##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295098_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1933548 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97406994809542 33.0 33.0 33.0 27.0 33.0 2 29.430555641752882 33.0 33.0 33.0 15.0 33.0 3 30.408050899176022 33.0 33.0 33.0 22.0 33.0 4 34.73187942580169 37.0 33.0 37.0 27.0 37.0 5 35.1075344392795 37.0 37.0 37.0 33.0 37.0 6 34.75289467859086 37.0 37.0 37.0 27.0 37.0 7 34.6416054838049 37.0 37.0 37.0 27.0 37.0 8 34.628803112206164 37.0 37.0 37.0 27.0 37.0 9 34.58716463206499 37.0 37.0 37.0 27.0 37.0 10-11 34.57382956099357 37.0 37.0 37.0 27.0 37.0 12-13 34.45898834681115 37.0 37.0 37.0 27.0 37.0 14-15 35.801498850817254 37.0 37.0 40.0 27.0 40.0 16-17 35.79687445049205 37.0 37.0 40.0 27.0 40.0 18-19 35.84100601588375 37.0 37.0 40.0 27.0 40.0 20-21 35.84053537848556 37.0 37.0 40.0 27.0 40.0 22-23 35.80334209442951 37.0 37.0 40.0 27.0 40.0 24-25 35.7736479777073 37.0 37.0 40.0 27.0 40.0 26-27 35.75184324361226 37.0 37.0 40.0 27.0 40.0 28-29 35.690978967162955 37.0 37.0 40.0 27.0 40.0 30-31 35.5907026874947 37.0 37.0 40.0 27.0 40.0 32-33 35.544750375992734 37.0 37.0 40.0 27.0 40.0 34-35 35.431720598609395 37.0 37.0 40.0 27.0 40.0 36-37 35.423558401446456 37.0 37.0 40.0 27.0 40.0 38-39 35.358118598555606 37.0 33.0 40.0 27.0 40.0 40-41 35.29300488014779 37.0 33.0 40.0 27.0 40.0 42-43 35.18777552975153 37.0 33.0 40.0 27.0 40.0 44-45 35.073914896346 37.0 33.0 40.0 27.0 40.0 46-47 34.959014206008845 37.0 33.0 40.0 22.0 40.0 48-49 34.85660247379428 37.0 33.0 40.0 22.0 40.0 50-51 34.75559670615883 37.0 33.0 40.0 22.0 40.0 52-53 34.66514407710592 37.0 33.0 40.0 22.0 40.0 54-55 34.550837372540016 37.0 33.0 40.0 22.0 40.0 56-57 34.4208268943931 37.0 33.0 40.0 22.0 40.0 58-59 34.30175640842637 37.0 33.0 40.0 22.0 40.0 60-61 34.15189304842704 37.0 33.0 40.0 22.0 40.0 62-63 33.969700778051546 37.0 33.0 40.0 22.0 40.0 64-65 33.80261519238209 37.0 33.0 40.0 22.0 40.0 66-67 33.587256949400796 37.0 33.0 40.0 22.0 40.0 68-69 33.37917160577342 37.0 33.0 37.0 22.0 40.0 70-71 33.15602250370821 37.0 33.0 37.0 22.0 40.0 72-73 32.949475782344166 37.0 33.0 37.0 22.0 40.0 74-75 32.718755624375504 37.0 33.0 37.0 22.0 40.0 76-77 32.48549350727264 37.0 33.0 37.0 22.0 38.5 78-79 32.258769112533024 37.0 33.0 37.0 22.0 37.0 80-81 32.04275663184984 37.0 33.0 37.0 22.0 37.0 82-83 31.836327569835348 33.0 33.0 37.0 22.0 37.0 84-85 31.659776483438733 33.0 33.0 37.0 22.0 37.0 86-87 31.66271538125767 33.0 33.0 37.0 22.0 37.0 88-89 31.673457033391465 33.0 33.0 37.0 22.0 37.0 90-91 31.674143595090477 33.0 33.0 37.0 22.0 37.0 92-93 31.629913247563547 33.0 33.0 37.0 22.0 37.0 94-95 31.647414752568853 33.0 33.0 37.0 22.0 37.0 96-97 31.601160664229695 33.0 33.0 37.0 22.0 37.0 98-99 31.517116202959535 33.0 33.0 37.0 22.0 37.0 100 30.97912697279819 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7297878441299943 1101 2 4.161716396287115 1101 3 2.4419622241457226 1101 4 1.2844868667107292 1101 5 0.601904171496308 1101 6 0.7239929122296047 1101 7 0.6895753339544086 1101 8 0.6892610342748213 1101 9 0.7109211119732493 1101 10-11 0.7417707582183439 1101 12-13 0.7908313447191944 1101 14-15 1.0282117234802826 1101 16-17 1.0522217798588827 1101 18-19 1.0688542648329218 1101 20-21 1.1524411842057276 1101 22-23 0.973097500874168 1101 24-25 0.9930765991921646 1101 26-27 1.0351633448988977 1101 28-29 0.9722522547381871 1101 30-31 0.8445232410887655 1101 32-33 1.0093449786185218 1101 34-35 1.1146780214988894 1101 36-37 1.0714047476721191 1101 38-39 1.013270147895149 1101 40-41 1.0869660122020193 1101 42-43 1.1179296328509878 1101 44-45 1.160081483815425 1101 46-47 1.0478277252031774 1101 48-49 0.9181489001582932 1101 50-51 1.0979956340204566 1101 52-53 1.0156773404789092 1101 54-55 1.0618610076144677 1101 56-57 1.0370723247673084 1101 58-59 1.061642616318764 1101 60-61 1.0219983241832153 1101 62-63 1.0778427908038282 1101 64-65 1.0468201295011639 1101 66-67 1.0094369610774194 1101 68-69 1.0008498451366705 1101 70-71 1.0451553211013263 1101 72-73 0.8526258763472114 1101 74-75 0.8859042318568413 1101 76-77 0.9696221471772901 1101 78-79 0.9805938059131947 1101 80-81 1.012379565384446 1101 82-83 1.100419516265724 1101 84-85 0.9664021353519843 1101 86-87 1.033938700304315 1101 88-89 0.9413030244742906 1101 90-91 0.9575008747674048 1101 92-93 0.9025551506294356 1101 94-95 0.9604808520863735 1101 96-97 0.8548778480056107 1101 98-99 0.9143750735932379 1101 100 1.014382344991951 1102 1 0.493871973135505 1102 2 4.043850481306265 1102 3 2.268091054604323 1102 4 1.1576181280730466 1102 5 0.5990343959309072 1102 6 0.582375414086755 1102 7 0.5464339958961375 1102 8 0.6006498950626948 1102 9 0.6770801873967187 1102 10-11 0.7312199504957846 1102 12-13 0.7013228737487083 1102 14-15 0.9103260034775502 1102 16-17 0.8862863183194278 1102 18-19 0.9130102212908753 1102 20-21 0.8409975450208691 1102 22-23 0.8233313674987812 1102 24-25 0.8089397026657466 1102 26-27 0.8501699664088775 1102 28-29 0.9579198965929763 1102 30-31 0.8839246967547751 1102 32-33 0.9086904644868454 1102 34-35 1.0257146380399433 1102 36-37 0.8202182130042175 1102 38-39 0.9011259936185496 1102 40-41 0.7334200578565842 1102 42-43 0.9821064208446444 1102 44-45 0.9485392100427035 1102 46-47 1.0470138906732274 1102 48-49 0.932478561845123 1102 50-51 0.9223629952947974 1102 52-53 0.9043657719658071 1102 54-55 0.7763505413887515 1102 56-57 0.7594939402719376 1102 58-59 0.7906367406429737 1102 60-61 0.8394710583399387 1102 62-63 0.891161212412392 1102 64-65 0.8036982059432205 1102 66-67 0.8511621377915262 1102 68-69 0.8165032447322176 1102 70-71 0.758671030481878 1102 72-73 0.7778182175127952 1102 74-75 0.7654859795486715 1102 76-77 0.7139566401736914 1102 78-79 0.7561252685614832 1102 80-81 0.6077075514238608 1102 82-83 0.7295802445252022 1102 84-85 0.7177463043568224 1102 86-87 0.6795937884406946 1102 88-89 0.7591263602077838 1102 90-91 0.683074278191306 1102 92-93 0.8346481936924413 1102 94-95 0.7369729977680244 1102 96-97 0.661462841117558 1102 98-99 0.8242866161211531 1102 100 0.8579578810354818 1103 1 0.6659337261235514 1103 2 4.078462035091125 1103 3 2.3410492219349983 1103 4 1.1449189248857934 1103 5 0.5390244357715446 1103 6 0.5079232228516943 1103 7 0.5446909680076928 1103 8 0.6327714090069136 1103 9 0.7104467212612633 1103 10-11 0.6901342931252685 1103 12-13 0.6893706825136476 1103 14-15 1.024992338138901 1103 16-17 0.9394486689170449 1103 18-19 1.0785978706932724 1103 20-21 1.0100712502001556 1103 22-23 0.8482317659051546 1103 24-25 0.8277395034625528 1103 26-27 0.8348562213889537 1103 28-29 0.8674069483858062 1103 30-31 0.8612653342049725 1103 32-33 0.9242532134908359 1103 34-35 1.073149897004086 1103 36-37 1.0115777747572068 1103 38-39 0.9566538820647636 1103 40-41 0.9017467510836852 1103 42-43 0.9761303252271105 1103 44-45 0.9411935925128248 1103 46-47 0.8510477552150633 1103 48-49 0.9535193984985071 1103 50-51 0.8612325172071493 1103 52-53 0.8798388795355265 1103 54-55 0.9538903820262092 1103 56-57 0.9065307928615738 1103 58-59 0.8584903263003412 1103 60-61 0.827892373080978 1103 62-63 0.985409220380518 1103 64-65 0.9352968115208995 1103 66-67 0.9396830541261849 1103 68-69 1.0020112128597134 1103 70-71 0.9698264089679327 1103 72-73 0.9658162254809213 1103 74-75 0.9267160592299462 1103 76-77 0.790774369257349 1103 78-79 0.8874748701551098 1103 80-81 0.8804914159656967 1103 82-83 0.9348840293857634 1103 84-85 1.0121935155122017 1103 86-87 0.9534981709108159 1103 88-89 0.9113922964627612 1103 90-91 0.9481390192271562 1103 92-93 0.9993643291506054 1103 94-95 0.8220078583257937 1103 96-97 0.865272602073734 1103 98-99 0.8689828312605989 1103 100 0.8686650523502202 1104 1 0.39635343095203623 1104 2 3.6873315883247315 1104 3 2.1168809892055407 1104 4 0.957643122435222 1104 5 0.4041555465485587 1104 6 0.35495979355003726 1104 7 0.3736166489317938 1104 8 0.3251810158625119 1104 9 0.48626289054638505 1104 10-11 0.44890891163237256 1104 12-13 0.4426369257169327 1104 14-15 0.5346217450396011 1104 16-17 0.47356362419028386 1104 18-19 0.5277155195945227 1104 20-21 0.5963854587746624 1104 22-23 0.5270966835928945 1104 24-25 0.5516329234931305 1104 26-27 0.507816643850056 1104 28-29 0.6481094841546735 1104 30-31 0.62085590274841 1104 32-33 0.6782510899096863 1104 34-35 0.7076008353889023 1104 36-37 0.580804703520144 1104 38-39 0.5819295717588204 1104 40-41 0.5995941351323921 1104 42-43 0.639546534603447 1104 44-45 0.6577523261318063 1104 46-47 0.6532941156851351 1104 48-49 0.5163910878417681 1104 50-51 0.38747778769751307 1104 52-53 0.5062069293488562 1104 54-55 0.5647235587532649 1104 56-57 0.5298518795838731 1104 58-59 0.49796889003283695 1104 60-61 0.5239507809588986 1104 62-63 0.5096779630200174 1104 64-65 0.4039619935600456 1104 66-67 0.3632656420136371 1104 68-69 0.5061052455340445 1104 70-71 0.4040538701922145 1104 72-73 0.3063472773739804 1104 74-75 0.33529103605221877 1104 76-77 0.4385246887341623 1104 78-79 0.3239756594507597 1104 80-81 0.39191719085356524 1104 82-83 0.4573499205381282 1104 84-85 0.4706093490334595 1104 86-87 0.5414695244902035 1104 88-89 0.4826609326099991 1104 90-91 0.43949259862028 1104 92-93 0.41085647377963497 1104 94-95 0.3945646750839167 1104 96-97 0.5457719255346056 1104 98-99 0.4287397783151512 1104 100 0.32421198411999796 1105 1 0.4653925249887898 1105 2 3.810202617282048 1105 3 2.133961595515668 1105 4 1.016328945700451 1105 5 0.3935393457828624 1105 6 0.3163991885649651 1105 7 0.3101751811426112 1105 8 0.34367035148630976 1105 9 0.41065660407763005 1105 10-11 0.2485023071489323 1105 12-13 0.3151652843920587 1105 14-15 0.5168318524168711 1105 16-17 0.3934797667479373 1105 18-19 0.4653703506255624 1105 20-21 0.4358047033951493 1105 22-23 0.42799498041606654 1105 24-25 0.512561602075877 1105 26-27 0.48035967284523196 1105 28-29 0.4057203988727949 1105 30-31 0.36849800372753094 1105 32-33 0.4668679626706833 1105 34-35 0.5774800182047599 1105 36-37 0.4720935621219198 1105 38-39 0.4285880041168113 1105 40-41 0.39115046679520304 1105 42-43 0.5044406480192194 1105 44-45 0.44385044691113507 1105 46-47 0.5004109245592616 1105 48-49 0.6092544030501585 1105 50-51 0.456229816697423 1105 52-53 0.3259813850089941 1105 54-55 0.4253420783759978 1105 56-57 0.3158173049521693 1105 58-59 0.3832408351753003 1105 60-61 0.39875152488610155 1105 62-63 0.5267024435250605 1105 64-65 0.4307355169450062 1105 66-67 0.37325669785076343 1105 68-69 0.36702029491623733 1105 70-71 0.33694680998237914 1105 72-73 0.4769482399362701 1105 74-75 0.2948605804677982 1105 76-77 0.2926519230002427 1105 78-79 0.38817610334624675 1105 80-81 0.37659219594084803 1105 82-83 0.38002436720678645 1105 84-85 0.37622041518714155 1105 86-87 0.36063170724453286 1105 88-89 0.36376489785553545 1105 90-91 0.36246291251780605 1105 92-93 0.4216346159492339 1105 94-95 0.43722369425471186 1105 96-97 0.3736538625847352 1105 98-99 0.29941210488593484 1105 100 0.226567091047702 1106 1 0.5191368306604218 1106 2 3.93411776117345 1106 3 2.21426372920498 1106 4 1.059731744787598 1106 5 0.41659165084599437 1106 6 0.3479030218957533 1106 7 0.35734913415673475 1106 8 0.34225354964849686 1106 9 0.28982606498375674 1106 10-11 0.34663711279074505 1106 12-13 0.44061250218504 1106 14-15 0.40573087681661946 1106 16-17 0.3161880657538134 1106 18-19 0.38032749446216485 1106 20-21 0.3814258589524897 1106 22-23 0.29337999158867945 1106 24-25 0.2615688179396898 1106 26-27 0.4279139213848211 1106 28-29 0.26221858173377655 1106 30-31 0.2936455513492504 1106 32-33 0.37702350897720294 1106 34-35 0.4086798729972969 1106 36-37 0.39671087064848365 1106 38-39 0.3268531394100691 1106 40-41 0.3064113969982927 1106 42-43 0.4116801427636929 1106 44-45 0.3884698606483923 1106 46-47 0.32434340110407334 1106 48-49 0.18127551442039191 1106 50-51 0.18644833707153907 1106 52-53 0.2584579918160088 1106 54-55 0.2795305425900807 1106 56-57 0.19670874885765954 1106 58-59 0.20176330458001246 1106 60-61 0.26701945348895606 1106 62-63 0.31899042940502653 1106 64-65 0.1596356770178886 1106 66-67 0.12666855256741627 1106 68-69 0.24782030225595975 1106 70-71 0.2519290291348142 1106 72-73 0.18997119975092147 1106 74-75 0.15589714753122053 1106 76-77 0.21822017181085585 1106 78-79 0.20217159487264524 1106 80-81 0.16962658315654622 1106 82-83 0.19889330370715896 1106 84-85 0.1864843649665815 1106 86-87 0.23893766601984723 1106 88-89 0.24135814734495042 1106 90-91 0.14640822613654692 1106 92-93 0.26963765994999633 1106 94-95 0.27736635513440433 1106 96-97 0.13413770025736937 1106 98-99 0.23742552120275562 1106 100 0.3381141221117083 1107 1 0.5138891061882376 1107 2 3.6902799935692485 1107 3 2.143695496667668 1107 4 1.0233679815565822 1107 5 0.44638792386967197 1107 6 0.3783894757203612 1107 7 0.16034499380642586 1107 8 0.09988734348593908 1107 9 0.2552520220985315 1107 10-11 0.2068061519998281 1107 12-13 0.2544981123682746 1107 14-15 0.2881201143051584 1107 16-17 0.3223637026898203 1107 18-19 0.263322629409771 1107 20-21 0.34001195049950184 1107 22-23 0.29892133057860804 1107 24-25 0.27139985462846994 1107 26-27 0.27726378282226705 1107 28-29 0.28285992104198954 1107 30-31 0.3561722839197401 1107 32-33 0.26858200284062406 1107 34-35 0.46109107434523366 1107 36-37 0.26272906360873804 1107 38-39 0.25095944830895434 1107 40-41 0.2923032102396306 1107 42-43 0.3626761429468246 1107 44-45 0.367889606870456 1107 46-47 0.29340364123019214 1107 48-49 0.2826842275429087 1107 50-51 0.15524036397756902 1107 52-53 0.2223569942978756 1107 54-55 0.32679678760744935 1107 56-57 0.3053612387846698 1107 58-59 0.2913482951184818 1107 60-61 0.4666787333358471 1107 62-63 0.47271094217092013 1107 64-65 0.3101758861091284 1107 66-67 0.43669438966565366 1107 68-69 0.4164156925141498 1107 70-71 0.24164842008147502 1107 72-73 0.20774690543569108 1107 74-75 0.29788881002961887 1107 76-77 0.30699366739911227 1107 78-79 0.2457692804288243 1107 80-81 0.34123517939636727 1107 82-83 0.2859323497250088 1107 84-85 0.3234200062801804 1107 86-87 0.3939021690835496 1107 88-89 0.31996849192854526 1107 90-91 0.3016171325292767 1107 92-93 0.4138233824865445 1107 94-95 0.29598318041534455 1107 96-97 0.17269558473447333 1107 98-99 0.29679214872082227 1107 100 0.32622325441072775 1108 1 0.5273046093640694 1108 2 3.7614158250581973 1108 3 2.1643345867611252 1108 4 1.0289271365547705 1108 5 0.23710277805928115 1108 6 0.22299091609155397 1108 7 0.15475269300810623 1108 8 0.08425571089230033 1108 9 0.1361629468199581 1108 10-11 0.17173914681870173 1108 12-13 0.19904943116213758 1108 14-15 0.22827631831383144 1108 16-17 0.06402176548811411 1108 18-19 0.16074165812672447 1108 20-21 0.2073601387671573 1108 22-23 0.11974075091655578 1108 24-25 0.17516953929654733 1108 26-27 0.15725758217700303 1108 28-29 0.1431544022479514 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0.360020062613863 2210 92-93 0.44955058655802205 2210 94-95 0.275984801891898 2210 96-97 0.2424997481873561 2210 98-99 0.2023091522965217 2210 100 0.2085308127044172 2211 1 0.5656035519002707 2211 2 3.9880088984360036 2211 3 2.35262844929262 2211 4 1.1841162719272376 2211 5 0.5848328566598298 2211 6 0.4391833058867505 2211 7 0.4885852023909152 2211 8 0.46560191571737164 2211 9 0.4208637613736741 2211 10-11 0.31991036301118214 2211 12-13 0.24596542112090702 2211 14-15 0.270398232702874 2211 16-17 0.24275810895080951 2211 18-19 0.30428058974449357 2211 20-21 0.3979408530659043 2211 22-23 0.19187171987736207 2211 24-25 0.30143940631847954 2211 26-27 0.15307789203583155 2211 28-29 0.09504465590117661 2211 30-31 0.1587127000391675 2211 32-33 0.21719158337848654 2211 34-35 0.3856390550309001 2211 36-37 0.25434364885153826 2211 38-39 0.2775173801768176 2211 40-41 0.1811471104589515 2211 42-43 0.3758576134616973 2211 44-45 0.3315960610132791 2211 46-47 0.24906092748967978 2211 48-49 0.21220869790466423 2211 50-51 0.0892533077749178 2211 52-53 0.16430804207260508 2211 54-55 0.1071255906835944 2211 56-57 0.13965110551517057 2211 58-59 0.15890261040051712 2211 60-61 0.27265179692406605 2211 62-63 0.257498475840535 2211 64-65 0.12566252573227388 2211 66-67 0.1623536923280966 2211 68-69 0.14267515361338212 2211 70-71 0.16621158668955616 2211 72-73 0.1613521153033446 2211 74-75 0.11913235196900729 2211 76-77 0.05774820107087919 2211 78-79 0.1864430034275344 2211 80-81 0.13745178204853303 2211 82-83 -0.13192439005693402 2211 84-85 0.09198573993815273 2211 86-87 0.15948355448536589 2211 88-89 0.22189412080544813 2211 90-91 0.259719981300492 2211 92-93 0.36634994257499187 2211 94-95 0.3272763344936891 2211 96-97 0.04913186747945275 2211 98-99 0.25679009278055887 2211 100 0.03433293235415391 2212 1 0.8983210917614564 2212 2 4.368003480456778 2212 3 2.5647035353359655 2212 4 1.4037287923504778 2212 5 0.911109491530361 2212 6 0.7761324662410658 2212 7 0.6903607193329151 2212 8 0.5775196358016146 2212 9 0.527286136122882 2212 10-11 0.49715870681206553 2212 12-13 0.618483944307691 2212 14-15 0.7375613750179539 2212 16-17 0.6855874933987636 2212 18-19 0.7554482301110781 2212 20-21 0.7438615426918886 2212 22-23 0.7647381318864674 2212 24-25 0.6038329585469455 2212 26-27 0.4598221709413579 2212 28-29 0.5951632917743979 2212 30-31 0.7563654263832476 2212 32-33 0.7731945350484111 2212 34-35 0.8903298163416338 2212 36-37 0.7166316680627958 2212 38-39 0.544919581419002 2212 40-41 0.8134551507965568 2212 42-43 0.6753294780648886 2212 44-45 0.8327771287502443 2212 46-47 0.8158631533761422 2212 48-49 0.7117700156023616 2212 50-51 0.7189708767953675 2212 52-53 0.7678230736727798 2212 54-55 0.3689121646300748 2212 56-57 0.5906330907342436 2212 58-59 0.6179340807704747 2212 60-61 0.6376936733884975 2212 62-63 0.7152352984402839 2212 64-65 0.5754696748311119 2212 66-67 0.5526632461255261 2212 68-69 0.30342053022408066 2212 70-71 0.5518085097288576 2212 72-73 0.723800087182525 2212 74-75 0.6644778280974464 2212 76-77 0.5528232250332081 2212 78-79 0.5949760385211746 2212 80-81 0.6405149156158281 2212 82-83 0.5406431865355117 2212 84-85 0.7493600470529174 2212 86-87 0.6942518606237691 2212 88-89 0.7283732385402111 2212 90-91 0.440325167698731 2212 92-93 0.5899985518192317 2212 94-95 0.6850037038484267 2212 96-97 0.5246459641019641 2212 98-99 0.495427151194086 2212 100 0.47037632133778473 2213 1 -0.9145425234341573 2213 2 -25.488769040606087 2213 3 -14.535365534265114 2213 4 -6.55667952560291 2213 5 -2.4820736976463422 2213 6 -1.5914609589907158 2213 7 -1.6191708856158797 2213 8 -1.5251051585406543 2213 9 -1.5286778071533362 2213 10-11 -1.4873965006546257 2213 12-13 -1.503241510008074 2213 14-15 -1.8103261342230965 2213 16-17 -1.845659026024073 2213 18-19 -1.6110631794544616 2213 20-21 -1.7770434111084725 2213 22-23 -1.7448882568871866 2213 24-25 -1.808418000317019 2213 26-27 -1.8226627798839203 2213 28-29 -1.7772442622273417 2213 30-31 -1.9090237516660764 2213 32-33 -1.8451712512798437 2213 34-35 -1.719965702880117 2213 36-37 -1.892999130999236 2213 38-39 -1.9866251930993997 2213 40-41 -2.1165989836245487 2213 42-43 -1.8402147328336085 2213 44-45 -1.971844853472554 2213 46-47 -1.9710100343877741 2213 48-49 -1.983001961275768 2213 50-51 -2.072572918489506 2213 52-53 -2.028286117592458 2213 54-55 -2.0832969413446705 2213 56-57 -1.9157479985471824 2213 58-59 -2.0819325266936772 2213 60-61 -1.9790672164731475 2213 62-63 -1.756227893218142 2213 64-65 -1.794681343908131 2213 66-67 -1.748722780436367 2213 68-69 -1.9209883976946145 2213 70-71 -1.782549468146449 2213 72-73 -1.8368866021933883 2213 74-75 -1.8408074166473938 2213 76-77 -1.8670904230153127 2213 78-79 -1.9202538458918923 2213 80-81 -1.7977074092639533 2213 82-83 -1.854163213017177 2213 84-85 -1.7769362974160217 2213 86-87 -1.9125699958684415 2213 88-89 -1.853303246624403 2213 90-91 -1.7520923505647232 2213 92-93 -1.7374887200137508 2213 94-95 -2.010407577968248 2213 96-97 -1.8996817098596175 2213 98-99 -1.7384766043533482 2213 100 -1.7192264611804298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 18.0 5 34.0 6 29.0 7 31.0 8 41.0 9 50.0 10 367.0 11 1728.0 12 5272.0 13 9714.0 14 12954.0 15 14538.0 16 15034.0 17 15394.0 18 15898.0 19 16016.0 20 17403.0 21 18877.0 22 20813.0 23 21691.0 24 22288.0 25 22908.0 26 24615.0 27 27356.0 28 31550.0 29 37295.0 30 43744.0 31 53285.0 32 65428.0 33 85948.0 34 115763.0 35 160477.0 36 237815.0 37 396351.0 38 418653.0 39 4155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.18258768961168 21.139911461059512 9.978556730728586 30.698944118600224 2 12.550155572207974 22.817257169548803 36.64884956261574 27.983737695627486 3 15.305231626005664 27.214271380901845 29.00905485666764 28.47144213642485 4 10.088552056723795 18.235434373673026 38.06543676836973 33.61057680123345 5 10.954343863015529 38.898038201936366 35.64267290258978 14.504945032458332 6 29.43764522021724 40.28787886628614 17.47538048343778 12.799095430058838 7 25.272866118657962 33.544838204981744 23.439685929752237 17.742609746608057 8 23.79916572584025 37.81056326441068 20.87589473368317 17.514376276065892 9 24.08079361441114 16.83284809494564 22.34707747136559 36.73928081927763 10-11 22.596785063363512 28.211735152552325 29.242548303601573 19.94893148048259 12-13 23.234097551813825 25.753452228627072 29.710948773682087 21.301501445877015 14-15 20.785481872649466 27.233182428382243 26.838289033300246 25.143046665668052 16-17 19.886934504377447 31.14337702329499 27.810340314000687 21.159348158326875 18-19 19.91151999064656 29.829035907787926 30.133022655703968 20.126421445861542 20-21 21.11808070072612 29.216475982246987 29.321231060993064 20.344212256033828 22-23 20.209132413713547 29.1969065970576 29.673322102560086 20.920638886668772 24-25 20.389837864569348 28.95120728639512 29.521601417799154 21.13735343123638 26-27 20.35896148057839 28.891001600137383 29.599308736512175 21.150728182772053 28-29 20.01475830749681 28.8822095146855 30.034604170346974 21.068428007470715 30-31 20.781760322555865 29.370084381735595 29.29711718800317 20.55103810770537 32-33 19.680081054201118 29.317680025197557 30.084855556262387 20.917383364338946 34-35 19.87168231416628 28.767883857236875 30.154268269818836 21.206165558778007 36-37 20.5829848869658 29.312086832774447 29.377151417650243 20.72777686260951 38-39 20.264722410503865 29.25836399357305 29.708989995197634 20.76792360072545 40-41 20.410616900312817 29.042912825435707 29.53204910706897 21.01442116718251 42-43 20.165791435057436 29.16890151148783 29.730608313984792 20.934698739469937 44-45 20.595630912415753 28.701131206331297 29.589912166707137 21.11332571454581 46-47 20.50505413640517 29.439765861955074 29.41884220536892 20.63633779627083 48-49 20.395081948555696 29.191217780058583 29.490849138301183 20.92285113308454 50-51 20.608234986172597 29.12088139472086 29.093749062395567 21.17713455671097 52-53 20.138779494716616 29.34811764854996 29.17473381326227 21.338369043471154 54-55 20.57890388693201 29.2941993215962 28.843697605928813 21.283199185542976 56-57 20.692337833136985 29.110182039666725 28.920008328020558 21.277471799175732 58-59 20.18993922151817 28.72385878701668 29.59663778611147 21.489564205353677 60-61 20.765552912113023 29.18459697444079 28.812900045543994 21.236950067902193 62-63 20.018627320138506 29.46297865384635 29.275127742757796 21.243266283257345 64-65 20.05532414023208 28.780036753437717 29.45178924063156 21.712849865698644 66-67 20.392682981082043 29.138803923363945 29.02290422251501 21.445608873039003 68-69 20.637894901300218 29.02265565708545 29.070405689030892 21.269043752583443 70-71 20.657411064663243 28.904369212177084 28.82674341816816 21.611476304991513 72-73 20.25917000313752 29.167682976510505 29.241503929918206 21.331643090433772 74-75 20.725583065210547 28.56047616226992 28.985687558732014 21.728253213787514 76-77 20.432992357132697 29.12434207973929 29.063095684560373 21.379569878567644 78-79 20.488475179368525 29.01928135687205 29.238395326396276 21.25384813736315 80-81 20.586325250086833 28.849025114591615 28.932665417838145 21.63198421748341 82-83 20.01145662001262 29.167442510008378 29.184873123752187 21.636227746226815 84-85 20.23772947429619 28.658993902754414 28.984778610192148 22.11849801275725 86-87 20.383905085864388 28.985550349041066 28.90235358649942 21.72819097859513 88-89 20.139500217750886 28.598760113002886 29.285839158126453 21.97590051111978 90-91 20.41312809510522 28.62570022532896 29.01113610743379 21.950035572132034 92-93 19.812931173370842 29.0276574192529 29.24129002960755 21.91812137776871 94-95 19.775590052314048 28.491581394506895 29.472049609131556 22.260778944047498 96-97 20.08551219003835 28.73698847002535 29.171040154370864 22.006459185565436 98-99 20.017977486249887 28.55469751043364 29.205941776424925 22.221383226891547 100 20.07325138718391 28.44435032884944 29.082167574197577 22.40023070976908 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 55.0 1 46.5 2 39.0 3 38.0 4 35.0 5 43.0 6 82.5 7 146.0 8 217.5 9 261.5 10 263.5 11 240.5 12 267.0 13 335.5 14 408.5 15 528.0 16 675.0 17 858.0 18 1162.0 19 1616.5 20 2245.5 21 3258.0 22 4629.0 23 6388.5 24 8950.0 25 12338.5 26 16239.0 27 21224.5 28 27299.0 29 33689.5 30 39907.0 31 46247.5 32 52435.5 33 58035.0 34 63304.0 35 68037.0 36 72100.5 37 75342.5 38 77787.0 39 79241.0 40 79342.5 41 78532.0 42 76396.5 43 73290.0 44 70042.5 45 66595.5 46 63188.0 47 60333.5 48 57967.5 49 56989.5 50 61387.0 51 58212.5 52 46697.5 53 40711.0 54 37500.0 55 34241.0 56 31081.5 57 28300.0 58 25175.5 59 21895.5 60 18849.0 61 15759.0 62 12721.0 63 10029.5 64 7911.5 65 6268.0 66 4739.0 67 3495.5 68 2642.0 69 1994.5 70 1494.5 71 1105.0 72 805.5 73 594.5 74 441.5 75 327.5 76 212.5 77 116.0 78 66.5 79 47.0 80 28.0 81 14.0 82 5.0 83 4.5 84 4.0 85 1.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8962280739862678 2 6.551169146046543 3 0.0 4 0.005637305099226914 5 0.16689526197436008 6 0.0588555339717452 7 0.0378578654370101 8 0.043960636094888775 9 0.042460802628121984 10-11 0.04398649529259165 12-13 0.041943618674064466 14-15 0.045227736782329685 16-17 0.035478819248345526 18-19 0.0294277669858726 20-21 0.024178349852188828 22-23 0.024126631456783072 24-25 0.021256260511763866 26-27 0.014300136329690289 28-29 0.020661498964597725 30-31 0.025212717760303854 32-33 0.0013963966759552905 34-35 0.003387554899076723 36-37 0.004370204411786002 38-39 0.006542377018827565 40-41 0.0072405753568052095 42-43 0.008016351287891481 44-45 0.008766268021274878 46-47 0.01704121128619512 48-49 0.02616950807531026 50-51 0.022238910024473143 52-53 0.028626131857083452 54-55 0.02487654819016647 56-57 0.0163171537505146 58-59 0.015412081830913946 60-61 0.013058894839952255 62-63 0.01892893271850505 64-65 0.02045462538297472 66-67 0.021256260511763866 68-69 0.029220893404249597 70-71 0.028781287043300713 72-73 0.025471309737332616 74-75 0.026350522459230388 76-77 0.01929096148634531 78-79 0.015903406587268586 80-81 0.013938107561850028 82-83 0.008688690428166253 84-85 0.011171173407642324 86-87 0.009283451975332395 88-89 0.007550885729239718 90-91 0.007887055299377103 92-93 0.0045770779934090075 94-95 0.0017067070483897996 96-97 0.0011636638966294086 98-99 9.567903150064028E-4 100 0.10948784307397592 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1933548.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.19574619118383 #Duplication Level Percentage of deduplicated Percentage of total 1 92.8891488778288 82.85316947221905 2 5.873948810123288 10.478624944155262 3 0.7584298746842424 2.0294615581844115 4 0.19726453668141114 0.7038063018542648 5 0.08428454195694265 0.37589113061158214 6 0.052144446527924736 0.2790637690670719 7 0.03106172888115587 0.19394018598800605 8 0.020501052265465117 0.14628853236180966 9 0.014652561354057214 0.11762515292285125 >10 0.0727880771433829 1.0847437786843799 >50 0.0026903015738679033 0.17089096155961395 >100 0.0022705631731622113 0.4990992004599777 >500 2.909385022588203E-4 0.18871186732392684 >1k 5.236893040658408E-4 0.8786831446077897 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4461 0.23071576190505744 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3347 0.17310146942305027 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.17183954057515E-5 0.0 4 0.0 0.0 0.0 5.17183954057515E-5 0.0 5 0.0 0.0 0.0 5.17183954057515E-5 0.0 6 0.0 0.0 0.0 5.17183954057515E-5 0.0 7 0.0 0.0 0.0 5.17183954057515E-5 0.0 8 0.0 0.0 0.0 5.17183954057515E-5 0.0 9 0.0 0.0 0.0 2.585919770287575E-4 0.0 10-11 0.0 0.0 0.0 2.585919770287575E-4 0.0 12-13 0.0 0.0 0.0 3.10310372434509E-4 0.0 14-15 0.0 0.0 0.0 3.10310372434509E-4 0.0 16-17 0.0 0.0 0.0 3.10310372434509E-4 0.0 18-19 0.0 0.0 0.0 3.3616957013738477E-4 0.0 20-21 0.0 0.0 0.0 4.13747163246012E-4 0.0 22-23 0.0 0.0 0.0 4.654655586517635E-4 0.0 24-25 0.0 0.0 0.0 6.464799425718937E-4 0.0 26-27 0.0 0.0 0.0 8.27494326492024E-4 0.0 28-29 0.0 0.0 0.0 0.0020428766185271842 0.0 30-31 0.0 0.0 0.0 0.005999333867067174 0.0 32-33 0.0 0.0 0.0 0.015670673807942705 2.585919770287575E-5 34-35 0.0 0.0 0.0 0.030177683719256 5.17183954057515E-5 36-37 0.0 0.0 0.0 0.05277862251156941 5.17183954057515E-5 38-39 0.0 0.0 0.0 0.08564566279192448 5.17183954057515E-5 40-41 0.0 0.0 0.0 0.12246916032081956 5.17183954057515E-5 42-43 0.0 0.0 0.0 0.15662916048631842 5.17183954057515E-5 44-45 0.0 0.0 0.0 0.198340046381057 5.17183954057515E-5 46-47 0.0 0.0 0.0 0.2513514016719523 5.17183954057515E-5 48-49 0.0 0.0 0.0 0.3060436048135345 5.17183954057515E-5 50-51 0.0 0.0 0.0 0.3624942333989123 5.17183954057515E-5 52-53 0.0 0.0 0.0 0.4239615463386479 5.17183954057515E-5 54-55 0.0 0.0 0.0 0.4940658313111441 5.17183954057515E-5 56-57 0.0 0.0 0.0 0.5705573381162505 5.17183954057515E-5 58-59 0.0 0.0 0.0 0.6617885876119962 5.17183954057515E-5 60-61 0.0 0.0 0.0 0.7523216387697642 5.17183954057515E-5 62-63 0.0 0.0 0.0 0.8439149170333501 5.17183954057515E-5 64-65 0.0 0.0 0.0 0.9505323891623068 5.17183954057515E-5 66-67 0.0 0.0 0.0 1.0790526017455995 5.17183954057515E-5 68-69 0.0 0.0 0.0 1.2220539650425022 5.17183954057515E-5 70-71 0.0 0.0 0.0 1.3814500596830284 5.17183954057515E-5 72-73 0.0 0.0 0.0 1.5438975396524937 5.17183954057515E-5 74-75 0.0 0.0 0.0 1.7158870635743204 5.17183954057515E-5 76-77 0.0 0.0 0.0 1.913916799582943 5.17183954057515E-5 78-79 0.0 0.0 0.0 2.1314443706595334 5.17183954057515E-5 80-81 0.0 0.0 0.0 2.3712367109583004 5.17183954057515E-5 82-83 0.0 0.0 0.0 2.62444997486486 5.17183954057515E-5 84-85 0.0 0.0 0.0 2.887463874700809 5.17183954057515E-5 86-87 0.0 0.0 0.0 3.1740355036440784 5.17183954057515E-5 88 0.0 0.0 0.0 3.4150173670371773 5.17183954057515E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGGAC 110 0.00305883 21.36539 3 CCCGTAC 125 0.006409671 18.801544 3 GGACCGT 125 0.006454866 18.778677 6 GTATAGC 400 1.891749E-10 17.615658 1 GAGTACT 3065 0.0 17.152853 12-13 GTATCAA 7965 0.0 16.871601 1 GAACCGA 175 0.0023470942 16.096008 6 CGGGACT 235 5.6200835E-5 16.001314 4 TCTATAC 700 0.0 15.444125 3 CAACGCA 8555 0.0 15.329138 5 ACCGCTC 215 5.241633E-4 15.282597 8 TCAACGC 8620 0.0 15.213547 4 ATCAACG 8620 0.0 15.1590185 3 AACGCAG 8615 0.0 15.094873 6 TATCAAC 8810 0.0 14.899449 2 TACACTG 1335 0.0 14.787732 5 CCTATAC 605 0.0 14.761543 3 CTTACAC 1020 0.0 14.74631 3 CCGCTCA 225 7.4001553E-4 14.60337 9 GTATAGA 830 0.0 14.553382 1 >>END_MODULE