FastQCFastQC Report
Wed 25 May 2016
SRR1295097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423787
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC2408.6351065E-820.7396347
CATGGGG37750.018.744444
CCTATAC5904.0017767E-1115.2287033
GTGTTAG9400.015.1747351
TATACCG2000.003720467215.0209945
TAGTACG3051.1388405E-414.7302134
GTACATG103600.014.7263961
GTATTAG8050.014.6379021
CTATACG2707.569557E-414.5597254
TACATGG105700.014.2530232
ACCGTCC2851.6733079E-414.07860858
GTATAAC6204.8803486E-914.0041721
TATACTG10450.013.8950185
GTATAAT12100.013.8388341
GTCTATA6851.3569661E-913.5806891
GTCTAGG6407.625204E-913.5665431
GTCTAGA6901.5133992E-913.4822791
GTATCGG2800.00837896513.2896751
TATCGTT2250.00844815413.2733667
ACATGGG104900.013.0084553
Produced by FastQC (version 0.11.5)