##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295099_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2680714 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.125050639493807 33.0 33.0 33.0 27.0 33.0 2 29.82715239298187 33.0 33.0 33.0 22.0 33.0 3 30.66660039079141 33.0 33.0 33.0 27.0 33.0 4 34.936290107784714 37.0 37.0 37.0 33.0 37.0 5 35.24132376672782 37.0 37.0 37.0 33.0 37.0 6 34.907247472128695 37.0 37.0 37.0 33.0 37.0 7 34.80063184659012 37.0 37.0 37.0 27.0 37.0 8 34.785209089817116 37.0 37.0 37.0 27.0 37.0 9 34.7649581417488 37.0 37.0 37.0 27.0 37.0 10-11 34.744855102036254 37.0 37.0 37.0 27.0 37.0 12-13 34.64061440347609 37.0 37.0 37.0 27.0 37.0 14-15 36.010939063249566 37.0 37.0 40.0 27.0 40.0 16-17 36.007283880339344 37.0 37.0 40.0 27.0 40.0 18-19 36.04205073722896 37.0 37.0 40.0 27.0 40.0 20-21 36.044552682606195 37.0 37.0 40.0 27.0 40.0 22-23 36.00934508492887 37.0 37.0 40.0 27.0 40.0 24-25 35.97510178258479 37.0 37.0 40.0 27.0 40.0 26-27 35.95045834803713 37.0 37.0 40.0 27.0 40.0 28-29 35.89278304212982 37.0 37.0 40.0 27.0 40.0 30-31 35.794557159025544 37.0 37.0 40.0 27.0 40.0 32-33 35.756671170441905 37.0 37.0 40.0 27.0 40.0 34-35 35.64036987906953 37.0 37.0 40.0 27.0 40.0 36-37 35.63125738142898 37.0 37.0 40.0 27.0 40.0 38-39 35.568253457847426 37.0 37.0 40.0 27.0 40.0 40-41 35.51291502935412 37.0 37.0 40.0 27.0 40.0 42-43 35.42682658426076 37.0 37.0 40.0 27.0 40.0 44-45 35.32346904593328 37.0 33.0 40.0 27.0 40.0 46-47 35.21530644447711 37.0 33.0 40.0 27.0 40.0 48-49 35.110955141055705 37.0 33.0 40.0 27.0 40.0 50-51 35.01383530656385 37.0 33.0 40.0 24.5 40.0 52-53 34.92364235796881 37.0 33.0 40.0 22.0 40.0 54-55 34.80532220147319 37.0 33.0 40.0 22.0 40.0 56-57 34.68570052605388 37.0 33.0 40.0 22.0 40.0 58-59 34.564158093701906 37.0 33.0 40.0 22.0 40.0 60-61 34.412960129278986 37.0 33.0 40.0 22.0 40.0 62-63 34.236883718292965 37.0 33.0 40.0 22.0 40.0 64-65 34.0662868176165 37.0 33.0 40.0 22.0 40.0 66-67 33.851013759766985 37.0 33.0 40.0 22.0 40.0 68-69 33.646014270824864 37.0 33.0 37.0 22.0 40.0 70-71 33.42478589659322 37.0 33.0 37.0 22.0 40.0 72-73 33.215789338213625 37.0 33.0 37.0 22.0 40.0 74-75 32.98278201255337 37.0 33.0 37.0 22.0 40.0 76-77 32.74756967733224 37.0 33.0 37.0 22.0 38.5 78-79 32.514683214994214 37.0 33.0 37.0 22.0 37.0 80-81 32.294180580248394 37.0 33.0 37.0 22.0 37.0 82-83 32.077274748443884 35.0 33.0 37.0 22.0 37.0 84-85 31.904580831823164 33.0 33.0 37.0 22.0 37.0 86-87 31.916307931394396 33.0 33.0 37.0 22.0 37.0 88-89 31.916802202696744 33.0 33.0 37.0 22.0 37.0 90-91 31.913719814944823 33.0 33.0 37.0 22.0 37.0 92-93 31.8720307350952 33.0 33.0 37.0 22.0 37.0 94-95 31.887479604314372 33.0 33.0 37.0 22.0 37.0 96-97 31.84296086788818 33.0 33.0 37.0 22.0 37.0 98-99 31.76323826413411 33.0 33.0 37.0 22.0 37.0 100 31.22767441808414 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6465053998300547 1101 2 4.08984423754309 1101 3 2.356693358391457 1101 4 1.1448994820975784 1101 5 0.6407246494335084 1101 6 0.6706779264897094 1101 7 0.6795547135590354 1101 8 0.6514593425833013 1101 9 0.7148970201685998 1101 10-11 0.7846882416565037 1101 12-13 0.7913525048900993 1101 14-15 1.070555952189956 1101 16-17 1.1537548234902957 1101 18-19 1.1719382246922265 1101 20-21 1.1165109557756878 1101 22-23 1.0661134848697884 1101 24-25 1.0063193336836846 1101 26-27 1.1634708220283159 1101 28-29 0.9681625468784816 1101 30-31 0.8389429640351693 1101 32-33 1.092097083314627 1101 34-35 1.2298174602292775 1101 36-37 1.0807470324905708 1101 38-39 1.1063543337163964 1101 40-41 1.0331756897653506 1101 42-43 1.1959947940252036 1101 44-45 1.2499946430774926 1101 46-47 1.1829123803002588 1101 48-49 1.1502610959049164 1101 50-51 1.1634309942041199 1101 52-53 1.2266504555088176 1101 54-55 1.1748608016969655 1101 56-57 1.1046299585788901 1101 58-59 1.092408245901133 1101 60-61 1.0665880049255634 1101 62-63 1.0787191514461298 1101 64-65 1.1390393022362204 1101 66-67 1.0500882820686073 1101 68-69 1.0174048205324056 1101 70-71 1.0433169711439305 1101 72-73 1.0148175214524784 1101 74-75 1.019991972660435 1101 76-77 1.0499837316974308 1101 78-79 1.0945112860332031 1101 80-81 1.0878367344266096 1101 82-83 1.042448244414139 1101 84-85 1.0385766373138878 1101 86-87 1.106260883234036 1101 88-89 0.9586797097156641 1101 90-91 1.0042178263599304 1101 92-93 1.0597089224884328 1101 94-95 1.0331886752332373 1101 96-97 1.0248830215174465 1101 98-99 1.02761686331095 1101 100 1.0939483930078922 1102 1 0.46455740747299856 1102 2 3.927234776904708 1102 3 2.180583246723373 1102 4 1.0289619482680052 1102 5 0.47869551055289605 1102 6 0.5482810390328439 1102 7 0.4490869561998494 1102 8 0.49855363069130476 1102 9 0.4980580532086165 1102 10-11 0.6115457008156184 1102 12-13 0.5601820836137534 1102 14-15 0.8528190484480334 1102 16-17 0.8144129071621222 1102 18-19 0.8429629215281764 1102 20-21 0.7465661680442892 1102 22-23 0.7950332069064743 1102 24-25 0.7693055348818447 1102 26-27 0.8334594102352355 1102 28-29 0.7796744304382983 1102 30-31 0.8254265133551257 1102 32-33 0.8774599748500904 1102 34-35 0.9764210460410183 1102 36-37 0.8468035322804823 1102 38-39 0.9433606652310331 1102 40-41 0.9254647274873378 1102 42-43 0.8792539771364218 1102 44-45 1.0093213629054816 1102 46-47 0.8989192824045418 1102 48-49 0.8070249862906707 1102 50-51 0.8204011210903275 1102 52-53 0.8164628169389729 1102 54-55 0.7385580232053002 1102 56-57 0.8098787706314141 1102 58-59 0.7706391452352577 1102 60-61 0.7884394860496684 1102 62-63 0.8952449008511891 1102 64-65 0.7689027259735965 1102 66-67 0.83418849318317 1102 68-69 0.765452512407748 1102 70-71 0.7799444895210925 1102 72-73 0.7626105866435111 1102 74-75 0.7124214710762047 1102 76-77 0.7040163551518717 1102 78-79 0.6588795421206655 1102 80-81 0.7054100427015726 1102 82-83 0.7823365583078896 1102 84-85 0.7289377632534482 1102 86-87 0.7983472348712866 1102 88-89 0.7545230259649536 1102 90-91 0.7918564382543245 1102 92-93 0.822925059662559 1102 94-95 0.7713246697323193 1102 96-97 0.7960601098857936 1102 98-99 0.8633752026727812 1102 100 0.8211682709144412 1103 1 0.5430270151016927 1103 2 3.935917016866771 1103 3 2.2815847073168634 1103 4 1.0715414450429606 1103 5 0.4457043581292055 1103 6 0.46239089143770684 1103 7 0.3912700621569343 1103 8 0.4891783252783455 1103 9 0.5775100648865035 1103 10-11 0.5851425280234608 1103 12-13 0.5883616116923918 1103 14-15 0.8039834082032584 1103 16-17 0.7588177661400195 1103 18-19 0.8270802226414133 1103 20-21 0.7973520729129433 1103 22-23 0.747828364731653 1103 24-25 0.8062568294898966 1103 26-27 0.8605028327024087 1103 28-29 0.9147736640859847 1103 30-31 0.7807710963364585 1103 32-33 0.8951686911615795 1103 34-35 1.0116473620999287 1103 36-37 0.8849721003967304 1103 38-39 0.9084028676384648 1103 40-41 0.8061547470754036 1103 42-43 0.8769053131642934 1103 44-45 1.0096761012123423 1103 46-47 0.959732468994325 1103 48-49 0.9195157305173112 1103 50-51 0.7981881223226424 1103 52-53 0.8234907749469684 1103 54-55 0.7850275166906826 1103 56-57 0.7407062092138617 1103 58-59 0.8050663050238924 1103 60-61 0.7110237751291208 1103 62-63 0.765869764448702 1103 64-65 0.7132887855970651 1103 66-67 0.7099600627786273 1103 68-69 0.8004219447799201 1103 70-71 0.7974910231283374 1103 72-73 0.741830288513917 1103 74-75 0.6821511731009267 1103 76-77 0.6495678828964131 1103 78-79 0.6630283820596574 1103 80-81 0.7667224531903258 1103 82-83 0.7358795569016721 1103 84-85 0.7223299738787219 1103 86-87 0.7960230039363481 1103 88-89 0.8264364965168305 1103 90-91 0.6672556949021278 1103 92-93 0.7448914194731415 1103 94-95 0.6596948379614354 1103 96-97 0.6052467849033789 1103 98-99 0.7430973310683306 1103 100 0.8023786529589643 1104 1 0.36315464867509917 1104 2 3.557042740522359 1104 3 2.0588411308253356 1104 4 0.8914136379533417 1104 5 0.3506247203814894 1104 6 0.4163421656044832 1104 7 0.3567152985789761 1104 8 0.24517287686905576 1104 9 0.32526600440866815 1104 10-11 0.5003713029617742 1104 12-13 0.4224091191938726 1104 14-15 0.556993852640268 1104 16-17 0.5338997835387431 1104 18-19 0.5573847683945417 1104 20-21 0.5638155706776828 1104 22-23 0.5228304717143146 1104 24-25 0.6421382175204968 1104 26-27 0.5459941810597684 1104 28-29 0.5394204966093668 1104 30-31 0.4294478802381221 1104 32-33 0.6641606300937895 1104 34-35 0.6785319155621394 1104 36-37 0.5141119185269432 1104 38-39 0.5428291672439727 1104 40-41 0.5205930007090274 1104 42-43 0.6564870709083763 1104 44-45 0.6050437139142275 1104 46-47 0.6647275361760805 1104 48-49 0.5732093950683108 1104 50-51 0.3988357716116582 1104 52-53 0.4473661729497991 1104 54-55 0.5847519357521875 1104 56-57 0.5873204739822597 1104 58-59 0.5721506810037909 1104 60-61 0.5966857271122308 1104 62-63 0.5519490539245737 1104 64-65 0.5938635828619496 1104 66-67 0.53107385719413 1104 68-69 0.5766676363102192 1104 70-71 0.4666345098871787 1104 72-73 0.4720111729756624 1104 74-75 0.4548442129935495 1104 76-77 0.5750630238225654 1104 78-79 0.45913195141222474 1104 80-81 0.5458721865330745 1104 82-83 0.48109255425803354 1104 84-85 0.5184723674853942 1104 86-87 0.5889859607023027 1104 88-89 0.5710652399743417 1104 90-91 0.5414865842595056 1104 92-93 0.5694039008666252 1104 94-95 0.526608021599845 1104 96-97 0.5511117074841607 1104 98-99 0.4953272313832606 1104 100 0.3913138334963904 1105 1 0.37834956286672394 1105 2 3.639643378078887 1105 3 2.0650296209439425 1105 4 0.877598082708964 1105 5 0.2651051978441501 1105 6 0.25433132732656816 1105 7 0.20075859311526756 1105 8 0.169152452362205 1105 9 0.2219874299140372 1105 10-11 0.22092513328899344 1105 12-13 0.2569751441854322 1105 14-15 0.3974191099830122 1105 16-17 0.3645273914021203 1105 18-19 0.39922814511943017 1105 20-21 0.3418947371498575 1105 22-23 0.27583300967620517 1105 24-25 0.29832469411001483 1105 26-27 0.2363815016840789 1105 28-29 0.29540118137399674 1105 30-31 0.3266324174330393 1105 32-33 0.34550441364307716 1105 34-35 0.504508362763282 1105 36-37 0.29282992286335485 1105 38-39 0.37725818073369766 1105 40-41 0.33858090855162715 1105 42-43 0.420764959887876 1105 44-45 0.3659343893525602 1105 46-47 0.3815990794271471 1105 48-49 0.3467661752156488 1105 50-51 0.379179746446205 1105 52-53 0.2675257634345911 1105 54-55 0.2943453950775279 1105 56-57 0.3039387701888643 1105 58-59 0.2847191753142013 1105 60-61 0.29695110109322087 1105 62-63 0.37579340263486216 1105 64-65 0.27452024961099397 1105 66-67 0.23958568530464674 1105 68-69 0.25950921092657353 1105 70-71 0.24521257395218754 1105 72-73 0.3565581609649655 1105 74-75 0.3972241494575499 1105 76-77 0.27438025441465896 1105 78-79 0.31808378138983784 1105 80-81 0.31887739699719475 1105 82-83 0.2971945633024333 1105 84-85 0.27285334915404036 1105 86-87 0.35650014006564845 1105 88-89 0.34568841258229455 1105 90-91 0.3586693092782518 1105 92-93 0.29802603376137426 1105 94-95 0.3312175388196401 1105 96-97 0.2483934889814705 1105 98-99 0.30461724272309354 1105 100 0.12552689599175793 1106 1 0.5403351137218166 1106 2 3.8947391013831485 1106 3 2.204849166733407 1106 4 0.9508284721010227 1106 5 0.31032134022978397 1106 6 0.23686200094679322 1106 7 0.2502200013908151 1106 8 0.29089402028064626 1106 9 0.21317347656295027 1106 10-11 0.27064136127043525 1106 12-13 0.301004349081758 1106 14-15 0.328398075595679 1106 16-17 0.31800852910905064 1106 18-19 0.4035824852920129 1106 20-21 0.36720037321794763 1106 22-23 0.20916760413900448 1106 24-25 0.2505454626047552 1106 26-27 0.35788654074599435 1106 28-29 0.28736282264545565 1106 30-31 0.31739866896450053 1106 32-33 0.41457209193234945 1106 34-35 0.44396775323168214 1106 36-37 0.28912345441404597 1106 38-39 0.2574229307519005 1106 40-41 0.2989461137570757 1106 42-43 0.2783801783754214 1106 44-45 0.3067854048837759 1106 46-47 0.23039600663600623 1106 48-49 0.221701978359242 1106 50-51 0.19411007038475248 1106 52-53 0.18063184123786868 1106 54-55 0.1830476338238185 1106 56-57 0.2463914201438655 1106 58-59 0.24606215834511858 1106 60-61 0.232306722609124 1106 62-63 0.18256265368674462 1106 64-65 0.1648062791407412 1106 66-67 -0.009804858227845159 1106 68-69 0.04308562025664031 1106 70-71 0.0991597839670888 1106 72-73 0.19389370207984058 1106 74-75 0.1204745648700225 1106 76-77 0.23207611363469027 1106 78-79 0.21619346459232247 1106 80-81 0.2360614845127884 1106 82-83 0.27348926475195157 1106 84-85 0.3191695363142415 1106 86-87 0.2937824275291021 1106 88-89 0.27426054391077415 1106 90-91 0.24930572708382215 1106 92-93 0.3216875505477219 1106 94-95 0.2640573412104885 1106 96-97 0.28755106701900246 1106 98-99 0.2871825303223261 1106 100 0.3061166108638034 1107 1 0.48420242945919867 1107 2 3.5661615297951563 1107 3 2.0425150676085977 1107 4 0.9242408966002245 1107 5 0.2987147792054188 1107 6 0.22299576988963565 1107 7 0.06556574110110347 1107 8 0.14100032256232709 1107 9 0.14160243851431886 1107 10-11 0.12069605376721171 1107 12-13 0.08459028380765687 1107 14-15 0.027019946537556905 1107 16-17 0.007817584367046493 1107 18-19 0.09181127367042308 1107 20-21 0.11038181874317132 1107 22-23 0.10331279829978257 1107 24-25 0.08137977836397425 1107 26-27 0.16970200225330245 1107 28-29 -0.021128950616081 1107 30-31 -0.023656163852969314 1107 32-33 0.0910587342582545 1107 34-35 0.1562508941036569 1107 36-37 0.09686871068753788 1107 38-39 0.04636276985002752 1107 40-41 0.008838524096312028 1107 42-43 0.14891641459778526 1107 44-45 0.22078314286098077 1107 46-47 0.06468136102781585 1107 48-49 0.010267729466512776 1107 50-51 0.16208868734386783 1107 52-53 0.016722572868559382 1107 54-55 -0.022014390218430435 1107 56-57 0.030030171195519983 1107 58-59 0.08221569042710541 1107 60-61 0.03317931105068084 1107 62-63 0.14553907293169743 1107 64-65 0.05335544634402112 1107 66-67 -0.05224070976878892 1107 68-69 0.04161671709758963 1107 70-71 0.006706359158663133 1107 72-73 0.010096719228286588 1107 74-75 0.045877190568347714 1107 76-77 0.002812991567864742 1107 78-79 0.08464069598526436 1107 80-81 0.17068132822472393 1107 82-83 0.1306366981318945 1107 84-85 0.28714842474504465 1107 86-87 0.1319348056754137 1107 88-89 0.004762542123405211 1107 90-91 0.06165731501826954 1107 92-93 0.14890161031999583 1107 94-95 0.05129481149489479 1107 96-97 -0.0020430490191003514 1107 98-99 0.06272569389897953 1107 100 0.10387279477778932 1108 1 0.4045198775963428 1108 2 3.5986343099319242 1108 3 2.011984637260813 1108 4 0.9785775241520369 1108 5 0.32428204343557354 1108 6 0.1822256686475896 1108 7 0.07459090797475199 1108 8 0.09837419952918225 1108 9 0.031167271805969676 1108 10-11 0.08432629749932374 1108 12-13 0.04230045387321013 1108 14-15 -0.0054523208193515416 1108 16-17 0.009063337795709003 1108 18-19 0.12376518091421218 1108 20-21 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0.41901507633932766 2210 48-49 0.25983682152624965 2210 50-51 0.20827119573634434 2210 52-53 0.2067130904888046 2210 54-55 0.12447975881334372 2210 56-57 0.019020252857181674 2210 58-59 0.12802172557540104 2210 60-61 0.19587086867024794 2210 62-63 0.23797083034182975 2210 64-65 -0.2281786773159027 2210 66-67 0.16315597967173545 2210 68-69 0.25916441880699637 2210 70-71 0.2237161274207793 2210 72-73 0.14404539444491604 2210 74-75 -0.5144631088561482 2210 76-77 -3.6732915610571126E-5 2210 78-79 0.07677299843180663 2210 80-81 0.13880151618556624 2210 82-83 0.24151508332462868 2210 84-85 0.18021627442703902 2210 86-87 0.3641377064755176 2210 88-89 0.3692324548082233 2210 90-91 0.2929168280626335 2210 92-93 0.31794695562436814 2210 94-95 0.20730881924979627 2210 96-97 0.21089530060108075 2210 98-99 0.25896714248080244 2210 100 0.06571559603374766 2211 1 0.5811687657410012 2211 2 4.018030549212575 2211 3 2.2423228515791216 2211 4 1.082287329191729 2211 5 0.5581378707101052 2211 6 0.37393500904707366 2211 7 0.27094891325022985 2211 8 0.2859568409936415 2211 9 0.2176818442351518 2211 10-11 0.25703272107467967 2211 12-13 0.19493004587234708 2211 14-15 0.10975223778377341 2211 16-17 0.2022459874064566 2211 18-19 0.32416238794385777 2211 20-21 0.2909150886597658 2211 22-23 0.2486363755654466 2211 24-25 0.17244668583042966 2211 26-27 0.21820521151296646 2211 28-29 0.18132170802189052 2211 30-31 0.11443971796518326 2211 32-33 0.33697736680549184 2211 34-35 0.4240119845651762 2211 36-37 0.2571153262276553 2211 38-39 -0.061534464909598796 2211 40-41 0.09009562305628549 2211 42-43 0.3051307442785358 2211 44-45 0.28628862455374104 2211 46-47 0.22594477613147745 2211 48-49 0.21162301969638264 2211 50-51 -0.015718352169308503 2211 52-53 0.1483008786255624 2211 54-55 0.21605820815984345 2211 56-57 0.07059977808577855 2211 58-59 0.06552341708258069 2211 60-61 0.1430514895611381 2211 62-63 0.2780247180016957 2211 64-65 0.21813505658638377 2211 66-67 0.3159358318081118 2211 68-69 0.36715424670930474 2211 70-71 0.2606322483779948 2211 72-73 0.34771789459777835 2211 74-75 0.16562593876433596 2211 76-77 0.12628485626248676 2211 78-79 0.09795233895616917 2211 80-81 0.1139642600591344 2211 82-83 -0.3016312135860879 2211 84-85 0.053620118132776895 2211 86-87 0.06365937330423321 2211 88-89 0.12423965018741256 2211 90-91 0.2005918602138017 2211 92-93 0.29589964220554776 2211 94-95 0.2061832360765905 2211 96-97 0.23261943173440613 2211 98-99 0.2140280985524008 2211 100 0.013112312187651298 2212 1 0.8485297839687327 2212 2 4.22018163911358 2212 3 2.4288436749925104 2212 4 1.2440007109968079 2212 5 0.8142342898997796 2212 6 0.7916725839259371 2212 7 0.7550212713348259 2212 8 0.7097779056957592 2212 9 0.606354649229992 2212 10-11 0.5677791200232107 2212 12-13 0.6332669994282796 2212 14-15 0.6834498493285395 2212 16-17 0.6654969383836544 2212 18-19 0.6852541793120324 2212 20-21 0.7117113503786783 2212 22-23 0.8050759579736635 2212 24-25 0.7826387716314898 2212 26-27 0.538208813901953 2212 28-29 0.5430528342677903 2212 30-31 0.5409208883492695 2212 32-33 0.6707714760078076 2212 34-35 0.6980458933890716 2212 36-37 0.7897717849366117 2212 38-39 0.8713255644445255 2212 40-41 0.6352008324317708 2212 42-43 0.5118815677454194 2212 44-45 0.8415159673995234 2212 46-47 0.6965672493320128 2212 48-49 0.5960862013192028 2212 50-51 0.7330716374693651 2212 52-53 0.7428323808039465 2212 54-55 0.4870813579675115 2212 56-57 0.6640915808674634 2212 58-59 0.5480446970363531 2212 60-61 0.6461886442801728 2212 62-63 0.8638688005427682 2212 64-65 0.6944932642373587 2212 66-67 0.7090962415531052 2212 68-69 0.6825850888658138 2212 70-71 0.7167430682068314 2212 72-73 0.8614261438902275 2212 74-75 0.6837248225587587 2212 76-77 0.653485791100259 2212 78-79 0.6877215988079044 2212 80-81 0.5765295087755646 2212 82-83 0.5694206483318496 2212 84-85 0.7020093419962876 2212 86-87 0.7604166882047565 2212 88-89 0.8753201439643696 2212 90-91 0.8317745494863509 2212 92-93 0.8753726679804643 2212 94-95 0.8509395308946814 2212 96-97 0.7601832805125355 2212 98-99 0.8040046741071656 2212 100 0.6782564344976727 2213 1 -0.4991056231318858 2213 2 -25.690576307096688 2213 3 -14.601160612021472 2213 4 -6.114619233988293 2213 5 -2.2259131684140883 2213 6 -1.4308360034655365 2213 7 -1.1621615831750347 2213 8 -1.0642777485121968 2213 9 -1.0082139285611404 2213 10-11 -1.0143117187027428 2213 12-13 -1.0905131259321337 2213 14-15 -1.3082280248684484 2213 16-17 -1.3204701734838977 2213 18-19 -1.316723352886008 2213 20-21 -1.394641207878216 2213 22-23 -1.4571060840348053 2213 24-25 -1.247294154239519 2213 26-27 -1.3441043669020587 2213 28-29 -1.293202742324901 2213 30-31 -1.4058662833226592 2213 32-33 -1.2835783731983454 2213 34-35 -1.1320581923169968 2213 36-37 -1.400941805111536 2213 38-39 -1.7342475002786983 2213 40-41 -1.4594178959393886 2213 42-43 -1.3507893916369937 2213 44-45 -1.2751251571451832 2213 46-47 -1.3527249924555704 2213 48-49 -1.5342609399053018 2213 50-51 -1.5255141503843817 2213 52-53 -1.5614261421754136 2213 54-55 -1.5219737563546474 2213 56-57 -1.4585760857461025 2213 58-59 -1.465008779454017 2213 60-61 -1.4606853581338015 2213 62-63 -1.4857621794942801 2213 64-65 -1.392641230402809 2213 66-67 -1.4553258454422675 2213 68-69 -1.6008876971480461 2213 70-71 -1.4414612448927286 2213 72-73 -1.4235865193717707 2213 74-75 -1.5299812208480645 2213 76-77 -1.2646187446888213 2213 78-79 -1.2942768178834392 2213 80-81 -1.4093328331742043 2213 82-83 -1.4132647864045111 2213 84-85 -1.5303293096576347 2213 86-87 -1.3283383992834743 2213 88-89 -1.4018768705205993 2213 90-91 -1.2997707640376284 2213 92-93 -1.1605018080363152 2213 94-95 -1.3956308579193148 2213 96-97 -1.557794355386342 2213 98-99 -1.3103125360623622 2213 100 -1.126115945586207 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 27.0 5 50.0 6 38.0 7 40.0 8 54.0 9 85.0 10 437.0 11 2198.0 12 6367.0 13 11947.0 14 15643.0 15 17416.0 16 18479.0 17 18890.0 18 19587.0 19 20378.0 20 21795.0 21 24382.0 22 26447.0 23 28056.0 24 28548.0 25 30220.0 26 32495.0 27 36453.0 28 42168.0 29 49792.0 30 60185.0 31 72119.0 32 90254.0 33 119274.0 34 160445.0 35 223933.0 36 334762.0 37 565026.0 38 597737.0 39 4970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.73436595447785 21.26033210082416 10.431343124237827 30.573958820460152 2 12.477802050910979 23.164223199021848 36.74607361137798 27.611901138689195 3 14.860593110641418 27.683967778733575 29.141378006008843 28.314061104616155 4 10.078927154753853 19.024141480198452 38.271183064217986 32.62574830082971 5 11.122353321842864 38.99999290096737 35.52666041702707 14.350993360162695 6 28.85931516359483 40.648743272195226 17.89207891966945 12.599862644540494 7 25.152019153946938 33.676697287556614 23.75193160882631 17.41935194967014 8 23.665755076937774 37.52470547491237 21.242640604754197 17.566898843395652 9 24.198021812015416 17.076724197126165 22.479459804808435 36.245794186049984 10-11 22.44745216838817 28.233409495695692 29.55006983364013 19.769068502276006 12-13 23.230797306026883 25.79510608069604 29.87388841141494 21.100208201862138 14-15 20.851361198268727 27.22879830716739 27.04723678477496 24.87260370978893 16-17 20.048884108280166 30.87740444043089 27.92112866315957 21.152582788129372 18-19 19.979648927202938 29.793407133024896 30.06398983267269 20.16295410709948 20-21 21.341205415909233 29.06603624336966 29.203400315808643 20.389358024912465 22-23 20.46809907857744 29.12195276659969 29.507815954285206 20.902132200537668 24-25 20.660380895988162 28.81126849478981 29.337539020226437 21.190811588995594 26-27 20.551204574300673 28.865031865051265 29.424433081153843 21.15933047949422 28-29 20.228356689238424 28.879151613335306 29.785336354508797 21.10715534291747 30-31 20.941974300972458 29.218497702945363 29.153368624798865 20.68615937128332 32-33 20.083557375330745 29.1552158730289 29.815535128678505 20.945691622961846 34-35 20.25001039875834 28.682163023805973 29.893175374173435 21.174651203262243 36-37 20.782820492063497 29.16138953043529 29.170641228476278 20.885148749024935 38-39 20.514990477647185 29.123551828273087 29.502543335565473 20.85891435851425 40-41 20.75257505045644 28.87736753552469 29.3163105803703 21.05374683364857 42-43 20.359565031589995 29.037202345145047 29.527630216042315 21.07560240722264 44-45 20.80841336356404 28.60351483260104 29.35272276881437 21.23534903502055 46-47 20.794435630025735 29.268235353374244 29.131103518585366 20.80622549801466 48-49 20.670956278459222 29.021427101568054 29.230547075687845 21.07706954428487 50-51 20.905696224729734 28.934255329786733 28.849333949224775 21.310714496258758 52-53 20.48734919708596 29.16268654934276 28.979066324177573 21.370897929393713 54-55 20.867255085231143 29.09412644842569 28.71493788396923 21.323680582373935 56-57 20.906160591414388 28.925247356952326 28.786661425933385 21.3819306256999 58-59 20.470456073430203 28.58582986918697 29.35249377498308 21.591220282399746 60-61 20.994810808471488 28.99800737134562 28.651097475394337 21.356084344788552 62-63 20.38851215849416 29.21215569588374 29.014802861700044 21.384529283922056 64-65 20.4542694398744 28.598382915263393 29.17845297363791 21.7688946712243 66-67 20.6443390034897 28.922386726528476 28.81868073441528 21.61459353556655 68-69 20.841924036387578 28.83252322171747 28.904538671083614 21.421014070811335 70-71 20.99007510694582 28.72146175799545 28.60218841307869 21.686274721980045 72-73 20.576807228634696 29.010693216783856 29.002074018022334 21.410425536559117 74-75 20.987899848099794 28.53962744711011 28.751638496161824 21.720834208628276 76-77 20.806561558261887 29.0100391125047 28.72472639342262 21.458672935810792 78-79 20.818813901047488 28.877527873579083 28.937968508190824 21.3656897171826 80-81 20.97081778731385 28.66852948413238 28.65875463582441 21.701898092729362 82-83 20.413780213327385 28.979836081658476 28.98410770305777 21.622276001956365 84-85 20.644505757606904 28.492855851743602 28.80472972330171 22.05790866734778 86-87 20.788823707278546 28.845688584363987 28.610934058134436 21.75455365022303 88-89 20.57539745457462 28.520273307728612 28.96869794571525 21.93563129198152 90-91 20.768231814073008 28.541093000429395 28.719679551485495 21.9709956340121 92-93 20.287891415111726 28.92776750227748 28.883691450477023 21.900649632133774 94-95 20.331991690881697 28.36714857986037 29.043695987140527 22.25716374211741 96-97 20.489826020312336 28.68114193882798 28.822430373712105 22.006601667147578 98-99 20.471907823835107 28.478568787996238 28.869158087106577 22.18036530106208 100 20.575665732070007 28.407717667163094 28.673681867679633 22.34293473308726 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 30.0 1 25.0 2 25.0 3 29.0 4 28.0 5 32.5 6 57.5 7 107.0 8 176.0 9 221.5 10 201.5 11 199.5 12 263.5 13 326.0 14 384.0 15 468.0 16 580.0 17 720.5 18 951.5 19 1247.0 20 1690.0 21 2480.0 22 3633.5 23 5469.0 24 7950.0 25 11319.5 26 16315.0 27 22831.5 28 31187.5 29 40959.5 30 51310.5 31 61797.5 32 71720.0 33 81195.5 34 90025.0 35 98080.5 36 104681.0 37 109609.5 38 113254.0 39 115622.0 40 115835.0 41 114020.0 42 111176.0 43 107214.5 44 102027.0 45 96533.0 46 91236.0 47 86482.5 48 81991.5 49 78670.5 50 81311.0 51 76933.0 52 64337.5 53 57068.5 54 52176.0 55 47838.0 56 43895.5 57 39544.5 58 34745.5 59 30279.5 60 25710.0 61 21022.0 62 17123.5 63 13691.0 64 10726.0 65 8361.0 66 6323.5 67 4676.5 68 3504.5 69 2569.5 70 1864.5 71 1375.5 72 1029.0 73 759.5 74 545.5 75 363.5 76 226.0 77 148.5 78 92.0 79 46.5 80 23.5 81 11.5 82 4.0 83 3.0 84 2.5 85 2.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7668852402755385 2 5.451271564217593 3 0.0 4 0.005259792726863067 5 0.16014390196044784 6 0.057223560588708824 7 0.03555023027447165 8 0.0427498047162062 9 0.040660809023267686 10-11 0.042208904045713196 12-13 0.04114575445198555 14-15 0.04407407877155116 16-17 0.03381561777944234 18-19 0.028238745349186824 20-21 0.022755131655223197 22-23 0.02309086310587403 24-25 0.01990141432469111 26-27 0.01298161609183225 28-29 0.019435120643231617 30-31 0.02430322667766871 32-33 0.0015294432751871332 34-35 0.00393551867151811 36-37 0.004662936814594917 38-39 0.006677325518499922 40-41 0.006863842991083719 42-43 0.007367440167059969 44-45 0.008076206562878397 46-47 0.016656010301733047 48-49 0.025123903557037416 50-51 0.021934454775854494 52-53 0.027082337019167284 54-55 0.024209967941376812 56-57 0.015947243905914617 58-59 0.014921397806703736 60-61 0.01249667066311438 62-63 0.018241408818695317 64-65 0.019416468895973236 66-67 0.020181190533566803 68-69 0.027362113228042977 70-71 0.027436720217076497 72-73 0.024265923183151954 74-75 0.02480682385364496 76-77 0.018539836774829392 78-79 0.014846790817670218 80-81 0.01363442724587554 82-83 0.008691714222404926 84-85 0.01132161058583646 86-87 0.009493739354515252 88-89 0.008169465299170295 90-91 0.008244072288203815 92-93 0.0044764193420111205 94-95 0.0016600055059957906 96-97 0.0010258460992108819 98-99 7.833733848519461E-4 100 0.10855316904376969 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2680714.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.05768272118644 #Duplication Level Percentage of deduplicated Percentage of total 1 91.59573098066146 80.65707817310233 2 6.757123322931123 11.900332433571958 3 1.0505024988349758 2.775144472206715 4 0.2657981210298106 0.9362226643812229 5 0.11026733882473094 0.4854943168368861 6 0.06017542920580543 0.31793453115696185 7 0.03544811109452182 0.218503496387874 8 0.02730936599504996 0.19238395888870938 9 0.015956375594840932 0.12645733135595266 >10 0.07633646824335663 1.0950206926541055 >50 0.002753416046825107 0.17029872008922894 >100 0.0020042208341497277 0.3961101539892739 >500 2.5472173005444275E-4 0.16534799663286062 >1k 3.3962897333083327E-4 0.5636710587458728 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4220 0.1574207468607244 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3139 0.11709566928810758 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 7.460698903351868E-5 0.0 8 0.0 0.0 0.0 7.460698903351868E-5 0.0 9 0.0 0.0 0.0 3.3573145065083406E-4 0.0 10-11 0.0 0.0 0.0 3.730349451675934E-4 0.0 12-13 0.0 0.0 0.0 3.730349451675934E-4 0.0 14-15 0.0 0.0 0.0 4.476419342011121E-4 0.0 16-17 0.0 0.0 0.0 4.662936814594918E-4 0.0 18-19 0.0 0.0 0.0 4.8494542871787145E-4 0.0 20-21 0.0 0.0 0.0 5.222489232346308E-4 0.0 22-23 0.0 0.0 0.0 5.968559122681495E-4 0.0 24-25 0.0 0.0 0.0 6.341594067849087E-4 0.0 26-27 0.0 0.0 0.0 7.460698903351868E-4 0.0 28-29 0.0 0.0 0.0 0.001231015319053058 0.0 30-31 0.0 0.0 0.0 0.004122036144101907 0.0 32-33 0.0 0.0 0.0 0.012142287465205166 0.0 34-35 0.0 0.0 0.0 0.02488143084267848 0.0 36-37 1.865174725837967E-5 1.865174725837967E-5 0.0 0.04342126761750788 0.0 38-39 3.730349451675934E-5 3.730349451675934E-5 0.0 0.07158540597766118 0.0 40-41 3.730349451675934E-5 5.5955241775139015E-5 0.0 0.10168932605268596 0.0 42-43 3.730349451675934E-5 7.460698903351868E-5 0.0 0.13207302233658644 0.0 44-45 3.730349451675934E-5 7.460698903351868E-5 0.0 0.17133495031547566 0.0 46-47 3.730349451675934E-5 1.1191048355027803E-4 0.0 0.22061286657211476 0.0 48-49 3.730349451675934E-5 1.4921397806703736E-4 0.0 0.27207303725798426 0.0 50-51 3.730349451675934E-5 1.4921397806703736E-4 0.0 0.32627501479083554 0.0 52-53 3.730349451675934E-5 1.4921397806703736E-4 0.0 0.3809805894996632 0.0 54-55 3.730349451675934E-5 1.6786572532541703E-4 0.0 0.44227023099069873 0.0 56-57 3.730349451675934E-5 1.865174725837967E-4 0.0 0.5183507080576294 0.0 58-59 3.730349451675934E-5 1.865174725837967E-4 0.0 0.6021530084895292 0.0 60-61 3.730349451675934E-5 2.984279561340747E-4 0.0 0.6923528582310534 0.0 62-63 3.730349451675934E-5 2.984279561340747E-4 0.0 0.7835039470827548 0.0 64-65 3.730349451675934E-5 2.984279561340747E-4 0.0 0.8821903418268416 0.0 66-67 3.730349451675934E-5 3.5438319790921376E-4 0.0 1.0022329871817732 0.0 68-69 3.730349451675934E-5 3.730349451675934E-4 0.0 1.1377379310139015 0.0 70-71 3.730349451675934E-5 3.730349451675934E-4 0.0 1.2881456209054751 0.0 72-73 3.730349451675934E-5 3.730349451675934E-4 0.0 1.4405490477536955 0.0 74-75 3.730349451675934E-5 4.1033843968435273E-4 0.0 1.6034161048138666 0.0 76-77 3.730349451675934E-5 4.1033843968435273E-4 0.0 1.7886652585840936 0.0 78-79 3.730349451675934E-5 4.1033843968435273E-4 0.0 2.0008848388899376 0.0 80-81 3.730349451675934E-5 4.1033843968435273E-4 0.0 2.2280444687497436 0.0 82-83 3.730349451675934E-5 4.1033843968435273E-4 0.0 2.4745646122637477 0.0 84-85 3.730349451675934E-5 4.1033843968435273E-4 0.0 2.731305913275344 0.0 86-87 3.730349451675934E-5 4.1033843968435273E-4 0.0 3.0099070620737614 0.0 88 3.730349451675934E-5 4.1033843968435273E-4 0.0 3.24152445952832 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 165 6.8380075E-5 19.948923 3 GTATCAA 8340 0.0 18.97419 1 GAGTACT 3520 0.0 17.073545 12-13 CAACGCA 9140 0.0 17.029556 5 TCAACGC 9195 0.0 16.978836 4 AACGCAG 9160 0.0 16.91827 6 ATCAACG 9300 0.0 16.887634 3 TATCAAC 9595 0.0 16.42853 2 CGCAGAG 9920 0.0 15.572156 8 ACGCAGA 9995 0.0 15.549261 7 ATACCGT 245 8.087841E-5 15.334209 6 CCTAGAC 965 0.0 15.10566 3 GTATAGG 725 0.0 14.413964 1 GTATTAC 1040 0.0 14.354567 1 AGTACTT 4135 0.0 14.307096 12-13 GTACATG 10030 0.0 14.239119 1 TACATGG 10180 0.0 14.116742 2 GTACTAT 955 0.0 14.068978 1 ACATGGG 9985 0.0 14.033743 3 GTACTAG 750 0.0 13.933499 1 >>END_MODULE