.6714390270651773 7.460698903351868E-5 82-83 7.460698903351868E-5 3.3573145065083406E-4 3.730349451675934E-5 1.8737358778295632 7.460698903351868E-5 84-85 7.460698903351868E-5 3.3573145065083406E-4 3.730349451675934E-5 2.0810500486064534 7.460698903351868E-5 86-87 7.460698903351868E-5 3.3573145065083406E-4 3.730349451675934E-5 2.3088438378730443 7.460698903351868E-5 88 7.460698903351868E-5 3.3573145065083406E-4 3.730349451675934E-5 2.5004532374583786 7.460698903351868E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7825 0.0 16.39139 1 GTATAGG 795 0.0 15.663752 1 GTATATA 2175 0.0 15.458503 1 GTATTAC 970 0.0 15.405381 1 TACATGG 8270 0.0 15.355841 2 GTATCAA 5890 0.0 15.116584 1 GTATAAG 1005 0.0 14.8688755 1 ACATGGG 8550 0.0 14.669341 3 GTATTAT 1675 0.0 14.497154 1 GTCTAGG 735 1.6370905E-11 14.401061 1 TACACTG 1605 0.0 14.359607 5 TACTTCG 210 0.0053579328 14.2185955 7 CATGGGG 3230 0.0 13.757508 4 CAACGCA 6015 0.0 13.660542 5 GTGTAGG 780 4.5474735E-11 13.570231 1 TATACAG 1495 0.0 13.40536 5 AACGCAG 6095 0.0 13.3957615 6 CTAAGCG 225 0.008062957 13.367513 9 CTATACC 885 0.0 13.347233 4 GTATTAG 1085 0.0 13.198696 1 >>END_MODULE