FastQCFastQC Report
Wed 25 May 2016
SRR1295098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933548
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT1150.001315261924.7027364
CGTACTA1300.00525874819.456565
GTATAGA8750.019.3989491
GTCCTAG6000.018.8600861
TACATCG2008.128014E-418.8556272
GTACTGG6850.018.3553161
TATACGT2302.0447186E-417.2919144
GTATTAG9350.016.8093471
GTGTAGG5656.002665E-1116.6903441
GTACTAG3405.9082067E-616.6412541
CTAGTAC3801.4766738E-716.4468213
GTATAGG5104.7039066E-916.0249121
TATACTG12850.015.3532715
GTCTAGG5401.0017175E-815.1346391
GGTATAC3802.6623075E-614.9516543
GTATAGC4252.9753337E-614.7922271
GTACATG72750.014.7769761
CCCTAGG5201.0342592E-714.507761
GTATAAA13100.014.3970141
TACATGG73000.014.2923952
Produced by FastQC (version 0.11.5)