FastQCFastQC Report
Wed 25 May 2016
SRR1295098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933548
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44610.23071576190505744No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33470.17310146942305027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAC1100.0030588321.365393
CCCGTAC1250.00640967118.8015443
GGACCGT1250.00645486618.7786776
GTATAGC4001.891749E-1017.6156581
GAGTACT30650.017.15285312-13
GTATCAA79650.016.8716011
GAACCGA1750.002347094216.0960086
CGGGACT2355.6200835E-516.0013144
TCTATAC7000.015.4441253
CAACGCA85550.015.3291385
ACCGCTC2155.241633E-415.2825978
TCAACGC86200.015.2135474
ATCAACG86200.015.15901853
AACGCAG86150.015.0948736
TATCAAC88100.014.8994492
TACACTG13350.014.7877325
CCTATAC6050.014.7615433
CTTACAC10200.014.746313
CCGCTCA2257.4001553E-414.603379
GTATAGA8300.014.5533821
Produced by FastQC (version 0.11.5)