##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295098_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1933548
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.58531880253296	27.0	22.0	33.0	6.0	33.0
2	21.618671478546176	27.0	2.0	33.0	2.0	33.0
3	23.986793707733142	27.0	15.0	33.0	6.0	33.0
4	23.760495731163644	33.0	2.0	37.0	2.0	37.0
5	25.764879382358234	33.0	15.0	37.0	2.0	37.0
6	26.694000873006516	33.0	15.0	37.0	2.0	37.0
7	26.5577854803708	33.0	15.0	37.0	6.0	37.0
8	27.191323928860314	33.0	15.0	37.0	2.0	37.0
9	28.220734628775702	33.0	22.0	37.0	6.0	37.0
10-11	27.767602614468323	33.0	15.0	37.0	4.0	37.0
12-13	26.555613049171782	33.0	15.0	37.0	2.0	37.0
14-15	28.23055026303976	33.0	15.0	37.0	4.0	40.0
16-17	28.65321057455	33.0	22.0	37.0	4.0	40.0
18-19	28.91142992053986	33.0	18.5	38.5	6.0	40.0
20-21	28.38378592101153	33.0	15.0	37.0	4.0	40.0
22-23	29.168175809444605	33.0	22.0	37.0	6.0	40.0
24-25	29.339255865383222	33.0	24.5	37.0	6.0	40.0
26-27	27.84892694673212	33.0	15.0	37.0	4.0	40.0
28-29	28.586481949245634	33.0	18.5	37.0	6.0	40.0
30-31	29.34545871113621	33.0	22.0	37.0	6.0	40.0
32-33	28.037466874367745	33.0	18.5	37.0	4.0	40.0
34-35	27.70029888060705	33.0	15.0	37.0	6.0	40.0
36-37	27.975288950675132	33.0	18.5	37.0	6.0	40.0
38-39	28.10161035567775	33.0	18.5	37.0	6.0	40.0
40-41	27.194858105410365	33.0	15.0	37.0	4.0	40.0
42-43	27.799415892442287	33.0	15.0	37.0	6.0	40.0
44-45	27.707838646881278	33.0	18.5	37.0	6.0	40.0
46-47	27.456565598578365	33.0	15.0	37.0	6.0	40.0
48-49	27.466448725348428	33.0	15.0	37.0	6.0	40.0
50-51	27.36101663884217	33.0	18.5	37.0	6.0	40.0
52-53	27.59638472900595	33.0	18.5	37.0	6.0	40.0
54-55	27.54606841929965	33.0	22.0	37.0	6.0	40.0
56-57	27.555397900646895	33.0	22.0	37.0	6.0	40.0
58-59	27.4843952671462	33.0	22.0	37.0	6.0	40.0
60-61	27.438280559882664	33.0	22.0	37.0	6.0	40.0
62-63	27.496096036922797	33.0	22.0	37.0	6.0	40.0
64-65	27.047671172373274	33.0	22.0	37.0	6.0	40.0
66-67	27.224025470275368	33.0	22.0	37.0	6.0	40.0
68-69	26.77451865689396	33.0	18.5	37.0	6.0	38.5
70-71	26.95039016357494	33.0	22.0	37.0	6.0	37.0
72-73	27.058404808155785	27.0	22.0	37.0	6.0	37.0
74-75	27.184453915806593	27.0	22.0	37.0	6.0	37.0
76-77	26.954302401595406	27.0	22.0	37.0	6.0	37.0
78-79	26.51551138115009	27.0	22.0	35.0	6.0	37.0
80-81	26.325685734204686	27.0	22.0	33.0	6.0	37.0
82-83	26.45867570911092	27.0	22.0	33.0	6.0	37.0
84-85	26.41308516778482	27.0	22.0	33.0	6.0	37.0
86-87	26.256795021380384	27.0	22.0	33.0	6.0	37.0
88-89	26.241441898520232	27.0	22.0	33.0	6.0	37.0
90-91	26.3864395918798	27.0	22.0	33.0	6.0	37.0
92-93	26.26409248697214	27.0	22.0	33.0	6.0	37.0
94-95	26.133712222298076	27.0	22.0	33.0	6.0	37.0
96-97	26.20217160370469	27.0	22.0	33.0	6.0	37.0
98-99	26.30357844749652	27.0	22.0	33.0	10.5	37.0
100	25.919993193859163	27.0	22.0	33.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.137481054836144
1101	2	11.030885029758611
1101	3	8.348008000082295
1101	4	12.59867726145109
1101	5	10.626111295801511
1101	6	9.68784248595276
1101	7	9.132312952785096
1101	8	8.69161235483178
1101	9	7.104135227874153
1101	10-11	7.453155064031893
1101	12-13	8.895223247700425
1101	14-15	7.9321472757013005
1101	16-17	8.106042915040327
1101	18-19	7.847113597195548
1101	20-21	7.094657366174065
1101	22-23	7.1270538821048355
1101	24-25	7.09825679202045
1101	26-27	8.381593502198797
1101	28-29	8.095162782480106
1101	30-31	5.5126404746999
1101	32-33	6.942608318037774
1101	34-35	8.566126370341163
1101	36-37	8.051492913375697
1101	38-39	7.9403886893035285
1101	40-41	8.757189353301776
1101	42-43	8.324797232280837
1101	44-45	8.315972447318096
1101	46-47	7.37440326089909
1101	48-49	5.562075806059916
1101	50-51	6.922100691816482
1101	52-53	5.9748460510338965
1101	54-55	4.175737879268443
1101	56-57	6.156400962432677
1101	58-59	6.176114855100735
1101	60-61	6.724272200147336
1101	62-63	6.234127075368306
1101	64-65	6.614225998161974
1101	66-67	5.767663874731156
1101	68-69	6.893068287191511
1101	70-71	6.383300239592966
1101	72-73	5.515934018166401
1101	74-75	4.896929659067229
1101	76-77	4.969588316208242
1101	78-79	5.101808858011768
1101	80-81	4.969225097618651
1101	82-83	4.393916275017364
1101	84-85	4.361152648071396
1101	86-87	4.13972328393757
1101	88-89	4.070266718948151
1101	90-91	3.9432179360975574
1101	92-93	4.0141306938984656
1101	94-95	3.7908961578070404
1101	96-97	4.013551538133971
1101	98-99	3.622862534328167
1101	100	3.8601265597708014
1102	1	6.8275065515547695
1102	2	10.476844284598847
1102	3	8.156033738800314
1102	4	12.477231627506907
1102	5	10.539090509144803
1102	6	9.480770103339825
1102	7	6.740946256265229
1102	8	7.927480371438488
1102	9	6.563531151771301
1102	10-11	7.3719489264215134
1102	12-13	8.914994207426371
1102	14-15	6.237485947154397
1102	16-17	7.3601139163227245
1102	18-19	7.338618296595609
1102	20-21	7.109373086315653
1102	22-23	6.650269241974787
1102	24-25	6.874940014321687
1102	26-27	8.446582302288455
1102	28-29	7.6514613001346525
1102	30-31	4.451934797285087
1102	32-33	4.7372154570694
1102	34-35	5.070897273038565
1102	36-37	5.199682424437739
1102	38-39	4.627882242598439
1102	40-41	6.251609885016091
1102	42-43	6.841713262459351
1102	44-45	6.444934350514373
1102	46-47	5.595967774564613
1102	48-49	3.9088473271451036
1102	50-51	5.215109760842843
1102	52-53	5.976350829778021
1102	54-55	5.417917034194669
1102	56-57	6.23197918848788
1102	58-59	5.921375380090641
1102	60-61	6.044930257487309
1102	62-63	4.467369516358637
1102	64-65	6.148808913699227
1102	66-67	3.9466719834464072
1102	68-69	6.128273904850328
1102	70-71	5.444819782531496
1102	72-73	5.156512883933107
1102	74-75	4.540332863525972
1102	76-77	3.561720348199767
1102	78-79	4.511735556495406
1102	80-81	4.747838426231013
1102	82-83	4.073364867255332
1102	84-85	3.1303531363845103
1102	86-87	3.4379866655616844
1102	88-89	3.5195550460976044
1102	90-91	3.500712317546121
1102	92-93	3.434049537319897
1102	94-95	3.2956631242950856
1102	96-97	3.4569812050773514
1102	98-99	3.1846544195629285
1102	100	3.4011669042430484
1103	1	6.5259627268535745
1103	2	10.806276555515183
1103	3	7.8796393164895555
1103	4	11.84974158766627
1103	5	10.190982939423687
1103	6	8.975043011706358
1103	7	8.019830718416625
1103	8	7.915030172235301
1103	9	6.985343900775284
1103	10-11	7.357133189369723
1103	12-13	8.6881515779443
1103	14-15	8.492341524843642
1103	16-17	7.9930820438127625
1103	18-19	7.299151165121504
1103	20-21	8.301106154044735
1103	22-23	6.8395122698632385
1103	24-25	5.37855057209061
1103	26-27	6.097727720615147
1103	28-29	6.905818869855768
1103	30-31	6.051909896886684
1103	32-33	7.948993345515614
1103	34-35	7.2400954802098845
1103	36-37	4.208522065872003
1103	38-39	6.465905150964971
1103	40-41	6.82133100055394
1103	42-43	7.518630593136642
1103	44-45	5.783704270833098
1103	46-47	6.454284507632341
1103	48-49	5.5994598769459
1103	50-51	6.366255179169535
1103	52-53	6.157625730176424
1103	54-55	5.069684962481521
1103	56-57	5.986336758208992
1103	58-59	5.403073587261957
1103	60-61	4.974337628005237
1103	62-63	2.7427679227331403
1103	64-65	4.960810905731101
1103	66-67	4.260915011334852
1103	68-69	5.3157739048503245
1103	70-71	3.741383527551413
1103	72-73	4.36953579229964
1103	74-75	3.4733507918128232
1103	76-77	3.7080101888372177
1103	78-79	3.0174626481288733
1103	80-81	4.201772689179219
1103	82-83	3.5459744290083215
1103	84-85	3.58988998897415
1103	86-87	2.899391048031802
1103	88-89	2.9865620182091597
1103	90-91	2.6847262617692316
1103	92-93	2.555065473574878
1103	94-95	2.7065196980002604
1103	96-97	2.984122639340299
1103	98-99	2.690381511196396
1103	100	3.063766505836675
1104	1	6.336218308301369
1104	2	9.749083629107606
1104	3	7.327710490911368
1104	4	11.906481546071642
1104	5	9.382345226125931
1104	6	8.023361248194366
1104	7	4.114551082244333
1104	8	6.53295325975942
1104	9	6.562557687139261
1104	10-11	6.874362197864048
1104	12-13	7.406118170692803
1104	14-15	7.112923223207414
1104	16-17	7.205706796374841
1104	18-19	6.770781687058911
1104	20-21	7.273180623462668
1104	22-23	6.320709524868374
1104	24-25	5.8632159044717795
1104	26-27	8.085440266657393
1104	28-29	7.1386806616821765
1104	30-31	6.295320217866909
1104	32-33	7.343734193444657
1104	34-35	7.742281766511223
1104	36-37	6.596127180006661
1104	38-39	7.224266078324714
1104	40-41	6.874145309356464
1104	42-43	7.1024728655564004
1104	44-45	6.535283676480947
1104	46-47	6.444398141148593
1104	48-49	5.306263480143176
1104	50-51	5.570132312147059
1104	52-53	5.976560275015938
1104	54-55	4.691673079197049
1104	56-57	5.7660690241139285
1104	58-59	3.7048150490876104
1104	60-61	4.181388136662431
1104	62-63	3.690597265738475
1104	64-65	2.6018158169040184
1104	66-67	3.291099274283063
1104	68-69	4.1863014800839835
1104	70-71	2.7438346663922744
1104	72-73	2.4772468349216084
1104	74-75	3.210541055913655
1104	76-77	3.8609327438999834
1104	78-79	4.19253994898833
1104	80-81	2.9352043086134323
1104	82-83	2.8696277089702313
1104	84-85	2.979664538574493
1104	86-87	0.7410267066551732
1104	88-89	0.33086061000549805
1104	90-91	2.188926692749707
1104	92-93	2.3969108593274626
1104	94-95	2.4619788676111263
1104	96-97	2.8556065778002946
1104	98-99	2.5397331925214104
1104	100	0.9520402661413669
1105	1	5.630651820991197
1105	2	-2.781211528267068
1105	3	0.4701401798532565
1105	4	8.089580201607799
1105	5	8.261861389505977
1105	6	7.8257705879826425
1105	7	5.147397077010339
1105	8	5.802316804487333
1105	9	4.7886150149641225
1105	10-11	4.807502829444989
1105	12-13	4.8885779574526005
1105	14-15	5.849053003483878
1105	16-17	5.908394115066251
1105	18-19	5.895517455665374
1105	20-21	6.747642057084196
1105	22-23	2.016064806893443
1105	24-25	3.8753560202253325
1105	26-27	6.621836952111536
1105	28-29	5.7495007325729475
1105	30-31	5.082036006443943
1105	32-33	7.017920108990932
1105	34-35	5.877713618142227
1105	36-37	6.043434797688654
1105	38-39	3.8954233772821745
1105	40-41	5.2926259374354565
1105	42-43	5.682094276651892
1105	44-45	6.102862363818566
1105	46-47	5.292284526618349
1105	48-49	5.0199471028603995
1105	50-51	5.824717355045916
1105	52-53	5.6137464129832
1105	54-55	5.2770031377363615
1105	56-57	5.157069303323244
1105	58-59	2.0535886576550766
1105	60-61	4.3058831227256285
1105	62-63	-0.7903150388741551
1105	64-65	-0.26361456769498304
1105	66-67	0.9363112130841671
1105	68-69	3.00256736307162
1105	70-71	3.008927160394009
1105	72-73	2.829062121395097
1105	74-75	2.719281821893656
1105	76-77	1.7998033185506195
1105	78-79	3.208522836769845
1105	80-81	1.7443276386814368
1105	82-83	1.1301757164095072
1105	84-85	1.4406045748243805
1105	86-87	-0.15403838697459804
1105	88-89	1.531705809734806
1105	90-91	1.3315859012149573
1105	92-93	1.751482651614662
1105	94-95	1.0399114882508478
1105	96-97	1.9604806260041272
1105	98-99	1.8233105737273085
1105	100	1.9397705783352421
1106	1	6.0635489120189945
1106	2	10.099792925034635
1106	3	6.652281446929734
1106	4	11.577679810060925
1106	5	9.943903905091414
1106	6	9.299923051606076
1106	7	6.568331475644754
1106	8	7.447634923767975
1106	9	6.773416769951076
1106	10-11	5.653004695928754
1106	12-13	7.888894761906425
1106	14-15	7.756921612796322
1106	16-17	7.6566793230103585
1106	18-19	7.1011661649344155
1106	20-21	8.116814649770195
1106	22-23	6.1544256883593
1106	24-25	5.065475081172423
1106	26-27	7.4330379872106604
1106	28-29	6.033558590656202
1106	30-31	5.253996385337231
1106	32-33	7.165019133613836
1106	34-35	6.548791122750938
1106	36-37	5.958501510426952
1106	38-39	6.933283105779427
1106	40-41	6.618856974266599
1106	42-43	6.149906342831883
1106	44-45	4.433886660404816
1106	46-47	5.729250141882293
1106	48-49	5.542081136839592
1106	50-51	6.363275131983098
1106	52-53	5.529899795063688
1106	54-55	5.265171122143418
1106	56-57	4.9664250555387035
1106	58-59	4.954656184209803
1106	60-61	4.830643927769106
1106	62-63	4.847362950924875
1106	64-65	5.070438386908716
1106	66-67	3.1692950231708537
1106	68-69	3.3542677298469314
1106	70-71	2.993949757933887
1106	72-73	2.9673979275451146
1106	74-75	2.973595303139735
1106	76-77	3.6499973686400047
1106	78-79	4.267107174324632
1106	80-81	3.676485922257779
1106	82-83	2.1440890762030307
1106	84-85	2.6763613304988105
1106	86-87	3.29495153962943
1106	88-89	3.3104012386876
1106	90-91	3.0995388354980413
1106	92-93	2.8276679701629206
1106	94-95	1.6508368436539307
1106	96-97	1.5801376155206022
1106	98-99	1.9473731319600027
1106	100	2.9249504157728055
1107	1	6.170915851452751
1107	2	10.253416055958187
1107	3	5.9644867765749225
1107	4	11.14554179446661
1107	5	9.31852958789666
1107	6	8.00969896441854
1107	7	3.7185689502342747
1107	8	6.166311451279601
1107	9	5.542814118961477
1107	10-11	4.3781428851088044
1107	12-13	6.152552703013118
1107	14-15	6.180031823579352
1107	16-17	7.0851583275830805
1107	18-19	6.248539964554784
1107	20-21	5.746077630200542
1107	22-23	5.956080481908561
1107	24-25	4.791828798955315
1107	26-27	7.281941336680852
1107	28-29	6.52644267725125
1107	30-31	5.314813335139611
1107	32-33	5.218366694122178
1107	34-35	6.723665040502642
1107	36-37	6.684922679366515
1107	38-39	6.589730861002483
1107	40-41	7.694299317972721
1107	42-43	6.240317212187929
1107	44-45	4.146549201166248
1107	46-47	3.557819599557856
1107	48-49	4.231881897629883
1107	50-51	4.898521482948972
1107	52-53	4.022169721126939
1107	54-55	5.414241789655247
1107	56-57	5.526023232717101
1107	58-59	3.2243338656136302
1107	60-61	3.160127097664983
1107	62-63	0.915099214209139
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2104	5	10.104275400528746
2104	6	9.03888351252952
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2205	5	9.862769870324161
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2205	9	6.855854242699969
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2205	78-79	2.2552295167722356
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2205	98-99	2.7738995887632782
2205	100	2.884363601483571
2206	1	5.9232372872681225
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2206	3	7.0684804453532095
2206	4	11.541606545686083
2206	5	9.754851911793157
2206	6	8.431531549951611
2206	7	7.857287902920394
2206	8	7.251199594594908
2206	9	5.479570281178141
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2206	12-13	6.778398669519149
2206	14-15	7.584882347331035
2206	16-17	7.322129817895444
2206	18-19	6.681749389194312
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2206	22-23	6.412560012683034
2206	24-25	5.635836716869022
2206	26-27	7.314968614811782
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2206	30-31	6.211496293819792
2206	32-33	7.88248607884908
2206	34-35	7.737225767300853
2206	36-37	6.6752364979914915
2206	38-39	7.221918676558527
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2206	52-53	6.16019801826781
2206	54-55	6.137709272793
2206	56-57	3.704377542594745
2206	58-59	4.794692008134621
2206	60-61	5.453912157254596
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2206	64-65	5.474945881277666
2206	66-67	4.965385689192331
2206	68-69	5.187886484448562
2206	70-71	4.51466082053739
2206	72-73	4.192994287890599
2206	74-75	1.7298518229995032
2206	76-77	2.474336558200374
2206	78-79	1.213088189123308
2206	80-81	2.1908830696289776
2206	82-83	3.1350643054026968
2206	84-85	3.633551170632998
2206	86-87	-1.2490384072710654
2206	88-89	-1.1307772319505744
2206	90-91	0.45333476058416977
2206	92-93	2.026872580605314
2206	94-95	2.777214847759243
2206	96-97	1.6586898193695667
2206	98-99	1.455861828768679
2206	100	1.8768260626071402
2207	1	6.284116284136779
2207	2	10.53640667028698
2207	3	7.617887338328416
2207	4	11.707680586617936
2207	5	10.287517701912943
2207	6	9.153223461176491
2207	7	5.785227109884712
2207	8	6.8192222848014055
2207	9	6.237292738699907
2207	10-11	7.01042662447367
2207	12-13	8.812908748869685
2207	14-15	8.379802309605882
2207	16-17	7.434033453176532
2207	18-19	4.311296755737299
2207	20-21	7.476779711950908
2207	22-23	6.343105854235734
2207	24-25	4.385418683568467
2207	26-27	3.9879649636780776
2207	28-29	5.128095016916685
2207	30-31	5.552670779571429
2207	32-33	6.912236330088444
2207	34-35	6.304788516031415
2207	36-37	6.725494914764731
2207	38-39	6.970065610276034
2207	40-41	4.2943119998404455
2207	42-43	5.763218178538985
2207	44-45	4.173476418499458
2207	46-47	5.584998276062166
2207	48-49	2.4265476898769265
2207	50-51	5.45995654035076
2207	52-53	3.0865532220170273
2207	54-55	3.261627703367477
2207	56-57	5.466275420369538
2207	58-59	3.9145862952864405
2207	60-61	3.769845220162871
2207	62-63	4.5224669558214075
2207	64-65	3.512770061533981
2207	66-67	3.7370899901394026
2207	68-69	3.9250698021309844
2207	70-71	3.8357192922375987
2207	72-73	3.8812115422682467
2207	74-75	3.6345420577775513
2207	76-77	4.2166759594221155
2207	78-79	4.3297132791255315
2207	80-81	4.397212953531049
2207	82-83	3.9867377926074283
2207	84-85	3.3972395669632007
2207	86-87	3.628299808299797
2207	88-89	3.5055254388972834
2207	90-91	1.3194199570589227
2207	92-93	-0.4297856788639862
2207	94-95	1.621227346760218
2207	96-97	-0.7095926655773397
2207	98-99	1.0178363814296105
2207	100	2.1845414685317053
2208	1	1.0124649439086006
2208	2	8.389678585820224
2208	3	6.3938800244277765
2208	4	11.548576828407388
2208	5	9.713707332702608
2208	6	8.898544576290409
2208	7	7.228815561087938
2208	8	7.541723315183663
2208	9	6.36054656585717
2208	10-11	6.314754129697235
2208	12-13	6.645061056852224
2208	14-15	7.677681146323923
2208	16-17	7.032909488799888
2208	18-19	6.17109574259246
2208	20-21	6.342813329686848
2208	22-23	5.613212815255881
2208	24-25	5.874593404141599
2208	26-27	7.600789093275601
2208	28-29	6.796357397617765
2208	30-31	5.767297501669091
2208	32-33	7.95566019729748
2208	34-35	6.166258376296309
2208	36-37	6.564946056128697
2208	38-39	6.9900536991809865
2208	40-41	7.346086341968871
2208	42-43	6.633321990472716
2208	44-45	5.601807251139725
2208	46-47	4.452199797955483
2208	48-49	4.41859571592164
2208	50-51	5.720609725743081
2208	52-53	5.425016912490374
2208	54-55	6.1233537954768416
2208	56-57	6.150537666379353
2208	58-59	5.55488632184138
2208	60-61	4.93242871886747
2208	62-63	3.2273911303612337
2208	64-65	3.2583224045103023
2208	66-67	2.8373073360740335
2208	68-69	5.2778605089528625
2208	70-71	5.161510946910905
2208	72-73	4.3787978029103485
2208	74-75	2.181912544079644
2208	76-77	3.376833718036856
2208	78-79	4.031336190887597
2208	80-81	3.3608972938260457
2208	82-83	1.2403177112017794
2208	84-85	1.9758631732344583
2208	86-87	0.9906662441337097
2208	88-89	0.6839326250889037
2208	90-91	1.7142589877323822
2208	92-93	0.8381342003573451
2208	94-95	-0.9287903771709303
2208	96-97	1.4791546402288525
2208	98-99	0.21731520172994223
2208	100	1.8683160484338437
2209	1	5.297633245445713
2209	2	9.524597096251547
2209	3	5.603239737466918
2209	4	-3.1900581517241804
2209	5	0.6452705853171423
2209	6	4.266251126165919
2209	7	2.1539916467101143
2209	8	5.057654805812394
2209	9	5.108452869328584
2209	10-11	5.13794157923326
2209	12-13	7.171316543646832
2209	14-15	6.6528853765731135
2209	16-17	6.259639208394141
2209	18-19	5.345935641118967
2209	20-21	5.876234822897057
2209	22-23	5.550083902637066
2209	24-25	5.177188708191764
2209	26-27	7.051890123609688
2209	28-29	6.165038566882945
2209	30-31	4.222613360110671
2209	32-33	6.245946155556101
2209	34-35	5.686352525345217
2209	36-37	6.130581210867838
2209	38-39	6.713362684553541
2209	40-41	7.214084048111388
2209	42-43	6.658289495020185
2209	44-45	6.4322246264494325
2209	46-47	6.632611309920929
2209	48-49	5.177825656863604
2209	50-51	4.638169841396611
2209	52-53	4.8502454263500745
2209	54-55	5.443808943823473
2209	56-57	5.189113013340812
2209	58-59	3.1937788926768107
2209	60-61	4.210621278217527
2209	62-63	4.189946567117751
2209	64-65	5.050827298147627
2209	66-67	4.494641288089298
2209	68-69	3.8649131014559543
2209	70-71	3.6000350319835803
2209	72-73	0.9973885801484847
2209	74-75	2.365880500400518
2209	76-77	3.440330461424562
2209	78-79	3.4988871050263484
2209	80-81	3.7131795136784156
2209	82-83	3.1537447580705518
2209	84-85	3.098932787074773
2209	86-87	1.6716356212741843
2209	88-89	2.1662168219885665
2209	90-91	2.2871531407802017
2209	92-93	2.4260540851841412
2209	94-95	0.7689915435388421
2209	96-97	0.18001385429480976
2209	98-99	0.5700791868114798
2209	100	0.4289158228746395
2210	1	1.5699576111129083
2210	2	-16.941482408628243
2210	3	-7.416096303176378
2210	4	-8.116490828997968
2210	5	-1.587551110134914
2210	6	2.432421121795109
2210	7	-8.548457056559831
2210	8	-8.613643718715922
2210	9	-3.6825894295373693
2210	10-11	-1.7042039829267388
2210	12-13	2.8721137053350887
2210	14-15	-5.410313088223184
2210	16-17	-1.7421504961060883
2210	18-19	-0.7795988901677653
2210	20-21	1.8423853912793895
2210	22-23	1.995982887527969
2210	24-25	1.329039875370313
2210	26-27	3.5827543080104896
2210	28-29	2.8006137426257247
2210	30-31	2.1070742246824565
2210	32-33	4.067735746612552
2210	34-35	1.905910355018797
2210	36-37	3.514347167846992
2210	38-39	2.5856937666080064
2210	40-41	3.6471392959399545
2210	42-43	1.6937026782055185
2210	44-45	2.6955188996721944
2210	46-47	2.1771923617290874
2210	48-49	2.4575686009990534
2210	50-51	1.585575501454727
2210	52-53	1.7942324646250363
2210	54-55	2.1546551615660405
2210	56-57	2.1291002560724017
2210	58-59	1.0175908768903383
2210	60-61	2.085803118601749
2210	62-63	1.1673337738696183
2210	64-65	2.0243901926991263
2210	66-67	2.1848008719001726
2210	68-69	2.8759511487585314
2210	70-71	1.9822022051831567
2210	72-73	0.6838113194895534
2210	74-75	-0.025097096838365474
2210	76-77	-0.8305129795237285
2210	78-79	-0.1623229481807158
2210	80-81	0.3078943034532351
2210	82-83	0.3617302589245064
2210	84-85	-1.2809084986351174
2210	86-87	-1.258451653730873
2210	88-89	-1.6614466106925114
2210	90-91	-2.0028234311460835
2210	92-93	-1.4213742185097473
2210	94-95	-0.6640996388953191
2210	96-97	-0.24569900287502477
2210	98-99	-0.6774048912576909
2210	100	-0.530331176005685
2211	1	1.0890630107687116
2211	2	-16.941482408628243
2211	3	-7.821602523118628
2211	4	-18.67565681870823
2211	5	-20.898409490855197
2211	6	-13.197857910005924
2211	7	-11.19999173343056
2211	8	-18.63598239578001
2211	9	-15.561752907926053
2211	10-11	-9.859498469770205
2211	12-13	-22.004951011697134
2211	14-15	-17.441846975147687
2211	16-17	-15.518678786595647
2211	18-19	-18.712645985897062
2211	20-21	-8.451969352347689
2211	22-23	-4.270562850554949
2211	24-25	-2.230468178740608
2211	26-27	-17.134109805911546
2211	28-29	-4.18999616463616
2211	30-31	-4.993975863410302
2211	32-33	-1.0857287963956956
2211	34-35	-5.871032215104652
2211	36-37	-6.858606733878197
2211	38-39	-2.7514281645393375
2211	40-41	-5.649345256681272
2211	42-43	-1.7725343913376506
2211	44-45	-0.21676518660565236
2211	46-47	0.49094627854578476
2211	48-49	0.41204480573582103
2211	50-51	-4.8012319839342155
2211	52-53	-1.116785153140814
2211	54-55	-0.8335639793418927
2211	56-57	-6.940319461614184
2211	58-59	-2.4476281052423374
2211	60-61	-1.3619621294236843
2211	62-63	-0.6304332132165271
2211	64-65	-3.7631430606963683
2211	66-67	-2.6423259797082324
2211	68-69	0.12570399580725677
2211	70-71	-0.19914066016795573
2211	72-73	-1.1774193479521244
2211	74-75	-1.7224221758231621
2211	76-77	-1.7862806087850664
2211	78-79	-1.3604740810068314
2211	80-81	-1.0630927402496546
2211	82-83	-1.5620873369900927
2211	84-85	-1.8153161097080428
2211	86-87	-2.191278566687835
2211	88-89	-2.6288757228440467
2211	90-91	-2.4141236217156994
2211	92-93	-2.266736939414674
2211	94-95	-2.4281686892345427
2211	96-97	-3.043096857129555
2211	98-99	-3.08977091971855
2211	100	-2.471322094768162
2212	1	-21.00491376717033
2212	2	-16.941482408628243
2212	3	-19.68335869147857
2212	4	-18.67565681870823
2212	5	-20.898409490855197
2212	6	-21.70946894048488
2212	7	-22.139033823416046
2212	8	-22.554342337724858
2212	9	-23.82989514304942
2212	10-11	-11.21560808790614
2212	12-13	-22.004951011697134
2212	14-15	-23.307957279937238
2212	16-17	-23.7430982350717
2212	18-19	-18.447943250475987
2212	20-21	-23.503674722449286
2212	22-23	-18.515099016036586
2212	24-25	-25.06156396974206
2212	26-27	-17.101438027281148
2212	28-29	-17.93811574489327
2212	30-31	-6.4062559814018485
2212	32-33	-17.085598520332724
2212	34-35	-13.745147443106573
2212	36-37	-17.546799325167854
2212	38-39	-23.533721343058932
2212	40-41	-10.158089861041805
2212	42-43	-13.933375946485812
2212	44-45	-17.046748222498824
2212	46-47	-17.034970074815572
2212	48-49	-17.751549242764497
2212	50-51	-9.006144238067542
2212	52-53	-2.280270207137182
2212	54-55	-23.415748304450748
2212	56-57	-23.365754875257142
2212	58-59	-12.574016109685832
2212	60-61	-6.495712259934901
2212	62-63	-3.439324107719255
2212	64-65	-17.414962410595297
2212	66-67	-17.57186896939808
2212	68-69	-14.601311802869667
2212	70-71	-9.632521855453184
2212	72-73	-17.448013195159465
2212	74-75	-6.243975221696328
2212	76-77	-11.228050735966484
2212	78-79	-4.657655414939658
2212	80-81	-2.4691223115050143
2212	82-83	-3.1180332634880585
2212	84-85	-5.491041469895485
2212	86-87	-5.175647908262267
2212	88-89	-3.684487258436686
2212	90-91	-4.3362659198607005
2212	92-93	-5.509562114581893
2212	94-95	-3.956287272432977
2212	96-97	-5.578020718530876
2212	98-99	-4.025895554875028
2212	100	-3.9591930170568475
2213	1	-21.00491376717033
2213	2	-16.941482408628243
2213	3	-19.68335869147857
2213	4	-18.67565681870823
2213	5	-20.898409490855197
2213	6	-21.70946894048488
2213	7	-10.49716531130532
2213	8	-10.301747182015516
2213	9	-4.703020863926007
2213	10-11	-17.46855569293267
2213	12-13	-16.154316640301516
2213	14-15	-23.307957279937238
2213	16-17	-17.771644947874467
2213	18-19	-18.443486990445386
2213	20-21	-17.64496653329127
2213	22-23	-19.653697560940426
2213	24-25	-7.755623946674007
2213	26-27	-23.113427657870908
2213	28-29	-23.99968411819204
2213	30-31	-19.33868774713929
2213	32-33	-23.19966203297044
2213	34-35	-17.764810788601835
2213	36-37	-23.577583511817238
2213	38-39	-11.484990085850168
2213	40-41	-16.966865550037237
2213	42-43	-23.10997996463228
2213	44-45	-17.147510604383452
2213	46-47	-16.912247633698527
2213	48-49	-6.283425536417944
2213	50-51	-23.30232051804162
2213	52-53	-17.75577329770283
2213	54-55	-17.66026964239769
2213	56-57	-17.51166029624907
2213	58-59	-18.047650620865166
2213	60-61	-19.267148130230563
2213	62-63	-17.57651248160108
2213	64-65	-17.65023223428094
2213	66-67	-8.699258280503557
2213	68-69	-22.69269223060784
2213	70-71	-17.340660556780232
2213	72-73	-9.56724534152722
2213	74-75	-4.5925193780268465
2213	76-77	-3.38963024711218
2213	78-79	-4.934285147309904
2213	80-81	-3.661914143650936
2213	82-83	-3.6877201180629093
2213	84-85	-3.493683157884778
2213	86-87	-3.568370259678531
2213	88-89	-3.25204727012769
2213	90-91	-3.2365132323879386
2213	92-93	-4.032193621532585
2213	94-95	-4.0351905084633835
2213	96-97	-3.6385145512284502
2213	98-99	-3.999034842994682
2213	100	-3.491922215632698
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	588.0
3	741.0
4	5987.0
5	15932.0
6	16588.0
7	20087.0
8	19672.0
9	20531.0
10	23047.0
11	26858.0
12	33323.0
13	44443.0
14	36407.0
15	38958.0
16	42600.0
17	43965.0
18	43169.0
19	42877.0
20	41649.0
21	39913.0
22	40983.0
23	43737.0
24	47099.0
25	51096.0
26	54635.0
27	58878.0
28	62908.0
29	65684.0
30	68541.0
31	73445.0
32	82262.0
33	99871.0
34	120586.0
35	142856.0
36	161326.0
37	149501.0
38	52637.0
39	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.403353280796615	21.423598467411892	13.413867494795568	23.759180756995928
2	14.744223872060132	21.59262029489867	40.964842935020954	22.69831289802024
3	17.973727724194084	27.475413310133874	29.978111084156605	24.57274788151544
4	12.2516693537906	17.77948723352688	39.8823285597367	30.08651485294583
5	12.450970288146177	38.70713120866196	36.13109347624446	12.710805026947398
6	30.001707873767586	39.15955003688655	18.364279497356904	12.474462591988956
7	25.34100482259062	33.160482876316955	24.123126373821073	17.375385927271356
8	23.65267603395977	37.37070972582953	21.985072869932402	16.9915413702783
9	24.03592954532155	17.577455209519087	22.65939581569077	35.72721942946859
10-11	22.88689899727342	28.256373480160384	29.370592215621215	19.48613530694498
12-13	23.573457966336395	25.911414099500302	29.43843443895753	21.076693495205774
14-15	20.81014725055668	27.427662982435503	26.91677393864831	24.845415828359513
16-17	20.141946413139404	31.332692702389142	27.840452552233035	20.68490833223842
18-19	20.14936980235825	30.139799485753123	29.7095696159963	20.001261095892332
20-21	21.65140036382605	29.57864546084919	28.569105476916167	20.200848698408592
22-23	20.459501189300468	29.516991000264287	29.321111126566606	20.702396683868635
24-25	20.83123054344189	29.291347843227765	28.794546476533966	21.082875136796375
26-27	20.837419210404498	29.133022554041432	28.957188947668516	21.072369287885554
28-29	20.5355511412557	29.16038356466435	29.45741141140973	20.846653882670225
30-31	21.210168398812073	29.619046087938184	28.682404162053714	20.488381351196033
32-33	20.26357958717974	29.401105385919106	29.39691809927219	20.938396927628958
34-35	20.42549533042249	28.75836438796337	29.325365323773685	21.490774957840458
36-37	20.954441360294066	29.497255219238994	28.863661613013473	20.684641807453467
38-39	20.736081404892328	29.209594211963562	29.05878950056814	20.995534882575974
40-41	20.89503005950987	29.226095843906048	28.772107165464217	21.10676693111986
42-43	20.55863028954793	29.388647281474306	29.26079363612903	20.791928792848733
44-45	21.126485314305413	28.828018191848987	29.08392893998793	20.96156755385767
46-47	20.99330344086489	29.368653908610153	29.144237566577036	20.493805083947922
48-49	20.78023588033454	29.124595830429982	29.224970440873456	20.87019784836202
50-51	21.067415257352838	29.184400606401113	28.858159726412293	20.890024409833757
52-53	20.472706846219094	29.248633404606178	29.074089340377906	21.204570408796826
54-55	20.999730133790724	29.315610463303436	28.67935534536528	21.005304057540553
56-57	21.068695778980473	29.20411178644244	28.626414595961403	21.100777838615684
58-59	20.745272525027808	28.6560756200942	29.25925163370894	21.339400221169054
60-61	21.259888470838607	29.20390736572964	28.596657288487147	20.939546874944607
62-63	20.63165069400761	29.353280253803593	29.04409655133211	20.970972500856682
64-65	20.686729400653586	28.760426658000167	29.124490629435606	21.42835331191064
66-67	20.821277334221755	29.08466712587481	28.82336305331278	21.270692486590658
68-69	21.33973562509081	28.963040880490983	28.732664449822963	20.96455904459524
70-71	21.38850145642796	29.066433288589455	28.473989376517196	21.071075878465386
72-73	21.02270628135691	29.23508933783286	28.931271038750882	20.810933342059347
74-75	21.35448504844288	28.709470436027768	28.59943246049624	21.336612055033118
76-77	21.253371882632504	29.24641999104506	28.654506630384972	20.84570149593747
78-79	21.296527577856427	28.959130666711825	28.843420306176014	20.900921449255726
80-81	21.401108897996707	28.91098811302205	28.588942727152787	21.09896026182846
82-83	20.84771523764016	29.200159714085604	28.983576940951604	20.96854810732263
84-85	21.09259672067811	28.781140885671146	28.579963243760503	21.54629914989024
86-87	21.325139192669507	29.062893386143024	28.59596891576209	21.01599850542538
88-89	21.129202342881378	28.666769826805588	28.904637308749958	21.299390521563076
90-91	21.44586984768792	28.710271828699764	28.563263065254663	21.280595258357653
92-93	20.968709124620013	29.03461941526916	28.878412078439126	21.11825938167171
94-95	20.928865403948674	28.57607812500814	28.82394511903713	21.67111135200605
96-97	21.152628988632358	28.785016860645772	28.774042321576417	21.288311829145456
98-99	21.129194434701272	28.59230679293815	28.810216038556973	21.468282733803605
100	21.328467000944396	28.43705160782022	28.563110139467902	21.67137125176748
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	303.0
1	357.0
2	360.5
3	313.5
4	382.0
5	569.0
6	912.5
7	1434.5
8	2092.0
9	2830.0
10	3697.0
11	4495.0
12	5199.0
13	5935.0
14	6545.5
15	7228.0
16	8024.5
17	8723.0
18	9514.0
19	10416.5
20	11400.0
21	12681.5
22	14124.5
23	16028.0
24	18618.0
25	21808.0
26	25507.5
27	29639.5
28	34613.0
29	39796.5
30	44570.0
31	49480.0
32	54352.5
33	58514.5
34	62152.0
35	65672.0
36	68542.5
37	70477.0
38	71826.0
39	72210.0
40	71693.5
41	70121.5
42	67724.0
43	65150.0
44	62151.0
45	58785.0
46	55661.0
47	53249.5
48	51243.5
49	49823.5
50	49188.0
51	46209.5
52	40732.5
53	36042.0
54	32587.0
55	29531.5
56	26744.0
57	24079.0
58	21291.0
59	18662.5
60	15968.5
61	13440.5
62	11118.5
63	8977.0
64	7218.5
65	5706.0
66	4460.5
67	3492.0
68	2691.0
69	2050.5
70	1551.5
71	1225.0
72	969.5
73	747.0
74	574.5
75	437.0
76	314.5
77	214.0
78	152.5
79	108.0
80	66.5
81	48.5
82	43.0
83	31.5
84	21.0
85	17.5
86	10.5
87	6.0
88	7.5
89	7.0
90	2.5
91	2.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	9.6692712050593
2	27.1204024932404
3	7.2614178701537275
4	26.381191467706
5	14.689989594258845
6	11.878422464815976
7	9.509254489673905
8	10.811058220432077
9	6.31554013657794
10-11	8.01363607213268
12-13	12.005184251955473
14-15	9.220071081762645
16-17	9.662677109645067
18-19	7.72597835688589
20-21	9.676977245974758
22-23	7.047820897127973
24-25	6.33353813817914
26-27	11.289608533121495
28-29	7.585976660522522
30-31	5.098839025459932
32-33	10.453141065026573
34-35	8.562575120969326
36-37	8.524613818741505
38-39	7.5579711494103075
40-41	10.003191024996534
42-43	7.912733482696059
44-45	8.03646974370432
46-47	7.160256688740079
48-49	4.884155966130657
50-51	7.85545535978419
52-53	5.5821991489220855
54-55	6.285802059219632
56-57	6.016556092737289
58-59	4.546098674560962
60-61	5.17372726200746
62-63	3.558173885520297
64-65	5.1546173149050345
66-67	3.442686708579254
68-69	6.385515125561921
70-71	3.349464300860387
72-73	2.0082252936053306
74-75	1.1814550246489872
76-77	1.471646941270659
78-79	2.271265052638983
80-81	2.7847252822272837
82-83	1.2353197334640775
84-85	1.744694209815324
86-87	0.6170521755860211
88-89	0.7345046515524828
90-91	0.3336870871579087
92-93	0.5576794576602184
94-95	0.7642427289107899
96-97	0.8001870137177872
98-99	0.4718269212866709
100	0.878074917198849
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1933548.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.19138676816067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.39200408405866	95.62855323828188
2	1.3462991218015106	2.6169735730529133
3	0.14316512465665387	0.4174325100665035
4	0.04336405829097113	0.16858451844779357
5	0.02244687175059307	0.10908212970235955
6	0.011630191329844537	0.06782126542359776
7	0.010394674432352624	0.07071909761587263
8	0.008561809891792423	0.0665707341302929
9	0.0036222903014513064	0.031684987590742016
>10	0.01581056546933047	0.24944982740420293
>50	9.90451324373978E-4	0.06976833201241192
>100	0.0014436117342297601	0.2695263005142891
>500	1.60286974935965E-4	0.08874559785900613
>1k	1.0685798329057452E-4	0.14508788789814048
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.03436790811503E-4	0.0
8	0.0	0.0	0.0	1.03436790811503E-4	0.0
9	0.0	0.0	0.0	1.551551862172545E-4	0.0
10-11	0.0	0.0	0.0	2.3273277932588175E-4	0.0
12-13	0.0	0.0	0.0	2.585919770287575E-4	0.0
14-15	0.0	0.0	0.0	2.585919770287575E-4	0.0
16-17	0.0	0.0	0.0	3.3616957013738477E-4	0.0
18-19	0.0	0.0	0.0	3.620287678402605E-4	0.0
20-21	0.0	0.0	0.0	4.13747163246012E-4	0.0
22-23	0.0	0.0	0.0	4.13747163246012E-4	0.0
24-25	0.0	0.0	0.0	4.913247563546393E-4	0.0
26-27	0.0	0.0	0.0	6.723391402747695E-4	0.0
28-29	0.0	0.0	0.0	0.0012412414897380358	0.0
30-31	0.0	0.0	0.0	0.0040857532370543685	0.0
32-33	0.0	0.0	0.0	0.011171173407642324	0.0
34-35	0.0	0.0	0.0	0.02066149896459772	0.0
36-37	0.0	0.0	0.0	0.03607358079551167	0.0
38-39	0.0	0.0	0.0	0.05859694199471645	0.0
40-41	0.0	0.0	0.0	0.08422340691826632	0.0
42-43	0.0	0.0	0.0	0.10809144639802062	0.0
44-45	0.0	0.0	0.0	0.13632969028956093	0.0
46-47	0.0	0.0	0.0	0.17248084867818125	0.0
48-49	0.0	0.0	0.0	0.21070074288303162	0.0
50-51	0.0	0.0	0.0	0.25005844178680847	0.0
52-53	0.0	0.0	0.0	0.29365704911385704	0.0
54-55	0.0	0.0	0.0	0.3424533551791835	0.0
56-57	0.0	0.0	0.0	0.396059472017245	0.0
58-59	0.0	0.0	0.0	0.45858701206279856	0.0
60-61	0.0	0.0	0.0	0.5231315695291765	0.0
62-63	0.0	0.0	0.0	0.5894345524393498	0.0
64-65	0.0	0.0	0.0	0.6662880880122966	0.0
66-67	0.0	0.0	0.0	0.7595622141265694	0.0
68-69	0.0	0.0	0.0	0.8658176574876859	0.0
70-71	0.0	0.0	0.0	0.9883902545993168	0.0
72-73	0.0	0.0	0.0	1.1166518752055805	0.0
74-75	0.0	0.0	0.0	1.2522057895640555	0.0
76-77	0.0	2.585919770287575E-5	0.0	1.408809090852671	0.0
78-79	0.0	5.17183954057515E-5	0.0	1.5802297124250342	0.0
80-81	0.0	5.17183954057515E-5	0.0	1.7723118329619951	0.0
82-83	0.0	5.17183954057515E-5	0.0	1.9780476098860749	0.0
84-85	0.0	5.17183954057515E-5	0.0	2.191696301307234	0.0
86-87	0.0	5.17183954057515E-5	0.0	2.425825477309071	0.0
88	0.0	5.17183954057515E-5	0.0	2.6266221474719016	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACT	115	0.0013152619	24.702736	4
CGTACTA	130	0.005258748	19.45656	5
GTATAGA	875	0.0	19.398949	1
GTCCTAG	600	0.0	18.860086	1
TACATCG	200	8.128014E-4	18.855627	2
GTACTGG	685	0.0	18.355316	1
TATACGT	230	2.0447186E-4	17.291914	4
GTATTAG	935	0.0	16.809347	1
GTGTAGG	565	6.002665E-11	16.690344	1
GTACTAG	340	5.9082067E-6	16.641254	1
CTAGTAC	380	1.4766738E-7	16.446821	3
GTATAGG	510	4.7039066E-9	16.024912	1
TATACTG	1285	0.0	15.353271	5
GTCTAGG	540	1.0017175E-8	15.134639	1
GGTATAC	380	2.6623075E-6	14.951654	3
GTATAGC	425	2.9753337E-6	14.792227	1
GTACATG	7275	0.0	14.776976	1
CCCTAGG	520	1.0342592E-7	14.50776	1
GTATAAA	1310	0.0	14.397014	1
TACATGG	7300	0.0	14.292395	2
>>END_MODULE