FastQCFastQC Report
Wed 25 May 2016
SRR1295099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2680714
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42200.1574207468607244No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31390.11709566928810758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1656.8380075E-519.9489233
GTATCAA83400.018.974191
GAGTACT35200.017.07354512-13
CAACGCA91400.017.0295565
TCAACGC91950.016.9788364
AACGCAG91600.016.918276
ATCAACG93000.016.8876343
TATCAAC95950.016.428532
CGCAGAG99200.015.5721568
ACGCAGA99950.015.5492617
ATACCGT2458.087841E-515.3342096
CCTAGAC9650.015.105663
GTATAGG7250.014.4139641
GTATTAC10400.014.3545671
AGTACTT41350.014.30709612-13
GTACATG100300.014.2391191
TACATGG101800.014.1167422
GTACTAT9550.014.0689781
ACATGGG99850.014.0337433
GTACTAG7500.013.9334991
Produced by FastQC (version 0.11.5)